UniversityofHouston

BCHS3304Honors:GeneralBiochemistryI
MolecularDesignofLife
Fall2015

Dr.W.R.Widger
330403#22526TueThur4:005:30
announced)

RoomSEC204Aonehourrecitationperiod(placeandtimetobe

Officehours:13:30TueThursday
Emailwrwidger@central.uh.edu
Disclaimer:Thescheduleandtopicsbelowaresubjecttochangeintheeventofextenuatingcircumstances,
including,butnotlimitedto,instructorillnessorclasscancellationsduetoinclementweather.
Requiredmaterials:1)Voet,D.,Voet,J.,andPratt,C.,FundementalsofBiochemistry:LifeattheMolecular
Level(4thedition)ISBN#9780470547877or9871118129180)andUzman,J.A.,Johnson,J.,Eichberg,J.,
andWidger,W.,Voet,D.,Voet,J.,andPratt,C.AStudentCompaniontoBiochemistry.4theditionISBN#9781
118218273Thisstudyguideisextremelyimportant!!itisdesignedtoguideyouthroughcomplicated
subjectmatter.IthelpswithquestionslikeWhatamIexpectedtoknow?Whatsimportantinordertohavea
goodgraspofthissubject,andwhichisfundamental(obviously)tobiochemistrybuttobiologyaswell?
AlllecturesandhomeworkproblemssetswillbepostedonBlackboardLearn
ExamGradesandhomeworkgradesalsowillbepostedonBlackboardLearn
Lectureformat:Mostmaterialwillbecoveredbylectures,however,Somematerialmaynotandituptoyouto
coveritforyourselfasnotedinthesyllabus.Thestudentwillstillberesponsibleforthismaterial.Consultthe
studyguidetofindouttheconcepts,terms,reactions,etc.youareexpectedtoknow.
Homework:Allhomeworkistobeturnedinatthetimeitisdue.Iassignelevenhomeworksetsandwillgrade
ten.Ifyouhandinalleleven,Iwilldropthelowestgrade.Eachhomeworkisworth10pointsforatotalof100
points.
Examformatandgradingpolicy:Therewillbefourexamsworth100pointseach.Eachexamwillcovera
sectionofthematerial.Thelastexamwillbegiventhedateofthefinalexam(seeschedule).Totalpointsare
calculatedasfollows:100ptsforhomeworkand400pointsforexamgradesforatotalof500points.All
studentsareexpectedtotakeallexams.Nomakeupexamswillbegiven.Examsareclosedbook.Calculators
shouldbebroughttotheexams.
Gradingscale
85%andaboveA(425points)
75%to85%B(375424points)
55%to75%C(275374points)
45%to55%D(225274points)
below45%F(below225points)
Droppolicy:Studentswhohavenotturnedinanywork(examsorhomework)andhavenotstatedtheir
intentiontoremainintheclasswillbedroppedfromthemidtermclassrolls.Studentsmaydropanytime

beforethesecondexamination,regardlessofgradeonthefirstexam,andwillreceiveagradeofW.Ifthe
secondexamisturnedin,thestudentmusthaveapassinggradeononeoftheseexamstogetaW.
Homework:Problemsareassignedbothfromthetext(seebelow)andfromthestudyguideandafewthatare
notfoundinthetextofstudyguide.Studentsaretodoallassignedproblems.Pointdeductionswillbeassessed
forquestionsnotanswered.Studentsareencouragedtoworkingroupsaslongasallcontribute.Copying
homeworkanswersfromotherstudents,outofthehomeworkanswersolutionsupplement,orfromthe
studyguideconstitutesacademicdishonestyandwillbegradedappropriately.Homeworkisusuallydue
oneweekaftertheassignmentunlessotherwisenoted.

Homeworkcannotbereturned!!Pleasemakeaphotocopyforyourstudypurposes.

Date

Lecture

8/25

Chapter#
1

SectionandTopic

Homeworkdue

MolecularBiologicalStructures,ProcessesandmolecularEvolution
Distancetime,energyordersofmagnitude.
1.Theoriginoflife
2.CellularArchitecture
3.OrganismalEvolution

Homework:
8/27

Homework#1part1due
1

9/1

ThermodynamicsI
A.EnergyandtheFirstLawofThermodynamics
B.EntropyandtheSecondLawofThermodynamics
C.FreeEnergyandSpontaneityofProcesses
D.ChemicalEquilibrium

Homework:
8/28
9/1

Homework#2due

Recitation1
3

9/3

BasicBiochemicalcalculations

ThermodynamicsII
A.FreeEnergyandSpontaneityofProcesses
B.ChemicalEquilibrium
C.Coupledreactions

9/3

PhysicalpropertiesofmoleculesandthechemistryofWater
A.Fitnessofthemoleculesoflife
B.PhysicalpropertiesofH2O
C.Dissociation,Equilibrium,Acids,Bases,Buffers

Homework:

Recitation2Thermodynamiccalculations

9/4
9/8

Homework#3due

AcidsandBases
A.WaterIonization
B.HendersonHasselbalchequation
C.Buffersandtitrationcurves

9/10

AminoAcidsPart1
1.StructuresofAminoAcids
2.MolecularAsymmetryanditsDescription

Recitation3Buffercalculations,titrations

9/11
9/15

Aminoacidspart2

9/10

1.Steriochemistry
2.AminoAcidDerivatives
Homework:

Homework#4due
EXAMI

9/17
9/18
9/22

9/17

Recitation4
7

Protein:Primarystructure
A.Proteinpurification
i.Isolationofproteins
ii.Solubilityofproteins
iii.Chromatographicseparation
iv.Electrophoresisofproteins

9/24

Homework:

Homework#5due

ProteinSequencing

9/28

A.DeterminationofPrimaryCovalentStructureofProteins
B.ProteinModification
C.Massspectroscopyofproteins
Recitation5SequencingandMassspectroscopy

9/25
9/29

ProteinFamiliesandChemicalEvolutionofProteins
Proteinsequencedatabase

10/1

10

ProteinsThreedimensionalStructures
A.SecondaryStructureofProteins
B.TertiaryStructure

Recitation6ProteinStructureandthecomputer

10/2
10/6
10/8

11

StructuresofSomeFibrousProteins

Homework:

Homework#6due

12

TertiaryandQuarternaryStructureandSymmetry

10/13

A.Structuredetermination
B.Quarternaryproteinstructure
C.ProteinFoldingandMisfoldingDiseases
Recitation7Moreproteinfoldingandproblems

10/9

EXAM2

10/13
10/15

13

ProteinFunction

A.HemoglobinFunction
B.StructureandMechanismofHemoglobinandMyoglobin
Recitation8Bindingconstantsandhowtheyaremeasured

10/16
10/20

14

HemoglobinfunctionIIandMusclecontraction
A.AbnormalHemoglobins
B.AllostericRegulation
C.Musclecontraction
iThickandThinfilaments
iiMusclecontractionandmyosinmovement
iiiActinandmicrofiliments

Homework:
10/22

15

Homework#7

10/27

IntroductiontoEnzymes
A.Enzymes:Generalproperties
B.Specificityofbinding
C.Coenzymesandvitamins
D.ActivationEnergy

Recitation9Moreonproteinfunction

10/23

Homework:
10/27

16

11

Homework#8due

11/3

Enzymemechanisms
A.GeneralCatalyticMechanisms
B.AcidBasecatalysis
C.Covalentcatalysis
D.Metalioncatalysis

10/29

17

11

EnzymemechanismsII
A.Lysozyme
B.SerineProteases

Recitation10Enzymespecificityandmechanisms

10/30
11/3

18

12

EnzymeKineticsI
A.ChemicalKinetics
B.Enzymekinetics
C.KmandVmax
D.Bisubstratereactions

11/5

Homework:

Homework#9due

18cont. 12

EnzymeKineticsII

11/10

A.InhibitionofEnzymeCatalyzedReactions
B.Controlofenzymeactivity:Allostericregulation
C.DrugDesign
Recitation11ProblemsinenzymekineticsExamreview

11/6

EXAMIII

11/10
11/12

19

Carbohydrates
A.Monosaccharides
B.Polysaccharides

Homework:

11/4

Recitation12ProblemsincarbohydratesandLipids

11/13
11/17

Homework#10

20

LipidsandBiologicalmembranes
A.LipidClassification
B.LipidBilayers

11/19

21

Membranes
A.Membraneproteins
B.Membranestructureandassembly

Recitation13Topicsonmembranesandmembraneproteins

11/20
11/24

22

10

MembraneTransport
A.Thermodynamicsofiontransport
B.PassiveMediatedTransport
C.ActiveTransport

12/1

23

13

BiochemicalSignalingI
A.Hormones
B.ReceptorTyrosinekinases

12/3

24

13

BiochemicalSignalingII
A.HeterotrimericGProteins
B.PhosphoinositidePathway

12/4
ExamIV

Recitation14ReviewforExamIV
TuesdayDecember155:00PMto8:00PMSEC