Escolar Documentos
Profissional Documentos
Cultura Documentos
disease
N. S. Patel,* M. R. Peterson, D. A. Brenner, E. Heba, C. Sirlin, and R. Loomba
Author information Copyright and License information
The publisher's final edited version of this article is available at Aliment Pharmacol
Ther
See other articles in PMC that cite the published article.
Go to:
SUMMARY
Background
Ectopic fat deposition in the pancreas and its association with hepatic steatosis
have not previously been examined in patients with biopsy-proven non-alcoholic
fatty liver disease (NAFLD).
Aim
Methods
Results
The average MRI-determined pancreatic fat in patients with NAFLD was 8.5% and
did not vary significantly between head, body, and tail of the pancreas. MRIdetermined pancreatic fat content increased significantly with increasing histologydetermined hepatic steatosis grade; 4.6% in grade 1; 7.7% in grade 2; 13.0% in
grade 3 (P = 0.004) respectively. Pancreatic fat content was lower in patients with
histology-determined liver fibrosis than in those without fibrosis (11.2% in stage 0
fibrosis vs. 5.8% in stage 12 fibrosis, and 6.9% in stage 34 fibrosis, P = 0.013).
Pancreatic fat did not correlate with age, body mass index or diabetes status.
Conclusions
Go to:
INTRODUCTION
Non-alcoholic fatty liver disease (NAFLD) affects approximately 2030% of the adult
population in the western world and is becoming increasingly prevalent
worldwide.1, 2 It is well established that obesity, insulin resistance and other
components of metabolic syndrome are risk factors for the development of
NAFLD.3, 4
Obesity and metabolic syndrome are also associated with ectopic fat deposition in
other organs including the pancreas. This ectopic fat deposition in the pancreas
may trigger lipotoxicity in the pancreas.5 In the liver, this process of ectopic fat
deposition in the setting of metabolic syndrome may lead to cytokine-mediated
inflammation, lipotoxicity and oxidative stress resulting in hepatocellular injury,
inflammation and steatosis. This results in progressive liver disease termed as nonalcoholic steatohepatitis (NASH).6, 7 NASH may lead to cirrhosis, end-stage liver
disease and hepatocellular carcinoma, and is one of top three indications of liver
transplantation in the United States.810
In the pancreas, it has been suggested that fat accumulation, in the setting of
metabolic syndrome, may lead to a similar process that is termed as non-alcoholic
steatopancreatitis (NASP).11 Other clinical implications of pancreatic steatosis
include -cell dysfunction, exocrine dysfunction, increased risk of post-operative
fistula in patients undergoing pancreatic surgery, increased risk of dissemination
and mortality in co-existent pancreatic cancer and potentially greater severity of
episodes of acute pancreatitis.1217 Therefore, emerging data suggest that
pancreatic steatosis may have long-term clinical implications.
Recent studies have shown that pancreatic steatosis has a risk factor profile that is
similar to that seen in NAFLD including advanced age, obesity and insulin
resistance.1824 However, the association between novel magnetic resonance
imaging (MRI)-determined pancreatic fat content and histology-determined
steatosis grade in patients with biopsy-proven NAFLD has not been previously
studied. Previous studies have utilised imaging to assess pancreatic fat. In this
study, we utilised an advanced chemical shift-based gradient-echo MRI technique
that measures the proton-density-fat-fraction (PDFF), a standardised and
reproducible quantitative marker of fat content in tissue.25 Older MRI techniques
assessing steatosis are limited by T1 bias, T(2)* decay and multi-frequency signalinterference effects of protons in fat. This technique corrects for the above limiting
factors and provides a more accurate assessment of steatosis content using the
PDFF measurement.2629 MRI-PDFF of pancreas has not been specifically compared
with liver biopsy in adult patients with NAFLD. In this study, we aim to determine
whether MRI-PDFF of the pancreas is associated with liver histology-determined
steatosis and/or fibrosis in adults with NAFLD.
Go to:
METHODS
Study design and patient population
Inclusion criteria
Inclusion criteria included an age greater than 18 years, evidence of NAFLD on liver
biopsy as assessed by the NASH-CRN histological scoring system (please see Liver
histology assessment sub-section) and serum alanine aminotransferase (ALT) or
aspartate aminotransferase (AST) levels above the upper limit of normal (19 U/L or
more for women and 30 U/L or more for men).
Exclusion criteria
Clinical evaluation
After meeting inclusion and exclusion criteria, patients underwent a routine history
and physical examination in a research clinic. Body weight, height and vital sign
measurements were obtained and standard blood testing was performed, including
measurement of ALT, AST, alkaline phosphatase, gamma-glutamyl transpeptidase
(GGT), total bilirubin, direct bilirubin, albumin, fasting glucose and insulin,
haemoglobin A1c (HbA1c), lipid panel, free fatty acids (FFA) and C-reactive protein
(CRP). Homeostatic model of insulin resistance (HOMA-IR) was calculated as the
product of fasting insulin and glucose divided by a correction factor of 405.
All patients underwent liver biopsies within 6 months prior to inclusion in the study,
which were scored by a single liver pathologist (MP) using the NASH-CRN
histological scoring system.30 Biopsies were performed untargeted for the purpose
of evaluating for diffuse liver disease. Liver biopsy assessment included the
following variables: degree of steatosis (on a scale of 03), lobular inflammation (0
3), and hepatocellular ballooning (02). The sum of steatosis, lobular inflammation
and hepatocellular ballooning scores were added to determine the NAFLD activity
score (NAS) that ranges from 0 to 8. The liver fibrosis was staged from 0 to 4. The
pathologist was blinded to the clinical as well as the radiological data. As noted in
the inclusion criteria, patients were included in the study with a diagnosis of NAFLD
based on a liver steatosis grade of 1 or greater. All patients also had histological
evidence of either lobular inflammation or hepatocellular ballooning.
MRI protocol
This technique was used to measure liver as well as pancreatic fat in this study.
Images were obtained with a slice thickness of 8 mm without interslice gaps. To
measure MRI-determined liver steatosis, 3 regions of interest (ROIs) 300400 mm2
in area were placed in each of the nine liver segments on the PDFF parametric map.
This technique has been described in detail previously in patients with NAFLD and
has been shown to correlate well with histology-determined steatosis.36, 37
To measure pancreatic fat, 12 ROIs of 100 mm2 in area were placed in the head,
body and tail of the pancreas in each slice of the PDFF parametric map, with each
ROI at least 10 mm apart as shown in Figure 1. To minimise contamination from
volume averaging with extra-pancreatic adipose tissue, we placed ROIs in the head,
body and tail of the pancreas, making sure that the ROIs were surrounded by
pancreatic tissue not only within the imaging plane, but also on the slice above and
slice below. The head of the pancreas was defined as the area of the pancreas to
the anatomic right of the superior mesenteric vein. The body was defined as the
anatomic right half of the remaining pancreatic tissue and the tail was defined as
the anatomic left half of the remaining pancreatic tissue. The mean of all ROIs in
each part of the pancreas was calculated to determine the average fat fraction in
the head, body and tail, respectively, while the mean of all ROIs in the entire
pancreas determined the overall pancreatic fat fraction.
Figure 1
Figure 1
Measurement of pancreatic fat using MRI PDFF. A single source image of a magnetic
resonance image (MRI) gradient echo sequence of the abdomen is shown. Source
image was obtained with a slice thickness of 8 mm. Regions of interest (ROIs) 100
mm2 in area ...
A single resident physician who was trained in this method of MRI analysis
performed the measurements. The physician was blinded to clinical and histological
data and was under the supervision of the radiology investigator (CS). These
findings were cross-validated by an independent radiology investigator who was
blinded to the prior pancreatic fat fraction maps.
We hypothesised that pancreatic fat would positively correlate with histologydetermined steatosis grade in the liver. We would need a sample size of at least 40
to have an alpha of 0.05 with a power of 80% (or higher) requiring an effect size of
0.38 or higher.
Statistical analysis
The two-tailed t-test was used for comparison of continuous variables across
groups, while the Chi-squared test was used for comparisons of categorical
variables. Patients were stratified according to steatosis grade on liver biopsy and
the mean and standard error values were calculated for demographic, biochemical,
histological and MRI PDFF results. Statistical analyses with t-tests were performed
between the grade 1 and grade 3 steatosis groups. Paired t-tests were used to
compare MRI PDFF across different regions of the pancreas. All statistical analyses
were performed using Excel and SPSS software packages. A P-value <0.05 was
considered statistically significant.
Go to:
RESULTS
Demographic and biochemical data by liver steatosis grade
of age and included 24 men and 19 women. There was no statistically significant
difference in age and body mass index (BMI) across steatosis grades. Patients with
grade 1 steatosis were significantly more likely to have diabetes than those with
grade 3 steatosis (60.0% vs. 25.0%, P = 0.016). There was no statistically
significant difference across steatosis grades in biochemical data, including AST,
ALT, glucose, insulin, triglycerides, total cholesterol, low-density lipoprotein (LDL),
high-density lipoprotein (HDL), FFA, CRP, Hgb A1c, alkaline phosphatase, total
bilirubin, direct bilirubin and HOMA-IR. Pancreatic fat measured by MRI PDFF did not
significantly correlate with BMI (R2 = 0.019) or age (R2 = 0.015) in this cohort of
patients with NAFLD. In addition, there was no significant difference in average MRIdetermined pancreatic fat fraction between patients with diabetes (7.9%, n = 15)
and those without (8.8%, n = 28) in this population of patients with biopsy-proven
NAFLD.
Table 1
Table 1
Demographic and biochemical characteristics of patients with NAFLD by steatosis
grade
MRI-determined pancreatic fat across regions of the pancreas
The mean (standard error) MRI-determined pancreatic fat was 8.5 (1.0)%. The
mean pancreatic fat content did not vary significantly between the head, body and
tail of the pancreas, as shown in Figure 2.
Figure 2
Figure 2
MRI-determined pancreatic fat across regions of the pancreas. Mean magnetic
resonance image (MRI) proton density fat-fraction (PDFF) is shown for the head,
body and tail of the pancreas. Overall mean MRI PDFF was calculated as the mean
of all regions ...
Association between MRI-determined pancreatic fat and histology-determined liver
steatosis grade
Figure 3
Figure 3
MRI-determined pancreatic and liver fat across histology-determined steatosis
grade. Mean pancreas and liver fat percentage measured by magnetic resonance
image (MRI) proton density fat-fraction (PDFF) are shown according to steatosis
grade. Grade 1, ...
Patients with a higher histology-determined NAFLD activity score (NAS) also had
significantly higher pancreatic fat content. The mean MRI-determined pancreatic fat
content for subjects with an NAS <5 points was 6.4% (n = 22), while it was 10.6%
(n = 21) for those with an NAS 5 points (P = 0.03).
Figure 4
Figure 4
MRI-determined pancreatic fat across histology-determined fibrosis grade. Mean
pancreas fat percentage measured by magnetic resonance image (MRI) proton
density fat-fraction (PDFF) is shown according to fibrosis stage grouped by no
fibrosis (stage 0), ...
Go to:
DISCUSSION
Main findings
In this pilot study, utilising a novel MRI-technique that allows the non-invasive
quantification of pancreatic fat in a cohort of well-characterised patients with
biopsy-proven NAFLD, we demonstrate that pancreatic steatosis is common in
patients with NAFLD, and pancreatic fat content positively correlates with liver
histology-determined steatosis grade. Furthermore, the MRI-determined pancreatic
fat content is higher in patients who had increased NAFLD activity score (NAS 5)
on liver histology. Finally, we also found that pancreatic fat content is lower in
NAFLD patients who had advanced fibrosis. In summary, these findings suggest that
No correlation with BMI, age or diabetes was noted in our cohort of patients with
NAFLD, which differs from findings in prior studies. This may be explained by the
fact that prior studies focused on healthy patients without known liver disease. In
contrast, all patients in this study had NAFLD, 93.0% were overweight (BMI >24.9
kg/m2) and 67.1% were obese (BMI >29.9 kg/m2). Although only 14 of 43 patients
had diabetes, 64.3% of the remaining patients were pre-diabetic (A1c >5.7). In
addition, the role of diabetes is unclear, as Saisho et al. reported no association
between pancreatic fat and diabetes in a postmortem analysis of 1886 adults.20
One of the concerns with measuring pancreatic fat is the need for non-invasive
testing as a biopsy cannot be performed on living subjects to evaluate pancreatic
fat, inflammation and fibrosis. Some prior studies have relied on postmortem
histological analysis of the pancreas1820, 40, 41; however, inflammatory changes
with death can make this analysis unreliable as noted previously. Ultrasonography
has also been used, but provides a relatively insensitive measure of fat content.24,
42, 43 More recently, various MRI and MRS techniques have been used to measure
pancreatic fat.12, 13, 23, 38 We chose to use a novel chemical shift-based gradientecho MRI technique to measure PDFF because of its improved accuracy over
traditional techniques and because it has been validated in measuring fat content
non-invasively in human tissue.28, 34, 35, 44 A second concern is the uneven
accumulation of fat in the pancreas, which differs from the relatively homogenous
steatosis of the liver in NAFLD. Focal accumulation of fat in the pancreas,
particularly in the tail and anterior aspect of the head, has previously been
described using ultrasonography, computed tomography (CT) and MRI
techniques.42, 4548 Li et al. used a similar MRI technique as was used in our study
to measure fat content in the head, body and tail of the pancreas in healthy
subjects and noted no significant different in fat content across regions.23 Our
results are consistent with Li and colleagues and showed that there was no
significant difference in fat content between the head, body and tail of the
pancreas.
In our study, patients with histology-determined liver fibrosis had significantly less
pancreatic fat than those without evidence of liver fibrosis. It is possible that
pancreatic steatosis may have a similar mechanism of causing fibrosis in the
pancreas as the development of liver fibrosis in patients with NASH. Therefore, the
reduced degree of pancreatic steatosis in these patients may be related to
increased pancreatic fibrosis. Although the concept of pancreatic fibrosis in nonalcoholics has not been studied extensively, Pitchumoni et al. noted that fibrosis
was present in 29% of nonalcoholics in a postmortem analysis.49 Our study did not
use histology or imaging techniques to evaluate fibrosis of the pancreas; however, it
has been established previously that lower liver steatosis is associated with greater
liver fibrosis in patients with NAFLD.37 In addition, obesity and pancreatic steatosis
have been shown to result in increased cytokine production and fibrosis in the
pancreas in studies in which mice were fed a high fat diet.50, 51
The major strengths of this study include the use of an MRI technique that has been
well validated to measure fat content in the liver, histological assessment of the
liver, a patient population exclusively comprised of subjects with biopsy-proven
NAFLD and detailed biochemical and demographic data. As mentioned previously,
no prior studies have reviewed pancreatic fat in patients with biopsy-proven NAFLD.
In addition, measuring pancreatic fat in all anatomic areas of the pancreas allowed
for detailed measurements and confirmation of the homogenous nature of fat
distribution in the pancreas. Both the pathologist and radiologist were blinded to the
clinical data, and radiology or pathology data respectively. However, we
acknowledge following limitations of the study. We did not have a control and it is
neither feasible nor ethical to obtain a biopsy of the pancreas to confirm pancreatic
steatosis, and evaluate for changes in co-existent pancreatic fibrosis. In addition, we
do not have longitudinal data to help clarify whether pancreatic steatosis affects the
progression of NAFLD. We also acknowledge that an MRI slice thickness of 8 mm
may not provide optimal spatial resolution for measurement of fat in the pancreas.
We adopted the spectral model of fat derived from human liver in vivo by Hamilton
et al.52 While the spectral model of fat in human pancreatic tissue is likely to be
similar to that in liver tissue, this has not yet been experimentally verified. A
refinement for future studies will be the integration, if possible, of a spectral model
of fat derived from human pancreas in vivo. Finally, there was a small time interval
on average of 42.9 days between liver biopsy and MRI assessment in this study,
which could theoretically allow for a change in patient behaviour or management
before both studies were completed.
Go to:
CONCLUSIONS
MRI-determined pancreatic fat correlates with histology-determined liver steatosis
grade in patients with NAFLD. Pancreatic steatosis appears relatively homogenous
in patients with NAFLD. Further studies are needed to examine whether the
systemic effects and clinical consequences associated with ectopic fat deposition in
various organs, including the liver and pancreas, lead to either one common end
point such as cardiovascular disease or lead to end organ damage in each of these
organs independent of each other. Future studies are also needed to determine
whether pancreatic fat increases the risk of incident pancreatic exocrine or
endocrine insufficiency, or progressive pancreatic fibrosis.
Go to:
Supplementary Material
Supplemnetary figure
Figure S1. MRI-determined pancreatic fat across regions of the pancreas. Individual
subject and mean magnetic resonance image (MRI) proton density fat-fraction
(PDFF) is shown for the head, body and tail of the pancreas. Overall mean MRI PDFF
was calculated as the mean of all regions of interest (ROIs) in the pancreas. Head,
body and tail definitions are described in detail in the methods section. Paired twotailed t-test showed no statistical difference in MRI PDFF between regions of the
pancreas.
Go to:
Footnotes
Guarantor of the article: Rohit Loomba.
Author contributions: Niraj Patel was involved in analysis and interpretation of data,
statistical analysis, drafting of the manuscript and critical revision of the
manuscript. Michael Peterson, David A. Brenner and Claude Sirlin were involved in
critical revision of the manuscript. Elhamy Heba was involved in analysis of data
and critical revision of the manuscript. Rohit Loomba was involved in the study
concept and design, analysis and interpretation of data, drafting of the manuscript,
critical revision of the manuscript, obtained funding, study supervision. All authors
approved the final version of the manuscript.
SUPPORTING INFORMATION:
Additional Supporting Information may be found in the online version of this article:
Go to:
References
1. Bellentani S, Scaglioni F, Marino M, et al. Epidemiology of non-alcoholic fatty liver
disease. Dig Dis. 2010;28:15561. [PubMed]
2. Vernon G, Baranova A, Younossi ZM. Systematic review: the epidemiology and
natural history of non-alcoholic fatty liver disease and non-alcoholic steatohepatitis
in adults. Aliment Pharmacol Ther. 2011;34:27485. [PubMed]
3. Clark JM. The epidemiology of nonalcoholic fatty liver disease in adults. J Clin
Gastroenterol. 2006;40(Suppl. 1):S510. [PubMed]
4. Kotronen A, Peltonen M, Hakkarainen A, et al. Prediction of non-alcoholic fatty
liver disease and liver fat using metabolic and genetic factors. Gastroenterology.
2009;137:86572. [PubMed]
5. van Raalte DH, van der Zijl NJ, Diamant M. Pancreatic steatosis in humans: cause
or marker of lipotoxicity? Curr Opin Clin Nutr Metab Care. 2010;13:47885.
[PubMed]
6. Brunt EM, Janney CG, Di Bisceglie AM, et al. Nonalcoholic steatohepatitis: a
proposal for grading and staging the histological lesions. Am J Gastroenterol.
1999;94:246774. [PubMed]
7. Diehl AM, Li ZP, Lin HZ, et al. Cytokines and the pathogenesis of nonalcoholic
steatohepatitis. Gut. 2005;54:3036. [PMC free article] [PubMed]
8. Farrell GC, Larter CZ. Nonalcoholic fatty liver disease: from steatosis to cirrhosis.
Hepatology. 2006;43:S99112. [PubMed]
9. Baffy G, Brunt EM, Caldwell SH. Hepatocellular carcinoma in nonalcoholic fatty
liver disease: an emerging menace. J Hepatol. 2012;56:138491. [PubMed]
10. Torres DM, Williams CD, Harrison SA. Features, diagnosis, and treatment of
nonalcoholic Fatty liver disease. Clin Gastroenterol Hepatol. 2012;10:83758.
[PubMed]
11. Pitt HA. Hepato-pancreato-biliary fat: the good, the bad and the ugly. HPB
(Oxford) 2007;9:927. [PMC free article] [PubMed]
12. Tushuizen ME, Bunck MC, Pouwels PJ, et al. Pancreatic fat content and beta-cell
function in men with and without type 2 diabetes. Diabetes Care. 2007;30:291621.
[PubMed]
13. Heni M, Machann J, Staiger H, et al. Pancreatic fat is negatively associated with
insulin secretion in individuals with impaired fasting glucose and/or impaired
glucose tolerance: a nuclear magnetic resonance study. Diabetes Metab Res Rev.
2010;26:2005. [PubMed]
14. Lozano M, Navarro S, Perez-Ayuso R, et al. Lipomatosis of the pancreas: an
unusual cause of massive steatorrhea. Pancreas. 1988;3:5802. [PubMed]
15. Mathur A, Pitt HA, Marine M, et al. Fatty pancreas: a factor in postoperative
pancreatic fistula. Ann Surg. 2007;246:105864. [PubMed]
16. Mathur A, Zyromski NJ, Pitt HA, et al. Pancreatic steatosis promotes
dissemination and lethality of pancreatic cancer. J Am Coll Surg. 2009;208:98994.
[PubMed]
17. Navina S, Acharya C, DeLany JP, et al. Lipotoxicity causes multisystem organ
failure and exacerbates acute pancreatitis in obesity. Sci Transl Med. 2011;3:10710.
[PMC free article] [PubMed]
18. Olsen TS. Lipomatosis of the pancreas in autopsy material and its relation to age
and overweight. Acta Pathol Microbiol Scand A. 1978;86A:36773. [PubMed]
19. Stamm BH. Incidence and diagnostic significance of minor pathologic changes in
the adult pancreas at autopsy: a systematic study of 112 autopsies in patients
without known pancreatic disease. Hum Pathol. 1984;15:67783. [PubMed]
20. Saisho Y, Butler AE, Meier JJ, et al. Pancreas volumes in humans from birth to
age one hundred taking into account sex, obesity, and presence of type-2 diabetes.
Clin Anat. 2007;20:93342. [PMC free article] [PubMed]
21. Lee JS, Kim SH, Jun DW, et al. Clinical implications of fatty pancreas: correlations
between fatty pancreas and metabolic syndrome. World J Gastroenterol.
2009;15:186975. [PMC free article] [PubMed]
22. Sijens PE, Edens MA, Bakker SJ, et al. MRI-determined fat content of human liver,
pancreas and kidney. World J Gastroenterol. 2010;16:19938. [PMC free article]
[PubMed]
23. Li J, Xie Y, Yuan F, et al. Noninvasive quantification of pancreatic fat in healthy
male population using chemical shift magnetic resonance imaging: effect of aging
on pancreatic fat content. Pancreas. 2011;40:2959. [PubMed]
24. Sepe PS, Ohri A, Sanaka S, et al. A prospective evaluation of fatty pancreas by
using EUS. Gastrointest Endosc. 2011;73:98793. [PubMed]
25. Bydder M, Yokoo T, Hamilton G, et al. Relaxation effects in the quantification of
fat using gradient echo imaging. Magn Reson Imaging. 2008;26:34759. [PMC free
article] [PubMed]
26. Liu CY, McKenzie CA, Yu H, et al. Fat quantification with IDEAL gradient echo
imaging: correction of bias from T (1) and noise. Magn Reson Med. 2007;58:35464.
[PubMed]
27. Yu H, McKenzie CA, Shimakawa A, et al. Multiecho reconstruction for
simultaneous water-fat decomposition and T2* estimation. J Magn Reson Imaging.
2007;26:115361. [PubMed]
28. Hines CD, Frydrychowicz A, Hamilton G, et al. T(1) independent, T(2) (*)
corrected chemical shift based fat-water separation with multi-peak fat spectral
modeling is an accurate and precise measure of hepatic steatosis. J Magn Reson
Imaging. 2011;33:87381. [PMC free article] [PubMed]
29. Yu H, Shimakawa A, McKenzie CA, et al. Multiecho water-fat separation and
simultaneous R2* estimation with multifrequency fat spectrum modeling. Magn
Reson Med. 2008;60:112234. [PMC free article] [PubMed]
30. Kleiner DE, Brunt EM, Van Natta M, et al. Design and validation of a histological
scoring system for nonalcoholic fatty liver disease. Hepatology. 2005;41:131321.
[PubMed]
31. Yu H, Shimakawa A, Hines CD, et al. Combination of complex-based and
magnitude-based multiecho water-fat separation for accurate quantification of fatfraction. Magn Reson Med. 2011;66:199206. [PMC free article] [PubMed]
32. Hernando D, Hines CD, Yu H, et al. Addressing phase errors in fat-water imaging
using a mixed magnitude/complex fitting method. Magn Reson Med. 2012;67:638
44. [PMC free article] [PubMed]
33. Reeder SB, Cruite I, Hamilton G, Sirlin CB. Quantitative assessment of liver fat
with magnetic resonance imaging and spectroscopy. J Magn Reson Imaging.
2011;34:72949. [PubMed]
34. Kang GH, Cruite I, Shiehmorteza M, et al. Reproducibility of MRI-determined
proton density fat fraction across two different MR scanner platforms. J Magn Reson
Imaging. 2011;34:92834. [PubMed]
35. Schwenzer NF, Machann J, Martirosian P, et al. Quantification of pancreatic
lipomatosis and liver steatosis by MRI: comparison of in/opposed-phase and
spectral-spatial excitation techniques. Invest Radiol. 2008;43:3307. [PubMed]
36. Le TA, Chen J, Changchien C, et al. Effect of colesevelam on liver fat quantified
by magnetic resonance in nonalcoholic steatohepatitis: a randomized controlled
trial. Hepatology. 2012;56:92232. [PMC free article] [PubMed]
37. Permutt Z, Le TA, Peterson MR, et al. Correlation between liver histology and
novel magnetic resonance imaging in adult patients with non-alcoholic fatty liver
disease -MRI accurately quantifies hepatic steatosis in NAFLD. Aliment Pharmacol
Ther. 2012;36:229. [PMC free article] [PubMed]
38. Rossi AP, Fantin F, Zamboni GA, et al. Predictors of ectopic fat accumulation in
liver and pancreas in obese men and women. Obesity (Silver Spring) 2011;19:1747
54. [PubMed]
39. Hannukainen JC, Borra R, Linderborg K, et al. Liver and pancreatic fat content
and metabolism in healthy monozygotic twins with discordant physical activity. J
Hepatol. 2011;54:54552. [PubMed]
40. van Geenen EJ, Smits MM, Schreuder TC, et al. Nonalcoholic fatty liver disease is
related to nonalcoholic fatty pancreas disease. Pancreas. 2010;39:118590.
[PubMed]
41. Orci L, Stefan Y, Malaisse-Lagae F, et al. Pancreatic fat. N Engl J Med.
1979;301:1292. [PubMed]
42. Marchal G, Verbeken E, Van Steenbergen W, et al. Uneven lipomatosis: a pitfall
in pancreatic sonography. Gastrointest Radiol. 1989;14:2337. [PubMed]
43. Glaser J, Stienecker K. Pancreas and aging: a study using ultrasonography.
Gerontology. 2000;46:936. [PubMed]
44. Meisamy S, Hines CD, Hamilton G, et al. Quantification of hepatic steatosis with
T1-independent, T2-corrected MR imaging with spectral modeling of fat: blinded
comparison with MR spectroscopy. Radiology. 2011;258:76775. [PMC free article]
[PubMed]
45. Matsumoto S, Mori H, Miyake H, et al. Uneven fatty replacement of the
pancreas: evaluation with CT. Radiology. 1995;194:4538. [PubMed]
46. Isserow JA, Siegelman ES, Mammone J. Focal fatty infiltration of the pancreas:
MR characterization with chemical shift imaging. AJR Am J Roentgenol.
1999;173:12635. [PubMed]
47. Kim HJ, Byun JH, Park SH, et al. Focal fatty replacement of the pancreas:
usefulness of chemical shift MRI. AJR Am J Roentgenol. 2007;188:42932. [PubMed]
48. Kawamoto S, Siegelman SS, Bluemke DA, et al. Focal fatty infiltration in the
head of the pancreas: evaluation with multidetector computed tomography with
multiplanar reformation imaging. J Comput Assist Tomogr. 2009;33:905. [PubMed]
49. Pitchumoni CS, Glasser M, Saran RM, et al. Pancreatic fibrosis in chronic
alcoholics and nonalcoholics without clinical pancreatitis. Am J Gastroenterol.
1984;79:3828. [PubMed]
50. Mathur A, Marine M, Lu D, et al. Nonalcoholic fatty pancreas disease. HPB
(Oxford) 2007;9:3128. [PMC free article] [PubMed]
51. Zhang X, Cui Y, Fang L, et al. Chronic high-fat diets induce oxide injuries and
fibrogenesis of pancreatic cells in rats. Pancreas. 2008;37:e318. [PubMed]
52. Hamilton G, Yokoo T, Bydder M, et al. In vivo characterization of the liver fat (1)H
MR spectrum. NMR Biomed. 2011;24:78490. [PMC free article] [PubMed]
53. Fischer MA, Nanz D, Reiner CS, et al. Diagnostic performance and accuracy of 3D spoiled gradient-dual-echo MRI with water- and fat-signal separation in liver-fat
quantification: comparison to liver biopsy. Invest Radiol. 2010;45:46570. [PubMed]