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The 6th National Biotechnology Congress of Iran

13-15 Aug, 2009, Milad Tower Conference Hall, Tehran-Iran

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1_ Open Reading Frame

1_ Double antibody sandwich
2_ Reverse Transcription
3_ Polymerase Chain Reaction

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The 6th National Biotechnology Congress of Iran

13-15 Aug, 2009, Milad Tower Conference Hall, Tehran-Iran

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The 6th National Biotechnology Congress of Iran

13-15 Aug, 2009, Milad Tower Conference Hall, Tehran-Iran

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CH-SqMV
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YAZ-YAZ

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Refrences:
1-Carette, J.E., Verver, J., Martens, J., Vankampen, T., Wellink, J. and Vankammen, A. 2002. Characterisation
of plant proteins that interact with cowpea mosaic virus 60kd protein in the yeast two-hybrid system, Journal of
General Virology 83:885-893.
2- DNAMAN version 4. 02 lynnon Biosoft. 1994-98.
3-Han, S. S., Yoshida, K., Karasev, A. V. and Iwanami, T. 2002. Nucleotide sequence of a Japanese isolate of
Squash mosaic virus. Archives of Virology 147: 437-443
4-Kalendar, R. 2005. Fast PCR primer design and repeat sequence searching program with additional tools for
the manipulation and analysis of DNA and protein. http://www. Biocenter.helsinki.fi/bi/programes/fastpcr.htm.
5- Lomonossof, G.P. and Shanks, M. 1983. The nucleotide sequence of Cowpea mosaic virus B-RNA .The EMBO
journal, Vol.2 No.6 pp: 941-946.
6- Pouwels, J., curette, J, E, Vanlent, J. and wellink, J.2002. Cowpea mosaic virus: effects on host cell processes.
Mollecular Plant Pathology, 3(6):411-418.
7- URL: http://ncbi.nlm.nih.gov/Genebank/

Characterization of the B-RNA segment of Iranian Squash mosaic

virus isolates
Shamshiri, N ., Massumi, H

., Heydarnejad, J., Hosseini pour, A and Shaabanian, M.

Department of plant protection, College of agriculture, Shahid Bahonar University of Kerman, Iran
Najmehshamshiri@yahoo.com

Abstract
Squash mosaic virus (SqMV) is one of the cucurbit infecting viruses worldwide. The genome of the
virus consists of two segment of linear positive sense ssRNA refer to B-RNA and M-RNA
(RNA1 and RNA2, respectively). The B-RNA comprises five open reading frames that encode
Protease cofactor, Helicase, Vpg, Protease and RNA dependent RNA polymerase (RdRp). In this
study the B-RNA segment of two Iranian isolates of SqMV collected from Yazd and Khorasan
Razavi (Taibad region) were amplified by RT-PCR using specific primers. Amplified products
were cloned and sequenced. The full-length B-RNA segment of both isolates comprised 5757
nucleotides, with five open reading frames. Sequence comparison showed 97.9% nucleotide
sequence identity between Iranian isolates. When Iranian and GenBank isolates were compared,

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The 6th National Biotechnology Congress of Iran

13-15 Aug, 2009, Milad Tower Conference Hall, Tehran-Iran

the maximum identity was 88,5% between Taibad and Japanese isolates. Based on limited
prevalence of SqMV in Iran it can be concluded that this virus possibly comprise a low genetic
variation in Iran.