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RATING:
I.INTRODUCTION
A.
II. METHODOLOGY
SET-UP 1: Hydrila Plant
In this experiment, our professor, Sir Roque, assigned different
percentage of solution for each groups and well just share the data we have gathered.
Our group was assigned to use 5% sodium chloride solution. For this experiment, we
used only one (1) leaf of hyrdrilla. We placed the leaf on the slide and put 2 drops of 5%
solution on it and set aside for five (5) minutes. While waiting for the 5 minutes, we
focused the microscope, at first, it was very difficult to find light but eventually we
succeeded. After 5 minutes, we mounted the leaf poured with the solution, put it in the
microscope and observed what happened to the cells. After all our classmates have
seen the specimen, we were asked to pour two (2) drops of distilled water on the
specimen and again, observe what will happen to the cells.
In
these
various
Salt
Solutio
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In the
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uent
distille
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water
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Set-up 2
In this set up, it shows the Appearance of Elodea Cells in various salt solutions.
At 0% solution the cell wall is still normal and visible as the concentration of salt
increases spaces are observe because water inside the cytoplasm moves out and the
organelles are moving closer to each other. When distilled water are added the cell
starts to go back to its normal view in the microscope however if the concentration
reach 5% elodea cell reach the threshold where it no longer reverse the process.
In what % NaCl have you seen a most significant change in the Elodea cell?
Why is this so?
Elodea leaf at 5% of salt solution. Elodea is a fresh water plant and it
plasmolyzed significantly in this solution. Plasmolysis would occur because the high
concentration of salt outside the cells would cause the diffusion of water from within the
cell to outside the cells.
If normal Elodea plant cell is put directly into distilled water
a. in which direction is the movement of water?
Both direction inside and outside the cell.
b. Is isotonicity ever attained?Explain clearly and fully why or why not?
Yes because the inside and outside the cell has the same solute
concentration as another solution, the cytoplasm in this case, will not gain or lose water
because water molecules will enter and leave the cell at equal rates.
A red blood cell is basically a bag of hemoglobin. Water but not
hemoglobin can flow freely across the membrane. If a red blood cell were placed
into distilled water, what would happen and what would be the ultimate outcome
due to the process of osmosis?
If a red blood cell were placed into distilled water to blood on a microscope slide,
the cells will look bigger after a few seconds. These are the two explanations:
Molecules such as protein and polysaccharides are more concentrated inside the
cell than outside the cell when the cell is in distilled water. These molecules therefore
begin to move out of the cell because of the process of diffusion but are blocked by the
cell membrane. As a result, these molecules push on the cell membrane and make the
cell appear bigger.
Water molecules move into the cell because the concentration of water is greater
outside the cell than it is inside the cell. As a result, water fills the cell and makes it
appear bigger
Were you able to detect threshold of dehydration in elodea cells beyond
the cells can no longer reverse the process? If so what was it?
Observation of plasmolysis when the leaf is flooded with 5%salt water.
Cell death thresholds were determined for three cell populations in the leaves for
each frequency. The intensity thresholds were strongly dependent on frequency, and
the minimum threshold for each population generally occurred near the expected
resonance frequency of the gas bodies associated with each population.