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Article history:
Received 10 June 2008
Received in revised form
14 September 2009
Accepted 6 November 2009
This study was undertaken to evaluate the functional properties of two of the most popular species of
edible bamboo shoots in Korea (Phyllostachys pubescens and Phyllostachys nigra). Powdered bamboo
shoots were extracted with methanol and an aqueous suspension of the obtained methanol extract was
partitioned successively with chloroform, ethyl acetate, and butanol, leaving a residual water extract. All
obtained extracts were evaluated for their antioxidant capacity and antimicrobial activity, angiotensin
converting enzyme (ACE) inhibition activity, and ascorbic acid and phenolic compound content. Methanol and water fractions showed a particularly high ascorbic acid contents. The ethyl acetate fraction
contained a high concentration of phenolic compounds. Among all extracts, the ethyl acetate and butanol
fractions showed particularly high antioxidant activity. Methanol extract had a signicantly higher ACE
inhibitory activity than other extracts. None of the extracts inhibited the tested bacteria.
2009 Elsevier Ltd. All rights reserved.
Keywords:
Bamboo shoots
Antioxidant
Antimicrobial
ACE inhibition
Phenolic compound
1. Introduction
Bamboo is a group of genera of evergreen plants belonging to the
Poaceae or grass family. Bamboo shoots are the immature and edible
culms arising from the rhizomes. Shoots emerge in early spring, can
grow quickly at over 1 m per day, and usually become lignied
(woody) in 23 days (Luo, Xi, Fu, & Lu, 2002; Wang, 2002; Zhang,
Yang, Han, & Dong, 2000). For these reasons, bamboo shoots have
limitations for consumption and storage. Among the edible 6090
genera of bamboo shoots, Phyllostachys pubescens and Phyllostachys
nigra are the major species cultivated in Korea (statistical data of
Korea Forest Research Institute, 2005). Bamboo leaves (Phyllostachys
Sieb. et Zucc.) have antioxidant capacity due to high polyphenol
content (Lu, Wu, Shi, Dong, & Zhang, 2006). Park, Lim, Kim, Choi, and
Lee (2007) reported that a butanol extract of bamboo leaves (Sasa
borealis) exhibited signicant antioxidant capacity against the
1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Ethanol extracts of
bamboo (Phyllostachys bambusoides) have a nitrite scavenging
ability (Lim, Na, & Baik, 2004). Kim, Cho, Lee, Ryu, and Shim (2001)
reported that extracts of bamboo leaves and stems (Phyllostachys
spp.) showed strong antibacterial activities. Most research studies
656
E.-J. Park, D.-Y. Jhon / LWT - Food Science and Technology 43 (2010) 655659
E.-J. Park, D.-Y. Jhon / LWT - Food Science and Technology 43 (2010) 655659
Table 1
The extraction yields of powdered bamboo shoots by various solvents.
Solvent
Methanol extract
Chloroform fraction
Ethyl acetate fraction
Butanol fraction
Water fraction
30.3
4.2
0.7
8.4
15.4
2.1b
0.3
0.0
1.1
1.0
P. nigra (PN)
24.3
5.6
1.0
5.5
12.1
657
1.8
0.2
0.1
0.8
0.9
a
Yield ratios (g/100 g, w/w) [solid in extract or fraction (g)/raw material (100 g,
dry weight)].
b
All experiments were replicated three times and results are presented as mean SE.
Table 2
Ascorbic acid and phenolic compounds in bamboo shoot (Phyllostachys pubescens, PP) extracts by various solvents.
Compound
CHCl3
EtOAc
BuOH
H2O
Ascorbic acid
Protocatechuic acid
p-Hydroxybenzoic acid
Catechin
Caffeic acid
Chlorogenic acid
Syringic acid
p-Coumaric acid
Ferulic acid
154.7 8.5a
2.8 0.2
1.7 0.0
0
0
0
0
0
0
1.0 0.0
0.1 0.0
0.4 0.0
0
0
0
0
0
0
51.0 3.2
0.5 0.0
2.9 0.0
0
0.3 0.0
0
1.3 0.0
1.3 0.0
0.5 0.0
50.9 1.7
1.3 0.0
0.6 0.0
0
0
0
6.7 0.2
0
0
136.3 4.2
0
0
0
0
0
0
0
0
All experiments were replicated three times and results are presented as mean SE.
658
E.-J. Park, D.-Y. Jhon / LWT - Food Science and Technology 43 (2010) 655659
Table 3
Ascorbic acid and phenolic compounds in bamboo shoot (Phyllostachys nigra, PN) extracts by various solvents.
Compound
Ascorbic acid
Protocatechuic acid
p-Hydroxybenzoic acid
Catechin
Caffeic acid
Chlorogenic acid
Syringic acid
p-Coumaric acid
Ferulic acid
a
CHCl3
EtOAc
195.3 3.4a
0
5.4 0.2
0
0
0
0
0
0
9.3 0.8
0
0.6 0.0
0
0
0
1.8 0.0
0
0
162.5
1.5
8.1
10.6
1.5
4.1
2.5
3.5
1.8
5.7
0.0
1.2
1.9
0.0
0.3
0.0
0.1
0.0
BuOH
H2O
114.3 3.2
0
1.4 0.0
0
0
3.5 0.5
2.1 0.1
0
0
231.8 10.1
0
0
0
0
0
0
0
0
All experiments were replicated three times and results are presented as mean SE.
Table 4
Antioxidant capacity of solvent fractions from the powdered bamboo shoots and
control antioxidant by DPPH radical scavenging method.
Fraction
Methanol extract
Chloroform fraction
Ethyl acetate fraction
Butanol fraction
Water fraction
BHT
BHA
Ascorbic acid
Catechin
3.6
4.0
0.8
0.7
4.7
0.2b
0.0
0.0
0.0
0.3
0.006
0.008
0.009
0.023
0.0
0.0
0.0
0.0
P. nigra (PN)
3.4
2.3
0.4
0.8
5.3
0.3
0.0
0.0
0.0
0.8
0.006
0.008
0.009
0.023
0.0
0.0
0.0
0.0
a
Amount of bamboo shoots extract required for 50% reduction of free radical
scavenging activity.
b
All experiments were replicated three times and results are presented as
mean SE.
70
60
50
40
30
20
10
0
0
10
12
Concentration (mg/ml)
Fig. 1. Angiotensin converting enzyme (ACE) inhibition activity by solvent fractions
from powdered bamboo shoots (Phyllostachys pubescens, PP). C, methanol fraction; B
chloroform fraction; : ethyl acetate fraction; 6 butanol fraction; - water fraction.
E.-J. Park, D.-Y. Jhon / LWT - Food Science and Technology 43 (2010) 655659
80
70
60
50
40
30
20
10
0
0
10
12
Concentration (mg/ml)
Fig. 2. Angiotensin converting enzyme (ACE) inhibition activity by solvent fractions
from powdered bamboo shoots (Phyllostachys nigra, PN). C methanol fraction; B
chloroform fraction; : ethyl acetate fraction; 6 butanol fraction; - water fraction.
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