The GcMAF Book

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TheGcMAFBook
The GcMAF Book
tableofcontents
Table of Contents
Preface:GcMAFandNagalase:twoamazingproteins.
Introduction:RoutineNagalasetestingfindscancerearlyandGcMAFcuresit
Chapter1:ACureforMetastaticCancer?
Chapter2:ProfessorYamamotoandRealScience
Chapter3:YourIncredibleImmuneArmy
Chapter4:TheWaronCancerInsideUs
Chapter5:YourImmuneCellsVersusThePathogenArmy:ANanoscaleWar
Chapter6:YourAwesomeMacrophageKillingMachine
Chapter7:MacrophagesNeedGcMAFtoThrive
Chapter8:HowYourBodyMakesGcMAF
Chapter9:Nagalase:FriendandFoe?
Chapter10:HowNagalaseBlocksGcMAFProduction
Chapter11:IfCancerCellsCouldTalk...
Chapter12:GcMAFandHIV/AIDS
Chapter13:TheAMASTestAnAlternativeToNagalaseTesting
Chapter14:BiomarkerTesting
Chapter15:EradicatingtheScourgeofCancerfromtheFaceoftheEarth
Chapter16:RetroDocsandNanoMedicine:ARant
Chapter17:DifferentiationandCancerGrading
Chapter18:TheCancercontinuumandthe'Pointofnoreturn'
Chapter19:GcMAFTherapyGuidelines
Chapter20:WhyNotSkipConventionalCancerTherapiesandJustTakeGcMAF?
Chapter21:Knockoffs,Wannabes,Bootlegs,CounterfeitsandCertification
http://gcmaf.timsmithmd.com/book/book/4/
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Appendix1:AboutDr.NobutoYamamoto
Appendix2:TheYamamotoPapers
Appendix3:AbouttheAuthor
References
Copyright2010TimothyJ.Smith,M.D.
http://gcmaf.timsmithmd.com/book/book/4/
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Preface:GcMAFandNagalase:twoamazingproteins.
The GcMAF Book
tableofcontentsnext
Preface
GcMAF and Nagalase: two amazing proteins.

Inthepagesthatfollowyouaregoingtolearnabouttwoamazingproteins:GcMAF(glycoproteinmacrophage
activatingfactor)andNagalase(alphaNacetylgalactosaminidase).Thesetwonaturalbioidenticalprotein
molecules(madebyourbodiesusingourowngeneticprogram)havethepotentialtopreventalotofhuman
sufferingandsavemillionsoflives.
ResearchstudieshaveshownhowwecanuseGcMAFandNagalaseto:
identifythepresenceofandreversemetastaticcancer
cureHIVandotherchronicviralinfections
detectandreverseearlycancerslongbeforeimagingcanidentifythem
determinewhetheracancertreatmentprogramisworking
Nagalasescreening,coupledwithearlytreatmentusingGcMAF,hasthepotentialtoridtheworldofthescourge
ofcancer.Thisisastrongstatement,butanyonewhocarefullyexaminesthesciencebehindGcMAF,Nagalase,
andthemolecularbiologyofmacrophageactivationwillrealizeitistrue.
Manypeoplearegoingtowanttogettheirhandsonsomeofthisstuff.Unfortunately,certifiedpure
bioidenticalGcMAFisnotyetavailable.
Inwritingthisbook,Ihavehadtwoprimarygoals,bothofwhichareattainable.Thefirstistofurtherthecauseof
makingGcMAFavailabletoallwhoneedit.ThesecondistopromotetheestablishmentofNagalasecancer
screeningprogramsforalladultsandallhighriskpopulations.WithNagalasetestingwenowpossessthe
technologytoidentifycancerwhenitisjustahandfulofcellsandtheneasilyreverseitwithafewGcMAF
injections.Forme,theHolyGrailofcancereradicationisfindingitearlyandnippingitinthebud.
http://gcmaf.timsmithmd.com/book/chapter/42/
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previoustableofcontentsnext
Introduction
Routine Nagalase testing finds cancer early and GcMAF

cures it
IfwecanaccelerateresearchonNagalasetestingandGcMAFtreatment,thefollowingconversations
mighthappenfiveyearsfromnow:
JoehasanappointmentwithDr.Jones,hisfamilydoctorforthepasttwodecades,todiscussJoesannuallabtest
results.
HiJoe.Goodtoseeyou!saysDr.Jones.WeransomescreeningtestsonyourbloodandIvegotsomegood
newsandsomebadnews.
Itsbeenalongday,doc.Howaboutthegoodnewsfirst,ifyoudontmind,saysJoe.
Sure.Yourbloodteststellmeyouareapictureofhealth.Yourcholesterolandotherheartmarkersareall
normal.YourvitaminDlevelisexcellentat70.YourPSAislow.Yourthyroidisinbalance.Yourcompleteblood
countandothermetabolicparametersareperfect.
Soundsgood,doc,andthatallfitsperfectlywiththefactthatIfeelgreat!sowhatsthebadnews?
Well,Joe,yourNagalaseleveliselevated.andthatsgotmealittleconcerned.
MyNagalaseiselevated?Whatisthatandwhatdoesthatmean?saysJoecautiously.
NagalaseshortforalphaNacetylgalactosaminidaseisanenzymemadebyvirusesandcancercells.Wecan
testforit.Anelevatedleveltellsmeyouhavetheveryearliestbeginningsofeitheracanceroravirus.
Nagalasetestingisalotlikecholesteroltesting.Elevatedcholesterolisassociatedwithincreasedriskofarterial
plaqueandheartattack.AnelevatedNagalasetellsusyouareatincreasedriskofcancer.
Cancerorvirus?Idontunderstand.Whatdoesthatmean,doc?
BothcancersandvirusesmakeNagalase,andatthispointIcanttellyouwhichitis.Buttheresnothingto
worryabouthere,Joe.Youdonthaveanysymptoms,soitprobablyisntavirus.Wellgetsomeantibodyteststo
ruleoutthatpossibility,andwellrepeatyourNagalaseleveltoseewhetheritisgoingupornot.Fornow,allwe
needtodoiskeepaneyeoneverything.Iftheelevationiscausedbyavirus,thelevelwillgobackdown.Ifthe
elevatedNagalaseiscausedbyanearlycancer,theNagalasewillkeepgoingup,andwelltreatitandmakeitgo
away.Heresyourlaborder.Seeyouinamonth,okay?
ShouldIbeworried,doc?
No.
Imighthavecancer,butnottoworry?Howdoesthatwork?
Fiveyearsago,Joe,Idbeveryconcerned.ButbackthenwedidnthaveNagalasetestingfordetectingearly
cancers,soIwouldnthaveevenknownthatacancerwasgettingstartedinyou.Atleastnotuntilitgotalot
bigger.Andfiveyearsago,ifIdidhavesomewaytoknowyouhadearlycancer,Iwouldnthavehadawayto
reverseit.Now,Ihaveboth.AndImusttellyou,asadoctorwhohasseenalotofpeoplesufferanddiefrom
cancer,weareextremelyfortunatethatthesetoolsarenowavailable.Millionsofoliveshavealreadybeensaved
bycatchingcancersearlyandreversingthembeforetheygotoutofcontrol.So,nottoworry,Joe.Ifitscancer,
wehavethetoolstogetridofit.Seeyouinamonth.
Okay.
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Onemonthlater
Joe,yourviralantibodytestsallcamebacknormal,sonowweknowyoudonthaveaviralinfection.Your
Nagalase,however,continuestoclimbhigher.Thattellsmeyouhaveaverysmallearlystagecancergrowingin
yousomewhere.
Ivegotcancer?Yikes!Youdidtellmethiswasapossibility.NowshouldIbeworried?
No,Joe,youreallyhavenothingtoworryabout.Wehaveatreatmentthatwillmakethiscancergoaway.And
wecanaccomplishthatlongbeforeitgetsbigenoughtoshowuponaCATscan.
Doesitmatterwhatkindofcanceritis?
Nope.
Doesitmatterwhereitislocated?
Nope.
ShouldntIgetaCATscansowecanfindit?
SinceyourNagalaseisonlyslightlyelevated,Iknowthatyourcancersstillwaytoosmalltoseeonimaging.But
thecancercellsaredefinitelyinthere,makingtheNagalasethatweseeonyourbloodtest.
CouldnttherebesomethingelsethatsmakingtheNagalase?saysJoe.
No,Nagalaseisonlymadebycancersandviruses,andweveeliminatedthepossibilityofavirus,soithastobe
cancer.
Howsooncanwestarttreatingit?
Inonemonth.IwanttogetathirdNagalaselevel,justtomakesureandifitisstillgoingup,thenwecanbe
certainitiscancer,andwellstartthetreatment.
Anothermonthgoesby
Well,Joe,yourNagalaseisstillclimbing,sowecanstarttreatmenttoday.
Okay.Whatdowedo?Whatisthetreatment?
WeeklyinjectionsofGcMAF.WellkeeponmeasuringyourNagalaseonceamonth,andwhenitgetsbackdown
tonormal,wellknowthecancerhasbeencured,sothatswhenwellstopgivingyoutheshots.
Howlongdoesthattake?
Usuallythreetosixmonths.
WhatisGcMAF,anyway?
GcMAFgroupspecificcomponentmacrophageactivatingfactoristheproteinyourbodymakestoactivateyour
anticancerimmuneactivity
Anticancerimmuneactivity.Whatsthat,Dr.Jones?
Joe,yourimmunesystemcontainssomeverylargecellscalledmacrophagesthat,whenactivated,willtrack
down,attack,devour,andkillvirusesandcancercells.GcMAFanimmuneproteinmadebyyourlymphocytesis
whatliterallyturnsthemon.WithoutGcMAF,yourmacrophagesremaininastateofsuspendedanimationsort
oflikezombies.
YoumeanifIdonthaveenoughGcMAFtheystopkillingofftheinvaders?Thatcantbegood.
No,itisnt,Joe.Thisisnotasubtleslowdown,either.Intermsofkillingpower,anactivatedmacrophageisabout
40timesmoreaggressivethanadeactivatedmacrophage.WithoutGcMAF,theyjustgotosleep.
Wow.
YourcancercellshavefiguredoutthatiftheyblocktheproductionofGcMAF,theycandisabletheirarchenemies,
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themacrophages,andgettheupperhand.WithoutGcMAFtoactivatethem,themacrophagesdonthavea
chance.
So,doc,howdidtheydoit?Howdidmycancercellsdisablemymacrophages.
BymakingNagalase.
Thesamestuffyouvebeentesting?
Yup.NagalaseblocksGcMAFproduction,sowhenweseearisingNagalase,wepayverycloseattention.Itisan
incrediblysensitivetest,andittellsuscancerisonthemove.Ascancersgrow,theygeneratemoreandmore
Nagalase.
SotherisingNagalasenumberstoldyoumycancercellswerewinningthewar.
Yes.repliesDr.Jones.
AndthattheNagalasehadputmymacrophagestosleepbyblockingmybodysGcMAFproduction.
Right.Theremaybeafutureforyouinmolecularbiology,Joe.
Sohowdowere/activatemymacrophagessotheygetbacktoworkdestroyingmycancercells?
BygivingyouweeklyinjectionsofGcMAF,tobypasstheproductionblockagecausedbytheNagalase.
Makessensetome.Howbigaretheseshots,doc?
Theyreincrediblysmall.Just100nanogramsthats100billionthsofagram,dissolvedinafewdropsofwater.
Infact,ifyouremovedthewater,theGcMAFalonewouldbetoosmalltosee.
Amazing.
Andthisstuffreallyworks?
Inearlycaseslikeyours,Joe,itworkseverytime.Thatswhyweliketocatchitwhileitsstillsmallusing
Nagalasescreening.
SoyouaregoingtogivemesomeGcMAFshotsthatllperkupmymacrophagessotheycangetbacktowork
findingandgobblingupthesecancercells,right?
Right.Now,pleaserollupyoursleeve.
HowGcMAFworks:GcMAFistheproteinthatactivatesmacrophagesandjumpstartstheentire
immuneresponse.Tosabotagetheimmunesystemandputthemacrophagestosleep,allcancers
andvirusesmakeNagalese,theenzymethatblocksproductionofGcMAF.IntheabsenceofGcMAF,
cancers,HIV,andothervirusescangrowunimpeded.Dr.NobutoYamamotodemonstratedthat
GcMAFadministrationbypassestheNagaleseblockageandreactivatesthemacrophages,which
thenproceedtokillthecancercellsandHIVviruses:
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GcMAFcuresMarysmetastaticbreastcancer
HiMary.
HiDoc.
Goodtoseeyou.Asyoualreadyknow,wehadfoundthatsmallbreastcanceronyourmammogram,andDr.
Humphreyhasalreadytakenitout.
Yup.
Asyoualsoknow,IgotaNagalaselevelbeforethelumpectomy,andthenagainaftersurgery,andthenmonthly
levelssince.BecausetheNagalaselevelisalwaysdirectlyproportionaltotheamountofcancerinyourbody,it
reallyhelpsmetotrackyourcancerandmakesureitdoesntgetoutofcontrol.
Yes,andrememberhowhighitwasrightbeforemysurgery?Then,rightafterthesurgery,itdroppedalot,
becausetherewaslesscancertomaketheNagalase.Butitwasstillelevatedafterthelumpwastakenout,sowe
knewtherestillhadtobesomecancerleftbehindafterthesurgery,darnit.AndthenmyNagalasestarted
climbingagain,soweknewthecancer,whereveritwaswasgrowing.Thatpartwasprettydepressing,doc!
ThatswhyyoustartedmeontheGcMAF,right?
Yes.Andasyouknow,Mary,wevebeentestingyourNagalaselevelsmonthlysincewestartedtheGcMAFshots,
andithasbeengraduallycomingbackdown.ThistoldmethattheGcMAFwasactivatingyourimmunesystem
andthatyourmacrophagesifyoullexcusetheexpressionwereintherekickingbuttonyourcancercells.
Noproblem,doc,itisprettyexciting.
Ivegotsomereallygoodnewsforyoutoday,Mary.YourNagalaselevelhasfinally,after20weeksofinjections,
reachedzero.Youarenowofficiallycancerfree,sowecanstoptheinjections.Andwecancelebrate!Ifyoudont
mindmysayingso,YIPPEE!!!
Wow!Thatsincredible.Youknow,Doc,ImighthavebeendeadrightnowifitwerentforyouandtheGcMAF.
Thankyousomuch!
Mary,itissincerelymypleasure.
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The GcMAF Book
Chapter1
A Cure for Metastatic Cancer?

Canitbetrue?AcureforearlymetastaticcancerandHIVthatappearstowork100%ofthetime
thatalmostnobodyknowsabout?Mysearchforanswers.SolvingtheriddleofGcMAF.WhyGcMAF
remainsobscure.Pleasedontbeintimidatedbycellbiology!Thewaranalogy.
OnNovember19,2008somethingtouchedmylifeandchangeditforever.Ihadjustcompletedmysecondbook,
OutsmartingTheNumberOneKiller(abouthowtopreventandreverseatheroscleroticallydrivenheartattacks
andstrokes)whenIcameacrossthreeseminalstudiespublishedearlierthatyearbyinternationallyrecognized
researchimmunologistandmolecularbiologist,NobutoYamamoto,Ph.D.Thesepivotalpaperswhichwill,I
believe,changethecourseofmedicalhistoryblewmeaway:Yamamotohadapparentlydiscoveredawayto
outsmartcancer,andwasusingthebodysownnaturalhealingsystemstodoit.
Holycow!!!Isaidtomyself(usingawordthatslessbovineandmoregraphic).Thisguyhasdiscoveredacure
forearlymetastaticcancerthatappearstowork100%ofthetime!!!Andthiswasnoblackboxmodelor
statisticalstudy(youknow,thekindthatmakeobservationsbutdontaddressunderlyingcausalmechanisms.
MedicineAcuresdiseaseB,butnothingabouthowitactuallyworks).Allthebasicscience,allthenecessary
molecularbiologicalinformationanimpressivedisplayofpublishedresearchstudiesfromaquartercenturyof
workwasrightthereforalltosee.Nosmokeandmirrors.AnyonewhohasperusedDr.Yamamotosresearch
paperswouldhavetoagreehepublishedanimpressivelyextensiveseriesofserioussciencemasterpieces.
AsIlearnedmoreIbecamecommittedtobringingthispowerfulnewsetofideasintopublicawareness.Itbecame
cleartomethatweneedtofindawaytomakeGcMAFavailabletoallcancer,HIV,andchronicviruspatients,and
weneedtoinstituteroutineannualNagalasetestingtofindcancersmuchearlierthanimagingnowallows.
IfallnewcancersweredetectedearlybyregularNagalasetesting,wecouldreversethemwithGcMAFlong
beforeXrayscouldfindthemandputcanceroutofbusinessonceandforall.Thismayseemlikearash
statement,butIbelieveitissupportedbythefacts.
Yamamotosthreestudiesshowedthatincrediblysmallweeklydoses(100billionthsofagramanamountthatis
invisibletothenakedeye)ofGcMAFhadcuredearlymetastaticbreast,prostate,andcoloncancersin100%of
(nonanemic)patients.Inafourthpaper,heusedthesametreatmenttocure100%ofnonanemicHIVinfected
patients.
Forthethreecancerstudies,Dr.Yamamotohadchosenpatientswhohadrecentlyreceivedthestandard
mainstreamtriadofsurgery,chemo,andradiation.Despitethesetreatments,everypatienthadevidenceof
metastaticdisease,whichmeansthatdespitethebesteffortsofconventionalmedicine,theircancerswereoutof
controlandstillgrowing.Theirprognoseswerepooratbest.Nevertheless,thispatientgrouphadonethingin
common:theirtumormass(alsoknownastumorburden)hadbeendrasticallyreducedbythetherapiestheyhad
received,andthisinturndramaticallyincreasedthelikelihoodthatGcMAFwouldremovethefewremaining
cancercells.
ThesestudiesunderscoretheimportanceoftheuniquecircumstanceinwhichGcMAFismostlikelytobe
effective:verylowtumorburden.Lowtumorburdenoccursinjusttwosituations.Thefirstistheearlieststageof
anycancer,whenthenumberofcancercellsisstillverysmall.Thesecondisimmediatelyafteradiagnosedtumor
hasbeenmaximallydebulkedbystandardtherapiesi.e.,thatwhichprevailedinDr.Yamamotosstudies.
Conversely,thesituationinwhichGcMAFisleastlikelytobeeffectiveiswhenthereisalargenumberoftumor
cells.Althoughresultswillvarygreatlyfromonepatienttoanotherandfurtherstudyisneeded,clinicalresearch
experiencetodatesuggeststhattumorslargerthanonecm.indiameterareunlikelytorespondtoGcMAF
therapy.(SeeChapter18:TheCancercontinuumandthePointofnoreturn_)
Curingmetastaticcanceratallisrare.UntilProfessorYamamotodiscoveredandadministeredGcMAF,noonehad
evercuredeverysinglecase.Thesearethepatientsoncologistsgiveupon,theonesthatgetpalliativecare.
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Perhapsanotherroundortwoofchemoorradiationintheslimhopesofalongtermreversaloralittleextra
(probablynotveryhighquality)timebutwithmetastaticdiseasethereisnoseriousexpectationofanactual
cure.Thenumbersareprofoundlydismal.
Granted,allofYamamotospatientswereintheearliestphaseofmetastaticdiseaseandreceivedGcMAFshortly
aftertheBigThreehadfailed.Forthesepatients(thoughasprinklingmighthavebeensavedbyadditional
radiationand/orchemo),theassumptionwouldusuallybethattheircancerswouldgrowandeventuallykillthem.
GcMAF,remarkably,savedeverysingleone.Thisisanexceptionaloutcomeanddeservesgreaterscrutinythatit
hasreceived.
Afourthstudy,publishedinJanuaryof2009,showedYamamotousingthesametreatmentprotocolhad
removedallsignsofviralactivityin100%ofHIVinfectedpatients.AllpatientswerefreeofHIVwithin18weeks.
Remarkably,Yamamotoaccomplishedthesecuresrelativelyrapidly.Thebreastandprostatecancerpatientswere
allcuredinlessthan6monthsofweeklyGcMAFinjections.Thecolorectalcancerstudytookaboutayeartocure
allsubjects.Fivetosevenyearsofcarefulfollowuprevealednorecurrencesinanyofthepatients.Anyonewhois
familiarwithcancerresearchwouldhavetofindthisremarkable.
Thiswasnotaoneoff,aluckystrike.Yamamotosfourpapersweretheculminationofdecadesoftrailblazing
researchinwhichhehadalreadyprovenviabasicscienceandanimalstudiesexactlyhowGcMAFandNagalase
work.The2008humantrialswerejustthefrostingonaphenomenalcakethattookaquartercenturytobake.
Thebreadthanddepthoftheunderlyingresearchisimportantherebecausemisinformedcriticswhineabout
GcMAFbeingunproved.HadthesenaysayersreadthedozensofYamamotopaperspublishedinpeerreviewed
journalsbetween1979and2008thatlaydownanunimpeachablefoundationforhisfinalproof?Idoubtit.
Mysearchforanswers
WhenIfirstreadYamamotosstudies,Icouldntbelieveiteither.Acureforearlystagemetastaticcancerthats
effectiveineverysinglecase?Absurd.Publishedinpeerreviewedjournals?Noway.Ifiguredtheremustbesome
hitch,amistake,alogicalerror,aweaklink,afatalflaw,andIwasdeterminedtofindit,butthedeeperIdelved
themoreconvincedIbecamethatGcMAFwasforreal!
ThenIstartedwonderingwhyIseemedtobeamongtheveryfewwhogotit.
Atfirst,Ispentahugeamountoftimeenhancingmyunderstandingoftherelevantmolecularbiology,genetics,
andimmunology.IlearnedalotmorethanIeverthoughtIwouldaboutcancer,macrophages,oxidativebursts,
adhesionmolecules,antibodies,phagocytosis,proteinchemistry,cytokines,messengermolecules,receptors,N
acetylgalactosaminidase(Nagalase),andGcMAF.AttimesIfeltasifIhadstuffedsomuchnewinformationinto
myheadthatitwasgoingtoexplode.Ineededtounderstandexactlyhowitallworked,howallthepiecesfit
together.IpouredoverresearcharticlesandmoleculargeneticstextsuntilIfeltIhadareasonablegraspofwhat
Yamamotowasdoingandsaying.Idevelopedtheabilitytovisualizeingreatdetailtheworkingsof
macrophagesbattlingcancercellsandvirusesinthisbrutalmicroscopiccannibalisticwar.
ThemoreIlearnedthemoreitsunkin:usingimpeccablescience,Yamamotohadfoundapowerfulmeansof
enhancingourbodiesownanticancer,antiviralweaponry!Thatswhatcuredthecancers.
ThemoreIlearnedthemoreIaskedthequestion:whyhadthemedicalcommunitymuchlesstheaverage
personneverheardofGcMAF?Noarticleintheprintmedia.Novideo,nobook,noresearcharticlesotherthan
Yamamotos.Noseriousscientificwebdiscussions(whichistrulyextraordinary,becauseeverythingisonthe
internet).Nonothing.
GooglingonGcMAFdoesgeneratesomehits,butnothingsubstantial,noserioussciencebeyondNobuto
Yamamotosownoriginalpapers.Hmmm.Thisistrulyremarkable,Ithought.
NextIturnedtowhatIcallhumanbrowsers.Icalledabunchofmyphysicianbuddiesandmolecularbiology
colleaguesgoodscientistsallbutthere,too,Idrewablankeverysingletime.Notasingleoneofthemhadever
heardofGcMAF.
Noteasilydissuaded,Icontactedseveralimmunologyresearchersatmajorinstitutions,andagainnoonehad
heardofGcMAF.Maybesomeoneinthegovernmentorresearchestablishments,theFDA(FoodandDrug
Administration),theNCI(NationalCancerInstitute),theNIH(NationalInstitutesofHealth),AmericanCancer
Society(ACS)knewsomething?Nope.Noonetherehadeverheardofiteither.(Oriftheyhad,theysurewerent
talking.)
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Inthoseearlymonths,myleveloffrustrationgraduallyescalated.Proofofanaturalcureforadvanced
(metastatic)cancer(nottomentionHIVandotherchronicviralinfections)andnobodyseemedinterested?Ijust
couldntunderstandit.
Finally,asenseofsurrealismsetin.Ihadspenthundredsofhoursonthis,withliterallynothingtoshowforit.
Nobodyknewanything.AndwhenItriedtoexplainYamamotosworktosomeofthepeopleIcalled,Icouldhear
theireyeswereglazingover.Icouldhearthemthinking,Cmonnow,doc.Acureforallcancers?Oh,sure.One
thatworks100%ofthetime?Onmetastaticcancer?Givemeabreak.Itsoundslikesmokeandmirrorstome.
Istartedquestioningmygrasponreality.NoOprah?NoLarryKing?NoNewYorkTimesarticle?Noarticle
anywhere?Nomediacoverageofanykind!Noscientificrecognition?Whattheheckisgoingonhere?
SolvingtheriddleofGcMAF
Afterwritinganentirebookonthesubject,IcanstillhonestlysayIdontknowwhytheaveragepersonnotto
mentiontheaveragephysicianortheaveragemolecularbiologisthasneverheardofGcMAF.Itseemstome
thatthatshouldhavehappenedalongtimeago.Ihopethatsharingthisinformationwillcreatethecriticalmass
weneedtoovercometheobstacles.So:Hellooutthere!HeresacureforcancerandAIDS!!!Evenmore
significantly,heresawaytoridtheplanetofthescourgeofcancer.IampassingonwhatIhavelearnedaboutit
toenableyoutochipinandworkwithmetotransformGcMAFfromasetofabstractconceptstoalifesaving
reality.MillionsuponmillionsofliveswouldbesavedifwecouldmakeGcMAFaharmlessproteinavailableto
themassesofcancerandHIVpatientswhodesperatelyneedit.Andcountlesscancerswouldbepreventedusing
NagalasescreeningandGcMAFtherapyonalladulthumanpopulations.
Pleasehelp!Thisisatwowaystreet.Ivechosenareadereditableformat(actually,truthbeknown,myweb
geniusfriendandcyberguru,PeterRowellcreateditspecificallyforthisbook)wherebyanyonewhoisinterested
cancontributetheirideas.Thebeautyofthisapproachisthatitfacilitatescollectivedevelopmentofideasbyan
organizedcommunity.Justscrollovertheleftverticalgreenbar,clicktoopenadialoguebox,andshareyour
ideas,edits,corrections,andquestions.Indoingso,youwillhaveparticipatedinaprocessthathasthepotential
tohelpalotofyourfellowhumansandtoalleviateahugeamountofsuffering.
Andasifthatwerentenoughofarewardyoullalso(ifyousodesire)belistedintheAcknowledgments.
WhyGcMAFremainsobscure
Hereareafewkeyfactsthatprovideapartialanswertothefascinatingquestion:WhyhasGcMAFgone
unnoticed?
UnderstandingYamamotosworkrequiresafirmgraspofsomeprettyadvancedmolecularbiology,which
mostpeopleevenmostdoctorsandresearchersdontpossess.Itskindofalanguageproblem:ifsomeone
shoutsCancerCure!!!CancerCure!!!CancerCure!!!inSwahili,itisquitepossiblethat
earthshatteringnessofitallwontgetthrough,andeveryonewillgoonabouttheirbusinessasifnothing
happened.(InthisbookIhavetranslatedtheseideasintoeverydaylanguage.Itsnotthatcomplicated.)
Toastodgymedicalcommunitythatsresistanttochange,GcMAFisjustanotherunprovedtherapy.And
analternativeoneatthat.Unprovedtherapiesarenottobetrusted.(Eveniftheyreharmlessand
bioidentical.)
Provingthisdiscoverytheconventionalwaywouldinvolvedevelopingandpromotingalucrativedrug.
Doingthattakesaboutadecadeandcostsover100milliondollars.Beyondtimeandmoney,itrequiresalot
ofbiochemicalknowhowandsomesophisticatedequipment.Brewingitupinyourbasementlabwitha
chemistrysetandabunchofbuddiesisnotanoption.However,amotivatedpharmaceuticalcompanycould
doitovernight.
BigPharmaisntinterestedbecausetheresnocashcowattheendofthisrainbow.GcMAFlikeallchemicals
ourbodyisprogrammedtomakecantbepatentedbecauseitfitstheFDAsdefinitionofnatural
(translation:unpatentable).Wearethusconfrontedwiththesupremepickle:isitpossibletoconduct
openminded,nonprofitdrivenresearchinaneraofcorporatizedandpoliticizedmedicalscience?Iyearnfor
theoldendayswhensciencewasdoneforthesakeofscience.Itwasntthatlongago.)
Thecancerindustrydoesnotreallywantcancertogoaway.Thismayseemharsh,butitstrue.Many
incomeswouldbeinterruptedifcancerandHIVsuddenlyceasedtoexist.Governmentagencieswouldhave
tobeclosed,oncologistswouldhavetoberetrained,researchersredirected,cancertreatmentcentersshut
downorconvertedtoscreeningandpreventionfacilitiesandthatsjustthetipoftheiceberg.Weretalking
profoundsocialupheavalhere.Cancerisentrenchedandinstitutionalized,andvanquishingitwouldcause
majorfireworks.Thesefearsarelargelyunfounded,however.Foroptimumeffectiveness,GcMAFand
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Nagalasetestingwillneedtobeintegratedintotheexistingcancercaresystem,soweneedthesystem.
TounderstandGcMAFandNagalasewemustembraceanentirelynewmodelacompletelydifferent
approachtocancerandchronicviralinfections.Thereisnosuperdrug,nomagicbullet.Ourbodiesalready
knowhowtocurecancerandviralinfectionswesimplyneedtoenhancethesesystemsusingnatural
medicines.ThatshowGcMAFworks.Thescientificcommunity,however,isdeeplyresistanttotheideaof
naturalmedicinesbolsteringtheimmunesystem.
Ifwearegoingtocommittostoppingtheseepidemicsournewdirectionmustbeannualscreening(with
NagalaseorAMAStesting)forearlydetection,thennippingcancerinthebudwithGcMAF.Theoldwaituntil
itsgottensobigwecanseeitonimagingandthenslashandburnitoutapproachhasreallygottogo.
Acouplebriefasideshere,andtheninthenextchapterwellgetintohowGcMAFworks.
BriefasideI:
Pleasedontbeintimidatedbycellbiology!
Totheoutsider,theworldofmolecularbiologyandbiochemistrymayseembewilderingandthelanguageweuse
todescribecellulareventsoftenappearsforeignandincomprehensible.Justbecauseweuseinscrutablycomplex
wordslikeglycoproteinmacrophageactivatingfactor(GcMAF)oralphaNacetylgalactosaminidase(Nagalase)
doesntmeantheconceptsareinaccessible.Theyarent.Pleasedontbeintimidated:thismaterialisnotas
complicatedasitmightseem.Myselfimposedjobdescriptionhasbeentotranslatethisarcane,esoteric,
imposing,andhighlytechnicalscienceintoconceptsthatareeasilyunderstoodbythelaypersonand,inthe
process,tobringittolife.Ifyoufindthematerialhardtounderstand,Ihavefailedatthattask.
BriefasideII:
Thewaranalogy:Iuseitbecauseitworks
ForthesamereasonthatlanguageisthemostpopularandappropriateanalogyfordescribingDNAandgenetics,I
havechosenthewaranalogytodepictthenanoscaledramathatunfoldsontheimmunologicalbattlefield:it
works.
PleasedontthinkthatthismeansIamprowar.Quitetheopposite.Ithinkmostwarsarebadandstupid,andI
detestthem.ThoughIbelievethatsomewarshavebeenjustified(arecentexamplewouldbeWorldWarII,in
whichcontroloftheplanetbydespotswasatstake),Iamonewhoishopefulthatmembersofthehumanrace
canlearntoworkthingsoutwithoutresortingtoneedlessslaughter.Wewillhavereachedahighpointinour
evolutionwhenwelearntoputwarbehindusonceandforall.
Speakingasahumanoidmemberofacellbasedlifeform,however,itiscrucialtoacknowledgethenecessityof
thisinnerwaragainstcancerandmicrobes,thoseevilforcesthatareouttodestroyus.Pacifismwontworkhere
wereallyhavenochoicebuttofightbackordie.
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Chapter2:ProfessorYamamotoandRealScience
The GcMAF Book
Chapter2
Professor Yamamoto and Real Science

Therearethreekindsofresearchstudies.(Actually,therearemanymanykindsofresearchstudies,butforthe
purposeofthisarticle,Immakingitthree.)
First,thereswhatIwillcallblackboxstudies.Inthese,youtrysomethingoutonexperimentalsubjectsand
seewhathappens.Theactualinternalmechanismsdrivingtheresultsarenotnecessarilyknown:Oh,whenwe
fedtheratsmushroomsandgreentea,someoftheircancerswentaway.Interestingandusefuloutcomes,but
causeandeffectareignored.
Secondarepopulationstudiesinwhich(notnecessarilyaccurate)conclusionsaredrawnfromstatisticalstudies
involvinglargegroupsofpeople:Wefoundthat50%ofpeoplewhohadheartattackshadnormalcholesterol
levels.WethereforeconcludethatAgain,underlyingcausativemechanismsarenotpartofthepicture.
Withbothblackboxresearchandstatisticalstudies,theimportanceofcauseandeffectareeffectivelydismissed:
theunderlyingcauseofanobservedeffectisneverknown.Thisisafundamentalflawwecanttrustthe
evidencewegetfromblackboxandstatisticalstudiesbecausetheydontaddresscausality.Forthisreason,Ipay
verylittleattentiontothesefirsttwokindsofresearchstudies.
Thethirdtypeofresearchisbasicscience,whereideasaredevelopedandtestedfromthegroundup.Insights
gainedinonestudybecomethedrivingforcefordesigningthenextone.ProfessorNobutoYamamotodidand
stilldoesbasicscienceresearch.Hespentaquarterofacenturyexamininginminutedetailthebasic
molecularbiologyandimmunologysupportinghisdiscoveries,alphaNacetylgalactosaminidase(Nagalase)and
glycoproteinmacrophageactivatingfactor(GcMAF).Theresnoblackbox,nostatistics,justacomplextrailof
experimentalinsightsthatcollectivelyformacolossalinfrastructureuponwhichhisfinalconclusionsarebased.
Hedidnttryoutanew,unknownandpotentiallytoxicdrugonabunchofpeoplehedidthebasicscience
researchandfiguredoutexactly,precisely,downtothesubmolecularlevelhowcancerbreaksourbodies,and
thenheshowedhowtofixitusingthebodysownsystemsashistherapeutictool.Itsabrilliant,Herculean,
masterpieceofwork,andDr.YamamotodeservesaNobelPrizeforhisefforts.Healsodeservestohavehisideas
widelyunderstoodandtakenseriouslyanoutcomethathasnotyethappened.
Dr.Yamamotoishealthyandactiveatage84.Ifeelthatforhimthebestrewardofallwouldbetosee,inhis
ownlifetime,theworldscancerandHIVpatientsbenefitfromhiswork.
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The GcMAF Book
Chapter3
Your Incredible Immune Army

Yourimmunearmyusesanamazingarrayofweaponstoprotectyoufromcancerandmicrobes.
Therearegoodguys(ourwhitebloodcells)andbadguys(cancerandmicroorganisms)inus,andtheyarefighting
itoutforcontroloftheterritorythatisyou.Iamgoingtoputyouinfrontofaverypowerfulmicroscopesoyou
canseeinvividdetailthisconstantviciousstrugglegoingoninsideeveryoneofus.Theopposingarmies
conductcomplexmilitarymaneuversanddeployafantasticandunimaginablysmallarrayofwarmachinery,all
coordinatedbyasophisticatedcommunicationssystem.
ThebadguystheforeigninvadersareconstantlyattackingusIspeakofallergens,infectiousagents,various
toxins,andcancer.Ourimmunearmy,ourguardian,iscomprisedofdozensofcelltypeswithhundredsof
differentfunctions.Inanysingleperson,immunecellsnumberinthehundredsofbillionsseveraltimesmore
thenthenumberofstarsinourgalaxyorgalaxiesinouruniverse!Theyroutinelysacrificetheirteeny(but
incrediblycomplex)livestodefendus.
Healthyimmunecellsareessentialtooursurvivalinthisongoingwar,andtheyarerelentlessintheirpursuitof
cancerandotherforeigninvaders.Whenactivated,theymakeHitler,Stalin,andMaolooklikeneighborhood
bullies.Immunecellsattackinextremelylargenumbersfarlargerthananyarmyever.Cometothinkofit,
everysinglehumanhasfarmoreimmunewarriorsthanallthearmiesinhistoryputtogether.Theironslaughtis
superblycoordinatedbyanextremelysophisticatedcommunicationandcommandsystemhumanmilitary
intelligenceoperationsarekindergartengamesincomparison.Usingacomplexlanguagecomprisedofmolecular
words(proteins,glycoproteins,cytokines,cellsignalingmolecules,neurotransmitters,etc.),yourimmunecell
soldiersreleaseabarrageofchemicalmessagesthatidentifyforeigninvaders,providetheirlocation,estimate
numbers,andcoordinatetheattack.
Macrophageengulfsandphagocytizesacancercell.FromTheImmuneSystempublishedbyTheUpjohn
Company.
Andwhatanawesomeattack!OurimmunecellssportanarsenalthatwouldmakeStarWarslookpuny.Immune
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cellscanblowupenemyinvaders.Theycanshootoutbeamsofionizedparticlesthatliterallyripholesinthe
outercellmembranesofinfectiousmicrobesandcancercells.Theyreleasespurtsofcorrosivechemicalpoisons.
Theysurround,cannibalize,anddigestenemycellsandthenrecycletheparts.Theylaunchguidedmissilesfrom
greatdistancesthatlandandexplodewithincredibleprecision.Theyevensmothertheirenemiesinstickygoo
(calledcomplement)liketheMarshmallowManinGhostbusters.
Yourimmunearmydeploystheseremarkableweaponsinmultiplewarsonseveralfronts.Theskin,respiratory
system,intestinaltractandbloodstreamsustainthelargestexposuresandthuscontainthemostimmunecells.
Ourlargestexposuretopathogensbyfarisinthegut.Becausethisisthelargestandmostfrequently
breachedbarrier,roughly80%ofyourwhitebloodcellsareembeddedjustbeneaththeintestinalmucosal
surface.Microbesintheformofbacteria,fungi,parasites,viruses,andtheoccasionalhelmintharepersistently
knockingonthedoorandmustbefendedoffonanonstopbasis.Asyouwillsee,wefendwithsomeprettybig
sticks.Imagineanarmywithhundredsofbillionsofwhitebloodcellsoldiers,eachfullyequippedtotakeoncancer
cells,viruses,andbacteriamanoamanoinastruggletothedeath.
Cancercellsareformingcontinuouslyinallofus,butalert,activated(youllsoonseewhyIitalicizethisword)
immunecellswillgivethemtheinstantaneoushatchet.Ifyourconstantlyvigilantandaggressivelyprotective
immunesystemtookacoffeebreak,youdbedeadbythetimeitwasover.
Iamgoingtotellyoualotmoreaboutthewondrous,thoughviolent,molecularbiologicalworldinsideofus.But
first,letstakealookatthebigpicture,anoverviewofourWaronCancer.
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Chapter4:TheWaronCancerInsideUs
The GcMAF Book
Chapter4
The War on Cancer Inside Us

Cancerisawarwagedonasubmicroscopicmolecularscale.Takingakeeninterestinthiswar
wouldntbeabadidea,asitsoutcomewilleventuallydeterminewhetheraboutathirdofuswilllive
ordie.TheadventofNagalasetestingandGcMAFtherapygivesusreasontohopeandexpectthatwe
cansooneradicatethespecterofcancer.Therellbeproblemsalongtheway.
Cancerismanythingstomanypeople.Fromthepointofviewofthecancerpatient,cancermaybeseenasa
fearinducing,lifethreateningnightmare.Fromtheperspectiveofafamilymember,whomaybecalleduponto
provideemotional,physical,and/orfinancialsupport,howcouldcancerbeanythingotherthanstressful?From
theviewpointofthedoctor,whomustfindandtreatthecancertothebestofhisorherability,cancerisbotha
backbreaking,sometimesthankless,jobandanopportunitytoprovideexceptional,lovingpatientcare.
Themolecularbiologist,however,viewscancerasaviciousandprotractedbattlewagedbetweencancercellsand
immunecells.Thiscancerwarastrategicdanceofcellularactivitiesisbreathtakinginitseleganceand
sophistication.
Twocelltypescancercellsandmacrophagescomprisethemainforcesoftheopposingarmies.Bothhave
massivenumbersofpersonnelandawesomeweaponryattheirdisposal,andbothhaveastrategyforwinning.
Remember,thisisWAR!!!Cancercellsaregeneticallyprogrammedtosurviveandspread.Ontheothersideour
immunesystemconsistsofenormousnumbersofcraftywhitebloodcellsdoingtheirbesttodefendus.
Takingakeeninterestinthiswarwouldntbeabadidea,asitsoutcomewilleventuallydeterminewhetherabout
athirdofuswillliveordie.Hundredsofbillionsofcellularwarriorsonbothsideswilldiebeforeitsallover.The
stakesdontgetmuchhigher.Ifthecancer(orvirus)wins,youwillceasetoexist.
GcMAFtreatmentandNagalasetestingwilldramaticallychangethewaythiswarisfought.Withsomehardwork
andperhapsafewluckybreaks,wenowhavereasontohopeandevenexpectthatwecaneradicatecancer.
Notjustinourlifetimes,butinthenextfewyears.
WhenIsaidafewluckybreaksabove,Iwasreferringtoourmedicalcaredeliverysystem,includingthefederal
government,BigPharma,insurancecompanies,cancercarefacilitiesandcancerspecialists.Foramultiplicityof
reasonsincludingbutnotlimitedtotheflowofcashthisgargantuanbeastresistschange.GcMAFandNagalase
arethetwonewelephantsintheroom.Acceptingthatthesebenevolentbehemothshavemovedinforgood
andmakingspaceforthemwithinourcurrentcancercaresystemisgoingtobethechallengeofthecentury.
Peoplewillcontinuetodiebecauseofthereluctanceofmodernmedicinetoembracechange.Ifwestarted
screeningeveryoneforelevatedNagalasetoday,andgivingGcMAFtothosewithhighlevels,wecoulderadicate
cancerliterallyovernight.Leftbehindintherubblewouldbesomesomeveryunhappydrugsalesmenandmore
thanoneboredbutretrainableoncologistwithanoverdueMercedespayment.
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The GcMAF Book
Chapter5
Your Immune Cells Versus The Pathogen Army: A

Nanoscale War
Aboutthenanoscalewarfoughtinsideus.Someimportantfeaturesofcancercells.Itsthecancer
armyvsourimmunearmy.Howmacrostellfriendfromfoe.Macrophagesaretheultimatefighting
machine,buttheyrenaturallyindolentandremainidlewithoutGcMAFactivation.Nagalase,madeby
cancercellsandviruses,putsmacrophagestosleepbyblockingGcMAFproduction.WithoutGcMAF,
macrophagesremaincomatose.GcMAFinjectionsbypassNagalase,reactivatethemacros,andthis
jumpstartstheentireimmunesystem.
Whatiscancer?
Cancersaremadeupofcellsthatcarrydamagedgenes.Thesecellshavestoppedcooperatingwiththerestofthe
cellularcommunity,andaregrowingoutofcontrol.Onceausefulpartofus,andstillharboringourgenome,
thesederangedcellshavenowmutatedintoourarchenemy,hellbentondestroyingus.Lefttotheirowndevices
theywill.Theygrow,forminganisolatedlocalrenegadecommunitymadeupofmalignantcells.Thistumorcan
eventuallysenditscellsthroughthebloodstream(wecallthismetastasis)toestablishsatellitecommunitiesat
remotelocationsinotherorgans.
Thediseasewecallcancerisactuallyananoscalewarfoughtineachofuseverymomentofeveryday.Wenow
knowthatwearealldevelopingcancercellsallofthetime,butthatahealthyimmunesystemdevoursthemas
fastastheyaremade.Anythingthatweakenstheimmunesystemcanshiftthatdelicatebalanceinfavorof
cancer,allowingittogrow.
Someimportantfeaturesofcancercells
CancercellsdontstopreproducingUnlikenormalcells,cancercellsdonotstopreproducingaftertheyhave
doubled50or60times.Thismeansthatacancercellwillgoonandonandondoubling.Soonecell
becomes2,then4,then8,then16.Thecancercellsmaybeabletostopthemselvesselfdestructing.Or
theymayselfdestructmoreslowlythantheyreproduce,sothattheirnumberscontinuetoincrease.
Eventuallyatumorisformedthatismadeupofbillionsofcopiesoftheoriginalcancerouscell.Scientists
describecancercellsasbeingimmortal.
CancercellsdontobeysignalsfromothercellsSomethinginthecancercellsoverridesthenormalsignaling
system.Thismaybebecausethegenesthattellthecelltoreproducekeeponandonfiring.Orbecausethe
genesthatnormallytellthecelltostopreproducinghavebeendamagedorlost.Sothecancercellkeepson
doubling,regardlessofthedamagetheextracellscausetothepartofthebodywherethecancerisgrowing.
CancercellsdontsticktogetherCancercellshavelostthemoleculesontheirsurfacethatkeepnormalcells
intherightplace.Sotheycanbecomedetachedfromtheirneighbors.Thisphenomenonalsohelpsexplain
howcancercellsspreadtootherpartsofthebody.
CancercellsdontspecializetheystayimmatureUnlikenormalcells,ratherthanmaturing,cancercells
becomemoreandmoreprimitive(wecallthisundifferentiated)andtendtoreproduceevermorequicklyand
haphazardly.
Thecancerarmy
Topreventbumpingintooneanother,normalhealthycellsregulatetheirgrowthratebycommunicatingwith
neighboringcellsviaamechanismcalledcellrecognitioninwhicheachcellsendsoutmultiplechemical
messagesinanongoingbiochemicalconversationaheateddiscussionaboutwhogetstogrow(ordoother
thingscellsdo),andwhen.Thereisanetiquetteallcellssubscribeto,andthisverycivilizedmolecularexchange
definitivelysettlesmatterstoeveryonessatisfaction.Cancercells,however,haveoptedout.Theynolonger
agreetodothis.Theynolongerfeeltheneedtoworkoutanagreementaboutterritorialissues.Theywantitall
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now,andrefusetostopgrowing.Havingregressedtobarbarians,theyjustpusheveryoneelseaside.Itswhat
wouldhappenifagroupofcompletesocialidiotscrashedacivilizeddinnerparty,elbowedtheguestsaside,and
startedgrabbingallthefoodforthemselves.Respectfortherulesthatgoverncooperativesocietyisnotontheir
radar.Sayhellotothecancerarmy.
ThecancerarmysmosteffectiveweaponbyfarisNagalase,anenzymemadebyallcancercells(andviruses)
thatshutsdownGcMAFproduction,thusdisablingtheentireimmunesystem.Macrophagesneedtobe
activatedbyGcMAFwithoutit,theyjustcantfight.ByblockingGcMAFproduction,Nagalaseeffectivelycripples
theimmunearmybyputtingitssoldiersintoadeepsleep.(Lateron,Illbegoingintomuchgreaterdetailabout
howthisallworks.)
Yourimmunearmy
Yourbodyalsohasanarmy.Itsmadeupofyourfearlessimmunecells,readytosquareoffagainstthecancer
armyandwageaprotractedwar.Eachsidesfighterscannumberinthehundredsofbillions,butthebattleitself
consistsofhandtohandcombatbetweenindividualmacrophagesandcancercellsinabattletothedeath.The
macrophagesattackandengulf(phagocytize)thecancercells.Thecancercellsaretryingtogrowasfastasthey
can,whilepreventingthemacrophagesfromkillingandeatingthem.
Macrophagesaretheprincipalcellularsoldiersofourhumanimmunearmy.Theyexisttoprotectanddefendus
fromthreatslikecancer.Stationedatcriticalstrategiclocationsaroundyourbody(andembeddedineverytissue
type),thesecellularbehemothsstandguard,readytodeploysophisticatedweaponryshouldaninvaderavirus,
bacteria,fungus,toxin,orallergenoracancercelldaretoer,invade.
Ascellsgo,macrophagesaremassive:roughly30timesthesizeofanaveragebodycell.Ifacancercellwerethe
sizeofamotorscooter,amacrophagewouldbebiggerthanan18wheelsemi.Butthiswouldbenoordinary
truck.MorelikeaShermantank,themacrocytecomesarmedtotheteethwithamultiplicityofnastyweapons.
Italsoknowshowtotellfriendfromfoeandifyourefoe,preparetobemoreorlessinstantaneouslyliquidated.
IntheirquesttokillcancercellsandHIVvirions,macrophagesarentshyaboutdeployingtheirarrayofhightech
devices.Theyextrudeclustersoflongskinnypowerfuloctopuslikearmsthatgrabontoandthendragtheirvictim
in,whereupontheysurround,engulf,anddigestit.Theyemitfreeradicalraysthatfrytheoutercellmembranes
ofcancercellsandmicrobestheyvetrackeddown.Thentheycasuallyspitoutthedeadpartsandmoveontothe
nextcancercell,bacteria,orvirus.
Utilizingsophisticatedsurveillanceandcommunicationsystems,macrosexchangecomplexmessageswithBand
Tlymphocytecells.Outgoingmessengermoleculestelllymphocyteswhereandhowtoaimtheirantibodiesand
inflammatoryresponses,andincomingmessageshelpthemacroszeroinontheenemy,providingspecific
directionsaboutwhereandhowtodirecttheirintimidatingweaponry.
Howmacrostellfriendfromfoe
Becauseitisveryimportantthatthesekillingmachinesnotaccidentallyattackourowncells(friendlyfire),they
areequippedwithasystemfordistinguishingselffromother.ItsanIDcheck,notunlikelikepoliceusewhen
theyinspectadriverslicenseataroutinetrafficstop.Firstthemacrophagesendsoutacellextension,along
protoplasmicarmthatwrapsaroundpotentialtargets(whichareoftenidentifiedbyacloakofstickyIgG
antibodies),puttingthemintoastrongheadlock.Thearmthencontractsalittle,pullingtheintrudercloser,in
ordertobeabletofriskit.(Likeacopgrabbingafleeingsuspect.)Selfcellshaveamolecularsurfaceprotein
(theirdriverslicense)thattellsthemacrophage,Friendherepleaseletgoofme.Themacrophageresponds
byswitchingofftheheadlock,allowingtheselfcelltomoveon.Foreigncells,i.e.,thosewithouttheprotein
license,suffertheignominiousfateofbeingnotjustapprehended,butalsosummarilyeatenalive.Noticket,no
trial,nojudge,nojurythisisrawcellularvigilantejustice.
Imagine,ifyoucan,literallybillionsofthesemacrophagewarmachinessimultaneouslytrackingdown,
apprehendinganddispatchingmalignantcellsinsideofacancerpatient.
Theoutcomeofthismicroscopicwarwillbedecidedbyonesimplestrategicfact:whetherornotthecancerarmy
cansuccessfullyincapacitateourmacrophagesoldiers,becauseifthishappens,thecancerwillprevail.
Macrophagesarecunningandscarybutnaturallyindolent
Macrophagesaretheultimatefightingmachine.Buttheyarenotactuallyamachine:theyarealivethey
thinktheymakewellinformeddecisions.EachpossessesacopyofyourDNA,completewithall25,000(orso)
genes,andthismeanstheyhavethecapacitytosynthesizetensofthousandsofchemicalswhatevertheyneed
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tofightthewar.
But,asweshallseeinamoment,macrophagesarenaturalslackerswithoutakickinthederriere,theyllstay
asleep.Thatkick,themotivationtogetupandgetgoing,comesintheformofGcMAF,aproteinsynthesizedby
ourlymphocytes.GcMAFattachestoreceptorsonthesurfaceofthemacrophagesandsendsapowerfulmessage:
GettoworkNOW!!!
Twomacrophages(left,brown)ensnaringbacteria(blue)intheirlongpseudopods.(EncyclopediaBritannica)
Nagalase(madebycancercellsandviruses)putsmacrophagestosleep
Themainweapondeployedbycancercellsandvirusparticlestosabotageourmacrophagedrivenimmune
responseisanenzymewithatonguetwistingname:alphaNacetylgalactosaminidase.WecallitNagalasefor
short.NagalasedefeatsourimmunesystembyblockingGcMAFproduction.WithoutGcMAF,macrosremain
comatose.Withoutmacrophagestostopthem,pathogenicinvaderscangrowatwill.WithNagalaseontheirside,
cancercellsandviruseswillmultiplyandtheywillspread.Inchapters9,10,and11IllexplainhowNagalase
accomplishestheremarkablefeatofoutmaneuveringandzombifyingourotherwiseintimidatingmacrophage
warriors.
MacrophagesremainidlewithoutGcMAFactivation
Inordertorealizetheyaresupposedtogooutanddestroycancercells,macrophagesmustbeactivated.GcMAF
(standsforglycoproteinmacrophageactivatingfactor)isourbodiesprincipalmacrophageactivatingfactor.GcMAF
isaproteinthatismadeandreleasedintothebloodstreambyyourTandBlymphocytes.Macrophagesurface
receptorsmonitorincomingchemicalmessages,patientlywaitingforspecificorderstoactivate.Muchasatiny
keyfitsintoaverysmalllock,GcMAFmoleculeslocateandlockontospecificGcMAFreceptorsontheoutersurface
ofyourmacrophages.Insertingthekey(GcMAF)intothelock(GcMAFreceptor)unleashesapowerfulalarmthat
isinstantaneouslyheardeverywhereinsidethatcell(eventhoughthecellismillionsoftimeslargerthatthe
GcMAFmolecule!)Thoughtherearesomeweakerchemicalmessengersthatcangivealittlenudgetoward
activation,whenGcMAFcomesalong,macrophagesreallypayattention.Withvolumeturnedallthewayup,
GcMAFshouts,Getgoing!!!!Trackdownandkillallcancercells.Killallviruses!!!DOITNOW!!!Itskindoflike
adrillsergeantbarkingcommandsinbootcamp.OragentJackBauermobilizingCounterTerroristUnitfield
operationsin24.
Basedonsizealone,thisLilliputianGcMAFdictatorhasmorepowerthanthedirectoroftheFBIorCSI.Andthe
signalisnotarequestitsacommand.Thisispowerandleveragelikethesingleswitchthatturnsonallthe
lightsinafootballstadium.WhenGcMAFtalks,macrophageslisten.
WhenamacrophageisunderthecontrolofGcMAF,itsactivitylevelincreasesbyafactorof30.Ifitwerean
automobile,themacrowouldacceleratefrom5to150milesperhour.Butwerenotjusttalkingvelocityherewe
are,moreimportantly,talkingaboutanexplosionofactivity.ThesafetyisoffBigMacisnowarmed,readyto
dobattle,andactivelyseekingengagementwiththeenemy.Therateatwhichthisnowactivatedmacrophage
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canseek,grab,andphagocytizealienlifeforms(andothernonlivingforeignintruders,liketoxinsandallergens)is
aweinspiring.
GcMAFbypassesNagalaseandreactivatesmacros.
OnceactivatedbyGcMAF,macrophagesmorphintorelentlesskillers.ButwhendeactivatedbyNagalase,they
slowtoacrawlandrefusetodeploytheirawesomearrayofweaponryTheystartlosingthewaroncancersand
microbes.Ifmacrophagesremaindeactivated,thepatientwilleventuallydie.InjectingGcMAFbypassesthe
productionblockage,andreactivatesthemacros.
ThefollowingchartsfromDr.YamamotosearlymetastaticcancerandHIVstudiesillustrateshowGcMAF
injectionsbypasstheNagalaseproductionblockageandcurecancer.Patientsreceivedweeklyinjectionsof100
nanogramsofGcMAF.TheGcMAFactivatedmacrophagesdestroyedthepathogens(cancercellsandvirus
particles),thusloweringtheamountofNagalasetheymake.WeeklyNagalasereacheshealthycontrollevelsinall
cases,indicatingthepatientsarecured.
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The GcMAF Book
Chapter6
Your Awesome Macrophage Killing Machine

Macrophagesarebigandsmartwhitebloodcellsthatchase,capture,engulf,anddigestintruders.
Theytrapandphagocytize(literally,eat)theirenemies.Theycanmultiplyrapidlywhennecessary.
However,theyrenaturallyindolentandneedtobeactivatedbyGcMAF.Opsoninsupergluehelps
themsticktotheirprey.Theirelectrondrivenfreeradicaldeathray(AKAoxidativeburst)blasts
holesinmicrobesandcancercells.Onceamicrobeorcancercellhasbeenphagocytizedbyamacro,
itisencapsulatedinsideaphagolysosome(theintracellulardeathchamber),whereitisthen
killed(ifitisntdeadalready),andthendissecteddownintoitscomponentparts,whicharethen
recycled.
AlthoughIhavealreadydescribedmacrophages,theselargeimmunecellsaresoimportantintermsof
understandinghowGcMAFworksthatIneedtogointoalittlemoredetailaboutthem.Besides,theyaretruly
fascinatingcritters!
Amacrophage
Ifyoucouldimaginealiving,breathing,oozing,cunninglyhorrifichumongousstickyblobthatcombinesthe
dangerouslydiabolicalfeaturesofKingKongsintimidatingsize,HannibalLechterscannibalism,DarthVaders
lightsaber,TheTerminator,BruceLee,andtheMarshmallowManthattakesoverNewYorkCityinGhostbusters
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allrolledintoonegiantkillingmachineyoudhavesomeideaofwhatamacrophageisallabout.Theyrebig.
Theyrenasty.Ifyouwereabacteria,virus,orcancercell,youwoulddoyourdarnedesttoavoidthem.
Ascellsgo,macrophagesarehuge
HowbigisaBigMac?Bywayofcomparison,redbloodcells,whitebloodcells,andtypicalcancercellsareabout
7microns(micrometersormillionthsofameter)indiameterandhaveavolumeofabout250cubicmicrons.At
about20micrometers(20millionthsofameter)indiameter,macrophagesareaboutthreetimesaswideas
regularcells.But,becausealittleextrawidthtranslatesintoalotofextravolume,macrophagesataround4000
cubicmicronshaveabout16timesthevolumeofnormalsizedcells.IfacancercellwerethesizeofaToyota
pickup,amacrophagewouldbebiggerthanan18wheeler.
Notadumbtruck
Butthisbehemothisnotadumbtruck.Bristlingwithweaponry,itsstuffedtothegillswithadauntingarrayof
hightechsystemsprogrammedforasingularpurpose:takeouttheenemyasquicklyandefficientlyaspossible.
Wecallthistumoricidalcapacity.
Hereshowitworks.Whenitisntswimminginthebloodstream,amacrophagecanslowlywalkthrough
tissuesusingselfgeneratedstumpylittle(onemicron)legs(abouttenofthemsproutatatime).The
macrophageamblesovertoandsnugglesupalongsideaforeigninvader(e.g.,cancercellorvirion),quickly
identifiesitasfoe,spraysitwithmembranefryingfreeradicalladenDarthVaderdeathbeams,grabs,engulfs,
smothers,kills,anddigestsit.Iftheenemyisfurtheraway,ortryingtoescape,themacrochasesafterit,
extrudesaclusteroflongthinstickyspaghettiliketentaclesthatwraparoundandensnarethefugitivecell,
clutchingitinanunbreakablestranglehold.
Inaprocessknownasphagocytosis,themacrodrawsinitsvictim,engulfsandsmothersit,thenencasesitina
smallbubblelikecyst(calledaphagolysosome)insideitscytoplasm.Thephagolysosomethensecretesacocktail
ofcorrosivefreeradicalsandenzymesthatrapidlydigestitsvictimdownintoitscomponentparts(aminoacids,
nucleicacids,fattyacids,etc.).Themacrophagethenspitsoutthesepiecesintotheintercellularsoup.Because
theremnantsofvirusesandcancercellsarefundamentalcellularbuildingblocks,thebodyquicklyrecyclesthem
usingthesparepartstobuildbrandnewhealthycells.
Ifindingittotallyamazingthatthiscomplexandtrulyviolentscenarioisunfoldinginyouandmebillionsoftimes
perminute.
Areviewofthemacrophagesmostimportantweapons:
Pseudopods
Literallyfalselegs.Thesecanbeshortandstumpy,e.g.,liketheonesmacrosmakeinordertowalkalongthe
innerliningofbloodvessels.Forchasingandgrabbingfugitives,however,macroscanmakemuchlonger
pseudopodsthatextendoutrelativelylargedistances(perhaps60microns).Imagineamacrothesizeofa
Volkswagenwiththecapabilitytoextendhundredsoflongthinarms(eachaboutthediameterofanexhaust
pipe)outto50feetormore.Onceoutthere,theycanweavethemselvesintoanetthattanglesaroundand
trapsthehaplessenemy.Ifthattargetwereacancercell,itwouldbeaboutthesizeofamotorscooter.Ifa
bacterium,itwouldbeaboutthesizeofarollerskate.
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Macrophageensnaringbacteria
Phagocytosisandphagolysosomeformation
Oncethepseudopodshaveensnaredtheirvictim,theengulfingprocessensues.Theoutermembranesofthe
pseudopodsnearestthemicrobeorcancercellsimplymergeintooneanothersothatthevictimiscompletely
surroundedandencapsulatedinwhatiscalledaphagolysosome.(Phagomeanseat,lysomeansdigest,
andsomemeanscellorbody.)Amoebalike,themacrophagehasreshapeditselfsuchthatthe
phagolysosomeliesdeepinside.Thenthemembranethatmakesupthewallsurroundingthephagolysosome
shootsmoredeathraysatitscapturedprey(justtomakesureitisdead),andproceedstodigestitwithanarray
ofcorrosiveenzymes.Moreaboutphagolysosomesinaminute.
Toseeacoolvideoofawhitebloodcell(aneutrophil)chasingandphagocytizingabacteria,gohere:
http://www.youtube.com/watch?v=MgVPLNu_Sw&NR=1
(Thisisaneutrophil,notamacrophageamacrophagewouldbeabout16timeslarger.)
Opsonins:SuperGluebindingenhancersthathelpmacroslatchontoenemies
Tohelpthemgrabandholdtheirvictims,macrophagessendsignalstonearbylymphocytes,instructingthemto
sprayathincoatingofstickyproteinsontopotentialprey.Then,whenthemacroslongthinarmsmakecontact
withthemicrobeorcancercell,thissupergluecoatinghardens,makingitimpossibleforthedesperadotoshake
loose.
Typically,amacrosendsoutaclusterof(saytwentyorso)stickypseudopodsthatsurroundtheenemycell,
encasingitinameshlikeaffair,notunlikealargefishnet,inwhichthemicrobeorcancercellbecomesensnared.
Likeaflyinflypaper,theenemycellisbothstuckinitandtoit,sothereisnowaytogetloose.Thenthepreyis
graduallysurroundedandengulfed,endingupsnuglyinsidethemacroasaphagolysosome,inwhichitspendsits
lastfewmomentsasalifeformbeforebeingdigesteddownintoitscomponentpartsbyvariousfreeradicalsand
enzymes.
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Thestickyproteinsarecalledbindingenhancersoropsonins.Thegluingprocessiscalledopsonization.
Interestingly,whenamacrograbsanenemythisway,itwantsitsfellowphagocyticsoldierstoknowpreyis
nearby,solikeanisolatedsoldierwhohasstumbleduponagroupofenemytroopsandiscallingforbackupit
sendsoutproteinsignalstellingnearbymacrophagestomakemoreofthereceptorsthatspecializeingrabbing
specificallythatkindofenemy.Theressafetyinnumbers.(Technicallythisiscalledupregulatingexpressionof
complementreceptorsonneighboringphagocytes.)
Electronsdeathraybeamsfromtheoxidativeburst
BecauseitissodiabolicallysophisticatedandrightoutofStarWarsmyfavoritemacrophageweaponisthe
oxidativeburst(alsowidelyknownastherespiratoryburst).ThisistheDarthVaderdeathray.Anenzyme
(calledNADPHoxidase)stationedinthemacrosoutermembranespraysoutabeamofhighlyreactivefree
electrons,likebulletsfromamachinegun.
RememberthoseoldTVsetswithpicturetubes?TheNADPHguniskindoflikethat.Atthebackofthetubean
electrongunaimedparticlesthathitphosphorescentparticlesonthescreen.Whentheelectronbeamhitthe
particles,thescreenlitup,creatingapicture.Likewise,NADPHalsoemitsaparticlebeam.Butinsteadofplaying
HowdyDoody,itsblastingtumorcellsandmicrobestosmithereens.
Theelectronsinthebeamemergeoneatatime,buttheyreallyreallydontwanttobefree,soasfastasthey
possiblycantheysnatchanotherelectrontoformastablepair(wearetalkingnanosecondshere).Achain
reactionofelectronsnatchingstriggeredbytheoxidativeburstliterallyvaporizesmoleculesintheouterwallofa
cancercellorviralcapsid,rippingholesinit.Nowthemembranethatheldthevictimtogetherliterallyfallsapart,
spillingoutitscontents.Withoutanintactoutermembrane,acancercellcantsurviveforverylong.
Oxidativeburstsdonthappenallofthetime.Thatwouldbeawasteoffirepower.Thetriggerthatturnsitonis
theperceivedproximityofafoe,acancercell,HIVvirus,hepatitisvirus,orabacterium.Whenamacrocomes
intoimmediatecontactwithenemy,thenandonlythendoesitturnontheelectrondeathbeam.
Therearelotsofoxygen(O2)moleculeseverywhereinourbodies.(Weneedplentyofoxygenandglucose,the
fuelsfromwhichwegeneratetheenergythatdrivesallofthecellularchemicalreactionsthatmakelife
possible.)Whenreleased,mostoftheelectronsinthedeathraybeamcrashintooneoftheseomnipresent
oxygenmolecules,fromwhichtheyquicklygrabtheelectrontheyneedtomakeastablepair.Theoxygen
moleculenowismissingoneofitselectrons,andisthustransformedintotheviolentlycorrosivefreeradical
knownassuperoxide(O2).Nowsuperoxideistheonewantinganelectron,anditwilldestroyanythinginits
pathtogetone.Thatanythingwouldbethevirus,bacterium,orcancercellourmacrohasgrabbedwithits
pseudopod.Suddenlytheinvaderfindsitselfwithahugeholeinitsoutermembrane.Itlldiesoon.
Thefreeelectronsandsuperoxidesalsotriggerchainreactionsformingotherreactivefreeradicalspecies.Oneof
theseisthehydroxylion(OH).Thisishydrogenperoxide,justlikethestuffthatcomesoutofthatbrownbottle,
but33timesaspotentalocallygeneratedintercellulardose.Perfectforfryingmicrobesandtumorcells.
Byoxidizingomnipresentchlorineatoms,theelectronbeamalsogeneratesnoxioushypochlorousacid(HClO),
whichcanpokeaholeinanenemymembraneinnothingflat.Nowwehaveatoxicsoupoffreeradicaloxidizing
agentsthatcandotremendouslocaldamagetoourenemies.
Waitaminute,(Icanhearyousaying,)howcomeourowncellsarentdamagedbyfriendlyfire?Howdothey
escapethedeathrays?Greatquestion.Wehaveaprotectiveshieldthatpreventsthefreeelectronsandfree
radicalsfromdamagingourowncells.ItscalledSOD(superoxidedismutase)anditsanenzyme(alargeprotein
molecule)thatspecializesinneutralizingsuperoxideandotherfreeradicalsbeforetheycandamageourowncells.
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Formaximumprotection,SODispositionedrightnexttotheNADPHdeathraygeneratorproteinsintheoutercell
wall(ormembrane)ofourmacrophages.
Thebarrel(theNADPHmolecule)ofthemacroselectrongeneratingdeathraygunisaimedtowardtheoutside
ofthecellandsticksoutofalittleholethatissurroundedandprotectedbymoleculesofSOD,formingakindof
bunkertoprotecttheelectrongunandyourmacrophagecell.AslongaswekeepmakingSOD(andwedbe
deadinminutesifwestopped),wellbesafetheelectronbeamcantharmus.Itsaprettycoolcombination:a
ridiculouslydeadlyweaponwithbuiltinsafeguardsfortheuser(thatwouldbeyou).
GcMAFActivatedMacrosandtheOxidativeBurst
Youveheardthisbefore,butIhavetosayitagain:onlyGcMAFactivatedmacrosaregoingtodeliveroxidative
burststhatarepotentenoughtobeeffective.IfNagalasefromvirusesorcancercellshasputthemacrosto
sleep,theoxidativeburstdegeneratesintoapiddlypotatogunthatsnotgoingtohurtanybody.Firepoweror
lackthereofiswhatwearetalkingabouthere.Rememberthoseoldwesternsinwhichsixshooterswerethe
mainweapon?Theredbeashothere,longpauses,andthenanothershotoverthere?Therewasalongenough
gapbetweenshotsthatyoucouldactuallyhearthericochets.Thatsadeactivatedmac:slowatthedrawand
notgettingverymanyshotsoff.Reloadingaftereverysixshots.NowondertheIndianscreamedCuster.
Activatedmacrosfiretheatomicequivalentofmillionsofroundsasecondandneverhavetopausetoreload.
Somenewermovieshavesomanybulletsflyingfromsomanydirectionsthatitishardtounderstandhow
anyonecouldsurvive.ThatsfirepowerofthesortonlyGcMAFactivatedmacrophagescoulddeliver.
ThePhagolysosomeexecution(anddismantling)chamber
If,somehow,amicrobeorcancercellhassurvivedtheoxidativeburstandphagocytosis,itwillnotsurvivethe
deathchamber.Onceeaten,internalized,andembeddedinthemacrophagescytoplasm,theenemyisimprisoned
inaroundcystlikebubbleinsidethemacrophage(calledaphagolysosome)intowhicharesquirtedallsortsof
digestiveenzymesandmanymoreroundsofoxidativeburst,justforgoodmeasure.Prettythingsdonothappen
insideofphagolysosomes.Ifthecancercellormicrobeisnotalreadydead,thephagolysosomedeathchamber
willcertainlypolishitoff.(Phagomeanstoeat.Lysomeanstodissolve.Somemeanssackorbag.)
Oncethedismemberingprocessiscomplete,thephagolysosomeslidesoverandmakescontactwiththeoutercell
membrane,mergeswithit,thendisgorgesthenowharmlessbreakdownproducts(nucleicacids,fattyacids,
aminoacids,etc.)outintotheextracellularfluid.Theyarethentakenupbynearbycellsandrecycledintonew
bodyparts.Theecologicallymindedamongusshouldfindtheefficiencyofthisprocesscommendable.Nothingis
wasted.Scarytoxicbadguysarekilled,dismantled,andtransformedintosparepartsforthegoodguys:us.
Asophisticatedcommunicationsystem
Talkaboutcommunicationsystems!Immunecellsmacrophagesandlymphocytescarryonaconstantblather,
likeahugetownhallchatroomwhereeverybodyistalkingatonce.However,sincethetalkingisareleaseof
messengermoleculesandthelisteningisdonebyproteinreceptors,immunecellscanactuallylistenwhilethey
aretalking!!Noneedtocomplainaboutbeinginterrupted!Itsweird,andforeigntoushumans,butthis
simultaneoustalkingandlisteningmakesforafarfasterexchangeofmessagesthanifyouhadtostopandlisten
everytimetheotherguywastalking(likewehumansusuallydo).
Thereissomuchactivity,whatwiththeconstantmolecularchattercoupledwithamadhouseofcellular
scramblingtograbandkillenemycellsasrapidlyaspossible,thatthecasualobservermightgettheimpressionof
chaos.Butshewouldbesadlymistaken.Therearenowastedeffortshere.LikeaBeethovensymphony,
everythingisextremelywellorganizedandperfectlycoordinated.
Thechemicalchatteramongmacrophagesandotherimmunecellsissorapidandefficientthatitwouldmakea
sophisticatedmilitarycommunicationssystemlooklikeabunchofkidswithtincanphones.Macrosreleaseclouds
ofmessengermolecules(cytokines,interferons,leukotrienes,andothersmallmolecules)atratesofupto
thousandsofmoleculespersecondpercell.Eachmoleculecarriesaspecificrequestorcommand.LikeBringme
this,orWeneedsomeofthatoverthere,orKilleverythingthatlookslikethis.Weneedaninflammatory
responseoverhere.OrWedontneedtodothatanymore.Theydiscusswhattheenemylookslikeandhow
aggressiveheis.Theytelleachotherhowhardtowork.Theylabeltargetsforothercellstoidentifyandkill.They
talkaboutwheretheenemyishiding.Theydiscusscurrentenemystrategyandhowbesttooutmaneuverit.
Exponentialselfcloning:theultimateweapon
Last,butdefinitelynotleast,macrosifoutgunnedplaythepopulationcard:theymultiplyrapidly.Whenthey
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findthemselvesinanareaofhighcancercellorviralparticledensity,theydonthavetocallupthedrafttoget
moretroopstheysimplyclonethemselves,whichtheycandoonveryshortnotice.Moremacrosautomatically
translatesintomoreofalltheotherweaponsenumeratedabove.But,again,thismultiplicationprocessoccurs
onlyinactivatedmacros.
GcMAFActivation
WithoutGcMAF,macroslanguish.InthepresenceofGcMAF,theiractivitylevelincreasesexponentially.Once
activated,macrosmultiplyrapidlyandattackferociously.Inthefollowingchapter,Iexplainwhy
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The GcMAF Book
Chapter7
Macrophages Need GcMAF to Thrive

Despitetheirintimidatingsizeandmultitudeofnastyweapons,macrophageslanguishwithout
GcMAFactivation.Quantifyingimmuneactivation.Macrosuseantigenpresentingmessengersto
reverseimmunosuppressionandactivateotherimmunecells.ElevatedNagalaseblocksGcMAF
drivenmacrophageactivation,resultinginimmunosuppression.
Ithinkyoudhavetoadmitthatmacrophagesareprettyamazingandintimidatingcharacters.Expandedto
humanscale,theywouldbefarsuperiortoanyexistingweapon.Theydbeabletoblowtheirwaythrough
cementwalls,devourmoosesizedanimalswholeandgrabstuffthatwasfiftyfeetawaywithaclusteroftheir
longstrongadhocarms.Butaswithmostthingsthatseemtoogoodtobetruetheresahitch.Despitetheir
incrediblepotentialtoprotectanddefendus,macrophagesarenaturallyindolent.Theseslackerssleepuntilnoon
(figurativelyspeaking),andhangoutinthebloodstreamlikeabeachbumattheseashore.Itmightbemore
generoustosaythatintheirnaturalstatetheyareoffduty.Macrosaccomplishabsolutelynothingbeyond
eatingenoughtostayalive,andindulgingintheoccasionalreplicationexperience.Aninactive(ordeactivated)
macrophagelacksthekillerinstinctandmightfloatrightpastahugegatheringofcancercellsorsomepartying
virusparticles,oblivioustotheirpresence.Notexactlyakillingmachinemorelikeagargantuanlifelessblobof
coldgooeymarshmallow.
Togetmotivated,theseloafersneedaswiftkickinthebutt.Becauseweareseriousscientists,however,wehave
tocallthismacrophageactivation.Itisaccomplishedbyspecificactivatorproteinsthatattachtoreceptorson
themacrophagessurface,notunlikethetoeofaboot(theactivatorprotein)beingembeddedinthebutt(the
receptor)ofaslacker(themacrophage).Thoughseveraltypesofmoleculesareknowntogivemacrosagentle
shove,GcMAFisbyfarthemostpotentmacrophageactivatingfactor.Infact,nothingcomesclose.
WhenGcMAFactivatesmacrophages
AsGcMAFdocksonitsreceptorsontheoutersurfaceofthemacrophage,itsendsasignaltotheentirecell,telling
ittobecomeaggressive,accelerateallactivitiestowarpspeed,andprepareforbattle.Ourlaidback
beachcombersmorphintofrenziedkamikazewarriors.Wearenottalkingaboutanudgeheretheword
activationmaybetooweaktodescribeaphenomenonthatconsistsofa30foldincreaseinmacrophage
activity.ImagineaModelTputtingalongat20mphsuddenlytransformingintoajetplanedoing600!!!Youget
theidea.
GcMAFactivationtransformsmacrophagesmoreorlessinstantaneously(okay,ittakesabout3.5hours)intoa
superconquerorthatvanquishescancercellsandvirionsataphenomenalrate.GcMAFrestoresthetumoricidal
capacityofmacrophagesitsabilitytorapidlyscarfandkillvirusesandcancercellsthathadbeenobstructedby
theNagalase.Battalionsofactivatedmacrosnowtrackdownandchewtheirwaythroughenemyarmies,spewing
outdeadpartsalongtheroadsidebehindthemsofasttheymakeitlookeasy.
Quantifyingmacrophageactivation
Dr.Yamamotoquantifiedseveralspecificmeasuresofmacrophageactivation.ComparingdeactivatedtoGcMAF
reactivatedmacros,heobserveda30foldincreaseinrateoftumorcellingestion(phagocytosis),a15fold
increaseintheoxidativeburst(asuddenreleaseofsuperoxide(O2)ionsthatzapscancercells,bacteria,and
viruses),a40foldincreaseinsystemicmacrophagecounts,andanexponential180foldriseinmacrophage
levelsininflamedlesions.(Thelatterbecauseactivatedmacros,viaaprocessknownaschemotaxisareattracted
toward,andmigrate,toareasofinflammatione.g.,cancersandviralinfections.)
Weeklyinjectionsof100ng(100billionthsofagram)unleashesarmieswithbillionsoftheseGcMAFactivated
clonedmacrophagesthatrelentlesslyattackandgraduallyannihilatesmalltumorswithinaboutsixmonths.(Dr.
Yamamotosmetastaticbreast,colonandprostatecancercaseswereallputintoafiveyearremissionifnot
curedwithinthistimeframe.)
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Largertumorsabiggermealforthemacros,sotospeakpresentmoreofachallenge.Here,resultswill
certainlyvaryfromonepatienttothenext,andsomewillnotbecurable.Wereallydontyethaveharddataon
largermasses.Themacroswillcertainlydotheirbestifactivatedbutforeachpatientthereisgoingtobea
pointofnoreturnwherethetumorhasgottensobig(ortheviralloadsolarge)thatitcansuccessfullyfendoff
andstayaheadofthebesteffortsoftheactivatedmacros.
Activatedmacrosuseantigenpresentingmessengerstoreverseimmunosuppression
Likethatproudmousercatwhoinsistsondroppingdeadrodentsathismastersfeettoproveheisearninghis
keep,macrophages,inastrange,microscopicsense,aresimilarlyproudofthefactthattheyhaveidentified,
trapped,phagocytized,killed,anddismemberedadesperado,beitcancercell,bacterium,orviralparticle.So,to
spreadthewordoftheirsuccesstotheirimmunecellcomrades,macrosreleasetriumphantcloudsofmessenger
molecules.
Macrophagesalsowanttopassonidentityinformationtootherimmunecellssothateverybodyelsecanmore
easilyidentifythebadguys.Toaccomplishthis,macrosbiochemicallypaintthemolecularbiologicalequivalentofa
hugeluminescentXonscragglypiecesofleftoverdetritusthat(priortodismembermentinitsphagolysosome)
hadbeenpartofacancercelloravirus.Oncereleasedintotheintercellularfluid,thesebrightlylabeledpartsfloat
offlikeamessageinabottlethat,whendiscoveredbyanotherimmunecell,tellsittoKeepaneyeoutfor
anythingresemblingthisguyandwhenyouseeit,killit.
Otherimmunecellsfindthebottles,readthemessages,andinstantaneouslyknowandrememberthatthisis
thekindofcelltheyshouldbetargeting.Wordspreadsfastamongphagocytes.Sincelivingcancercellscontain
thesesamepartsasthosemarkedwiththeX,lymphocytes,othermacrophages,andotherimmunecellsnow
caninstantaneouslyrecognizetheenemyandtakethemoutwithoutfurtherado.Wecallthisantigen
presenting,anditservesadualpurpose:firstitspreadsthewordaboutwhomtotarget,andsecond,ittransfers
theactivationofmacrostoallotherimmunecells.
ElevatedNagalaseissynonymouswithimmunosuppression
Ifmacrosarentactivated,antigenpresenting(andallotherimmunefunctions)slowstoacrawl.Cancersand
viralinfectionsdeactivatemacrophagesbymanufacturingandreleasingNagalase(alphaN
acetylgalactosaminidase),theenzymethatpreventsGcMAFproduction.
WithoutGcMAF(andwithNagalase),theentireimmunesystemhasdroppedout.Theyverevertedtoacollection
ofstagnant,indolent,ineffectualcellsallgonefishin.Theendresultisstagnationgeneral
immunosuppressionandanenvironmentinwhichcancersandvirusesgetahugegreenlight.
Deactivatedmacrophagesmalfunctioninnumerousways.Phagocyticactivitydramaticallydeclines.Nowno
invadersarebeingeaten,sonoluminouslylabeledtumororbugpartsarebeingspitout,soantigenpresenting
screechestoahalt.
Withoutantigenpresenting,therestoftheimmunesystemwontbewarnedthatinvadersarenearby,soit
remainsdormant.Asyoucansee,thedeactivationitselfperpetuatesmoredeactivationitsaviciouscycle.Soall
theotherimmunecellssitidlybywaitingformarchingordersthatnevercome.Theentirecascadethatleadsto
rampedupimmuneactivityisblockedwhenmacrosarenotbeingactivatedbyGcMAF.
Fromthemacrophagesperspective,thisisanightmarescenario.LikeGulliver,ithasbeentieddownand
immobilizedbyhordesofLilliputianNagalasemolecules.BigMacwantstogetupandgooutandkicksomebutt,
butitcant.Immuneactivitygrindstoahalt.Dr.Yamamotoputsitthisway:Macrophagesarethemajor
phagocyticandantigenpresentingcells.Becausemacrophageactivationforphagocytosisandantigen
presentationtoBandTlymphocytesisthefirst(and)indispensablestepinthedevelopmentofbothhumoraland
cellularimmunity,lackofmacrophageactivationleadstoimmunosuppression.(ImmunotherapyofProstate
CancerwithGcMAF.Yamamotoetal.,TranslationalOncologyVol.1,No.2,2008.)
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The GcMAF Book
Chapter8
How Your Body Makes GcMAF

AnillustrateddescriptionofthebiochemicaltransformationsinvolvedinthesynthesisofGcMAFfrom
VitaminDBindingprotein.
HowyourbodymakestheGcMAFthatactivatesmacrophagesandprotectsyoufromcancerand
viruses
GcMAFandNagalasearebothproteins,soletmestartwithabriefandhopefullypainlessprimeronproteins.
Youknowthosebirthdaypresentbowsmadeofclustersofcurlyribbons?Underaverypowerfulmicroscope,
proteinslooklikethat.Theribbonsarelongchainsofhundredsofaminoacidsthatmakeupaproteinmolecule.
OurDNAisprogrammedtomaketensofthousandsofdifferentproteins,andwhatmakesthemdifferentisthe
orderingoftheaminoacids.Eachstrand(usuallytherearethreeorfourofthem)ofcurledribboninourbirthday
bowisoneofthosechains.Thecurlyribbonsareallattachedtogetherwherethebowisfastenedtothepresent.
Theymaylooklikeabigblobofrandomlyplacedbandsandintheribbon,theyare.Butinaprotein,thereisa
veryspecificthreedimensionalstructure,suchthateventhoughthecurlyribbonslookrandomlyplaced,they
are,infact,verypreciselypositionedandevenslightpositionalchangeswillsignificantlyalterthenatureofthe
protein.
VitaminDbindingprotein(DBP)istheprecursorproteinoutofwhichourimmunecellsmakeGcMAF.Upclose
DBPlookskindoflikeasmallBrillopad,buttheconvolutionsarenotsharpedgedtheyreactuallyquitesoftand
sticky.DBPcontains458aminoacids,oneofwhichisveryspecialandquitedifferentfromalltheothers.Thisisa
threonineaminoacid,the420thaminoacidinitschain.Attachedtothisthreonineisagroupofthreesugars.
ThepresenceofthesesugarsdefinesthepurposeoftheentireDBPproteinmolecule.Tokeepthingssimple,Iam
goingtonamethethreesugarmoleculesaftercandybars.
BecauseVitaminDBindingProteincomeswithsugarsattached,wecannowrefertoitasaglycoprotein.Mostof
theimmunesystemsmessengermoleculesareglycoproteins.
NowimagineDBPasthislargeproteinwiththreesugars(orcandybars)attached.ThefirstisaHersheysbar,the
secondisaMilkyWay,andthethirdisaSnickers.Allthreeareattachedtooneanother,asshowninthediagram,
inanupsidedownYshapedconfiguration.
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VitaminDBindingProtein(DBP)isthestartingpointinGcMAFproduction.
DBPistheproteinfromwhichwearegoingtomakeGcMAF.
Thedashes()indicatechemicalbonds(pairsofelectronsthatholdatomstogethertoformchemicals)that
attachthesugarstoeachotherandtotheprotein.
MakingGcMAFfromDBP
NowletstransformourcandybarmodelofDBPintoGcMAF.Therearetwostepsinthisprocess.Thefirststepis
tosnipofftheMilkyWaybar.(Thisisperformedbytheenzymebetagalactosidasewhichisembeddedinthe
outercellmembraneofBlymphocytes.)Youcangoaheadandeatitwewontneeditanymore.Ifyoudont
wantit,yourbodywilljustrecycleit.
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IntermediateinGcMAFproduction.
ThesecondstepistosnipofftheSnickersbar.(Thisisperformedbytheenzymesialidase,whichislocatedinthe
outermembraneofTlymphocytecells.)(Youcanhavethatonetoo,ifyouwanttogetonthefasttrackto
diabetes.)
NowwereleftwithahugeproteinthathasjusttheremainingHersheyshangingoffofit.Guesswhat:thisis
GcMAF.
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Bysnippingofftwoofthethreesugars(firsttheMilkyWayandthentheSnickersbar),wehavetransformedthe
VitaminDBindingProteinintoGcMAF.
Itsfullyformedandreadytofloatoff,findamacrophage,lockontoitsreceptor,andthensendapowerful
messagetotheentirecell,tellingittostopwatchingrerunsofDesperateHousewivesandgettoworkbeatingup
microbesandkillingcancercells.And,asyouknow,whenGcMAFtalks,macroslisten.
Candybaridentitiesrevealed
Justfortherecord(andforyoubiochemistsinthehouse)myHersheysbarisalphaNacetylgalactosamine
(GalNAc),theMilkyWayisDgalactose,andtheSnickersbarissialicacid(alsoknownasNacetylneuraminicacid).
NowthatwehavesynthesizedsomeGcMAF,wellseeinChapter9howNagalasesabotagesit
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The GcMAF Book
Chapter9
Nagalase: Friend and Foe?

WhatisNagalase?
Nagalaseisaproteinmadebyallcancercellsandviruses(HIV,hepatitisB,hepatitisC,influenza,herpes,Epstein
Barrvirus,andothers).Itsformal,officialchemicalnameisalphaNacetylgalactosaminidase,butthisissucha
tonguetwistingmouthfulofamonikerthatweusuallyjustcallitNagalase.(Sometimes,whenIwantto
impressfriendswithmybrilliance,Illsaytheentirewordrealfast:alphaNacetylgalactosaminidase.Ihave
foundthatitsimportanttopracticebeforehandifonedoesntwanttoembarrassoneself.)
WhyisNagalaseimportant?
1. Nagalasecausesimmunodeficiency.NagalaseblocksproductionofGcMAF,thuspreventingtheimmune
systemfromdoingitsjob.Withoutanactiveimmunesystem,cancerandviralinfectionscangrow
unchecked.
2. Asanextremelysensitivemarkerforallcancers,Nagalaseprovidesapowerfulsystemforearlydetection.
3. SerialNagalasetestingprovidesareliableandaccuratemethodfortrackingtheresultsofanytherapeutic
regimenforcancer,AIDS,orotherchronicviralinfection.
Nagalaseprovesthatcancercellsbreakalltherules
Normalhealthycellscooperatewithoneanotherinaconcertedefforttofurtherthegoodofall.Cancercells
refusetoplayball.Theirdisdainfulattitudetowardtherestofourcellularcommunityisappalling.Forexample,
thesecellularscofflawsignoreclearmessagestostopgrowingandspreadingandencroachingontheirneighbors
space.Howwouldyoulikeitifyourneighbormovedhisfenceoverintoyourbackyard?
Ofalltherulescancercellsbreak,noneismorealarmingthantheproductionofNagalase,theevilenzymethat
completelyhogtiestheimmunesystemarmysabilitytostopcancercells.
VirusparticlesalsomakeNagalase.Theirgoalisthesameasthatofthecancercells:survivalbyincapacitating
theirnumberoneenemy:theimmunesystem.
Nagalaseprecision
Likeastealthbomber,theNagalaseenzymesynthesizedinandreleasedfromacancercelloravirusparticle
pinpointstheGcMAFproductionfacilitiesonthesurfaceofyourTandBlymphocytesandthenwipesthemout
withanincrediblyprecisebomb.Howprecise?Letmeputitthisway:Nagalaselocatesandattacksonespecific
twoelectronbondlocatedat,andonlyat,the420thaminoacidpositiononahugeproteinmolecule(DBP),oneof
tensofthousandsofproteins,eachcontainingmillionsofelectrons.Thisislikeselectivelytakingoutaparkbench
inamajorcityfromsixthousandmilesaway.Moreastonishing,ifthatispossible,Nagalasenevermissesits
target.Thereisnocollateraldamage.
Asyoualreadyknow,GcMAFisacellsignalingglycoproteinthattalkstomacrophages,enablingthemtorapidly
find,attack,andkillvirusesandcancercells.Byactivatingmacrophages,GcMAFtriggersacascadethatactivates
theentireimmunesystem.BlockageofGcMAFproductionbyNagalasebringsallthiswonderfulanticancerand
antiviralimmuneactivitytoascreechinghalt,allowingcancerandinfectionstospread.
WhatdoesNagalaseactuallydo?Howdoesitdestroyimmunefunctioninganddeactivate
macrophages?
Oncesynthesizedandreleasedintonearbytissueorintothebloodstream,Nagalase,likethatdrillsergeantat
bootcamp,shoutsharshcommandsatthevitaminDbindingprotein(DBP)thatisabouttobeturnedinto
GcMAF.NagalasedemandsthatDBPnot,underanycircumstances,attachitselftoaspecificsugarmolecule
(galactosamine).IfDBPhasalreadygrabbed(i.e.,connectedto,usingatwoelectron,covalentbond)a
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galactosaminesugarmolecule,itiscommandedtoimmediatelyletgo.Leavegalactosaminealone,oryoullbein
bigtrouble!!!istheNagalasesergeantscommand.Wellprobablyneverknowwhetherornot,onsomedeeper
level,DBPknowsthatNagalasesmotivesaredastardlybutitdoesntreallymatter:DBPwilldefinitelyalways
obey.Likethearmyprivate,theDBPliterallyhasnochoice.Becauseofthewayhierarchiesworkincellular
biology,proteinsmustdothebiddingoftheirenzymes.Theenzymes,likeNagalase,arethedrillsergeantandthe
proteins,likeDBP,aretheprivates.Thatsjustthewayitis.ObeyingthedrillsergeantscommandmeansDBP
cantdoitsassignedtask,thatofbecomingGcMAF.Itisrendereduseless.ForDBP,onamolecularlevel,lifeno
longerhasmeaning.
Unfortunatelyforcancerandviralpatients,DBPhadbeenonitswaytobecomingGcMAFuntiltheNagalasedrill
sergeantsorudelyinterrupted.NowGcMAFtheoneproteinourbodiesneedinordertoactivateourimmune
systemscantbemade.Immuneactivityscreechestoahalt.Thedefensesystemprotectingusfromcancers
andviruseshasbeensnuffedout.
Nagalase,usingthisastonishinglysimpleyetcunninglysubversivetechnique,emasculatestheGcMAFprecursor
protein(DBP)byknockingoffitsthreesugarmolecules.OnequickwhackbyNagalaseandtheDBPproteinthat
wouldhavebecomeaGcMAFmoleculenowlimpsoffintothesunset,permanentlydisfiguredanddisabled.With
onesimple,swiftmaneuver,Nagalasehasbroughttheentireimmunesystemtoitsknees.
HereshowDr.Yamamotoputit(forclarity,Ivereplacedsomeofthetechnicalwords):
SerumvitaminD3bindingprotein(DBP)istheprecursorfortheprincipalmacrophageactivatingfactor(GcMAF).
TheprecursoractivityofserumDBPwasreducedThesepatientseracontainedalphaNacetylgalactosaminidase
(Nagalase)thatdeglycosylates(removesthesugarsfrom)DBP.DeglycosylatedDBPcannotbeconvertedto
GcMAF,thusitlosestheGcMAFprecursoractivity,leadingtoimmunosuppression.(MicrobesInfect.2005
Apr7(4):67481.Epub2005Mar22.PathogenicsignificanceofalphaNacetylgalactosaminidaseactivityfoundin
thehemagglutininofinfluenzavirus.YamamotoN,UradeM.)
Nagalasetesting:formermassmurderernowworksforthegoodguys
ItseasytobealittleschizyaboutNagalase.Ontheonehand,thisnastyproteinsbehaviortowardushasbeen
reprehensibleanddisastrous.WorkingincahootswithcancerandHIVnotshyaboutgettingintobedwithour
mortalenemiesNagalasecanrightfullyclaimdirectresponsibilityforbillionsofhumandeaths.Anditwouldjust
assoonaddyoutothelist,sowedonthavetobeshyaboutplacingNagalaseinthegenocidalmurderer
column.
WiththeadventofNagalasetesting,however,thisbadactornowwillbeharnessedtoausefulpurpose.By
providinguswithpreciseandreliableadvanceinformationaboutenemyoperations,Nagalasebloodleveltesting
becomesaDeepThroatdoubleagentforcancer.Hehelpsusbygivingusanearlywarningsign.
Earlydetection(usingAMASorNagalase)saveslives
Youdontwantacancertohavegottenoutofcontrolbythetimeyoufindandstarttreatingit.Whencancersare
stillyoungandsmall,gentlenaturaltherapiesarethemosteffective.Alternativetreatmentsworkbestonearly
smallcancersbyenhancingimmunefunctioningandremovingthesourceoftheinflammationthatiscausingthe
cancerinthefirstplace.Cancersthathavebecomelargeenoughtoseeonimagingposeamuchmoresignificant
threat,andthebiggunsnowbecomenecessary.
Thecurrentmethodfordiagnosingmostcancersrequiresustowaituntilamassshowsuponimaging(e.g.,a
mammogram,chestXray,orcolonoscopy).Thisapproachwastesvaluabletimeandcausesneedlessdeaths.But
longbeforeimagingcanfindit,apositiveNagalase(orAMAStest)cantellusthatearlystagecancerexists
somewhereinthebody.Byenablingearlierandthereforelessinvasivetreatmentoptions,thisinformation
providesahugeheadstart.
Normallypresentatonlytracelevels,Nagalaseshowsupinthebloodwhenacancerorvirus
appears
ThemalignantandviralentitiesthatmakeNagalasearenotnormallypresent,soitsappearanceisabigdealfrom
adiagnosticperspective.WhenNagalaseshowsup,eveninverysmallamounts,wehavetheearliestglimpseofa
newcancerorviralinfection.Theoldadage,Wheretheressmoke,theresfireapplieshere.ApositiveNagalase
testnotifiesusthatacancer(oranastyvirus)lurkswithin.
Nagalaseappearsinthebloodstreamwhenanascentcancerisjustaminuteclusterofabnormalcells,long
beforeconventionaldiagnosticmethodscandetectit.Throughbloodtesting,wecanfindthisredflag,evenwhen
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presentatexceedinglylowlevels.ProvidinguswiththisearlywarningsignmightnotquitequalifyNagalasefor
theGoodSamaritanaward,butIcouldgowithextremelyuseful.Likearehabilitatedcriminalonparole,the
potentialforharmisstillthere.Fornow,however,hesstayingoutoftroubleanddoingcommunityservice.Turn
yourbackandhesamassmurdereragain.
UsingNagalasetestingtotrackcancertreatment
RisingNagalaselevelsindicateacancerorvirusisgrowingandspreading.Conversely,Nagalaselevelswill
decreaseifthecancerorinfectionisbeingeffectivelydestroyed.
Anytreatmentthatlowerscancercell(orviralnumbers)willlowerNagalaselevels.Nagalasewill,forexample,
alwaysdropaftersurgery(whetherornottheentiretumorwasremoved).Chemotherapyandradiationalso
reduceNagalaselevels.SodoesGcMAF.If,afterthesetreatments,thedepressedlevelbeginstoriseagain,thisis
thewarningsignthatthecancerwasnotcompletelyremoved,and/orthatmetastaticdiseaseishidingout
somewhere.Withviralinfections,increasingNagalaselevelsindicatereturnoftheinfection.
ConsecutiverisingNagalaselevelsarethereforearedflag,warningusitmaybetimetoentertainnewtreatment
options.Conversely,iflevelsaregoingdown,staythecourse:thecancerorvirusisgoingaway.
Flatearthmedicine
ManymedicalprofessionalsdontfeelcomfortablewithnonspecifictestslikeNagalase.Itdrivesthemnutsto
discoverthatacancerislurkingsomewhereinsidewithoutknowingexactlywhereitislocated.How,theyask,
doyouexpectmetotreatacancerIcantsee?Why,Imnotgoingtotiltatwindmills!Thismaybeasignal
thatyouneedtofindadifferentdoctor,perhapsonewhoworksinanalternativecancerclinic.Hereyouwillfind
highlytrainedprofessionalswhounderstandtheconceptthatcancerisamolecularbiologicalchangelongbeforeit
presentsvisually(bythisImeanbecomesviewableonimaging).
WhenGcMAFbecomesavailable,theanswerwillbeeasier:asixmonthcourseofweekly100ngGcMAF
intramuscularinjectionswithmonthlyNagalaselevelteststofollowtheNagalaselevelasitgoesbackdownto
baseline.Thecancercanbedeclaredcured,eventhoughitneverreachedlifethreateningproportions.(Wehavea
longwaytogobeforethiskindofmedicalbehaviorwillbecommonplaceandacceptable.Thesoonerthebetter,
however.)
Nagalaseroleunderappreciated
Nagalase,arguablyourmostimmunosuppressiveproteinmolecule,posesanenormousthreatintermsofcancer
perpetuationandvirusesabilitytocontinuallydefeatus.Yetcancerresearchershavenotshownanyinterestin
it.(MaybeImbeingalittletoogeneroushereperhapscluelesswouldbemoreamoreaccuratedepiction.)Why
donttheygetitthatblastingcancercellsintooblivionwithchemoandradiationisusuallynotsufficienttostop
advanceddiseaseanddoesnothingtoaddressthecause:immunosuppression.Evenifweignoreforthemoment
theexcessivecollateraldamagecausedbychemodrugsandradiation,thepatientalsoneedsrequiresahealthy
immunesystemtofinishthejob.IfwedontreviveimmunefunctionbydisablingNagalase,thecancersand
viruseswilljustkeeproaringback.RestoringimmunocompetencebynegatingthestultifyingeffectofNagalase
shouldthereforebecomeaprimaryresearchgoal.
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The GcMAF Book
Chapter10
How Nagalase Blocks GcMAF Production

CancercellsandallvirusesmanufactureandreleaseNagalase,theenzymethatsabotagesGcMAF.Without
GcMAF,macrophagesremaininadeepslumber.Thischapteranswersthequestion:HowdoesNagalasedoit?
Nagalasesabotage
Asyouknowbynow(becauseIhavereiterateditadnauseum),theNagalasespewedoutbycancercellsand
virusparticlesneutralizestheimmuneactivatingeffectsofGcMAF.FormillionsofyearsGcMAF(andourimmune
systems)havehadnodefenseagainstNagalase.ThenProfessorNobutoYamamotocamealongandfiguredout
howNagalaseparalyzesimmunefunctioning.HethenwentontodemonstratethatreplacingtheGcMAF
deficiencydramaticallyrestoresimmunefunction,effectivelycircumventingtheroadblocksetupbyNagalase.
TheultraminiatureNagalasepowerplaythatImabouttodescribehasleveragefarbeyonditstriflingsize.This
relativelysimplemolecularbiologicalenzymaticreactionhascostbillionsofhumanstheirlives.Whowouldve
thoughtthatchippingafewsugarsoffofaglycoproteinmoleculecouldhavewreakedsuchcarnage?
Asaboteurisonewhointentionallycausesthedestructionofpropertyinordertohindertheeffortsofhis/her
enemy.Thewordderivesfrom15thcenturyNetherlands,where,fearingthatautomatedmachineswouldrender
theiroccupationsobsolete,Dutchworkersthrewtheirsabots(woodenshoes)intothegearsofthetextileloomsin
anattempttobreakthecogs.Likewise,Nagalase,thesaboteur,throwsabootintothebiochemicalmachinery
thatproducesGcMAF.
Liketakingcandyfromababy
NowIwilldescribeonamolecularblowbyblowbasishowNagalaseblocksGcMAFproduction.Recallfrom
Chapter8:HowYourBodyMakesGcMAFthatourlymphocytesmakeGcMAFbyenzymaticallyremovingtwoof
threesugarsfromaVitaminDBindingProtein(DBP)molecule:
VitaminDBindingProteinisconvertedtoGcMAF.
ToappreciatehowNagalaseinterfereswithGcMAFproduction,imaginethatDBPisthebabythatisabouttobe
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blockedfrombecomingGcMAF.BabyGcMAF(AKADBP)istryingtoholdontoitsHersheysbar(AKAN
acetylgalactosamine).Nagalasecomesalongandsnatchesallthreesugarsawayfromthebaby.(Seeillustration
below.)Thesugarsfloataway,offintothecellularsoup,nevertobeheardfromagain.(Oh,Iimaginesooneror
lateraswithothersugarstheyllbeburnedupinamitochondrialfactorytogeneratealittlecellularenergy
butasfarasDBPisconcerned,theyreGone.)
NagalaseblocksGcMAFproductionbydeglycosylatingVitaminDBindingProtein.
Whenthedustsettles,wearealsoleftwithaworthlessdeglycosylatedDBPmolecule.Withallofitssugars
removedbytheNagalase,theresnowayDBPcanbecomeGcMAF.ThankstothesaboteurNagalase,allthat
remainsisauseless,sugarfreeproteinthatcantbeconvertedintoanythingofvalue.Ittoodriftsoffintocellular
obscurityandeventuallygetsrecycled.
Initsunderhandedefforttosubverttheimmunearmy,Nagalasehasliterallystoopedtosnatchingcandyfroma
baby.Thefetus(DBP)thatwouldhavebecomeGcMAFhasdiedinutero.And,Voila!Thepathogenhasneutralized
itsenemysimmunesystem.ThecancercellorvirusthatmadetheNagalasehassucceededinneutralizingits
archenemy,themacrophage.And(aspointedoutearlier,butbearsrepeating),whenmacrophagesarede
activated,cellsignalingissimultaneouslydeactivated,sootherimmunecells(primarilyBandTlymphocytes)
stopfunctioningtoo.Anticancerandantimicrobialimmuneactivityhavebeeneffectivelydisabled.
Nagalaseisremarkablyefficient
BecauseitisanenzymeacatalystNagalaseperformsthismaliciousritualoverandoverandoveragain,and
eachtimeitcomesawayunscathedandunchanged.OneNagalasemoleculecanthusdestroyahugequantityof
GcMAFprecursormolecules.
ToappreciatehowpreciselyNagalase(AlphaNacetylglucosaminidase)targetsGcMAFproduction,imaginea
Stingerheatseekinggroundtoairmissiletrackingafighterjet.Evasivemaneuversevenbythebestpilots
wontoutsmarttheStinger,whichrapidlyadjustsitscoursetotracktheplanedownandblowitup.Nagalase
worksthesameway:ittracksdownandthenpulverizesthe(DBP)precursorsofGcMAFmolecules.
Nagalasehasnonaturalenemies.Nobodilyprocess,nodrug,notreatmentcouldoutsmartthisdiabolicalkiller.
Sure,highpowereddrugsandradiationwilltakeoutmanyofthecancercellsandinsomecasesproduceacure,
butuntilDr.NobutoYamamotocamealongandoutedNagalase,wehadnoideaastotheactualcauseofthe
immuneshutdownthatletcancersandvirusesgowild.
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Dr.YamamotosschematicdiagramsfortheproductionofGcMAF.FIG.1A:TheformationofGcMAFfromGc
protein(vitaminDbindingprotein.FIG.1B:ThedeglycosylationofGcproteinbyNagalase(alphaN
acetylgalactosaminidase).
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The GcMAF Book
Chapter11
If Cancer Cells Could Talk...

Wistfulruminationsofacancercell
Foralongtimewecancercellshadagreatjob.OurNagalaseenzymeStealthbombersquadronsmadeiteasyto
keepcancergoing.SneakinginundertheradaranddemolishingGcMAFproductionfacilitieswasourspecialty,and
ifIsaysomyselfweweregoodatit.InevermissedmytargetandInevercausedanycollateraldamage.We
shutthoseGcMAFfactoriesdownbigtime.Blastedthemintooblivion.Andwehadbillionsofcancerkills.
WithouttheirdailydoseofGcMAF,thosemacrophagescouldntgetoutofbed.Theydidntreallyhaveachance
againstus.Itwasaromp!Cancerruled!
Imsurprisedathowlongittookthescientiststounravelourscheme.Ourkillingspreelastedhundredsofyears!
Tryandmatchthat!!!Liveslostinwarspalesincomparison.Infact,wekilledmanytimesmorehumansthanall
thewarsinhistorycombined.
Evenwhenhumanmolecularbiologistsfinallyfiguredouthowweoperatecrackedthecode,sotospeakand
Dr.Yamamotooutedusbyexplainingtotheworldhowwedidit,itstilltookthemanotherdecadetopay
attentionandfinallyshutusdown.Thecancerestablishmentdidntwanttosacrificetheircashcowso,insteadof
implementingthesenewdiscoveries,theystilljustkeptlobbingthosesillyuselessdrugsatus.Asusual,that
slowedusdownalittle,butittookanothertenyearsbeforetheyfinallystartedroutineNagalasescreening,and
thengivingweeklyGcMAFtoeveryonewithanelevatedNagalase.Whydidittakethemsolongtoseethatfixing
theimmunesystemwasinfinitelypreferabletotheirmostlyfutileattemptstoshutthebarndoorafterthe
horseshadgottenout,sotospeak.Givenhalfachance,thebodycancureitself.Becauseofthatmistakeand
itwasahugeonewekilledmillionsmorebeforetheysidelinedus.
SohowdidIpersonallyescapetheGcMAFandactivatedmacros?Well,Imoneoftheluckyones,andImustsay
thereareveryfewofus.WhenDr.YamamotodiscoveredNagalase,Iswitchedsidesandchangedmyidentity.
NowIminasortofwitnessprotectionprogram.TheonlyreasontheydontkillmeisbecauseImusefulto
themasamole,awhistleblowertheycallitacancermarkerhoweveryouwanttoputit.Thedocstestlevels
ofmetoseewheteher(andhowmuch)cancerorvirusispresentinmyhostsbody.
Imafortunateguy.BeforeGcMAF,thecancercellcommunityadoredusNagalasemolecules.Now,postGcMAF,
Iamstillahero,buttotheotherside.NowIhelpdoctorsfindnewcancersandtrackknownones,sosuddenlyI
wentfrombadguytogoodguy.Now,asaspy,Iprovideyouguys,myformerenemies,withearlywarningsthat
cancerisonthemove.AndIletyouknowwhetheryourtreatmentsareworking.Hereshowthatworks:Moreof
memeansmorecancer(orvirus),forsure.Andlessofmemeansthecancers(orvirus)aregoingaway.IfI
appeartobegoingawaybutthencomeback,youllknowthecancerorHIVhasreturned(i.e.,wasntfullykilled
off)andmyownerpersonneedsanotherroundofGcMAF.
Itsajob,andsomebodyhadtodoit.Consideringthealternatives,welllikeIsaidImaluckyguy!
LookingatNagalasefromafutureperspective:BackinTheDay
Itis2020.Twocancercells,JimmyandJack,arehavingachat.JimmystheyounggogetterandJackthe
seasonedbutterminallycynicalveteran.
Jack,inanimatedtones,reminiscesaboutthegoodolddayswhencancercouldwreakhavoc
IwanttotellyouaboutMickey,thelegendarycancercellwhodiscoveredNagalase.Mickeysdiscoverycatapulted
uscancercellsfromaminornuisanceupintooneofthealltimegreatestthreatstothesurvivalofhumanity.Of
course,thosedaysareovernow,butman,wehadquitearun.Millionsofyearsandbillionsofkills!Notbad,huh?
Oh,therevebeenotherfamouscancers.HenriettaLacksimmortalcervicalcancercelllinecomestomind.But
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eventhoughheslesswellknown,Mickeywasbyfarthegreatest.WithMickeysNagalaseinourarsenal,wewere
unstoppable.LikeBruceWillisorTheTerminator.Theoncologistsandsurgeonsandradiologistscouldaimtheir
peashootersatusalldaylongthechemo,thesurgery,theradiationbut,man,wejustshruggedthemoff
andkeptoncomin.
Butitsallchangednow.Right,Jack?Imean,theyretestingjustabouteverybodyforNagalasenow.Wedont
haveachance.Jimmytentativelyintones.Heknowsastoryiscoming.
MickeydiscoveredNagalasebackintheancienthistory,Jimmy,inthestoneageofcancer.Beforewritten
history,sowedontknowexactlywhen.Andhowhedidit,Idontknowinfactnobodyknows.Probablya
mutationinhisgenomethatgeneratedaproteinthatstoppedcancerinitstracks.OfcourseJimmyscontribution
anditsnotonetobesneeredatwasrecognizingitsvalue.Alessercancercellmighthavereceivedthegiftof
Nagalaseproduction,butfailedtoappreciateitscrucialimportancebutnotMickey.HesawthatNagalasestopped
themacrosintheirtracks
Howdoesitwork,Jack?
Itssosimple.Nagalasesimplybreaksapairofelectronsapart.Thisparticularpairofelectronsmakeupabond
thatholdsacrucialpartoftheGcMAFprecursormolecule(themoleculeGcMAFismadeoutof)together,sothe
GcMAFcantbemanufactured.Aalakazam!!!NomoreGcMAF!Cantbemadebecausetheprecursordisintegrated.
Bottomline:nomoremacroactivation,nomoremacroseatingmypals.Endofstory.Wewin.
Wow,Jack.Thatsamazing.
AndMickeyalsofiguredouthowtospreadthewordandmakealotmoreNagalase.
How?
Byteachingallhisfriendsandneighbors,ourdistantrelatives,howtomakeit.Theyjustsharedthegenesforit.
Nobigdeal.Afterawhile,duetoDarwiniansurvivalofthefittest,allofourcancercellbrethrenthatcouldnt
makeNagalasejustdiedoff.Maytheyrestinpeace.Well,actually,theydidntexactlydietheywereeatenby
themacros.ButtheonesthatcouldmakeNagalasesurvivedandpassedonthegene.Weusethehumansto
carryitaroundintheirDNA.Thepowertosetusfree(andmakeusdominant)remainedinthere,lockedinto
perpetualexistenceinthehumangenome,theblueprintforlifeanddeath.Itwasreplicatedandpassedonfrom
unsuspectinggenerationtounsuspectinggenerationinsidethehumans,anditjustsitsthereinalloftheir
genomes,lyinginwait,readytobeexploitedbyuscancercellswheneverwegetthechancetostartgrowing.And
bysmoking,eatingpesticidesandspewinganenormousarrayoftoxicchemicalsintotheenvironment,theygave
usplentyofopportunities.
Then,backin2010,alongcameGcMAF.ThedocsfinallyfiguredoutthatiftheygavepeopleGcMAF,itwould
bypasstheNagalaseandactivatethemacros.Now,insteadofrulingtheroost,weareontherun.The
macrophagesareeverywhere,buzzingaround,ganginguponus,andtheresnowheretohidelikeintheolddays.
Theycantakeoutbrigadesofourcellswithnoeffortatall.Andtheytargettheyoungunsthatsnotnice!
Ifyouaskme,GcMAFiscruelandunusualpunishmenttouscancercells.Wewerejustdoinourthingman,if
youknowwhatImean.
Butthosewerethegoodolddays,Jack.Nowitsdifferentandwehavetoadjust
Youreright,Jimmy,agoodthingcantlastforever.Wehavetoacceptthatourrunisover,ourtimehaspassed.
Nagalasetestinghasbecomemorepopularthancholesterolusedtobe.Nowadays,everybodygetstestedfor
Nagalase,startingcanyoubelieveit,inhighschool?Theyfindussoearlynowandthentheypouronthe
GcMAF.Wedonthaveachanceanymore.Usuallythemacrosnailussoearlythatwecantevenmetastasize.
Addinginsulttoinjury,thentheymonitoruswithNagalasetestinguntiltheyresureweregone.Normally,we
cantgetaserioustumorgoing.Ourcurrenthost,howeverman,thisdudeisclueless.Forsomereasonor
other,hehasntgottentested.Thatstheonlyreasonwerealivehere,Jimmy.
Oh,westillhavepollution,toxicityandchemicalsonourside,sowellbehangingaroundinsmallnumbers,
generatingtheoccasionalnewmalignantcolony,butunlesswecomeupwithsomethingreallygoodlikeNagalase
usedtobe,wearebasicallytoast.Itsonlyamatteroftime.
Then,startled,Jimmyscreams:Oh,oh.Yikes!!!!Whatsthathugeblobcomingaroundthecornerofthebuilding
overthere?IsthatwhatIthinkitis?
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Dang!Yup.Thatsamacrophage,son.Well,Ifiguredtheydcomeafterussoonerorlater.Thisguymusthave
finallygottentested.Weredeadmeatnow,Jimmy.Itscomingtoeatus!!!Dodgethosesuperoxideradicals,if
youcan!Watchoutforthatpseudopod!Itsbeenniceknowingyou,Jimmy.Arggggh.
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The GcMAF Book
Chapter12
GcMAF and HIV/AIDS

Dr.Yamamototreated15HIVpatientswithGcMAF,whicheradicatedthevirusinallcases.These
patientswentintoremissionandremaineddiseasefreeduring7yearsoffollowup.
Tosabotagetheimmunesystemandputmacrophagestosleep,allvirusesmakeNagalase,theenzymethat
blocksproductionofGcMAF.WithoutGcMAF(theproteinthatactivatesmacrophagesandjumpstartstheentire
immuneresponse)HIVandothervirusescangrowunimpeded.Nagalaseputstheimmunesystemtosleep.Dr.
NobutoYamamotodemonstratedthatGcMAFadministrationbypassestheNagalaseblockageandreactivatesthe
macrophages,whichthenproceedtokilltheHIVvirusesandcuretheinfection.
Somethingtocheerabout?
PeopleinfectedwiththeHumanImmunodeficiencyVirus(HIV)havesomethingtogetexcitedabout.Theyjust
dontknowityet.In2009Dr.NobutoYamamotopublishedalandmarkpaperentitled:ImmunotherapyofHIV
InfectedPatientsWithGcProteinDerivedMacrophageActivatingFactor(GcMAF)intheJournalofMedical
VirologyinwhichhedemonstratedthatGcMAFcured100%ofnonanemicHIVinfectedpatients.Afterseven
yearsoffollowup,therewerenorecurrences.AllpatientsmaintainedhealthyCD+counts.
Ofcourse,thisisjustonestudy.Andithadthedisadvantageofcontainingsomecomplexmolecularbiological
chemospeak.IfthereaderwerentfamiliarwithYamamotosdecadesofbackgroundresearch(allofwhichwas
publishedinjournalsthatHIVresearchersandpatientswouldntbelikelytoread),thisstudywouldfallondeaf
ears.ButProfessorYamamotosHIVstudywasnoquirk.Basedonaquartercenturyofsolidresearchthat
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predictedsuccesslongbeforetheactualhumantrials,itpresentedallthescienceonewouldneedtounderstand
exactlywhytheseHIVpatientswerecured.
Thoughthisstudywaspublishedin2009,therehasbeennoinformeddiscussiononthistopic,nofurtherGcMAF
researchasatherapyforHIV,andnomediachatter.Itsasifthisstudyneverhappened.Whyisthis?
HowGcMAFdestroysHIV
HIVlikeallvirusesmakesNagalase,theenzymethatblocksGcMAFproduction.WithoutGcMAF,macrophages
becomeindolentandtheantiviralimmuneresponseshutsdown.ThisallowstheHIVinfectiontospread.To
remedythissituation,Dr.YamamotosimplygavethesepatientsGcMAF.Thisreactivatedthesleeping
macrophages,whichthenproceededtophagocytizealloftheviruses.
TheprecisemolecularbiologicalpathwaysandmechanismsinvolvedwithHIV,Nagalase,andGcMAFareidentical
tothoseforcancercells,andneednotberepeatedhere.
InhisHIVstudyYamamotofirstshowedthatHIVpatientshadhighNagalaselevelswhichcorrelatedwiththeir
highHIVRNAlevels(awaytomeasuretheamountofHIVinfection).Then,asheadministeredGcMAF(100ng.
onceaweekfor18weeks),allpatientsNagalaselevelsgraduallywentdowntocontrollevels,and,intandem
withtheNagalase,viralloadwentdowntozero.Yamamotowrotethatthesedatasuggestthatthesepatients
werefreeofbothHIVvirionsandHIVinfectedcells.
ProfessorYamamotofollowedthesepatientsforsevenyears,andtheirviralload(HIV1RNA),CD4counts(helper
cells,atypeoflymphocyteusedtoevaluateimmunocompetence),p24antigen(HIVspecificantigen),viral
culture,andNagalaselevelsremainednormal.Allpatientscontinuedtobefreeofdisease.(Note:anemicHIV
patientswereexcludedfromthisstudy.AnemiaiscommoninHIVpatients.TheeffectofGcMAFonanemicHIV
patientsisthusunknown.)
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The GcMAF Book
Chapter13
The AMAS TestAn Alternative To Nagalase Testing

TheAMAStestisusefulbothasascreeningtestforearlycancerandformonitoringcancertherapies.
AMASiselevatedwhencancerispresentandgoesdownbelowbaselinewhencancerisgone.AMAS
isover99%accurate(whendonetwice)andcanbeusedinsteadofNagalasetofindandfollow
cancers.(UnlikeNagalase,AMASdoesnotdetectthepresenceofviruses.)
TheAMAStest:
anaturallyoccurringantibodypresentinserumofallpeople,includingchildren
accuratelydetectsearlycancerofalltypes
positiveifanytypeofcancerexistsanywhereinthebody
greaterthan95%accuracyrepeattestinggreaterthan99%accuratefalsepositiveandfalsenegative
rateslessthan1%
theearliestanticancerantibodytoappear
detectscancerveryearlyyourdoctormaynotbeabletofindthecancer
detectscancerslongbeforetheyappearonimaging
earlydetectiondramaticallyincreasesthepossibilityofapermanentcure
goesdownwithsuccessfulcancertreatment
normallevelsinsuccessfullytreatedcancerpatientsindicatesabsenceofmalignancy
TheAMAScancertest:analternativetoNagalasetesting
UntilNagalasetestingbecomesavailable,IrecommendtheAMAS(AntiMaligninAntibody,Serum),atestthat
definitivelydetermineswhetherornotcancerispresent.Inthisbroadbasedstudy(clickonref13)dozensof
researchersandmedicalcentersconclusivelydemonstratedthevalueofAMASasascreeningtoolforfinding
cancersearly.
Allcancersmakeantimaligninantibody.Becausewearemakingcancercellsallofthetime,antimalignin
antibodiesarepresentatlowlevelsineveryone.Normally,ahealthyimmunesystem(onewithactivated
macrophages)isdestroyingthesecancercellsastheyareformed.AnAMASlevelthatrisesbeyondthebaselineof
135,however,tellsusthattheimmunesystemisnotgettingridofthosenewcancercellsinatimelyway,and
theirnumbersarethereforeincreasing.Cancerisafoot.
AMASisbothacancerscreeningtestandacancermonitoringtest.Inotherwordsyoucanuseittodetermine
whethercancerispresent,andyoucanuseittotracktherapy.
ThebrainchildofneurochemistSamuelBogoch,M.D.,Ph.D.,AMASissimilartothePSAforprostatecancerand
CEAforcolorectalcancer,exceptthatAMASsimultaneouslyscreensforallcancertypes,notjustone.
ApositiveAMASwilltellyouthatcancerispresent,butitwonttellyouwhatkindofcanceritis,anditwonttell
youwherethatcancerislocated.(Notknowingacancersnameandlocationtendstodrivedoctorsnuts.How
canyoutreatacancerwhenyoudontknowwhatitisorwhereitis?theymutter.)
HowAMASworks
Ourimmunesystemrecognizesanantigenicproteinonthesurfaceofcancercells.In1988Dr.Bogochdiscovered
thisantigenandnameditmalignin.Whenourimmunesystemseesmalignin,itstartsmakinganantibody
namedantimaligninantibody.Dr.BogochthendevelopedtheAMAStesttoidentifythepresenceofcancerby
identifyingthepresenceofantimaligninantibody.
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UsingtheAMASformonitoringcancer
Whenatreatmentshrinksacancer,theAMASwillgodown.Whetherthattreatmentissurgery,chemo,
radiation,analternativecancertherapylikeGcMAF,orspontaneousremissionifthecancerissmaller,theAMAS
goesdown.Ifcancerremains,theAMASispositive.Whenacancerisgone(again,regardlessofcause),theAMAS
revertstonormal.
ArisingAMAStellsuscancerisgrowing
AMASisanextremelypreciseimmunoassay.Levelsabovebaselineindicatethepresenceofcancerwith95%
accuracyonthefirsttestingandover99%accuracywhentestedtwice.
ThecutoffpointforapositiveAMASis135.Morethan99%ofpatientswithcancerhaveAMASlevelsabove135.
AMASlevelsbelow135areseeninnormalindividualswhodonothavecancer.
SequentialAMASlevelscanalsobeusedtotracktumoradvancementandtheeffectivenessoftherapies.AMAS
levelswillalwaysincreaseifcancerisgrowingandspreading.Conversely,AMASlevelswilldecreaseifthecanceris
beingeffectivelydestroyed(whetherbysurgery,radiation,chemotherapy,GcMAF,orotheralternativecancer
therapies).
AMASandbreastcancer
AMAShasidentifiedbreastcancersassmallasapencildot(toosmallforanyformofimaging).
ClinicalresearchdatashowsthatbreastcancercanonlybepresumedcurediftheAMASreturnstonormal
(<135)aftertreatmentandthatbreastcancercannotbepresumedtobeinremissionunlessAMASreturnsto
normal.
Theusualfollowupmethodsforbreastcancerincludeimaging(CTscans,MRIs,orxrays)andhormonalblood
tests,lookingforsignsofcanceraftertreatment.AMAStestingprovidesamuchmoreaccuratewaytoknow
whethercancerisstillpresent,andatafractionofthecost.Nottomentiontheinconvenience.
Althoughthesestudiesweredoneonlyonbreastcancerpatients,thereisnoreasontobelievethattheresults
wouldnotapplytoalltypesofcancer.
Cancerpatientswhonolongerhaveevidenceofcanceronimaging,butdohaveapositiveAMAStestcouldnipa
returningcancerinthebudbyutilizingnaturalalternativecancertherapies(includingGcMAFwhenitbecomes
available).
Avoidingunnecessarybiopsies
Ifapatienthasamassonimaging,theAMASwilltellwhetheritismalignantornot.AnegativeAMASmeansthe
massisnotcancerous,andthereforeabiopsyisnotnecessary.UseoftheAMAStestcouldthusreducethepain
andsufferingofneedlessbiopsies.Nottomentionthecost.
AMAScomparedtoNagalase
AMASdiffersfromNagalaseinthatAMASisspecificforcancer,whileNagalaseidentifiesanenzymemadebyboth
virusesandcancercellsandisthereforeunable(byitself)todistinguishbetweenthetwo.BecauseNagalase
testingcannotdistinguishthedifferencebetweencancerandvirus,AMASisabetterscreeningtestforcancer.
HowtoordertheAMAStest
ToorderafreeAMASkit,call18009228378ororderonlineat:http://www.oncolabinc.com.AMASisaproduct
ofOncolab,Inc.,36TheFenway,Boston,MA02215.Phone:6175360850.
Pleasenote
AccordingtoOncolab:TheAMAStestshouldbeusedinthecontextofgoodclinicaljudgmentbyaphysician
experiencedinthetreatmentofcancer.
AnormalAMASlevelcanoccurinnoncancer,interminalcancer,andinsuccessfullytreatedcancerinwhich
thereisnofurtherevidenceofdiseaseclinicalstatusmustbeusedtodistinguishthesestates.
Asinallclinicallaboratorytests,theAMAStestisnotbyitselfdiagnosticofthepresenceorabsenceofdisease,
anditsresultscanonlybeassessedasanaidtodiagnosis,detectionormonitoringofdiseaseinrelationtothe
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history,medicalsignsandsymptomsandtheoverallconditionofthepatient.
Possiblefuturedevelopments
Dr.BogochhasshownthatpurifiedAMASkillscancercellsinatesttube.Fromthisresearchafuturetreatment
canbedevelopedinwhichinjectionsofantimaligninantibodywouldbeusedtotreatcancer.
Dr.BogochscontributiontoscienceandmedicinewillhopefullysomedayberecognizedwithaNobelPrize.
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The GcMAF Book
Chapter14
Biomarker Testing
ThischapterwillshowyouhowtouseAMAStesting,Nagalasetesting,andviralantibodytestingto
determinewhetheryouhavecancer.Ifyoudohavecancer,youlllearnhowtotrackyourtreatment
programtomakesureitisworking.
ItisimportanttoappreciatethefactthatbothcancersandvirusesmakeNagalase.Therefore,apositiveNagalase
testresulttellsusbothcancerand/orviruscouldbepresent,butitdoesnttelluswhich.Thisproblemcanbe
easilysolved.SincetheAMAStestisspecificforcancer(andnotviralinfections),apositiveAMAStellsuscancer
causedtheNagalasetogoup.Viralantibodytestingcanalsobeusedtorulespecificvirusesinorout.Thusthe
combinationofAMASandviralscreeningwilleffectivelysortthroughthepossibilitiespresentedbyapositive
Nagalasetest.
Hereisasummaryofwhateachtestwilltellyou:
ApositiveNagalasetestindicatespresenceofeitheraacancerorviralinfection,orboth.
AMAStesting(nowavailable)indicatespresenceofcancer(only).
Viralantibodytesting(nowavailable)indicatespresenceofspecificviruses(seelistbelow).
Appropriatecombinationsofthesethreetestswillsortthroughthepossibilitiesandidentifytheactualcause
ofthedisease.
UsingNagalasetoscreenforcancer
Inthe(hopefullynottoodistant)future,onceNagalasetestingandGcMAFareavailable,allpeopleatriskof
canceri.e.,everyoneovertheageof40willgetanannualNagalasetestalongwiththeirotherroutineblood
tests(completebloodcount,comprehensivemetabolicpanel,vitaminD,lipidpanel,etc.).
Nagalasewillbecomeourstandardmarkerforearlycancerdetection.ThosewithanelevatedNagalase(after
rulingoutaviralcauseseebelow)willbetreatedpresumptively(i.e.,weknowitsinthereeventhoughwe
cantactuallyseeit)withGcMAFand/orotheralternativecancertherapies.
FollowupNagalasetestingwilldocumentthepatientsprogress.Oncethecancerisgone,asdocumentedbya
returnofNagalasetobaseline,subsequenttestingwillprovideanearlywarningifcancerstartsgrowingagain.
UsingNagalasetotrackeffectivenessofcancertherapy
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Themorecancercellspresentinthebody,themoreNagalasetheygenerate.Thus,Nagalaseistheperfect
markerfordeterminingresponsetocancertherapiesbecause,inagivenpatient,tumorburdenwillalwaysbe
proportionaltotheNagalaselevel.
Incancerpatients,adecliningNagalaselevelreflectsareductionintotaltumorloadorburden.Wewould
expectalowerNagalaseafterchemo,radiation,orsurgerybecausethesetreatmentsallreducetumorburden.
Havingusedthesetherapiesdoesnotmeanthediseaseisgone,however.Averylow(baseline)Nagalaselevel
wouldindicatethecancerhasbeencured.
IfthereisevenaremotechanceofmetastaticdiseaseasindicatedbyanelevatedNagalaselevelitwouldbe
wisetouseGcMAFtoactivatetheimmunesystemsoitcanfindandpolishoffanyremainingcancercells.Repeat
testingwithdeclininglevelswouldindicatethatthetreatmentisworking.
UsingAMAStoscreenforcancer
TheAMAStest(AntiMaligninAntibodyinSerum)measuresserumlevelsofAMA,anantibodyfoundtobe
elevatedin99%ofpatientswithactivemalignancies.
UntilNagalaseisavailable,IrecommendtheAMASasascreeningtestforallpeopleover40.SeeChapter13fora
completedescriptionoftheAMAStest.
UsingAMAStotrackeffectivenessofcancertherapy
TumorsproduceAMASindirectproportiontotheirsize,sodecliningAMASlevelsindicatethecancerisshrinking
andthatthetreatmentprogramisworking.IncreasingAMASlevels,ontheotherhand,indicatecancergrowth
andthatadifferenttreatmentapproachshouldbeconsidered.(AtleastthreeAMAStestsarerequiredtoestablish
apattern.)
UsingAMAStodeterminewhetherapositiveNagalaseindicatespresenceofcancerorvirus.
SinceallvirusesandallcancersmakeNagalase,anelevatedNagalaselevelcouldbecausedbyeither.AMAS
testing,howeverisspecificforcancer(i.e.,tellsusnothingaboutviruses).SoanelevatedNagalaseandanormal
AMASindicateaviralinfection.If,conversely,bothNagalaseandAMASareelevated,youknowyouaredealing
withcancer.
Usingviralantibodytesting
AnegativeAMAScoupledwithapositiveNagalaseindicatesthepresenceofaviralinfection.Antiviralantibody
testingwillidentifythespecificvirus.Eachvirusgeneratesitsownspecificantibodies.Themostcommonchronic
viralinfectionsarelistedbelow.Antibodytestingisavailableforeach:
Herpeszoster
HerpesSimplexI
HerpesSimplexII
EpsteinBarrvirus(mononucleosis)
HepatitisB
HepatitisC
Cytomegalovirus(CMV)
HumanImmunodeficiencyVirus(HIV)
Presumptivetreatmentofoccultcancer
Conventionalmedicinecontinuestoprefertowaituntilimagingrevealsboththepresenceandlocationofacancer
beforeacknowledgingitsexistenceandinstitutingtherapy.Foroccultcancerstheonesthatcantbeseen,
buttestingsaystheyreintheresomewherethebestcourseofactionistotreatpresumptively.Inthis
situation,apositiveNagalaseand/orAMAStestinghastolduscancerispresent,sowepresumethepresenceof
cancer,andthengoaheadandtreatiteventhoughitsstilltoosmalltobeseenonimaging.AnAMAStestdone
twiceandpositivebothtimesidentifiesthepresenceofcancerwith99%accuracy.ApositiveNagalase(oncethis
testbecomesavailable)coupledwithapositiveAMASwouldalsoconstituteconvincingevidenceofanoccult
cancer.
Ifthetreatmentworks,wemayneveractuallyseethecancer.Thismaytroublesomephysicians,butask
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yourselfthisquestion:wouldyouratherremoveasmallcancerwithnatural,harmlessmedicine,andnevergetto
seeitorwouldyouprefertowaituntilthemalignancyenlargestothepointwhereitllshowuponaCATscan,
whichmeansyoullnowneedabiopsy,surgery,radiation,andchemotherapy?Hello?
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Chapter15:EradicatingtheScourgeofCancerfromtheFaceoftheEarth
The GcMAF Book
Chapter15
Eradicating the Scourge of Cancer from the Face of the

Earth
Wecangetridofcanceronceandforall.Wenowpossessthescientificunderstandingnecessaryto
accomplishthis.
Iwanttoabolishcancer.Thisgoalmayseemimpossible,butitsnot.
Earlystagecancersarealoteasiertotreat,socatchingamalignancyearly(whenitis<5mm,toosmalltobe
seenonimaging)isacrucialpieceoftheeradicationstrategy.Bythetimeacancerisbigenoughtobeseenona
CATscan,itstoolatetorelysolelyonnaturalmedicines.Theconventionaloptionshavebecomenecessary:some
combinationofsurgery,radiation,and/orchemotherapy.(Ofcourse,alternativemethodsstillcanandshouldplay
animportantadjunctiverole,butnoresponsiblealternativeoncologistwouldrecommendbypassingtheusual
mainstreamtherapies.)
Sothetricktoreversingcanceristocatchitearly.Thatswhenthegentle,natural,nontoxic,noninvasive,
immunesupportiveoptionsworkbest.ThatswhenGcMAFworksbest.WhenGcMAFbecomesavailable,wehave
everyreasontoexpectthatitseffectivenessinsmallearlycancerswillapproach100%.Wejustneedtofind
them.
How?Nagalasescreening.ElevatedNagalaselevels(repeattestingisnecessarytomakesurethelevelisgoing
up)revealthepresenceofcancerswhentheyarestillextremelysmall.Atthisearlystage,afewmonthsof
weeklyGcMAFinjectionswillbeallthatisneededtoreactivatethemacrophagesandeliminatethecancer.
Applyingthestrategyof1.)annualNagalasetestinginlargepopulationswith2.)weeklyGcMAFtherapyforthose
whotestpositivehasthepotentialtoobliteratethescourgeofcancerfromthefaceoftheearth.
Letsdoit.
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The GcMAF Book
Chapter16
RetroDocs and NanoMedicine: A Rant

ConventionaldoctorsaregoingtohavetoletgooftheirneedtoseeacanceronanXraybefore
treatingit.Inthenanoworldofmolecularmedicinewecannowcontemplateapatientsmetabolic
landscapetospotsubtlebiochemicalmarkerssignpostsalertingustocurrentorfuturedisease.
Severalexamplesareprovided.TestslikeAMAS(now)andNagalase(inthefuture)trackthe
molecularbiologicalfootprintsofmalignancy,enablingdiagnosisandinitiationoftreatmentmuch
earlierintheevolutionofthediseaselongbeforeimagingcoulddetectamass.
Molecularbiology:rewritingtherulesformedicalpractice
Letmetakeabriefdetourheretoaddresstheissueofdoctorswhoareuncomfortabletreatingacancerthatis
toosmalltoseeonimaging.Inordertofeelokayabouttreating,theseguysreallyneedvisualandjustarent
comfortablewithbiochemicalevidence.Inthis,thedawningnewageofmolecularmedicine,however,thezone
betweennodiseaseandclearcut,palpable,imageablediseaseisbecomingincreasinglyblurry.Thanksto
sophisticatedtestingandadeeperunderstandingofthemolecularbiologyofpathologicalprocesses,wearenow
abletoseetheearlieststagesofdiseaseandeventhepreexistingbiochemicallandscapethatsetsthestage
foritlongbeforewecanpindownitslocationwithimaging(Xrays,CATScans,andMRIs).
Myadvicetodoctorswhoneedvisualasopposedtobiochemicallaboratoryevidenceofdiseaseisthis:getover
it!Wehaveenteredthenanoageofmolecularmedicine,wherewecandetecttheearlieststagesofdiseasevia
biochemicalchanges.Intheoldendays(tenyearsago)imagingandpalpatingdiseasewerethebestwehad,but
thesemodalitieshavebecomeincreasinglyarchaicasgeneticsandmolecularbiologicaltestingpavethewayto
earlieridentificationofpathologicalchanges,allowingustoreversediseasewhileitisstillintheformativestages,
beforesymptomsappear.
Asaprimeexampleofthistrend,Nagalasetestingwillbecomethenewcholesterol,thescreeningtestforcancer
thatwillbepartofregularbloodtesting.UsingelevatedNagalaseandAMASlevels,wewillbeabletotreatand
reversetumorswenevergettoactuallyseemuchlessbiopsy.
Viamolecularmedicinetestingwecannowcontemplateapatientsmetaboliclandscapeexaminingsubtle
geneticandbiochemicalmarkersthataresignpostspointingtoacurrentlydevelopingorevenafuturedisease.
Forexample:
AlowDHEAS(anadrenalhormonethemostprevalenthormoneinthehumanbody)level,forexample,tellsus
thispersonisnotcapableofmanagingstressverywell,andthuspronetocancer,allergies,autoimmunedisease,
andotherimmunologicaldisorders.Becauseoftheircompromisedabilitytomanageallkindsofstress(toxic,
inflammatory,infectious,allergic,chemical,traumatic,emotional)individualswithlowDHEAlevelshaveshorter
lifespans.
AnelevatedMCV(meancorpuscularvolumeabove90)onasimpleCBC(completebloodcount)tellsusthe
patienthasavitaminB12and/orfolicaciddeficiency.Untreated,thissignificantlyraisesriskforahostofdiseases
includingcardiovasculardisease(heartattack,stroke,senilebraindisease),neurologicaldiseases(depression,
dementia),andallcancers.
ThevitaminDdeficientindividualisasittingduckforcancer,diabetes,autoimmunedisease,osteoporosis,anda
hostofotherafflictions.VitaminDdeficiencyverycommon(over70%ofAmericanshaveone)andisasuresign
ofdramaticallyhigherriskofneoplasm.Ideallevelis50100.Levelsbelow30putoneat400%baselineriskfor
alltypesofcancer.Testingistheonlywaytodeterminedeficiency.Though10,000IUadaywouldbea
reasonabledoseforsomeonewhoisdeficient(below50),testingisrequiredtodetermineoptimumdose.
ApositiveAMAS(AntiMaligninAntibody,Serum)testtellsuscancerisabsolutelypresent.(SeeChapter13.)
AMASmeasuresantibodiestheimmunesystemmakesinresponsetocancercellantigens.Thistestis95%
accurate99%onrepeat.
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AnelevatedNagalase(enzymemadebycancercellsandvirusesthatprotectsthembyshuttingdownthe
immuneresponse)tellsusacancerorvirusisbrewing.
Iwroteanentirebook(OutsmartingtheNumberOneKiller(http://www.renewalresearch.com/tnok/TNOK
text.pdfandhttp://www.renewalresearch.com/tnok/TNOK.pdf)abouthowtousesixsimplemolecularmedical
markerstopredictatherosclerosis(heartattack,stroke).Oncethebiochemicalaberrationsthatcause
atherosclerosishavebeendetected,naturalmedicinescanbeusedtoreversethem,longbeforesymptomsappear
(suddendeathisthefirstsymptominaboutathirdofallcases)andlongbeforeimagingcouldidentifythe
blockage.Anaddedbonuswouldbethediseaseisdiagnosedbybloodtesting,somoreinvasiveapproaches
(intravenouscoronaryangiogramsarenotmyideaofafuntime)canbesidestepped.
Adoublestandard
Thelipidpanelisareallylousymarkerforcardiovasculardisease.Halfofallpeoplewithanelevatedcholesterol
neverhaveaheartattackorstroke.Halfofpeoplewhogetastrokeorheartattackhaveanormalcholesterol.
Nevertheless,doctorspayliteralhomagetoanelevatedcholesterolbywhippingofftheobligatoryprescriptionfor
astatindrugtreatingitgivesthemthefeelingtheyarenippingsomethinginthebud,eventhoughtheyhave
notaclueaboutwherethatbudmightbelocated.SowhydodoctorsignoreevenfeartheAMASandNagalase
teststhatwoulddothesamethingforcancerthatcholesteroldoesforcardiovasculardisease?Itjustdoesnt
makesense.(Sometimes,inmydarkermoments,Iwonderiftheavailabilityorlackthereofofadrugforthe
abnormalmarkermighthavesomethingtodowithit?)
Earlierdetectionallowsearliertreatment
Treatingabnormallabresults(ratherthanpositiveimagingorphysicalexams)allowsustobegintreatingearlier
andearlierinthediseaseprocess.Asintheexamplesabove,wecandetectandreverseadiseasewithdiet,
exercise,andnutritionalsupplementsifwecatchitearlyenough.Thelongerwewaittogettheoldtime
diagnosis,themoreadvancedthediseaseandthemoreextremethetreatmentmeasuresmustbe.
Intermsofcancer,thebottomlinehereisthatpracticingthebestpossiblemedicinewillforceustodispensewith
theluxuryofpalpatingthemassorseeingitonanXray.Liveswillbelostifwestaystuckinthatgroove.The
bestdoctorswillbeusingnewerbiochemicaltechniquestofinddiseaseearly.
WhenrisingNagalaselevelsexposetheseearlyoccultcancers,thefirstlineoftherapywillbeGcMAF(100
ng/week,intramuscularly),alongwithimmunestrengthening,anticancernutritionalmedicines.IftheGcMAF
programworks,theNagalaselevel(whichshouldbecheckedmonthly)willgodownandthedoctorandpatient
canrestassuredthatthecancerisgoingaway.Wellsleepbetteratnight.
Asmentionedabove,itmayseembizarreandsurrealtobetreatingacancerthatisstillinvisible,butthisis
exactlywhatishappeninganditworks.Dr.YamamotosstudiesshowedthatwithGcMAFitworks100%ofthe
time.
OnceNagalasetestingisavailable(andphysicianshavelearnedhowtouseit),therulesfortreatingcancerwill
change.Nagalasetestingwillnotonlypermitextremelyearlydetectionofthepresenceofcancercells,itllalso
giveusahandleonhowmuchcancerthereis(wecallthisloadorburden).Anditcanaccomplishthese
dauntingtasksbeforethetumorhasexpandedtothepointwhereimagingcanblowitscover.
Guidanceinthisprocessbyatrainedprofessionalisabsolutelycrucial.Tryingtobeyourownplumbermaywork
attimesaleakyfaucetprobablywontkillyoubutinmattersoflifeanddeath,itisabsolutelyessentialtohave
experiencedguidance.IntheearlydaysofGcMAFavailability,untilproperaccreditationisestablished,findinga
doctorwhoisexperiencedintheuseofGcMAFandNagalasetestingwillbechallenging.
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The GcMAF Book
Chapter17
Differentiation and Cancer Grading

Cancercelldifferentiation.Aboutcancergrading.Macrophageseasilyfindundifferentiatedcells
becausetheydisplayahigherdegreeofcellsurfaceabnormality.GcMAFcureratesdependonthe
degreeofcancercellmembraneabnormality,whichcorrespondstothegradeofdifferentiationof
malignantcells.
Underamicroscopecancercellsdontlooklikenormalcells
Inordertounderstandhowcancersaregraded,weneedtohaveashorttalkaboutcancercelldifferentiation.
Supposeyouhavearipebanana.Asitgraduallygoesbad,firstafewminorbrownspotsappearonthesurface.
Then,asitapproachesinedibility,itlooksmoreandmorerottenanditbecomesprogressivelyeasiertomakethat
determination.Cancercellsarelikethat.Asthecancerprogresses,thecellslook(tomacrophages)increasingly
weirderandweirder,andasaconsequencetheybecomeprogressivelyeasiertoidentifyandkill.Hereshowit
works
Astheymature,normalcellsdifferentiateintospecializedcells.Cancercellsaredifferent:theydonot
differentiateintomaturespecializedcellstheyremainimmaturelooking.Cancerspecialiststhereforecallthem
undifferentiated.
Welldifferentiated(i.e.,earlystage)cancercellslookalmostnormal,butpoorlydifferentiated(moreimmature)
cellsdonotlookatalllikenormalcells.Theylooklikemoreandmorelikecancercells.Advancedcancersconsist
mostlyofpoorlydifferentiated(immaturelooking)orundifferentiatedcells.
Whenmacrophagesareactivated,theydevelopverylargenumbersofsurfacereceptorsthatareprogrammedto
spotcancercellsurfaceirregularitiesandthenlatchontothem.Sinceundifferentiatedcancercellshavemore
surfaceirregularities,theyareeasiertolocate.Whentheactivatedmacrophagesfindthemwell,bynowyou
knowthedrill.
Cancergrading
Theabovefactsareusefulwhentryingtounderstandcancergrading.Degreeofdifferentiationprovides
informationaboutcanceraggressivenessandprogressionbecausethemorenormal(i.e.,differentiated)acancer
celllooks,theloweritsgrade.Themoreabnormalorlesswelldeveloped(i.e.,undifferentiated)acancercell
appears,thehigheritsgrade.Thereareseveralgradingsystems,dependingontheinstitutiondoingthegrading
andthetumortype.Hereisadescriptionofatypicalthreetiergradingsystem:
Grade1:lowgradeorwelldifferentiatedcancercellsstilllookalotlikenormalcells.Thesecancersare
usuallyslowgrowing.
Grade2:intermediate/moderategradeormoderatelydifferentiatedcancercellsdonotlooklikenormalcells.
Theyaregrowingsomewhatfasterthannormalcells.
Grade3:highgradeorpoorlydifferentiatedcancercellsdonotlookatalllikenormalcells.Theyarefast
growingoraggressive.
Thereisneveranyabsolutecertaintyabouthowcancercellswillbehave,butgradeisausefulindicator.Alow
gradecancerwillgrowmoreslowlyandbelesslikelytospreadthanahighgradeone.Oncologiststakecancer
gradeintoconsiderationwhenponderingtreatmentdecisions.
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Poorlydifferentiated(immature)cancercellsarecoloredblue.
WhyisdifferentiationimportantintermsofGcMAF?
Themoreundifferentiatedacancercellis,theeasieritisforGcMAFactivatedmacrophagestofindit.
Likeanadvancedcancercell,Waldo(ofWheresWaldo?)standsoutinahugecrowdbecausehelooksdifferent.If
Waldowereacancercell,activatedmacroswouldfindhimeasilyandhedbetoastinminuteshislittleredand
whitestripedshirtandfunnyglasseswouldbeallthatwouldbeleftbehind.
Theweirderthecancercelllooks,theeasierthemacroscanrecognizeanddestroythem.Undifferentiated(i.e.,
advancedoraggressivecancercells)havemoreabnormalitiesontheircellsurfaces,sotoactivatedmacrophages
theylookmoreforeignthanearlierstage,welldifferentiatedcancercellswithfewersurfaceabnormalities.
Therefore,undifferentiatedcancercellsaremorerapidlykilledbyactivatedmacrophagesbecausetheystandout
(likeWaldo)inthecrowd.
WhenmacrosareactivatedbyGcMAF,theirgenomedramaticallyupregulatesexpressionofthereceptorproteins
thatidentifycancercells.MorereceptorstranslatesintomoredetectiveslookingforWaldo,soheislotseasierto
find.
Dr.YamamotoshowedthatcancercellsurfaceabnormalitiesmanifestedahighcorrelationwithGcMAF
effectiveness.Healsoshowedthatmacrophageactivationcauseddramaticincreasesinnumbersofmacrophage
cellsurfacereceptorsthatrecognizeawidevarietyofcancercellsurfaceabnormalities.
inhis2008paperonbreastcancerDr.Yamamotodescribesthesephenomenaasfollows:Thus,the
macrophagesactivatedbyGcMAFdevelopedenormousvariationofreceptorthatrecognizeavarietyofmicrobial
agents(e.g.,bacteriaandviruses)andabnormalitiesinmalignantcellsurfaces.Thisfundamentalnatureof
macrophagestorecognizecellsurfaceabnormality(nonselfingnature)isuniversaltoalltypesofcancers.Infact
weeklyadministrationof100ngGcMAFtocancerpatientsshowedcurativeeffectsonavarietyofcancers.Types
ofcancersofartestedareprostate,breast,colon,stomach,liver,lung(includingmesothelioma),kidney,bladder,
uterus,ovarian,head/neck,braincancers,melanomaandfibrosarcoma.EfficacyofGcMAFtherapyforcancers
dependsonthedegreeofcellmembraneabnormality.PrecisionofmeasurementofNagalaseactivityallowedusto
determinethedegreeofcellsurfaceabnormalitybythecurativerateduringGcMAFtherapy.Undifferentiated
tumorcellsarekilledmoreefficientlythandifferentiatedcells.Infactadenocarcinomasuchasbreastandprostate
cancercellsareundifferentiatedandkilledrapidlybytheactivatedmacrophageswhereaswelldifferentiated
cancercellssuchassquamouscarcinomacellsareslowlykilledbytheactivatedmacrophages.Thiscurativerate
appearstodependonboththeamountofreceptorsfortheparticularantigenonmacrophagesandtheamountof
antigensoneachcell.(Int.J.Cancer:122,461467(2008).Immunotherapyofmetastaticbreastcancer
patientswithvitaminDbindingproteinderivedmacrophageactivatingfactor(GcMAF).NobutoYamamoto,
HirofumiSuyama,NobuyukiYamamotoNaofumiUshijima)
Administrationof100ngGcMAFperhumanresultsinthemaximallevelofmacrophageactivationwhichdevelop
anenormousvariationofreceptorsthatrecognizeabnormalityinmalignantcellsurfaceandkillcancerouscells.
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Allmalignantcellshaveabnormalitiesintheircellsurface.Aseriesofglycolipid,glycoproteinandmucinantigens
havebeenidentifiedanddesignatedastumorassociatedantigen(TAA)onthecellsurfaceofawidevarietyof
tumorcells.WhenhumanmacrophagesweretreatedinvitrowithGcMAF(100pg/ml)for3hrandabreast
cancercelllineMCF7wasaddedwitheffector/targetratioof1.5,60%and86%ofMCF7cellswerekilledin4hr
and18hrincubation,respectively.(Int.J.Cancer:122,461467(2008).Immunotherapyofmetastaticbreast
cancerpatientswithvitaminDbindingproteinderivedmacrophageactivatingfactor(GcMAF).NobutoYamamoto,
HirofumiSuyama,NobuyukiYamamotoNaofumiUshijima)
Againinhis2008prostatecancerpaperDr.Yamamotocommentsontheconnectionbetweencellsurface
abnormalities,degreeofdifferentiation,andeffectivenessofGcMAF:EfficacyofGcMAFtherapyandcurativerates
ofvariouscancersbyGcMAFtherapydependonthedegreeof(cancer)cellmembraneabnormality,which
correspondstothegradeofdifferentiationofthemalignantcells.(TranslationalOncology.2008July1(2):65
72.ImmunotherapyforProstateCancerwithGcProteinDerivedMacrophageActivatingFactor,GcMAF.Nobuto
Yamamoto,HirofumiSuyama,andNobuyukiYamamoto)
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The GcMAF Book
Chapter18
The Cancer continuum and the 'Point of no return'

Stagesinthelifeofacancer.HowDr.Yamamotochosehispatientshadalottodowithgetting
100%curerates.TheoptimumpointinthecancercontinuumforGcMAFtherapy.Thebiggera
cancergetsthelesslikelyGcMAFwillwork.Trackingeffectivenessofallcancertherapieswith
NagalaseandAMAS.Anexpandingtumorsoonerorlaterreachesapointofnoreturn.
TheCancerContinuum
Onecouldviewthelifeofanycancerasacontinuumstartingwithonecell(onthefarleftside)andcontinuing
throughgrowthandmetastasisandfinally(tothefarrightside)asamassivecomplexofmetastatictumors
comprisedofmanybillionsofcells,occupyingmultiplelocationsinthebodyacancerthathasbecomelarge
enoughtooverwhelmandkillitshost.Cancerspecialistsstagecanceraccordingtowhereitliesonthis
continuum.
Howcancergrowsandspreads
Malignanttumorsbeginwithonecancercell.Thiscellmultipliesbydividingtobecomeanexpandingtumormass.
Atfirst,liketermitesinyourhouse,cancerspersistentlygnawaway,expandingandencroaching.Theycapture
territorybyspreadinglocallyandpushingasidehealthytissues.Eventuallytheybreakthroughthebarriersthat
keepthemlocalandspilloverintothelocallymphchannels(whichtrytocontainthem).Again,ifunchecked,the
cancercellsnextmanagetocrashthebarrierstothebloodstream,which,likeenemysubmarines,theysilently
navigatetofarflungregionsofthebodywheretheyspawnnewcolonies.Uptothispointthebodyscancer
containmentbarriershaveworked.Bybreakingout,thecancerhasbecometheinsidious,mostfeared,most
deadlyform:metastaticcancer.
Priortometastasis,almostallcancersareconsideredcurable.Aftermetastasis,theoddsofacureplummet.But
evennow,atthis,themetastaticstage,oncologistscontinuelobbingchemotherapeuticbombsandradiologists
stillblastawaywithparticlebeams.Inthecurrentsystem,thesewellintendedeffortsareforthemostpartfutile:
metastaticcancermaybeslowedbutisrarelycured.Butasyouwillsee,thestandardtherapiesthatreduce
tumorburdengreatlyimprovetheoddsthatGcMAFwillbeeffective.
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Optimumtreatmentdependsonthestageofthecancer
Optimumtreatmentstrategiesvarydependingonthetypeandstageofthecancer.Intheearlieststage,priorto
detectiononimaging(butpositiveonAMASorNagalase),alternativetreatmentapproachesworkbest.Thisistrue
becausenaturaltherapiesenhancethebodysownanticancersystemsratherthandamagingthem.Bythetime
acancerhasgrowntothesizewhereitcanbeseenonimaging,however,thetoxictriad(surgery,radiation,
and/orchemotherapy)havebecomenecessary.
EarlydetectionistheHolyGrail
Theeffectivenessofanytherapy,includingGcMAF,haseverythingtodowiththepointalongthiscontinuumat
whichtreatmentisinitiated.Earlydetectionhasjustifiablybecomethegospelofcancertherapy.Thesoonera
cancerisdetected,regardlessoftype,thebettertheprognosis.Catchingacancerbeforeitmetastasizesis
especiallyimportant.Wewanttomovethepointofdetectionasfartotheleftaswecan.AMASandNagalase
testingwilldothat.
Metastaticcancer
Inmetastaticdiseasethetumorhasspreadbeyondthereachofsurgery,andchemoandradiationhavenot
succeededatcontainment.PandorasBoxisnowwideopen.ThesearethepatientsonwhomDr.Yamamoto
chosetofocus:theoneswithdemonstrableearlymetastaticdisease(definedbythepresenceofNagalase)butfor
whomthestandardtherapieshadfailed.Weeklyintramuscularinjectionsof100nanograms(100billionthsofa
gramyoudneedapowerfulmicroscopetobeabletoseeit!)for36monthscuredeverysingleoneofthese
incurablepatients.
Yamamotosworkpushedtheenvelopeinthathecuredcarefullychosenearlystagemetastaticcancers.Astumor
sizeandmetastasesincrease,thechanceofsuccesswithGcMAFdeclines.Withlarger(greaterthan2cm.)
metastatictumors,GcMAFmaynotworkoralongerdurationoftreatmentmayberequired.Theprobabilityof
successwithGcMAFcanbeimprovedbyreducingtumorburdenviachemo,radiation,andsurgery.Sooneror
later,however,asacancercontinuestogrow,evenlargenumbersofhighlyactivatedmacrophageswontbe
enoughtocarrytheday.
ChoiceofcancerstagehadeverythingtodowithYamamotossuccess
WhydidDr.Yamamotochoosetotreatpatientsatthepointinthecancercontinuumwhereconventional
therapieshadfailed?Obviously,ifconventionaltherapieshadcuredthesecancers,therewouldbenofurtherneed
forGcMAForanyothertreatment.YamamotochosethisgroupofpatientsbecauseheknewthatGcMAFwouldbe
mosteffectiveinpatientswithlowtumorburdenwhohadflunkedthemainstreamapproachandstillhadcancer
growingsomewherewithinthem.
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ElevatedNagalaselevelstoldhimthatmetastaticdiseasecontinuedtolurksomewhereinthesepatientsbodies.
AllcancercellsmakeNagalase(andnormalcellsnevermakeit),sothepresenceofNagalaseissynonymouswith
thepresenceofcancer.
YamamotoalsoknewthatlargertumorswouldbeharderforGcMAFactivatedmacrophagestovanquish,sohe
chosepatientsinwhomthebulkofthecancershadbeenremovedandinwhomtherewasgreatreasonto
believethatthetumorburdenthoughmetastaticwasatitslowestpossiblepoint.
Yamamotosresearchpatientshadspecialadvantages.First,theywereintheearlieststageofmetastaticdisease.
Secondbecausetheyhadenduredtheappropriateconventionalcombinationoftherapiestheirtotaltumor
burden(thenumberofcancercellsremainingaftersurgery,radiation,and/orchemotherapy)wasverylow.Asa
consequence,GcMAFactivatedmacrophagesweregivenahugeadvantage.Theratioofactivatedmacrophagesto
cancercellswashigh,andsothemacroshadnotroublepolishingofftheircancermeal.
Yamamotosstrokeofbrilliancewasthis:hechosepatientsatthepointonthecancercontinuumwhereGcMAF
wouldexertthegreatestimpact.Thispointearlymetastaticdiseasewithrecentlyminimizedtumorburden
thusprovidedGcMAFwithatremendouslikelihoodofacure.Andthatsexactlywhathappenedineverysingle
case.
Ifhehadtreatedearlierbeforestandardtherapieshadhadtheirchancetosucceedorfailhewouldnothave
knownforsurewhetherornotthesepatientsevenhadmetastaticdisease.Ifhehadwaitedlongertotreat,he
wouldgiventheshrunkentumorstimetogrowback(rebulk),andthuswouldhaveriskedlosingsomeofthe
patients.
Thepointofnoreturn
Individualcancersaredifficulttocategorizeandimpossibletoquantify,sowearedealingwithprobabilitieshere.
Atsomepoint,however,thecancerand/oritsmetastaseswillgainenoughmasstobeabletothwartthebest
effortsofdebulkingfollowedbyGcMAF.ThisiswherethecancershrugsofftheGcMAFeffectbecauseithasnow
growntothatsizeatwhichitisnowaddingnewcellsfasterthantheactivatedmacrophagescandevourthem.
Themealisjusttoobig.Icallthisthepointofnoreturn.Itsacancercliff,sotospeak,beyondwhichno
amountofGcMAFcouldrescuethepatient.
Insomecasessurgicalremoval,radiationand/orchemotherapymightdebulkthetumorbackdowntoasize
wheretheGcMAFcouldbeeffective.Thesekindsofdecisionswouldobviouslyhavetobemadebyanoncologist
experiencedinGcMAFtherapy.
IsGcMAFeffectiveinearlycancerstoosmalltoshowuponimaging?
Successratesshouldapproach100%inthesecases.(AgoodreasontoimplementNagalasescreeningforthe
massesassoonaspossible.Fornow,gogetanAMAScancerscreeningtest.)
IsGcMAFeffectiveinearlysmallcancerslargeenoughtoshowuponimaging?
WealsodontknowhoweffectiveGcMAFwouldbeinearlydisease,butthereisexcellentreasontobelievebased
onDr.Yamamotosstudiesthatall,oralmostall,earlysmalltumorsthathavenotyetmetastasizedwould
respondtostandardtherapiesfollowedbyGcMAF.BasedontheoutcomesreportedinDr.Yamamotosstudies,
effectivenesswouldapproach100%forcancersthathavebeenrecentlydebulkedandareintheearlystagesof
metastasiswhenGcMAFtherapyhasbeenbegun.
IsGcMAFeffectiveinadvanced(largemetastatic)cancers?
Dr.NobutoYamamotoshumanstudiesfocusedonlyontheearlygroup.Wehavenoresearchderiveddata
addressingtheissueofGcMAFeffectivenessinmoreadvancedcancers.Thestudieshavenotyetdone,andwe
dontknowhowadvancedacancermightbeandstillrespondtoGcMAF.Theremaywellbegreatvariabilityfrom
onepatienttoanother.
TrackingeffectivenessofallcancertherapieswithNagalaseandAMAS
Ifcancerispresent,Nagalaseand/orAMASwillbepositive.
NagalaseandAMASarenotonlyqualitativemarkers,however,theyarealsoquantitativebywhichImeanrising
orfallinglevelstelluswhatthecancerisupto.Nagalase/AMAScanthereforebeusedtomonitorthe
effectivenessofanycancertreatment,includingbothdrugandalternativetherapies,separatelyorin
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combination.
Nagalase,AMASandthepointofnoreturn
Normally,withGcMAFtreatment,serialNagalase(orAMAS)levelswilldecreaseovertime.Thistellsusthe
treatmentisworking(i.e.,adecliningcancercellpopulationismakinglessNagalase).If,however,themalignant
tumormasshadachievedthepointofnoreturnbeforeGcMAFtherapyhadbeeninitiated,theNagalaseand
AMASlevelsmightdropinitiallybuteventuallywouldcontinuerising,indicatingtheGcMAFactivatedmacrophages
havenotovercomethecancer,andthatitcontinuestogrow.
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The GcMAF Book
Chapter19
GcMAF Therapy Guidelines

Dosage.Frequencyofadministration.Routeofadministration.Durationoftreatment.Sideeffects
andtoxicity.Contraindications.PossibleGcMAFinhibitors.
GcMAFDosage
Inhispublishedhumanstudies,Dr.Yamamotoalwaysusedthesamedose:100nanograms(ng)perweekina
singleinjection.
100nanograms(100billionthsofagram)isanextremelysmallamount.Youwouldntbeabletoseeitwithouta
microscope.
Frequencyofadministration
Dr.Yamamotodeterminedthatthehalflifeoftheactivationeffectinanactivatedmacrophageisapproximately6
days.Therefore,hechoseaoneweekintervalbetweenGcMAFdoses.
Moreisnotbetter!
Itisimportantnottoerroneouslyassumethatif100ng/weekisgood,200ng/weekmustbebetter.Ahigher
dosewouldalmostcertainlybecounterproductive,akintoshootingoneselfinthefoot.Itislikewiseimportantnot
toassumethatifonceaweekisgood,twiceaweekwouldbebetter.Thereisnoclinicalorresearchdata
suggestingthatmorefrequentormorepotentdosingwouldimproveoutcomes.Higherdosesand/ormore
frequentadministrationswouldprobablyreducetheeffectivenessGcMAFmacrophageactivation.AccordingtoDr.
Yamamoto:
Sincethehalflifeoftheactivatedmacrophagesisapproximately6days[23,24],weeklyadministrationsof100
ngGcMAFwerefoundtobethemosteffective(YamamotoN,SuyamaH,NakazatoH,YamamotoNY,KogaY.
2008c.ImmunotherapyofmetastaticcolorectalcancerwithvitaminDbindingproteinderivedmacrophage
activatingfactor,GcMAF.CancerImmunolImmunother57:10071016.)
Routeofadministration
Allpatientsweregivenintramuscular(IM)injectionsofpureGcMAF.
IMinjectionsarenecessarybecauseoraladministrationwouldexposetheGcMAFproteinmoleculetogastric
hydrochloricacidandpancreaticproteaseenzymes,resultingindegradationanddeactivation.
Durationoftreatment
InDr.Yamamotosstudiesthedurationoftreatmentvariedfrompatienttopatientanddisordertodisorder:
100%ofnonanemicHIVpatientswerecuredinlessthan18weeks.
100%ofnonanemicearlymetastaticbreastcancerpatientswerecuredin1622weeks.
100%ofnonanemicearlymetastaticcolorectalcancerpatientswerecuredin3250weeks.
100%ofnonanemicearlymetastaticprostatecancerpatentswerecuredin1425weeks.
Sideeffectsandtoxicity
Therehasneverbeenanadversereaction,sideeffect,ortoxicreactiontopureGcMAF.Becauseitsmolecular
structureisidenticaltoGcMAFmadebythebody(i.e.,bioidentical),thereisabsolutelynoreasontoexpectthat
pureGcMAFwouldcauseanykindofproblem.OurgeneticprogramcontainsthecodeforbuildingGcMAF
molecules.Innormal,healthybodies,thegenesforGcMAFproductionareexpressed,andongoingGcMAF
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productionmakessureourmacrophagesarecontinuouslyactivatedsothattheycaneffectivelyaddressthe
ongoingonslaughtofbacteria,viruses,parasites,andnewlyformingcancercells.Whenvirusesandcancercells
obstructGcMAFproductionbyreleasingNagalase,thisfinelytunedmechanismsbreaksdown,GcMAFdwindles,
andmacrophagesslowdownandstop.Nowthewelcomematisout,thedoorisopen,andtheintruderscome
marchingrightin.ReplacingthedeficientGcMAFwithexactlythesamemoleculethatismissingguaranteesthere
willbenoadversereactions.
Theadministrationofimpure(nonbioidenticalorcontaminated)GcMAFcarriesahighpotentialforsideeffects
andadversereactions.Italsomightnotwork.InChapter21Iaddressthechallengingproblemofbootlegs,
counterfeits,knockoffs,wannabesandcertificationtoensurepurity.TheimportanceofaestablishingaGcMAF
certifyingauthoritycannotbeoverstatedamarketfloodedwithineffectiveproductswouldprovideauthorities
withagoodreasontoshutdownallproduction.
DiseasesforwhichDr.YamamotohasindicatedhefeelsGcMAFwouldbeeffective:
Tuberculosis
Cancers(all)includingprostate,breast,colon,stomach,liver,lung(includingmesothelioma),kidney,
bladder,uterus,ovarian,head/neck,braincancers,melanoma,andfibrosarcoma
InfluenzaAandB
Herpesviruses
HepatitisBandC
HumanImmunodeficiencyVirus(HIV)Allotherretroviralinfections
EpsteinBarrVirus
PotentialObstaclestoGcMAFeffectiveness
ProteaseinhibitorsGcMAFmightnotworkforHIVpatientstakingproteaseinhibitors.Inmacrophage
phagolysosomes,proteasesworktodigestcancercellsandviruses.Proteaseinhibitionmightinterferewith
phagolysosomalproteaseactivity,slowingdigestionofphagocytizedinvaders,thusindirectlyobstructingthe
macrophageactivatingeffectoftheGcMAF.
OpiatesAccordingtoDr.Yamamoto,opiates(morphine,Demerol,opium,oxycodone,hydrocodone,Percodan,
Percocet,Vicodin,Norco,etc.)mayblockGcMAFsmacrophageactivatingeffect.
AnemiaAlackofsufficientredbloodcellsmaycompromiseGcMAFeffectiveness.Anemicpatientswereexcluded
fromallofDr.Yamamotosstudies.
InsufficientmacrophagesIfapersondoesnthaveenoughmacrophagesormonocytes(theprecursorcellsthat
becomemacrophages),theymightnotrespondasbrisklytoGcMAFasapersonwithnormal
macrophage/monocytelevels.Patientswithlowwhitecellcounts(orcompromisedimmunefunctioning)should
takehydrolyzedwheyproteinwhichwillencouragethebonemarrowtomakemorewhitebloodcells.Makesure
youpurchasecoldprocessedhydrolyzedwheyproteinmarketedbyacompanythatspecializesinsupplying
nutritionalmedicinestophysicians(PureEncapsulationswouldbeanexcellentchoice).Thewheyproteinfoundin
healthfoodstoresisprocesseddifferentlyandwouldnotbeeffective.
Medicalmonitoringrequired
Goodintentionscoupledwithpoortrainingcanleadtodisaster.AnyonewhoallowsanovicetoadministerGcMAF
ismakingabigmistake.Onceavailable,GcMAFadministrationmustalwaysbemonitoredbyaphysiciantrainedin
itsuse.
Ratherthanasubstituteforconventionalcancertherapy,GcMAFisanadjuncttoit.GcMAFeffectivenessdepends
onoptimumadministrationofconventionalmainstreamcancertherapies.SubstitutingGcMAFforconventional
therapiesforanimageablecancercouldresultinapreventabledeath.
MedicallyunsupervisedadministrationofGcMAFwouldbeagraveerrorforthefollowingreasons:
GcMAFeffectivenessdependsonoptimumadministrationofconventionalmainstreamcancertherapies.
Tumordebulkingusingsurgery,chemotherapy,and/orradiationiscrucialbecauseGcMAFworksbeston
smallercancers.Debulkingmovesthepatientbackfromthepointofnoreturn.
Physiciansarenecessarytodiagnoseandtreatconcomitantmedicaldisorders.
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YourdoctorwillneedtomonitorprogressbycheckingAMASorNagalaselevels(whenavailable)inorderto
determinewhetherornottheGcMAFisworking,andtoknowwhenitstimetodiscontinueit.
Cancerimaging,orderedandinterpretedbyphysicians,providescrucialinformationaboutcancerprogress.
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The GcMAF Book
Chapter20
Why Not Skip Conventional Cancer Therapies and Just

Take GcMAF?
Whynotjustskipthesurgery,radiation,andchemoanduseGcMAFallbyitself?
Somecancerpatientsinthehopesofsidesteppingtheslash,burn,andtoxicdiscomfortsofsurgery,radiation,
andchemotherapymightconsideroptingforGcMAFtherapyallbyitself.IfGcMAFworks,theyask,whybother
withthenastystuff?
Notsofast.Formostcancerpatientsthiscertainlywouldbeaverybadidea.Hereswhy:GcMAFworksbetteron
smallertumors.Despitethemanydrawbacksofthebigthree,theyallreducetumormass.Thebasicideahere
isthatactivatedmacrophagesgobbleupcancercells,butaswithyourlunchthelessyouhavetoeat,the
quickerandeasieryoucanfinishthemeal.Andjustasthereisanupperlimittothesizeofamealyoucould
consume,thereislikewisealimittotheamountoftumormaterialeventhemostaggressivelyactivated
macrophagescanpolishoff.
Thinkaboutit:thebiggerthemess,themorespongesyoudneedtocleanitup.Samestrueforcancer.Surgery,
radiation,andchemoalldiminishthesizeofthetumorand/oritsmetastases(thisiscalleddebulking).Smaller
tumorsareeasiertargetsfortheGcMAFactivatedmacrophagesbecausewithfewercancercellstodevour,their
workloadislightened.So,youcancerpatientsdreamingofaquick,simple,easycure,listenup:avoidingthe
recommendedconventionaltreatmentsforyourcancerwouldnotbeawisemove.UsingGcMAFonadebulked,
smallertumormaymakethedifferencebetweencureandnocure.
Anotherfaultylineofreasoningmightgosomethinglikethis:IcouldtrytheGcMAFalone,andifitdoesntwork,
thenIcanstillgobackanddothesurgery,chemo,and/orradiation.Thisapproachmightprovefoolhardy
becausewaitingcouldallowcancergrowthbeyondthepointatwhichitisstillreversible.Again,itsbesttogowith
thedebulkfirststrategy.
Dr.Yamamotodebulkedfirst
ItwasnoaccidentthatDr.Yamamotochosetolimithisgroundbreakinghumanresearchtocancerpatientswho
hadrecentlyreceivedoptimumdebulkingprocedures.Eachofthebreast,prostate,andcolorectalcancer
patientstowhomheadministeredGcMAFhadrecentlycompletedtheappropriatemainstreamcourseof
treatmentfortheircancer.Surgery,radiation,and/orchemo(inwhichevercombinationwasindicatedinthat
particularpatientscancer)hadbeendone.BecausethesepatientsstillhadelevatedNagalaselevels,heknew
theyhadmetastaticdisease.Theconventionaltherapieshadnotcuredthem.Thepatientshad,however,been
debulked,andthismadeahugedifferenceintheabilityoftheGcMAFtodoitsjob.Yamamotothusprovedthat
combiningmainstreamdebulkingtherapieswithGcMAFcouldproduceacurein100%ofearlymetastaticcancer
patientsinwhomtheconventionaltherapiesalonehadfailed.
HadYamamotochosenidenticalpatients(intermsofcancertypeandstage)andadministeredtheGcMAFpriorto
theconventionaltherapies,healmostcertainlywouldhavehadsomefailures.Hadhewaitedmonthsoryearsfor
theseuncuredcancerpatientstumorstoreturn(torebulk,asitwere),again,hiscureratewouldcertainly
havebeenlower.Debulkingworks,isanimportantfeatureinthecurativeprocess,andshouldnotbeavoidedor
delayedinthehopesthatGcMAFwillaccomplishthejoballbyitself.Onewhoproceedsdownthisavenuerisks
losingthechanceatacurethatearlydebulkingtherapiesfollowedbyGcMAFmighthaveprovided.
ReducingviralloadinHIV
TheabovelogicalsoappliestoHIV.UsingappropriatedrugcocktailstoreduceHIVviralloadwillgiveGcMAFabig
headstartintermsofdevouringtheremainingviruses,andthiscouldmakethedifferencebetweencureandno
cure,betweenlifeordeath.(BecauseproteaseinhibitionmightinterferewithGcMAFeffectiveness,itwillprobably
provenecessarytodiscontinueproteaseinhibitorswhileonGcMAFtherapy.HIVpatientsonceGcMAFisavailable
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willneedtodiscusstheseconcernswiththeirphysician.)
Theexceptiontothedebulkingrequirement:earlycancerstoosmalltoimage
Intheearlieststagesofcancer,whenthemassisstillverysmall(<5mm),thedebulkfirstrequirementdoes
nothold.IfapatientknowsfromanelevatedNagalaselevelthatheorshehasacancergrowingsomewhere,but
itisnotbigenoughtobeseenonimagingandtherearenolocalizingtestslikeapositivePSA(prostate),BrCA1or
2(breast),orCA125(ovarian)toidentifyitswhereabouts,thenusingGcMAFalonewouldbeadvisable.If,over
time,theNagalase(orAMAS)levelsdropbacktonormal,onecanassumethecancerwasnippedinthebud,
curedbeforeitgotbigenoughtobeseenonimaging.IfIhadatumorthatwasbigenoughtoseeonimaging,
however,Idwantasurgeontotakeitout.IdstilluseGcMAF(ifitwereavailable),justincasethesurgeondidnt
getitall.
SinceNagalasetestingisnotyetavailable,usingittofindcancerearlyremainsapurelytheoreticalnotion.
Meanwhile,AMAStestingcanbeusedtodetectearlycancersandtotrackcancertherapy.
HowcanItreatitIcantseeit?
Somephysiciansfinditdisturbingtobetreatinganinvisiblediseasethathasnosymptoms,nophysicalsigns,
andeveneludesstateoftheartimaging.Theseoldschoolersremainhesitanttotreatiftheycannotmakea
formaldiagnosisusingthetoolstheyunderstandbest.Fearnot,thisisanoutmoded,vanishingbreedthatinthe
ageofmolecularandgeneticmedicineisheadedforextinction.
Biochemicaltestingprovidespowerfulassistanceindiagnosingtheearliestsignsofmanydiseasestates.Mybook
OutsmartingTheNumberOneKiller(http://www.renewalresearch.com/tnok/TNOKtext.pdfandslideshow
summaryathttp://www.renewalresearch.com/tnok/TNOK.pdf)explainshowtousemolecularmarkerstoidentify
andreverseriskofheartattackandstroke.Now,withNagalasetesting,wehaveatoolforveryearlydetectionof
cancer,thenumbertwokiller.
Usingstateoftheartbiochemicalmarkers,themolecularmedicineearlywarningsystemenablesearlier
diagoses.Detectedinitsinfancy,adevelopingdiseasecanusuallybereversedusingnontoxicnutritional
medicines(diet,phytonutrients,herbs.vitamins,minerals,aminoacids,essentialfattyacids,hormones,enzymes,
andhomeopathicmedicines).Oncethediseasehasprogressedtoaheartattackorlargetumor,however,reversal
becomesfarmoredifficult,naturalmedicinesarelessoftenanoption,andtheneedtoresorttostrongdrugs
loomslarge.
Soheresmyunsolicitedadvicetothosedoctorswhowouldresistidentifyingandtreatingtheearliest
manifestationsofdisease:studymolecularbiologyandnaturalhealingmethods.Goodthingswillhappen!
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The GcMAF Book
Chapter21
Knockoffs, Wannabes, Bootlegs, Counterfeitsand

Certification
HypeandmisinformationaboutGcMAF.Ineffectiveandpotentiallytoxicproducts.Pure,realGcMAF
vs.phonies.TypesofGcMAFknockoffs.Aboutpurity.GcMAFproduction.Contamination.Certification.
Caveatemptor!!
Becareful!
TheemergenceofGcMAFtherapyandNagalasetestingasviablemethodsfortreatingandpreventingcancerwill
inevitablybeaccompaniedbygenuineconcernanddebateabouthowbesttoputthesevaluabletoolstowork.
Parallelingthesepositiveeffortstopreventsufferingandsavelives,negativeforceswillexudefromtheswampof
diabolicalhumangreed.SlickhucksterswillofferstuffcalledGcMAFthatdoesntworkandmayevenbe
harmful.Ineffectiveimitationsandcounterfeitproductswillappear,includingknockoffs,copycats,phonies,
bootlegs,andwannabes.Therewillbenaysayersanddisinformationcampaignstryingtoconvinceyouthisisalla
bighoax.And,asweallknow,theinternetisrifewithhypeandmisinformationGcMAF/Nagalasewillbeno
exception.
WhatsapersondesperatelyinneedofcancerorHIVtreatmenttodo?Theurgetobuyintofalsehopemaybe
difficultorimpossibletoovercome.
Pure,realGcMAFvs.thephonies
Becauseitisidenticaltothatwhichthebodymakes(i.e.,bioidentical),pureGcMAFwillnevercausesymptomsof
anykind.WhensymptomsappearinanindividualtakingGcMAF,thecauseisimpurity,contamination,orboth.
Thephonieswillcomeinpackagesthatlookexactlylikequalitycertifiedproducts.Packagingreplication
technologyhasadvancedtothepointwhereevenbrandnamemanufacturerscanttellthedifferencebetween
theirownproductsandtheknockoffswithoutchemicallytestingtheingredients.Peoplewilldieneedlessly
becausetheyputtheirfaithinphonyGcMAFproducts.
TypesofGcMAFknockoffs
AhugemarketforGcMAFwillemergeasitbecomesahouseholdword.Thealreadysubstantialriskofimpure,
contaminated,andinactiveproductswillthenskyrocket.Hucksterswilloozefromthewoodwork,hawking
GcMAFthatwillrangefrominactiveandharmlesstotoxicanddangerous.Therewillbedifferenttypesof
knockoffs:
Bootlegorwannabeversions:seriousattemptstomakeGcMAFthathavefallenshortandareimpure,
contaminated,and/orpotentiallytoxic.Bootlegversionscanbepurebutdirty,containingcontaminantsthat
causemuscleaches,flulikesymptomsorfatigue.Abootlegcouldalsobepure(inthesenseofclean),butnon
bioidentical,andthereforetooweakinitsmacrophageactivatingpowertobeeffective.
Phonyversions:packagedtolookexactlyliketherealthing,buttotallyinactive.TheGcMAFmightbepowdered
sugar,starch,orany(hopefully)harmlesswhitepowderthat,likeGcMAF,disappearswhenputintosolutionwith
water.Thephonyproductmightsimplycontainnothingatall:severalhundrednanogramsofGcMAFinavial,
sanswater,wouldbeaboutthesizeofasmallspeckofdust.
Asmentionedabove,nowadaysitispossibletosoexactlycopyabottlingandpackagingprocessthateventhe
authenticmanufacturingcompanycanttell,byexaminingthepackagingalone,whetherornottheproductinside
isreal.Chromatographytestingisrequiredtomakethatdetermination.
IllneverforgetanexperienceIhadseveralyearsago.Apatienttoldmeshehadbeeninjectinghumangrowth
hormone(hGH)thatshehadbeenobtainingfromasourceininTijuana,viaSanDiego.Sheshowedmethe
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packageanditlookedrealenough,asifithadcomedirectlyfromthemanufacturer:perfectbox,redplastic
cappedvialinside,flawlesslabel,nothingsuspicious.IsuggestedwetestherbloodforhGH,andwhenwedid,
therewasnonethere!Shehadbeeninjectingveryexpensivewater!Obviouslythiswasaperfectknockoff,and
whenIcontactedthemanufacturer(oftherealproductamajorbrandnamehouseholdworddrugcompany),
theytoldmethateventheywereunabletotellbythepackagingalonewhetherornotagivenproductwasthe
realthing.Theyhadtotesttheingredientstobesureitwastheirs!Perfectpackagingtellsyounothing.
PurityandGcMAFproduction
Apureproteincontainsonlymoleculesofasingleprotein,andnoothermoleculesofanykind.Impuritiescause
compromisedeffectiveness,adversereactions,andsymptomsoftoxicity.MakingpureGcMAFisnotasimple
undertaking.Sofar,theonlyhumanbeingtohavesucceededinmakingpureGcMAFisProfessorNobuto
Yamamoto.Theprocessisnotthatcomplicated,however.Dr.Yamamotoprovidesspecificinstructionsinhis
researchpapers.AgoodbiochemistwithproperequipmentcouldeasilymakeGcMAF.Therawmaterialsare
inexpensiveandalargescalemanufacturingoperationshouldmakeforapricetagthatswithinreachofthe
averageperson.
GcMAFcanbemadeviarecombinantDNAtechnologybyinsertingasmallsectionofDNAintothebacteriumE.
coli.ThispieceofDNAreprogramsthebacteriumtomakeGcMAF.Animpuritycanbeintroducedatanystepin
thismultistageproteinmanufacturingprocess.
Forexample,oncethesereprogrammedbacteriaareincubatedandhavegeneratedtheGcMAFfromtheirrevised
geneticprogram,thenewlymadeGcMAFmustbeseparatedfromtheE.coliandthenpurified.IfsomeoftheE.
coli(oranyofthemanyotherchemicalsgeneratedbyE.coli)areleftbehindwiththeGcMAF,youhavea
troublesomeimpurity.EndotoxinsareanespeciallycommonE.colibyproductimpuritythesetypicallycauseflu
likesymptoms.Solventsandsurfactantscanlikewisebeleftbehindinthepurificationprocess.
ImpuritiesdontnecessarilyaffectthemacrophageactivatingpoweroftheGcMAFproduct,buttheycananddo
causesideeffectssuchasfatigue,weakness,malaise,andmuscleandjointpain.Thesesymptomscanbeso
uncomfortableforthepatientthatitbecomesimpossibletotoleratethebeneficialeffectstheywouldotherwise
obtainfromtheGcMAF.
Contamination
ContaminationoccurswhenaforeignagentgetsintotheGcMAFproductfromtheoutside(ratherthanbeing
introducedasapartofthemanufacturingprocess).Contaminantscancausesymptomssimilartothosedueto
impurities(fatigue,weakness,malaise,andmuscleandjointpain).Contaminantscanbeairborne,orcarriedinto
the(otherwisesterile)productionlabenvironmentbyhaplesshumansontheirhands,feet,orclothing.They
canenterviathelabswaterandairsupply.Acoughfromapersonwithabacterialrespiratoryinfectioncould
causebigproblems.Contaminationviamolds,viruses,andbacterialspeciesisverycommoninbiotechproduction
facilitiesandrequireshygieneprecautionssoelaboratetheymakesurgicalscrubbinglooklikeacakewalk.
Enteringabiotechproductionfacilityislikedressingforawalkinouterspace:cap,gloves,fullbodygown,mask,
booties.Youdontneedoxygentanks,buttheairyoubreathemustbefilteredtoremovedust,toxicchemicals,
andpathogenicmicroorganisms.
Potency(quantifiedasmacrophageactivation)
AssumingwehaveapureGcMAFproduct,thenwhataboutactivityorpotency?Intheworldofmacrophages,
potencyisactivity.ThedegreetowhichGcMAFactivatesmacrophagesisourmeasureofpotency.Onlypure,
bioidenticalGcMAFwillprovidethelevelofoptimummacrophageactivationnecessarytoreversecancersandviral
infections.
Extremelysensitivereceptorsonthesurfaceofmacrophagesarehappiestwhentheyarestimulatedbypure
GcMAF.Rearrangeevenafewatoms,muchlessafewaminoacids,andtheGcMAFwillnotlookthesameto
thosereceptorsandeithertheywontrecognizeitatall,ortheirresponsewillbehalfhearted.So,beyondpurity,
theGcMAFproductmustalsobetestedformacrophageactivatingpower.Thistestliterallyasksthemacrophages,
DoyourecognizethisparticularbatchofGcMAF?And,Howmuchdoesitactivateyou?Becausethisinvolves
workingwithlivehumanmacrophages,itisnoteasy.Todeterminethemacrophageactivatingpowerofpure
bioidenticalGcMAF,ProfessorYamamotoquantifiedrateofphagocytosisofcancercells(a30foldincrease),rateof
generationofsuperoxideradicals(15fold),andrateofmacrophageproliferation(40fold).
Certification
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Itwillbenecessarytoestablishaconsumerprotectioncertificationprogramwherebyproductofferingswhen
theydobecomeavailablewillbesubjectedtorigoroustestingandthencertifiedforbothpurityandactivity.
Caveatemptor!!*Buyerbeware!!
Inthemeantime,ifyouareofferedaGcMAFproduct,beawarethatatthispointintimethereisnoproduct
thathasbeenindependentlytestedandverifiedaspure,contaminantfree,toxinfree,bioactiveGcMAF.Weare
hopingthissituationwillchange,andwhenitdoes,mywebsite,www.gcmafnow.org,willreportit.
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Appendix1:AboutDr.NobutoYamamoto
The GcMAF Book
Appendix1
About Dr. Nobuto Yamamoto
Dr.NobutoYamamotowasborninJapanonApril25,1925.Thoughacceptedtothreemedicalschools,hechose
tofocusonbiochemistryingraduateschool.
AftergraduationheservedasassociateprofessoratGifuMedicalSchool,Japanuntil1959.
VisitingscientistintheMicrobiologyGroupattheInstituteforCancerResearch(FoxCancerCenter),Philadelphia,
PA,from1959to1961studiedthegeneticevolutionofbacterialviruses.
1964:joinedthefacultyatTempleUniversity,Philadelphia,PA,asHeadofVirologyandGeneticsoftheFels
CancerResearchInstitutewhereheserveduntil1980.
In1980Dr.YamamotowasappointedprofessorofMicrobiologyandImmunologyatHahnemannUniversity
SchoolofMedicine,wherehecontinuedtostudyviralevolutionandrevivedhisgraduatestudyofimmunology
from35yearsbefore.Hisimmunologicalstudiesemphasizedmechanismofmacrophageactivationanddiscovered
GcMAF.
WhenDr.YamamotoretiredfromHahnemannUniversityin1990,hewasaskedtoreturntoTempleUniversity
MedicalSchoolasaResearchProfessorofBiochemistry.Therehestudiedthetumoricidalcapacityofmacrophages
activatedbyGcMAFandcancertherapywithGcMAF.
In1994Dr.YamamotobecamethefounderanddirectoroftheSocratesInstituteforTherapeuticImmunology,
wherehecontinuestostudythetherapeuticefficacyofGcMAFforavarietyofcancersandHIV.
TomakeadonationsupportingDr.Yamamotoswork,youcouldsendacontributionto:SocratesInstitutefor
TherapeuticImmunology,104066thAvePhiladelphia,PA191263305.
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Appendix2:TheYamamotoPapers
The GcMAF Book
Appendix2
The Yamamoto Papers

Dr.Yamamotos2008breastcancerpaperentitled:Immunotherapyofmetastaticbreastcancerpatientswith
vitaminDbindingproteinderivedmacrophageactivatingfactor(GcMAF).(Thisisanabstract.Youcanaccessthe
fullpaperbyclickinginupperrighthandcorner.)
Dr.Yamamotos2008prostatecancerpaperentitled:ImmunotherapyforProstateCancerwithGcProtein
DerivedMacrophageActivatingFactor,GcMAF.
Dr.Yamamotos2008colorectalcancerpaperentitled:Immunotherapyofmetastaticcolorectalcancerwith
vitaminDbindingproteinderivedmacrophageactivatingfactor,GcMAF.(Thisisanabstract.Youcanaccessthe
fullpaperbyclickinginupperrighthandcorner.)
Dr.Yamamotos2008HIVpaperentitled:ImmunotherapyofHIVInfectedPatientsWithGcProteinDerived
MacrophageActivatingFactor(GcMAF).
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Appendix3:AbouttheAuthor
The GcMAF Book
Appendix3
About the Author
TimothyJ.Smith,M.D.hasbeenstudyingandpracticingalternative,nutritional,andconventionalhealing
principlesforover40years.A1970graduateoftheUniversityofCincinnatiCollegeofMedicine,hecompletedhis
internshipatthePacificMedicalCenterinSanFranciscoandhisresidencyattheUniversityofCalifornia,San
FranciscoMedicalCenter.HesubsequentlyestablishedageneralfamilypracticeinBerkeley,California,wherehe
integratedconventionalmedicalpracticewithalternativemodalities.Dr.Smithscurrentpracticeconsistsprimarily
oftelephoneconsultationswithpatientsaroundtheworld.Hespecializesinapplyingtheprinciplesofmolecular
biologytodifficultdiagnosesanddesigningalternativeandantiagingtreatmentprograms.
AlongtimestudentandadvocateofChineseTraditionalMedicine,Dr.Smithwasinstrumentalinintroducing
acupuncturetotheAmericanmedicalcommunity.In1972,hefoundedthefirstpubliclyfundedacupuncture
clinicintheUnitedStates.In1979,Dr.becameamemberofthefirstdelegationofAmericanphysicians
practicingChineseTraditionalMedicinetovisitthePeoplesRepublicofChina.Dr.Smithisafoundingmemberof
theAmericanAcademyofMedicalAcupunctureandpastvicepresidentoftheAmericanAcupunctureAssociation.
OutofhisinterestinthebiochemistryofChineseTraditionalherbalmedicinesandrecognizingthatthesame
conceptsthatapplytohealingarealsoeffectiveforprevention,inthemid1980sDr.Smithsfocusshiftedto
clinicalapplicationsofnewresearchdevelopmentsinmolecularbiology,cellbiology,andimmunology.His
emphasisonprescribingnontoxicnutritionalmedicines(vitamins,minerals,aminoacids,herbs,
phytopharmaceuticals,andbioidenticalhormones)signalsashiftinthedominantmedicalparadigm.
Phytomedicineswhichencouragehealingratherthanmaskingsymptomsarerapidlyreplacingpharmacologic
medicines.Basedontheseprinciples,in1998Dr.SmithpublishedRenewal:TheAntiAgingRevolution
(Rodale/St.MartinsPress),a650pagebookpresentingaunifiedtheoryofagingandaprogramofdiet,
supplementation,andexerciseforslowingandreversingtheagingprocessandcreatingoptimumhealth.
Forthepasttenyears,inadditiontohisbusytelephoneconsultationpractice,Dr.Smithsprimaryfocushasbeen
onwritingpopularbooksthattranslatecomplexmedicalconceptsintolanguagethelaypersoncanunderstand.A
recentlycompletedbookwillbepublishedin2010.EntitledOutsmartingtheNumberOneKiller,itexplainshow
tousestateofthearttestingandnaturalmedicinetopreventandreverseatheroscleroticallydrivenheart
attacksandstrokes.TheGcMAFBookrepresentsDr.Smithsmostrecentwritingeffort.
Dr.Smithdoesaerobicgardening,playstheelectricbass,travelswidely,andresidesintheruralRussianRiver
ValleywinecountryjustnorthofSebastopol,California,withhiswife,Dellie,histwodaughters,andtheirtwo
dogsandtwocats.
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References
The GcMAF Book
previoustableofcontents
References
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Adenocarcinomas.ClinicalImmunology,Volume119,Supplement1,2006,PageS96.FOCIS2006Abstract
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activatingfactor(GcMAF)forprostateandbreastcancers.Immunology.Italy:Medmond,Bologna.pp.201204.
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macrophageactivatingfactor(GcMAF)ProcAmerAssocCancerRes,Volume45,2004.Experimentaland
MolecularTherapeutics11:SpecificImmuneMechanismsandCancerVaccines:ClinicalStudiesAbstract#1255
YamamotoN.2002.PreparationofpotentmacrophageactivatingfactorderivedfromclonedvitaminDbinding
proteinanditsdomainandtheirtherapeuticusageforcancer,HIVinfectionandosteopetrosis.U.S.Patent
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Institute,Vol.94,No.17,13111319,September4,2002.
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activatingfactoronEhrlichtumorbearingmice.ProcSocExpBiolMed220:2026.
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D3bindingproteinhasapotentadjuvantactivityforimmunization.ImmunolCellBiol76:237244.
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derivedfromvitaminD3bindingproteinhasapotentadjuvantactivityforimmunization.SpecialFeature.
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NobutoYamamoto,VenkateswaraR.NaraparajuandMasahiroUrade.PrognosticUtilityofSerumaN
AcetylgalactosaminidaseandImmunosuppressionResultedfromDeglycosylationofSerumGcProteininOral
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D3bindingproteinderivedmacrophageactivatingfactor.CancerRes57:21872192.
NobutoYamamoto,VenkateswaraR.Naraparaju,MaryMooreandLawrenceH.Brent.DeglycosylationofSerum
VitaminD3BindingProteinbyalphaNAcetylgalactosaminidaseDetectedinthePlasmaofPatientswithSystemic
LupusErythematosus.ClinicalImmunologyandImmunopathologyVolume82,Issue3,March1997,Pages290
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KorbelikM,NaraparajuVR,andYamamotoN(1997).Macrophagedirectedimmunotherapyasadjuvantto
photodynamictherapyofcancer.BrJCancer75,202207.
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immunosuppressionincancerpatients.CancerRes.,56:28272831,1996.
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Immunol.,157:17441751,1996.
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bindingproteinwithadjuvantactivityforantibodyproduction.MolImmunol33:11571164.
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macrophageactivatingfactoronEhrlichtumorbearingmice.CancerResearchProc.,37:481,1996.
Yamamoto,N.,Naraparaju,V.R.,andSrinivasula,S.M.StructuralmodificationofserumvitaminD1binding
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serumproteinrequiredformacrophageactivationaftertreatmentofperitonealcellswithlysophosphatidylcholine.
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photodynamictherapyofcancer.BrJCancer75,202207.
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GcMAF and Nagalase: two amazing proteins.

The GcMAF Book

Routine Nagalase testing finds cancer early and GcMAF

The GcMAF Book

A Cure for Metastatic Cancer?

The GcMAF Book

Professor Yamamoto and Real Science

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Your Incredible Immune Army

The GcMAF Book

The War on Cancer Inside Us

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Your Immune Cells Versus The Pathogen Army: A

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Your Awesome Macrophage Killing Machine

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Macrophages Need GcMAF to Thrive

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How Your Body Makes GcMAF

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Nagalase: Friend and Foe?

The GcMAF Book

How Nagalase Blocks GcMAF Production

The GcMAF Book

If Cancer Cells Could Talk...

The GcMAF Book

GcMAF and HIV/AIDS

The GcMAF Book

The AMAS TestAn Alternative To Nagalase Testing

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The GcMAF Book

Eradicating the Scourge of Cancer from the Face of the

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RetroDocs and NanoMedicine: A Rant

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Differentiation and Cancer Grading

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The Cancer continuum and the 'Point of no return'

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GcMAF Therapy Guidelines

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Why Not Skip Conventional Cancer Therapies and Just

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Knockoffs, Wannabes, Bootlegs, Counterfeitsand

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About Dr. Nobuto Yamamoto

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The Yamamoto Papers

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About the Author

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