Numrul total de ore (pe semestru) din

planul de nvmnt
63

FIA DISCIPLINEI

Denumirea disciplinei

IMMUNOLOGY

Anul de studiu

II

Semestrul * I/II

Tipul de evaluare final (E / V / C)

Regimul disciplinei (Ob obligatorie, Op opional, F - facultativ)

Ob

Numrul de credite

Total ore de studiu

Total ore pe
100
individual
semestru
Titularul disciplinei
Conf. Dr. Petru Cianga
* Dac disciplina are mai multe semestre de studiu, se completeaz cte o fi pentru
fiecare semestru
Total
C**
S
63
28
Medicina
Facultatea
Total ore din planul de nvmnt

Departamentul
Profilul
Specializarea

63

Stiinte Morfo-Functionale
Medicina
Medicina Generala

** C curs, L activiti de laborator, P proiect

sau lucrri practice

Cunotine minime necesare studenilor pentru parcurgerea noiunilor de curs/stagii/lucrri

practice
Cell and molecular biology, biochemistry, genetics, histology, phisiology, histology, microbiology.
Obiectivele disciplinei
Acquiring concepts regarding:
- antigenicity and immunogenicity
- description of the immune system components
- description of immune humoral and cellular responses
- description of molecules within the immunoglobulin superfamily
- description of hypersensitivities
- description of fundamental concepts of autoimmune processes and immunodeficiencies
Programa analitic a cursului
1.Terminology
1.1 Important events in immunology development
1.2 Immunologic memory and vaccination
1.3 Instructive theories
1.4 Selective theories
1.5 General features of innate immunity
1.6 General features of adaptive immunity
1.7 Functionality of the immune system

Nr. ore
2 hours

E1
6
163

L
35

2.Immunogenicity and antigen structure

2.1 Characteristics of antigenic determinants recognized by antibodies
2.2 Characteristics of antigenic determinants recognized by T cells
2.3 The relationship between an antigenic determinant recognized by a Th
lymphocyte and an antigenic determinant recognized by a B lymphocyte ,
found on a proteic antigenic structure
2.4 Factors and conditions that influence the immunogenicity
2.5 Specificity and cross-reactivity; Clinical applications of cross-reactivity
3. Innate immunity natural barriers, cells and molecules
3.1 Natural barriers: anatomical and physiological elements
3.2 Phagocytosis; Endocytosis; Pinocytosis
3.3 Cells and receptors that promote phagocytosis; the concepts of PAMP
and PRR
3.4 NK cell and non-specific extracellular cytotoxicity
3.5 The eosinophil and its role in immunity
3.6 Macrophages, APCs and their role in antigen processing and presentation
3.7 Mast cells their physiologic role and their involvement in pathology
3.8 Interferons
3.9 Acute phase proteins
3.10 Receptors of the innate immune system
4. Receptors for antigen
4.1 Receptor for antigen of B cell; Immunoglobulins
4.2 Antibody structure
4.3 Immunoglobulinic domain
4.4 Variable regions
4.5 Structure and role of constant regions
4.6 Functional properties of immunoglobulin classes
4.7 Receptor for antigen of T cell;
4.8 TCR ; CD3 complex-structure
4.9 Co-receptor molecules CD4 and CD8
4.10 Mechanisms of antigenic recognition of antigen and MHC by T cell
5. Genetics of antigen receptor
5.1 Light chain genes
5.2 Light chain genes
5.3 Heavy chain genes
5.4 Sources of diversity in immunoglobulin generation
5.5 Regulatory mechanisms in immunoglobulin gene expression
5.6 Isotype (class) switch
5.7 TCR gene organization; mechanisms involved in TCR diversity
5.8 Regulation of TCR gene rearrangement
5.9 Secondary type of receptor CD3

2 hours

hours

2 hours

2 hours

6. Ontogeny of B and T lymphocyte

6.1 Primary lymphoid organs: thymus and bone marrow
6.2 Ontogeny of T lymphocyte
6.3 Mechanisms that induce self tolerance for T cell lymphocyte
6.4 Subsets of T cells
6.5 Ontogeny of B cell and stromal cell role in B cell ontogeny
6.6 Mechanisms that induce self tolerance in B cell lymphocyte
6.7 CD5+ B cells
6.8 Malignancies that involve B cells
6.9 T lymphocytes
7 Major Histocompatibility Complex (HLA)
7.1 Cellular distribution of MHCI and MHCII
7.2 Histocompatibility genetics; sources of diversity in MHC; genic map
7.3 Characteristics and functions of MHC
7.4 HLA complex
7.5 Crystallographic structure of MHC molecules
7.6 Clinical importance of HLA phenotyping
8 Antigen processing and presentation
8.1 Antigen presenting cells
8.2 Antigen recognition and immune response
8.3 Identification and characterization of natural peptides associated with
MHCI and MHCII
8.4 Peptides as parts in molecular structure of MHCI and MHCII
8.5 Synthesis and intracellular transport of MHC molecules
8.6 Sites of peptide generation and association with MHC
8.7 General features about pathways of MHC presentation
8.8 Biological consequences of simultaneous recognition MHC-peptide by T
cell
9 B and T cell activation
9.1 Experimental study models for T cell activation
9.2 Requirements for Tcell activation initiation; co-stimulatory molecules
9.3 TCR signal transduction
9.4 Consequences of signal transduction; nuclear transcription factors
9.5 T cell proliferation regulation
9.6 T cell inactivation; Anergy induction
9.7 B cell activation
9.8 Signal transduction by mIg
9.9 Early events stimulation in B cell activation
9.10 Antibody response to Ti antigens
9.11 Antibody response to Td antigen
9.12 Antigen presentation to Th cell
9.13 Th cell signals mediated by cell contact
9.14 Regulation of isotype expression
9.15 Production and B memory cells
9.16 B cell activation versus inactivation

2 hours

2 hours

2 hours

2 hours

10 Functions and mechanisms of action of B and T effector cells

10.1 In vivo functions of T citolytic cells
10.2 CTL and NK cells in bacterial, viral and parasitic infections
10.3 CTL and NK cells in tumoral immunity
10.4 NK cells receptors
10.5 CTLs in autoimmunity
10.6 Interactions lymphocyte-target cell
10.7 Lethal hit and lytic mechanisms induced by lymphocyte
10.8 Auto protective mechanisms of CTL
10.9 Detachment from the conjugate and CTL recycling
10.10 Functional Th populations balance Th1/Th2
10.11 Memory lymphocytes
10.12 Regulatory lymphocytes
11 Cytokines
11.1 The role of cytokines in humoral and cell mediated immunity
11.2 Cytokines families
11.3 Cytokines structure
11.4 Cytokines properties
11.5 Receptor families for cytokines
11.6 Signal transduction through cytokine receptors
11.7 Cross regulation of cytokines
11.8 Cytokines antagonists
11.9 Diseases induced though cytokines
12. Immune response; Inflammation; Lymphocyte traffic
12.1
Lymphoid organs and tissues structure, functions
12.2
Lymphocyte recirculation and homing CD44
12.3 Primary immune response; activation signals
12.4 Germinal center events
12.5 Accessory molecules involved in B-T cell interaction
12.6 Follicular dendritic cells and their involvement in immune response
12.7 Isotype switch and affinity maturation
12.8 Secondary immune response
12.9 Immunological memory
12.10 Adhesion molecules; diapedesis
12.11 Local and systemic acute inflammation; chronic inflammation

2 hours

2 hours

hours

13. Complement system

13.1 Basal circuit of C3; discrimination self-non self
13.2 Alternative pathway of activation
13.3 Classical pathway; immune complexes role in complement activation
13.4 Membrane attack complex; postC3 pathway
13.5 Receptors for complement
13.6.Anaphylatoxins and receptors for anaphylatoxins; role in inflammation
13.7
Complement system regulation
13.8 The role of complement in infections; inflammatory reaction mediated
by complement
13.9 Acute inflammatory reaction mediators and effectors
13.10 Deficiencies of some complement system components; clinical
consequences
14. Deviated immune response: hipersensitivities; types and general
characteristics
14.1 Hipersensitivity type I mechanism, role of IgE and mast cells
14.2 Hipersensitivity type I clinical example
14.3 Hipersensitivity type II- mechanisms of tissue destruction
14.4 Hipersensitivity type II clinical examples
14.5Hipersensitivity type III implications in pathology
14.6Hipersensitivity type IV clinical variants and mechanisms of production
14.7 Hipersensitivity type IV role of T lymphocytes and cytokines produced
by these cells
Programa analitic lucrri practice/stagii
1. Antigenicity/immunogenicity: implications for the immunization process
2,5 ore
2. Crossreactivity : practical applications 2,5 ore

2 hours

2 hours

2,5 hours
2,5 hours

3. Classes and subclasses of immunoglobulines 2,5 ore

2,5 hours

4. Blood groups. ABO and Rh systems. 2,5 ore

2,5 hours

5. Ag-Ab interaction in agglutination reaction 2,5 ore

2,5 hours

6. Ag-Ab interaction in precipitation reaction

2,5 hours

7. Hibridoma, monoclonal antibodies

2,5 hours

8. Solid phase tests: RIA, RIST, RAST

2,5 hours

9. Solid phase tests: ELISA 2,5 ore

2,5 hours

10. Imunohistochemistry, imunofluorescence.

2,5 hours

11. Blot: Western blotting, Southern blotting.

2,5 hours

12. Flow-cytometry

2,5 hours

13. Cellular functionality tests

2,5 hours

14. Immunological investigation in immune pathology

2,5 hours

Competene practice dobndite la finalul anului/semestrului

-

understanding the principles of immunological methods and their application in practice

La stabilirea notei finale se iau n considerare

- rspunsurile la examen / colocviu (evaluarea final):

Ponderea n notare exprimat n

procente
(Total = 100%)
50%

- rspunsurile finale la lucrrile practice de laborator

35%

- testarea periodic prin lucrri de control

15%

- activitile gen teme / referate / eseuri / traduceri /

proiecte etc.
- alte activiti (PRECIZAI) : colocviu
Descriei modalitatea practic de evaluare final E/V (de exemplu: lucrare scris (descriptiv i / sau
test gril i / sau probleme etc.), examinare oral, colocviu individual ori n grup, proiect, etc.)
- oral examination
- MCQ (50 questions)

Estimai timpul total (ore pe semestru) al activitilor de studiu individual pretinse

studentului
(completai cu 0 activitile care nu sunt cerute)
14
1. Descifrarea i studiul notielor de
8. Pregtire prezentri orale
0
curs
2. Studiu dup manual, suport de
28
9. Pregtire examinare final
20
curs
3. Studiul bibliografiei minimale
24
10. Consultaii
indicate
4. Documentare suplimentar n
0
11. Documentare pe teren
0
bibliotec
5. Activitate specific de pregtire
14
12. Documentare pe Internet
0
SEMINAR i / sau LABORATOR
6. Realizare teme, referate, eseuri,
0
13. Alte activiti ....
0
traduceri, etc.
7. Pregtire lucrri de control
0
14. Alte activiti ....
0
TOTAL ore studiu individual (pe semestru) =
minimum 100 ore
-

Bibliografie obligatorie:
Tak Mak, Mary E. Saunders: Primer to the Immune Response, 2011, Academic Press,
Elsevier
Bibliografie suplimentar pentru materia de curs i lucrri practice Imunologie
Richard Goldsby, Thomas J. Kindt, Barbara Osborne : Kubys Immunology, 5th
Edition, 2002;

Charles A. Janeway, Paul Travers, Mark Walport, J. Donald Capra: Immunobiology,

6th Edition, 2004;
David Male, Jonathan Brostoff, David B. Roth, Ivan Roitt: Immunology, 7th Edition,
2006;
Abul K. Abbas, Andrew H. Lichtmann: Cellular and Molecular Immunology, 5th
Edition, Updated edition, 2005

Data completrii:
2012

Semntura coordonatorului de activitate didactic