Code No: RR322306 Set No.

1
III B.Tech II Semester Supplimentary Examinations, Apr/May 2008
PROTEIN ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Write short notes on:

(a) α -helix
(b) β -sheets [8+8]

2. (a) Describe the tertiary structures of protein?

(b) Describe three important forces that are involved in stability of tertiary struc-
ture. [8+8]

3. Describe Posttranslational modification in collagen. [16]

4. Explain in detail about the different Transcription factors that contains Zinc as an
essential element of their DNA binding domains? [16]

5. (a) What are proteases.

(b) Describe different classes of proteases with examples.
(c) Explain the action of serine proteases in details. [5+6+5]

6. Explain in detail Immunoglobulin domains? [16]

7. Write about Pattern databases for storing information about protein families and
motifs? [16]

8. (a) What do you mean by protein design?

(b) What are the goals of protein design? [8+8]

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Code No: RR322306 Set No. 2
III B.Tech II Semester Supplimentary Examinations, Apr/May 2008
PROTEIN ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Describe the assembly of α− helix, with examples.

(b) Describe the geometry of the peptide bond in details. [8+8]

2. Describe the role of hydrophobic forces in tertiary structure. [16]

3. Justify the statement - “Information of final structure of protein lies in its primary
structure”. [16]

4. Describe the zinc finger and their function. [16]

5. Write a note on interactions of proteins in membrane with a neat sketch showing

various classes of proteins associated with the lipid bi-layer? [16]

6. Write in detail about basic structure and function of Immunoglobulin? [16]

7. Describe the advantages and disadvantages of oligonucleotide - directed mutagenesis

using PCR. [16]

8. Explain the importance of protein designing in the field of Drug designing. [16]

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Code No: RR322306 Set No. 3
III B.Tech II Semester Supplimentary Examinations, Apr/May 2008
PROTEIN ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write short notes on:

(a) Parallel β sheets

(b) Anti parallel β sheets [8+8]

2. What are the factors that affect the protein stability? [16]

3. Justify the statement - “Information of final structure of protein lies in its primary
structure”. [16]

4. Describe the structure and function of Leucine zipper. [16]

5. How can you classify membrane proteins? Explain about molecular organization
of Membrane proteins? [16]

6. Discuss the structure of Insulin and describe its functions. [16]

7. Describe the following

(a) Scop − Structural classification of proteins

(b) CATH − Class, Architecture, Topology and Homology data bases. [8+8]

8. Explain the importance of protein designing in the field of Drug designing. [16]

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Code No: RR322306 Set No. 4
III B.Tech II Semester Supplimentary Examinations, Apr/May 2008
PROTEIN ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write down the structure for the following amino acids

(a) Glycine
(b) Alanine
(c) Tyrosine
(d) Tryptophan
(e) Histidine
(f) Phenyl alanine
(g) Cysteine
(h) Iso-leucine [2×8]

2. Describe the role of hydrogen bonds in tertiary structure of protein. [16]

3. Define post-translational Modification? Explain Post-translational modification by

alteration of chain termini? [16]

4. Explain in detail about the different Transcription factors that contains Zinc as an
essential element of their DNA binding domains? [16]

5. (a) What are proteases.

(b) Describe different classes of proteases with examples.
(c) Explain the action of serine proteases in details. [5+6+5]

6. Explain the make up of photosynthetic center and their action with respect to their
structure. [16]

7. Write about Pattern databases for storing information about protein families and
motifs? [16]

8. Describe the basic principles with respect to protein design. [16]

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