20101843-46

CHINA VEGETABLES

100097
063002

3 480.9 molkg-1
FW 318.8 molkg-1FW 213.9 molkg-1
FW 3 44 % 5 4-
-3-4--3-4--3-
-3- 4--3-6 4-
-3- 70.5 %87.5 %
HPLC
S631.1

1000-6346201018-0043-04

Glucosinolate Composition and Content Analysis of Different Radish

Raphanus sativus L.Varieties
1

ZHANG Li , HE Hong-ju , CHENG Jing-hua , ZHAO Xue-zhi

1Vegetable Research Center, Beijing Municipal Academy of Agriculture and Forestry, Beijing 100097, China;
2

Tangshan Vocational Technology School, Tangshan 063002, Hebei, China

AbstractGlucosinolates composition and contents were evaluated taking 6 radishRaphanus

sativus L.cultivars as experiment materials. The result indicates that there are great differences among
total glucosinolates contents of different radish cultivars.Jinghong No.3, an autumn-red radish, has
-1

the highest total glucosinolate content 480.9 molkg FW, followed byJingyanshuiguoxingluobo
-1

-1

318.8 molkg FW. The lowest concent is found inMantanghong213.9 molkg FW,
taking 44 % ofJinghong No.3content. After analyzing glucosinolates composition, we find 5 types of
radish glucosinolates, including 4-methylsulfinyl-3-butenyl-glucosinolates, 4-hydroxyindol-3methyl-glucosinolates, 4-methylthio-3-butenyl-glucosinolates, indol-3-methyl-glucosinolates and
4-methoxyindol-3-methyl-glucosinolates. The predominant glucosinolate in 6 radish varieties is
4-methylsulfinyl-3-butenyl-glucosinolate representing, taking about 70.5 %-87.5 % of the total
amount.

Key wordsRadish; Variety; Glucosinolates; HPLC

2010-05-102010-07-19
2008BADB1B03
E-mailzhangli@nercv.org

www.cnveg.org

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CHINA VEGETABLES

2010 9

glucosinolatesGSL
R 3
myrosinaseisothiocyanates
Mithen2001

80 Mithen et al.2003Sarikamis et
al.2006Raphanus sativus L.
2 000

1.1

6 2 3

3 4 1 1 3
4 1

2009 8 7
2

6 m 40 25 cm 50 cm3 11 6

1.2

1.2.1

0.2 g

15 mL 0.25 mL 5 mL 100 %3 000

-1

rmin 10 min 70 % 5 mL
-1

2 mL DEAE 0.02 molL 2

mL 75 L 2
0.5 mL 3 0.45 m HPLC
2002
1.2.2

HPLC

Waters M820

510 717

1
/min

A/%

B/%

2487 229 nm

100

Novapak C18 20 L

100

21

100

26

100

31

100

A0.05 % B0.05 %
=4 V1 V

-1

1 mLmin 1 31
min

-1

molkg FW
=

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201018

2.1

45

1
3
-1

480.9 molkg FW
318.8 molkg

-1

F W 2 1 3 . 9
-1

molkg FW 3 44 %
3
4 266.3 molkg

-1

FW 1 257.6 mol kg

-1

FW

2.2

A 3 B 4 C 1 DE
F

15
Mithen2001 6 5
2 2 5 6

HPLC
2

4--3-

Glucoraphenin

4--3-

4-hydroxyglucobrassicin

4--3-

Glucoraphasatin

-3-

Glucobrassicin

4--3-

4-methoxyglucobrassicin

2.3

5 3 4--3-

70.5 %87.7 %
4 4--3-

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CHINA VEGETABLES

2010 9

-3- 4--3- 10.0 molkg

-1

FW
3

5
-1

/molkg FW

4--3-

50.60.4

29.21.5

1
47.73.7

49.70.6

4--3-

4.60.6

3.00.6

3.90.7

2.50.2

4--3-

421.036.4

226.311.3

199.938.5

155.013.1

57.26.9

64.95.2

3.20.9
159.62.4

6.50.9
235.013.9

-3-

1.50.4

5.71.1

2.71.3

4.60.7

3.71.2

8.50.6

4--3-

3.11.9

2.00.6

3.50.9

2.20.8

2.60.5

4.00.6

6 5 4--3-

4--3-4--3-
-3- 4--3- 5
20082008
8 2--3- 64--3-
70 %

Schnitzler W H2002352192-197.
20083581205-1208
Mithen R2001Glucosinolates-biochemistrygenetics and biological activityPlant Growth Regulation3491-103.
Mithen RFaulkner KMagrath RRose PWilliamsom GMarquez J2003Development of isothiocyanate-enriched broccoliand its enhanced
ability to induce phase 2 detoxification enzymes in mammalian cellsTheor Appl Genet106727-734.
Sarikamis GMarquez JMacCormack RRichard NBennettRobert JMithen R2006High glucosinolate broccolia delivery system for
sulforaphaneMol Breeding18219-228.

7 33 44 552

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010-82109550

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