Escolar Documentos
Profissional Documentos
Cultura Documentos
DOI 10.1007/s10592-008-9630-1
TECHNICAL NOTE
Received: 20 May 2008 / Accepted: 15 June 2008 / Published online: 4 July 2008
Springer Science+Business Media B.V. 2008
123
722
Primer sequence
Ta (C)
Repeat motif
Boes-3
F50 AAACCTGTCGTGCCAGC30
58
(ATG)23
60
No. of
alleles
Size range
(bp)
PIC
Gene bank
accession no.
140180
0.8
EE-661530
(TAA)8
240280
0.07
EE-661510
60
(CTCG)12
310350
0.33
EE-661498
R50 ATTACCGCCTTTGAGTGAG30
Boes-4
F50 GCGAATGAGAGACCTGCA30
0
R5 CTCGCATAAGCCACATCTG3
Boes-5
F50 ACGGTCGGGAACTTGAAG30
R50 CTCTCGCATAAGCCACATCT30
Boes-6
F50 GGAGGAGACTGAATGATGAC30
R50 GTTTACCACTTCCTTTGCG30
58
(CGG)29
10
210250
0.68
EE-661496
Boes-7
F50 GCCCCAACTGATCCCAAT30
62
(CGG)29
280300
0.48
EE-661496
62
(TG)12
9095
0.53
EE-661488
57
(CTGTG)5
200260
0.71
EE-661477; EE-661555
57
(TC)16
300340
0.84
EE-661410
61
(GA)23
240260
0.80
EE-661366
58
(GA)85
150170
0.77
EE-661316
R50 GCAAACCAAGCTCACATGC30
Boes-8
F50 GCATGTGAGCTTGGTTTGC30
R50 CAACGGTACATCAGTTCACCA30
Boes-10
F50 GCAATAGCATAGCACCAAC30
0
R5 CAAGAACAGAGATGGAGCAG3
Boes-11
F50 GGTACTGTTGTTGAGGAAGG30
R50 TCCCACAACTTCATTCTGC30
Boes-12
F50 GGCAAACAACGGTACATCA30
R50 AGTTCATCTCCCGACATGC30
Boes-13
F50 ACTGATCCCTTGTGTGTACG30
0
R5 GTTCATCTCCCGACATGC3
123
Bamboo Species
Dendrocalamus hamiltonii
Dendrocalamus asper
Dendrocalamus giganteus
Dendrocalamus strictus
Dendrocalamus hookeri
Dendrocalamus
membranaceous
Dendrocalamus longispathus
Bambusa bambos
Bambusa polymorpha
Bambusa balcooa
Bambusa nutans
Bambusa vulgaris
Bambusa multiplex
Bambusa ventricosa
Bambusa nana
Bambusa tulda
Bambusa pallida
Phyllostachys nigra
Phyllostachys aurea
Phyllostachys pubescens
Phyllostachys heteroclada
Phyllostachys bambusoides
Ochlandra travancorica
Ochlandra scriptoria
Melocanna baccifera
S.No.
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Boes-3
Boes-4
Name of locus
Boes-5
Boes-6
Boes-7
Boes-8
Boes10
Boes11
Boes12
Boes13
70
80
80
40
70
30
100
70
100
80
70
90
80
80
70
90
70
80
90
100
80
70
80
80
100
Transferability
(%)
Table 2 Amplification pattern across 25 bamboo species utilized in this study, (+) indicates clear amplification and (-) indicates no amplification/ambiguous amplification or null allele
123
724
Acknowledgement We gratefully acknowledge Department of
Biotechnology (DBT), Ministry of Science and Technology,
Government of India, New Delhi for financial support.
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