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Explain the effect of the DELETION OF the following sequences or features on mRNA.
5 cap (4 marks)
The deletion of the 5 cap would most likely prevent splicing of the
intron that is nearest to the 5 cap. Ultimately, elimination of the
cap will affect the stability of the pre-mRNA as well as its ability to
be translated.
2.
Fill in the blanks and give the functions for each protein factors involved during protein
synthesis in prokaryotes and eukaryotes. (9 marks)
Protein factors
(1 mark each)
Prokaryotes
IF1
Eukaryotes
eIF1A
IF2
eIF4A
EF-Tu
eIF1a
3.
Secreted proteins typically interact with two membrane-bound organelles prior to secretion.
Describe the activities of the two organelles in terms of preparing the polypeptide for secretion out of
the cell.
4. How much energy is required to translate a protein that is 20 amino acids long? Include in your
analysis the amount of energy to add the amino acids to the tRNAs, and all of the steps in
translation. Show your work for any partial credit.
Termination
Total
energy
: GTP GDP + Pi
:
:
:
1=1
80
(6 marks)