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DOI 10.1007/s10529-013-1196-4
REVIEW
Received: 20 February 2013 / Accepted: 20 March 2013 / Published online: 1 April 2013
Springer Science+Business Media Dordrecht 2013
Introduction
The anaerobic ammonium oxidation (anammox) process is the oxidization of ammonium to N2 in the
absence of O2 with nitrite as the electron acceptor.
Unlike other microbial processes involved in the
nitrogen cycle, such as nitrification and denitrification,
research on the anammox process was directed to
improve nitrogen removal from wastewater very
shortly after it was first discovered in a denitrifying
pilot plant in the late 1980s (Jetten et al. 1997; Mulder
et al. 1995). This was due to the perceived potential of
the anammox process for the wastewater treatment
industry (Jetten et al. 1997; Mulder et al. 1995; Strous
et al. 1999; van Dongen et al. 2001).
Nevertheless, the application of this process for
nitrogen removal was not straightforward. The first
obstacle encountered for the laboratory-scale and
industrial application of the anammox process was
the slow growth rate of the responsible microbial
community (Jetten et al. 2009; Strous et al. 1998). In
the laboratory, this hurdle was overcome by using
systems with very efficient biomass retention such as
sequencing batch reactors (SBR, Strous et al. 1998).
At industrial scale, the first full-scale anammox reactor
was started for the treatment of rejection water at the
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Table 1 Process options and names for nitrogen removal systems involving the anammox process
Biomass type
Number of
reactors
Names
Nitrogen removal
rate (kg-N m-3 d-1)k
Reference
Suspended
0.5*
0.26
NAS
CANONc
1.2*
SHARONd,e-anammox
0.6l*
Hybrid
DEMONf
0.6*
Wett (2007)
Biofilm
ANITA-Moxg
1.1
Granular
DeAmmon
0.30.4*
OLANDi
0.05
Aerobic deammonification
1.23m
0.310.45
1
a
SNAP
Stable high rate ammonium removal over nitrite; the name only refers to nitritation where nitrite oxidation is avoided by choice of
residence time and operation at elevated temperature
Commercial name used by Veolia for 1-stage nitritation-anammox in moving bed biofilm reactor (MBBR)
Commercial name used by Purac for 1-stage nitritation-anammox in moving bed biofilm reactor (MBBR)
Single-stage nitrogen removal using the anammox and partial nitritation; name only refers to the process on a biofilm surface layer
Data is for full-scale application [when available and denoted by (*)] or lab-scale installations
Only the aerobic HRT is included in the rate calculations (van der Star et al. 2007; Kampschreur et al. 2008)
Nitrogen removal rate expressed as g N m-2 d-1 (calculated from reported data)
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Process stability
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Concluding remarks
Even though the application of the anammox process
is currently limited to ammonium removal from high
strength wastewaters, results emerging from a multitude of laboratory-scale bioreactor experiments indicate that one of the most intriguing bacteria of the
nitrogen cycle may be applied in the future in several
different ways. For the successful application of each
of these laboratory-scale observations described
above, further feasibility studies are necessary. Nevertheless, each of them represents an unexploited
potential in anammox-based wastewater treatment
systems and they underline the fact that studying
physiology of microorganisms also help to design
better wastewater treatment plants while the quest for
more sustainable treatment systems is a major impetus
to study microbial physiology (Furukawa et al. 2006;
Hippen et al. 1997; Kuai and Verstraete 1998).
Acknowledgments The authors like to thank the many
coworkers and funding agencies (ALW, STW, EU, STOWA,
Paques BV, Agentschap NL) that made this work possible. B.K.,
is supported by the Netherlands Organization for Scientific
Research grant (VENI, 863.11.003).
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