Biology 215-2 Genetics and Molecular Biology

Spring 2016
Instructor: C. Petersen

Genetics is the study of inheritance, and molecular biology reveals the underlying mechanism of information storage, replication and realization. Discoveries in these areas continue to elucidate the
molecular basis for life, have enabled the development of novel biotechnologies, and can identify the cause of, as well as suggest rational therapies for, human disease. The methods and logic we will
examine provide the basic toolset used to study modern biology.

Date

29-Mar
30-Mar

Lecture topic

Reading Griffiths 10th edition (flower cover)

IGA 27-34

1-Apr
4-Apr
6-Apr
8-Apr
11-Apr

Mendels second law: Independent assortment

Recombination, Linkage and gene mapping
Gene mapping II: SNPs

IGA 81-96
IGA 121-132
IGA 132-145 (not 4.4)

Complementation
Gene interaction: biosynthetic pathways
Prokaryotic and eukaryotic model organisms

IGA 219-226
IGA 211-218, 226-233
IGA 759-773

IGA 251-279
IGA 283-306, 359-361

27-Apr
29-Apr
2-May

RNA processing and export

The genetic code
Protein translation

IGA 283-306, 359-361

IGA 309-319
IGA 319-330

4-May
6-May
9-May

Bacterial gene regulation: lac operon

Eukaryotic gene structure and expression
Chromatin and Eukaryotic transcriptional regulation

11-May
13-May
16-May

Review for exam II

Genetic engineering: Recombinant DNA
Genetic engineering: Transgenic organisms

IGA 341-354
IGA 369-377

18-May

Genes in action: Developmental genetics

IGA 449-460

20-May

Genes in action: Cancer genetics

23-May

Stem cells and CRISPR technology

http://www.addgene.org/CRISPR/guide/
http://stemcells.nih.gov/info/basics/pages/basics1.aspx
http://mcgovern.mit.edu/news/videos/genome-editing-with-crispr-cas9/

Neurogenetics

See canvas

Genomics and review for exam III

Memorial Day, no class
Reading week no class

IGA 487-520

3-Jun

June 6th

review session #1
(attendance is optional)
assignment #1 due 4pm
review session #2
(attendance is optional)
assignment #2 due 4pm
7:30-9:30 PM: First
Midterm Exam

Review for exam I

DNA replication
RNA transcription

27-May
30-May
1-Jun

Concept inventory
(participation credit)

IGA 35-38, 40-49, 599-606

IGA 50-65

20-Apr
22-Apr
25-Apr

25-May

assignments due

Overview, biomolecules, DNA structure and mutation,

and information flow
IGA 1-26
Mendels first law
The chromosomal basis of gene segregation,
aneuploidy
Sex linkage and Pedigree analysis

13-Apr
15-Apr
18-Apr

7:30-9:30 pm Wednesday
evening

IGA 381-397
IGA 292-298, 415-424
IGA 426-434

review session #3
(attendance is optional)
assignment #3 due 4pm
review session #4
(attendance is optional)
assignment #4 due 4pm
7:30-9:30 PM: Second
Midterm Exam

review session #5
(attendance is optional)

IGA 580-583; https://www.learner.org/courses/biology/textbook/cancer/cancer_1.html

assignment #5 due 4pm

review session #6
(attendance is optional)

assignment #6 due 4pm

Reading week no class

Exams week no class

Note this date for exam 3 awaits confirmation with registrar

7-9PM: Third Midterm

Exam, covering material
after May 14th.

Additional problems are not graded but provide an added relevant opportunity to develop your skills at solving genetics problems. The intent is not to learn formulas for solving a litany of particular
types of problems but rather enable you through practice to learn to apply the concepts of genetics and molecular biology to novel situations. The Griffiths textbook offers this good advice as you
progress towards mastery (p. 66):
Relevant for Exam 1
Chpt. 2: 34, 36, 40, 42, 43, 44, 45, 51, 52, 55, 56, 72, 74
Chpt. 3: 12, 13, 14, 18, 20, 24, 27, 31, 36, 47, 52, 57
Chpt. 4: 12, 13, 14, 20, 21, 22, 34, 42, 49, 57, 58
Chpt. 6: 12, 13, 14, 15, 16, 17, 27, 32, 33, 35, 60, 77
Relevant for Exam 2
Chpt. 7: 19, 21, 23, 25, 26, 27, 28, 29, 30, 31, 32
Chpt. 8: 8, 12, 14, 16, 18, 19, 20, 25, 27
Chpt. 9: 12, 13, 14, 16, 17, 19, 20, 25, 27, 38, 39, 40, 42, 43, 44
Chpt. 11: 9,10,17,18,19,20,21
Chpt 12: 12, 13,14,15,26,27,28,29,34
Relevant for Exam 3
Chpt. 10: 2, 6, 14, 16, 20, 24, 23, 27
Chpt 13: 14, 21, 22
Chpt. 20: 17, 22
TBA
The solved problems at the end of each relevant chapter are also excellent accounts of the problem solving process for particular topics.