Escolar Documentos
Profissional Documentos
Cultura Documentos
Neurosciences
Paris
Orientation
responses to biological odours
in the human newborn. Initial pattern
and postnatal plasticity
Les r6ponses dorientation
aux odeurs biologiques
chez le nouveau-n6
hurnain. itat initial et plasticit
Laboratoire
depsychopbysique
2 Laboratoire
de comportement
sensorielle,
animal,
CNRS
CNRS
EP 618,
universitP
3 Dkpartement
de psycbologie, universiti
de Reims, et laboratoire
41, rue Gay-Lussac,
75006 Paris, France
Lou&Pasteh,
dephysiologie
de psychobiologie
Strasbourg,
67000
Inra,
du developpement,
37380
&ole pratique
: nouveau-n&
comportementale,
humuin.
fcefus,
naissance.
olfaction,
adaptation
liquide
amniotique,
neonatale
colosfrum.
lait maternel,
ABSTRACT
The initialpa.ttern
and development
of odourpreference
was studied
in infants
simultaneously
to amnioticfltrid
(AF) and maternal
lactealsecretion
(L). Fivegroups
of varying
were studied.
Before postnatal
and breast-feeding
experience
(range: S3.2 fee&)
differentiation
ofAF and L was apparent.
AjZer 3 days and 7-12
breast-feeding
preference
for L arised.
The initial
stage (days 1-3)
may reflect fetal acquisition
sensory/motivational
equivalence
of AF and L odours.
The second stage (days
infantsperctption
of change in milk
development
attests to a high plasticity
Key words:
behavioural
human
plasticity,
and
accept&
aprks
r&i&n
le 17 novembre
reprints
exposed
age (range:
I-5 day)
c&y 3, no evidence of
episodes, a rignQ%ant
of AF odour and
4-5)
may reflect
the
increasing
experience
with milk.
This
stage of human
o/facto y development.
newborn,
human
fetus,
olfaction,
birth, neonatal
adaptation
Note prbentte
par Pierre Ruser
Note remise le 15 septembre
1997,
*Correspondence
quality
and
in the initial
amniotic
1997
fluid,
colostrum,
sequential
breast
postnatale
milk,
France
Not&l&
France
L. Marlier
et al.
VERSIONABR&EII?
Chez pluskurs esptces de mammiferes placentaires, la s&ctivitk des rkponses nkonatales envers Ies stimulations olfactives apparair dkpendante
de lexptrience
olfactive fcetale.
Linformation
olfactive acquise in utero conscitue ainsi une
solution de continuitk sensorielle lors de la transition natale.
Une telle continuitC olfactive transnatale a kte sugg&e dans
le cas de lespkce humaine. Des enfants ?I& de 2 j, nourris au
sein, nexpriment
pas de reponse dorientation
dphalique
diffkrentielle dans un test de double-choix
olfactif prtsentant
simultan&ment lodeur de leur liquide amniotique et lodeur
du colostrum maternel. Cette reponse indiffkrencide
a &T
interprkke
en termes de similaritk chimiosensorielle
et/au
motivationnelle
des deux fluides pCrinatals ; cette similarit&
rtsulterait du transfert simultane des ar6mes de Ialimentation maternelle dans les compartiments
fcetal et mammaire.
Cette etude ;apour but dexaminer deux prtdictions qui d&ivent de lhypothkse de continuitk chimiosensorielie
transnatale :
i) Le de@ (de similarice chimiosensorielle
des deux fluides
devrait Ctre maximal peu aprks la naissance, lorsquils sont
soumis g linfluence simultanee du profil aromatique de la
dikte maternelle. Cette similarit
plus marquke entre les
odeurs amniotique
et colostrale devrait alors rksulter en
labsence de prefkrence relative chez des nouveau-n&
ayant
une expkrience nulle ou trts limit&e de la t&e.
ii) Le traitement olfactif initialement
indiffbrencik des deux
fluides pkrinatals devrait cependant &re rapidement mod&C
en fonction de l%ge et de lexpkrience de t&e au sein ; en
particulier, la direction des reponses devrait Ctre mod&&e B la
fin de Ia phase colostrale, lorsque la qualiti aromatique des
s&r&ions la&es est radicalement modifiee du fait de linstallation des mkanismes galactopokkiques.
Ces deux hypothkses sont examinkes dans une Crude transversale de nouveau-n& Sg& de 5 h B 5 j et dont lexposition au
lait varie de 0 & 32 t&es.
Cinq groupes de nouveau-n&
n&s B terme (n total = 66),
equilibrks selon le sexe, sont exposts aux tests de doublechoix olfactif. Leurs caractkristiques d%ge et dexpbrience alimentaire sont prksentkes dans le tableau I. Ces sujets ont et6
exposCs d des tests de 2 min opposant des tampons de gaze
imprdgnks soit de 20 gouttes de leur liquide amniotique
(AF), soit dune quantitC tquivalente des sCcr&ions 1actCes
maternelles (L). Ces stimulations sont prksenttes de part et
dautre du visage de lenfant, disposkes de faGon symktrique
avec une excentricite de 20-70, et B une distance de l-2 cm
prkvenant tout contact. Mn de pallier les effets potentiels
dune 1atCralisation motrice ou sensorielle, chaque sujet est
tgalement expose aux deux stimulations g droite et a gauche.
Les tests sont video-enregistrks
et lanalyse des rkponses est
r&Me
par un dkcodeur aveugle quant au c&C de prksentation des stimulations.
Les dukes dorientation
vers lun et
iautre stimulus sont mesukes a laide du logiciel Observer.
Le crittre dorientation
vers un stimulus est atteint lorsquun
1000
sujer positionne son nez au-dessus du stimulus, ce qui n&essite une dCviation minimale de 20 par rapport au plan sagittal. La duree dorientation
vers un stimulus est donnee
comme la proportion
moyenne du temps dorientation
vers
ce stimulus en fonction du temps total dorientation
(120 s.).
Le d&ours temporel des rkponses dorientation
relative dans
les tests de choix est present& dam la$pre
1. Une analyse de
variance a deux facteurs (nature de Iodeur et groupe dSge/
experience de t&t&e) rkvkle des effets principaux de lodeur
@ < 0,000 1) et du groupe (p < O,OOl), et une interaction
signifkative entre lodeur et le groupe (p < 0,001). Des comparaisons intragroupes indiquent que les durkes dorientation
relative entre AF et L ne different pas dans les groupes 1 ?I 3
(tableau Z). Les groupes 4 et 5 sorientent plus longuement
vers lodeur L que vers lodeur AF (tableau I). Les nouveaun& manifestent une prkfkrence moyenne B lkgard de lodeur
de L aprks 72 h de vie et 7-12 episodes de tCtCes. Des comparaisons intergroupes r&&lent une dtcroissance de la durke
dorientation
vers lodeur AF et, surtout, un accroissement
rkgulier de la duke dorientation
vers lodeur L. Cet accroissement vers Iodeur L est signifkatifentre
les groupes 1 et 2 et
les groupes 4 et 5.
Cette etude met en hidenceun
d&ours temporel biphasique
de lorientation
relative des nouveau-n& vers deux substrats
odorants auxquels ils ont ttt exposks avant ou aprb la naissance. Au tours des jours postnatals 1 B 3, les enfants nexpriment aucune diffkrenciation
sensorielle ou hCdonique
significative des deux stimularions. Cependant, en fonction
de 1Sge et de lexpkrience de tCtke, les enfants manifestent
une attraction plus grande vers lodeur L que vers lodeur AF,
et cette diffkrenciation
intervient de faGon significative aprks
le troisieme jour. Les rtponses progressivement divergentes
vers chacune des deux odeurs peuvent &re expliqu&es par
laction de deux processus synergiques. La premike phase
(jours 1-3 et O-12 tCt&.s) semble refker, dune part, une
rPponse prefdrentielle du nouveau-&
?I legard dune information acquise B lktat fcetal, lodeur du liquide amniotique,
et dautre part, un traitement sensoriel et/au motivationnel
gquivalent des odeurs amniotique
et colostrale. La seconde
phase (jours 4-5 et plus de 12 t&&es) semble Stre associCe B la
perception
nkonatale dune modification
qualitative
de
lodeur du lait ; aprks linstallation
de la lactation, lodeur des
s&r&ions lacttes dhierait
de faGon croissante de celle du
colostrum, rendant la discrimination
plus aisle entre les
odeurs AF et L. Cette skquence de rtponses pref&entielles
sugg&re que les phases Ies plus prkcoces du dCveloppement
olfactif postnatal sont g la fois dependantes des acquisitions
anttnatales et de lexprkience nionatale. Les rCponses du nouveau-nt humain au colostrum pourraient Ctre influendes par
lexposition
g des qualit& chimiosensorielles
similaires in
utero, et elles sajustent au changement progressif des propritt&s chimiosensorielles
du lait qui interviennent
avec la
lactogenkse.
C. R. Acad.
de
Plasticity
Introduction
The
successful
transition
from
the amniotic
with
early
behavioural
developmental
and
to the postam-
cognitive
capabilities
adjustments
[l].
hours
after birth,
they learn
how
ple,
and
to obtain
milk.
Their
obviously
vital
tasks
is rapidly
that
Within
[2]. The
imprints
fetal brain
has
and coordinated
vioural
training
[3]. The experience-based
med in utero
has been shown
to serve
very
first suc:king
episodes
in the rat
postnatal
colostrum.
human
newborn
no or very
limited
sucking
of such very young,
feedthe primal
stage of relative
exposure
Second,
to AF-like
the initially
cues potentially
similar
treatment
carried
in
of both
fluids
is expected
to change
with
both
age and feeding
experience,
in the same time that colostrum
is progressively
transformed
into milk with the advent
of lactogenesis.
These
two
hypotheses
were
examined
in
a
developmental,
cross-sectional
study
involving
breast-fed
already
beha-
plasticity
the success
[3-51.
Thus,
in the
sensory/hedonic
responsiveness
to the odours
of the fluid
they
experienced
just before
birth
and of the fluid
they
will
encounter
when
sucking
at the breast.
The primal
character
of this response
could
not be ascertained
in the
sample
of infants
tested
previously
at 2 days of age [9],
because
their response
may have been contaminated
by
through
the experience
gained
from their earlier
realization.
Thus,
learning
and underlying
neural
plasticity
are
essential
components
of the adaptation
to the postnatal
niche.
However,
the integrative
activity
of the brain is not
initiated
sharply
at birth
been exposed
to sensory
responses
ducted
with newborns
having
experience.
The responsiveness
ing-naive
infants,
should
settle
niotic
niche
requires
numerous
adaptations
in newborn
mammals.
Besides
the well-documented
cardio-vascular,
respiratory
and
gastro-intestinal
adaptations,
they
are
endowed
optimize
of odour
newborns
exposure
forof the
odour
aged from
5 h to 5 days and
to milk
ranged
from
0 to 32
whose
breast
amount
feeds.
of
information
gained
in the amniotic
atmosphere
constitutes a tie of familiarity
bridging
the fetal and the neonatal
niches
in this species.
The relationship
of fetal learning
to
neonatal
adaptation
and learning
has been investigated
in
other
species,
and humans
Sixty-six
healthy
term-born
newborns
were studied.
These
infants
were
exclusively
breast
fed from
birth.
They were
divided
into five groups
according
to their postnatal
ingestive experience
and age at the moment
of the choice
tests.
The number
of subjects,
age and feeding
experience
cha-
Human
suggested
response
including
[9-l I].
mice
[6],
rabbits
[7]
sheep
Subjects
181,
evidence
of perinata]
olfactory
continuity
was
in a recent
report
on the undiscriminative
of 2-day-old
breast-fed
newborns
simultane-
ously
presented
with
the odours
of their
own
amniotic
fluid
(AF) and of their
mothers
colostrum
[9]. This undifferentiated
olfactory
response
has been
hypothesized
to
racteristics
of each group
are given
in table 1. In group
1,
six infants
had no feeding
experience
at all at the moment
of the tests, and the remaining
subjects
had only
limited
reflect
similarity
simultaneous
mother
into
sucking
experience
in the delivery
room.
Mothers
provided informed
written
consent
to let their
infants
participate in the tests. Each subject
was tested
only once.
112,
of both
perinatal
substrates
due to the
transfer
of the aromas
ingested
by the
the
fetal
and
mammary
compartments
131.
If this
forecasts
sensory
partum
chemosensory
continuity
two expectations.
similarity
of AF and
mammary
secretion
hypothesis
around
birth when
both fluids
temporaneous
priming
of the
This greater
AF/colostrum
odour
an
absence
Table
tions
lacteal
of
relative
is correct,
Stimuli
it
Two types
of stimulations
of blood
or meconium
of chemonamed
prebe highest
in
1. The characteristics
(number,
age,
[means
(M) and standart
deviations
secretions.
choice
feeding
(s.d.)l
tests
test.
con-
experience)
when
exposed
of the five
concurrently
groups
Both
types
of newborns
to the odours
Relative
Groups
Age
N subjects
M
s.d.
9.67
Feeding-experience
range
(N feeds)
(hi
used. AF samples
devoid
were collected
at delivery
and immediately
frozen
(-20 C). The collection
of lacteal
secretions
was
carried
out
by manual
expression
of
20 drops
that were
directly
soaked
(without
contact
with
the nipple)
on a gauze
pad within
the 5 min preceding
the
are submitted
to the conmothers
dietary
aromas.
similarity
should
result in
preference
were
staining
range
of stimulations
studied
of their
orientation
were
and their
relative
familiar
amniotic
presented
head-orientation
fluid
and their
duration
(s)
Lacteal
Fluid
Amniotic
Fluid
s.d.
s.d
at room
duramothers
t*
4.12
515
o-1
0.368
0.205
0.239
0.127
1.62
NS
2
3
20
12
33.6
55.42
8.16
8.48
22118
49-72
2-6
7-l
0.382
0.325
0.171
0.141
0.330
0.388
0.187
0.115
0.68
0.99
NS
NS
4
5
16
9
85.06
114.22
7.91
11.79
0.221
0.193
0.148
0.137
0.43 1
0.537
0.136
0.108
3.3
4.6
0.004
0.002
C. R. Acad.
Sci. Paris,
1997. 320,999.1005
Sciences
de
la vie
7>95
96-l
40
/ Life
Sciences
13-19
>20
L. Morlier
et CII
temperature
on
10 x 10
cm
Hartmann,
Chstenois,
France).
substrate
used in the paired
prior
ratings
of the intensity
panel
gauze
pads
(100
% cotton,
[9].
Apparatus
When
arousal
were
installed
at a 25 angle
state
3-4
[I51
was
in a semi-reclining
with the vertical.
obtained,
the
infants
seat which
back was set
Both stimulus
pads were
fastened
on an U-shaped
device
that allowed
us to present them symmetrically
on each side of the infants
face
with an exclentricity
of 20-70
(for more details
about
the
device,
see [lo]).
This device
was then positioned
so that
both stimulus
pads were
equidistant
at l-2
cm from the
possible
trajectory
of the infants
nose. The infants
visual
scene was homogenized
by spreading
a sheet all around
mation
Technologies,
Wageningen,
The
Netherlands)
with an accuracy
of 0.1 s. The total time oriented
to either
stimulus
was obtained
by adding
the different
orientation
durations
recorded
accross
the two consecutive
trials. The
orientation
duration
toward
a given
stimulus
is reported
as the mean
proportion
of time
spent toward
that ss in
function
of the total time of orientation
(120 s) to either
ss
and to either
sfs. Interobserver
agreement
for orientation
durations
to the ss and sfs calculated
by two coders
unaware
of the nature
of the stimuli
significant
(all Spearman
rank-order
cients:
r > 0.95,
n = 20).
presented
was
correlation
highly
coeffi-
Results
A two-way
ANOVA,
with
odour
as the within-subject
fac-
Procedure
After the stiimuli
were
positioned
symmetrically
on each
side of the infants
head,
the experimenter
(who
stood
behind
the infant)
manually
turned
the infants
head
in
odour
x group
interaction
(F(4,61)
= 5.72, P < 0.001).
The
time course
of the relative
response
in the two-choice
test
is presented
in figure I for the different
groups.
Withingroup
comparisons
of relative
orientation
duration
indi-
order
to alternately
orient
their nose to each stimulus
pad
for 5 s. The direction
of this pretest
orientation
was systematically
counterbalanced
for the nature
and lateral
position of the first stimulus
presented.
The infants
head was
then returned
to the sagittal
position
dissipation
of tonic
neck
asymmetry.
feeds within
72 h of life, newborns
display
an average
preference
for the odour
of their mothers
milk when
it is presented
along with the odour
of their own AF.
and released
after
Head
orientation
responses
were
videorecorded
during
a first I-min
trial.
The lateral
position
of the two stimuli
was then reversed
so that the stimulus
presented
from the right side was presented
from the left side, and vice versa. The infants
head
It is notable
that the stimulus
bearing
the lacteal
odour
elicits
steadily
increasing
head-orientation
durations
as a
function
of age/feeding
experience,
while
the orientation
response
toward
AF remains
and
then declines
over the
Between-group
comparisons
orientation
(f (4,61)
so that both
the right
or
subjects.
stimuli
were
from
the left
presented
side both
The videorecorded
tests were
analysed
by a coder
who
was unaware
of the presentation
side of the stimuli.
The
head orientation
durations
to either
stimulus
were
coded
by tracking
the infants
nose tip. The possible
trajectory
of
180
three
that the
angular
newborns
sectors
nose could
on each side
stable
for the first 3 days,
following
2 days (figure
7).
indicate
heterogeneity
of
duration
to AF throughout
the 1-5-day
period
= 3.68,
P < O.Ol),
with a significant
decrease
in
attraction
to AF odour
between
days 2 and 5 (P < 0.05,
post-hoc
Tukey
test). In contrast,
between-group
comparisons on the orientation
duration
to the lacteal
odour
indicate
a significant
increase
in attraction
to this
odour
between
days 1 and 5 (F (4,61)
= 5.7, P < 0.001).
Significant rises in orientation
to the lacteal
odour
are situated
between
group
1 and groups
4 and
5, and between
sector
corresponding
with the stimulus
(labelled
stimulus
sector,
ss), and two sectors
corresponding
with the zones
free of stimulus
(covering
the o-20
and 70-90
angles
group
longer
oriented
toward
one stimulus
than
toward
the
other.
Infants
were defined
as orienting
longer to one stimulus than to the other when
they spent more than 50 % of
the total orientation
time to both stimulus
sectors
turned
.toward
that stimulus.
From groups
1-5,
respectively
2 on
1002
2 and
group
5 (P < 0.05,
The choice
pattern
examined
by comparing
9 (22
(81.25
of
post-hoc
individual
the number
%I, 8 on 20
%) and
Tukey
C. R. Acad.
tests).
infants
was further
of subjects
spending
13 on
16
longer
Plasticity
newborn
0,s
mosensorily
postnatal
0,4
However,
with
both
increasing
age and exposure
to
feeding
cues,
infants
display
greater
attraction
to their
mothers
lacteal
odour
than
to their
own
AF odour.
A
steady
increase
in relative
orientation
length
to lacteal
might
ance
Groups
1. Mean
relative
durations
(i
hedonically
similar
before
and
on
occur
after the 3rd
of relative
response
postnatal
between
day to modify
AF and lacteal
of subjects,
group,
see
tion
P < 0.0 1.
duration:
to the odour
of their
range
of age and
table
I. Intra-group
mothers
intervals
at 5 %
of breast-feeding
infants
of
opposing
simultaneously
an
(amniotic
fluid:
Af; hatched
environment
lacteal
(maternal
of breast-feeding
expedifferences
in orienta-
secretion
than
to AF
odour
(x2 = 14.47,
df = 4, P < 0.01).
The percentage
of
subjects
orienting
more to the lacteal
odour
than to the AF
odour
is not statistically
different
in groups
1, 2 and 3, but
it is between
these groups
and groups
4 and 5 (groups
l4: x2 = 8.36; groups
l-5:
x2 = 8.10; groups
2-4: x2 = 6.22;
groups
2-5:
x2 = 3.07,
x2 = 6.0;
P = 0.07;
in all
cases,
3-4:
0.06;
df = 1).
Discussion
reveals
a biphasic
time
course
of relative
prefe-
rence
of breast-fed
newborns
between
two biologically
relevant
odours
experienced
either
prenatally
or postnatally
in association
with
ingestion.
On
days
l-3,
the
infants
do not show
evidence
of a reliable
differentiation
of both odours.
This outcome
may result from their lack of
ability
to either
i) detect
the low
in biological
fluids,
ii) discriminate
Sci. Paris,
X9-1005
Sciences
de
intensity
them
la vie
/ Life
odours
carried
on chemosenSciences
the balodours.
sequential
evidenced
associated
with
the head-turning
task might
exceed
the newborns
abilities
during
the first 3 days after
birth.
It cannot
be
excluded
that the recording
of less demanding
responses,
such as oral or vocal
activities,
or state changes,
would
bring
about
an earlier
discrimination
ability
of both types
confidence
of five groups
to choice
tests
environment
and an odour
from the postnatal
secretions:
L; black
columns).
study
and/or
3.
Several
processes
may
be involved
in the
development
of relative
preference
behaviour
by the two-choice
test. i) The motor
constraint
x2 tests;
day
odour
can be observed
throughout
the 5-day
period
covered by the present
study,
but it differentiates
significantly
from the attraction
duration
toward
the AF odour
after the
3rd day. Thus, our results suggest
that some special
events
091
C. R. Acad.
1997. 320,
human
096
092
This
in the
013
columns)
lacteal
responses
Figure
of odour
of odours.
expressing
responses
they were
However,
directional
in a much
laid prone
hour-old
newborns
were capable
of
head turning
and focused
crawling
more demanding
situation
(in which
on their
mothers
chest
midline
in
newborns
already
to different
odour
may have to intea novel
odour
to
positively
respond
to it and to overcome
the prenatally
acquired
positive
response
to AF odour.
The above
data
would
suggest
that, on average,
more
than 7-l 2 nursing
episodes
might
be necessary
within
the first 3 days for the
infant to become
sensitized
or familiarized
with the odour
of milk, and to perceive
lacteal
odour
as different
from,
or
more
strongly
reinforcing
than,
AF odour.
However,
one
study
indicates
that newborns
exposed
to an arbitrarily
selected
artificial
odour
in association
with breast feeding
display
evidence
of conditioned
responses
in favour
of
that odour
2 h after only one acquisition
session
[201. Furthermore,
mere
exposure
to an artificial
odour
without
association
attraction
with feeding
has been suggested
to later elicit
responses
[18]. Thus, the accumulation
of expe-
1003
L. Marlier
et al.
rience
with
fully explain
lacteal
odour
in the feeding
context
does not
the relative
preference
for milk odour
emerg-
ing after
progressive
3. iv) An additional
change
in the quality
daly
process
may
of milk which
involve
facilitates
ond
the
a
the differentiation
of its odour
from
that of AF. As transudates
of maternal
plasma,
both fluids
have overlaping
compositions
majorly
influenced
by the aromas
of the
mothers
diet.
Thus,
amniotic
and lacteal
fluids
should
share a high degree
of similarity
during
the initial
colostral
phase,
when
their aromatic
profiles
are elaborated
under
the synchronous
influence
of exogenous
aromas.
This
degree
partum
of similarity
decreases
then during
days, and especially
after day 3 with
221. Accordingly,
and lacteal
[14, 21,
of amniotic
newborns
aged
ative attraction
terms of growing
the
fluids
olfactory
is easier
in
after day 3.
with a control
stimulus
inclicate
that AF odour
does not become
aversive
after day 3, however
[9]. This reduction
may rather
reflect
a process
of conditioned
extinction
of AF odour
associated
cues
with
the decrease
carriecl
in lacteal
sure
to novel
The
cues
progressively
to either
processes
in re-exposure
secretions,
and
in lacteal
divergent
odours
may reveal
involving
learning
to AF-like
odour
repetition
of expo-
odors.
orientation
response
the action
of two
in the perinate.
curves
concurrent
The initial
treatment
of AF and
lacteal
odours.
Acknowledgments:
This work was supported
by grant
(to BS and RI;), and a fellowship
from Danone
Institute
of the clinique
Sainte-Anne,
Strasbourg,
for their help
in the study.
The
sec-
and
quality
and the sharpening
of milk odour
with increasing
exposure
time to it. This sequential
development
attests
for a high level of functional
plasticity
in the initial
stages
of olfactory
development
in the human
perinate,
as was
previously
described
in animal
newborns
[23, 241. It is
worthwhile
to underline
that this notion
of perinatal
olfactory plasticity
is enrooted
in the ability
of the human
fetus
to encode
odour
information
from
its amniotic
environment,
and to retain
and use it transnatally.
Conclusion
This study reveals
that human
newborns
may be anticipatorily
adjusted
to the chemosensory
qualities
of colostrum
through
their
experience
with
similar
qualities
in utero,
and that by age and feeding
experience
they re-adjust
to
the progressive
change
in lacteal
chemosensory
properties occurring
with
lactogenesis
onset.
As in other
mammalian
newborns
[3, 5, 7, 8, 23, 241, human
newborns
may be born with
chemosensory
images
that direct
the
initial
expression
of attraction
or withdrawal
when
they
are confronted
with
biological
odour
stimuli
in the postamniotic
environment.
The procedure
used here does not
allow
us to separate
the relative
contributions
of age and
feeding
experience
in the development
of the positive
response
to lacteal
odours.
An ongoing
longitudinal
study
should
testing
amount
allow
us to separate
the effects
of both factors
same-age
children
differing
in their
spontaneous
of breast-feeding
experience.
in
REFERENC:ES
1. Alberts J.A. 1987. Early learning
and ontogenetic
adaptation.
In: Perinatul Development:
A Psychobiological
Perspective
(Krasnegor
N.A.. Blass E.M.. Hofer M.A., Smotherman
W.S.. eds.),
Academic
Press, Orlando,
11-37
2. Lecanuet
J.P., Fifer W.P.. Krasnegor
N.A., Smotherman
W.P.
(eds.) 1995. Fetal Development: A Psychobiological Perspective.
Lawrence
Erlbaum,
Hillsdale
3. Smotherman
W.P., Robinson
S.R. 1988. The uterus as environment: The ecology
of fetal behavior.
In: Handbook
ofEehuvioru/
Neurobiology, Vol. 9, Developmental Psychobiology and Behavioru/Eco/ogy(Blass E.M.. ed.), Plenum, New York, 149-196
4. Teicher M.H.. Blass EM 1977. First suckling response
in the newborn albino rat: the roles of olfaction
and amniotic
fluid. Science
198,635~636
5. Smotherman
W.P., Robinson
S.R. 1995. Tracing developmental
trajectories
into the prenatal
period.
In: Fetal Development: A
PsychobiologicalPerspective
(Lecanuet
J.P., Fifer W.P., Krasnegor
N.A., Smotherman
W.P., eds.), Lawrence
Erlbaum,
Hillsdale. 15-32
1004
(days 4-5
perception
of lactogenesis
discrimination
attractiveness
of AF odour
is most apparent
Previous
choice
tests contrasting
AF odour
stage
infants
6. Coppola
D.M.. Millar L.C. 1997. Olfaction
in utero: behavioral
studies of the mouse fetus. Behav. Processes 39,53-48
7. Bilko A., Altbticker
V., Hudson R. 1994. Transmission
of food preference
in the rabbit: the means of information
transfer.
Physiol.
Behav. 56,907-912
8. Schaal B.. Orgeur
P., Arnould C. 1995. Chemosensory
preferences in newborn
lambs: prenatal
and perinatal
determinants.
Behuviour 132.352-365
9. Marlier L.. Schaal B.. Soussignan
R. (in press). Neonatal
responsiveness to the odour of amniotic
and lacteal fluids: a test of perinatal chemosensory
continuity
Child Develop.
10. Schaal B.. Marlier L.. Soussignan
R. 1995. Responsiveness
to the
odor of amniotic
fluid in the human
neonate.
Viol. Neonate
67.
397-406
11, Varendi H.. Porter R., Winberg
J. 1996. Attractiveness
of amniotic fluid odor: evidence
of prenatal
learning?
Acta faediatr.
85,
1223-1227
12. Mennella
J.A., Johnson
A., Beauchamp
ingestion
by pregnant
women
alters the odor
Chem. Senses 20,207.209
C. R. Acad.
G.K. 1995.
of amniotic
Garlic
fluid.
Plasticity
13. Mennella
.J.A., Beauchamp
G.K. 1996. The human infants responses to vanilla flavors in human milk and formula.
Infant Behav.
Develop.
19, 13-19
14. Neville M.C. 1995. Lactogenesis
in women:
A cascade
of
events
revealed
by milk composition.
In: Handbook
of Milk
Composition
(Jensen
R.G., ed.), Academic
Press, San Diego,
CA, 07-98
15. Prechtl
H.F.R. 1974. The behavioral
Infant - A review. Bruin Res. 76, 185-2 12
states
of the
newborn
16. Soussignan
R., Schaal B., Marlier
L.. Jiang T. 1997. Facial and
autonomic
responses to biological
and artificial olfatory
stimuli in
human
neonates:
Re-examining
early hedonic
discrimination.
Physiol. Behav. 62, 745-758
17. Steiner J.E. 1979. Human facial expressions
in response to taste
and smell stimulations.
In: Advances
in Child Development,
Vol. 13
(Lipsitt L.P., Reese H.W., eds.), Academic
Press, New York, 257-295
18. Balogh R.D., Porter R.H. 1986. Olfactory
from mere exposure
in human neonates.
9,395-40
1
preferences
infant Behav.
resulting
Develop.
of odour
responses
in the human
newborn
19. Pihet S., Mellier D.. Bullinger A., Schaal B. 1997. Reponses
comportementales
aux odeurs
chez
le nouveau-&
premature:
etude preliminaire.
Enfance
1 /1997,33-46
20. Sullivan R.M. 1990. Newborn
human infants exhibit CRs to an
odor previously
paired with either breast od bottle feeding.
Presented at the Annual Meeting
of Intern. Sot. Devel. Psychobiol.,
Cambridge,
UK
21. Kulski J.K., Harmann
P.E. 1981. Changes
in milk composition
during
initiation
of lactation.
Au&r. J. Exp. Biol. Med.
Sci. 59.
101-l 10
22. Patton
S., Huston GE,
Montgomery
P.A., Josephson
R.V.
1986. Approaches
to the study of colostrum
-The onset of lactation In: Humun
Lactation
2, Maternal
and Environmental
Factors (Hamosh
M., Goldman
AS. eds.), Plenum Press, New York,
231-238
23. Hudson R. 1993. Olfactory
imprinting.
Curr Opin. Neurobiol.
3.
548-552
24. Wilson D.A., Sullivan R.M. 1994. Neurobiology
of associative
learning
in the neonate:Early
olfactory
learning.
Behov. Neural
Biol. 61, l-l
1005