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'"safes-
INTRODUCTION
Airborne infections are caused by bacteria and virus that survive
their transport through the air, find a favorable landing site, and
cause an infection.
A number of
If the
This
A more extensive
plotted as shown later in this report but not put into the technical
paper.
Readings of relative humidity, dust, and airborne counts were
taken before the class started 08:30, then repeated at 09:30, 10:00,
11:00, 11:30, 12:15, 12:40, and 12:35.
Relative humidity was measured with an aspirating psychometer
with readings taken during the microbial sampling period.
The airborne bacteria counts were made with an Andersen sampler
that collects air at 28.3 L/min through six stage with decreasing
diamter holes in each succeeding plate. As the jet velocity increases
with the decreasing diameters of the holes, smaller particles are
recovered at each successive stage.
5-15 minutes, then the petri plates were removed, incubated, and the
colonies counted by the 'positive hole' method.
The reproducibility of the Andersen sampler was confirmed by
using two additional samplers side by side.
monitor was used to determine the respirable mass content of the air.
Mass content of the air was registered in Mg/m
balance.
by a piezoelectric
that value.
The
Five
samples were taken in each test. Analysis of the gas was made with an
infrared analysis instrument.
The combined
Then it became
are dispersed by the human body at a very high rate 5 x 10 cells per
7
day of which 10 particles bear viable organisms. For the same reason
as the schools, an immense number of readings would be required in
To limit the
central air conditioning and the same system ventilated and humidified
the rooms in winter.
radiators.
Hospital B and C were not air conditioned, not humidified, and the
windows and storm windows were openable.
RESULTS SCHOOLS
The results of the school investigation are reported in the paper
"The Effect of Ventilation and Relative Humidity upon Airborne
Bacteria in Schools" attached to this report.
The effects of
discharge rate determined with the room unoccupied is not the correct
value for the occupied state of this classroom.
were done on unoccupied rooms because N 2 0 was used as the tracer gas.)
The trend of the data would suggest there is a relationship between
the number of monthly non-pathogenic cfu/m
measured in this
other pathogens and since they appear in very low numbers, it may be
difficult or impossible with the known methods.
RESULTS HOPITALS
The concentration of non-pathogenic airborne bacteria in the
intensive care units of the hospitals for the period August to
September, 1983, are shown in Figures 3,4 and 5.
also caused excursions from the basic levels. The reduction on the
3
cfu/m with reduction in relative humidity was similar to the schools
and no pathogens were found in the hospitals.
CONCLUSIONS
Schools
The investigation of the effect of relative humidity and
ventilation on the airborne bacterial content of air in schools
revealed the following:
3
1. The number of airborne bacterial colony-forming units per m
of
They may
However, the
confirm
the
reduction
of
10
UTILIZATION OF HP-397
The Effect of Ventilation and Relative Humidity upon Airborne
Bacteria in Schools
., JU-USLE J.
School Heating and Ventilating Systans
p-.
Heating System
Humidifying
System
Outdoor Air
Per Person
Natural Gas
Warm - Air
Air Washer
1.5 L/sec
3 cfm
2.6
15
525
HC
Natural Gas
Warm - Air
Air Washer
1.5 L/sec.
3 cfm
3.0
8.2
287
KG
Steam Radiators
Air Washer
5.0 L/sec
100 cfm
15
525
NP
Hot Water
Radiators
None
1.5 L/sec
3 cfm
3.00
13
455-
QE
Unit Ventilators
None
0.63
13.6
476
Unit Ventilators
None
0.63
15
527
School
Recirculation
Rate Ac/Mr
Room Vol.
Per Student
Unit ventilator controls were set with minimum fresh air when heating was required
opening to admit outdoor air as cooling was required.
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No.2887(RP-397)
K. Sareen
A.D. Osborne
ASHRAE Fellow
ABSTRACT
INTHTPCTIOW
Airborne infections are caused by bacteria and virus that survive their transport through the
air, find a favorable landing site, and cause an infection. Survival depends upon a number of
factors, and this paper investigates two of them, indoor relative humidity and ventilation.
Relative humidity affects the survival of airborne microorganisms, and the general rule seems
to be that the midrange of relative humidity is the most lethal (Akers and Oinmick 1969). A
number of investigators (Sale 1972; Hemes, Kbol, and Winkler; Hope-Simpson 1958) attribute the
increase in respiratory infections in winter to the lower indoor relative humidity of that
season permitting an increased survival of airborne infectious microorganisms. Studies that
have been conducted with a population divided into two groupsone in a higher humidity, not
exceeding 50% BE, the other with a lower humidityhave found the incidence of colds or
absenteeism was reduced in 9 out of 13 experiments with the higher humidity. If the cause of
reduction in colds and absenteeism is the reduced survival of airborne infectious agents in
the high humidity range, then another cause in reduction should occur with increased
ventilation, as the airborne-viable contents of the air will be diluted. This investigation
tries to validate the hypothesis that indoor relative humidity and ventilation affect the
transmission of colds. The airborne microbial count was measured in six schools along with
ventilation or recirculation rates, relative humidity, dust, and absentee rates to determine
if there was a relationship between these factors.
same school.
The mechanism of the death of viable particles, which have been studied in an aerosol
chamber, has shown two slopes: the initial decay, ^ , and a later decay constant, K_. In
this study, a mean decay was determined because of the length of time required for airborne
bacteria sampling.
The loss of viable airborne bacteria in the air is
loss due to the recirculated air rate and the decay loss
the air, which has been shown to be a function of a
relative humidity, which has a significant effect (Akers
The study of the separate effect of air changes per hour and relative humidity was
carried out by determining the slope, K, of the decay curves. In Figure 8, a study of the
effect of air-change rate in a single school at a constant relative humidity is shown. The
test began at 11:55, so the cfu/m are at a maximum; then the students leave, so the occupancy
is zero. Curves A was are obtained by turning off the ventilation fans so that the decay
represents the lowest air-change rate, caused by infiltration only, which was found with
tracer-gas decay equal to, 0.30 AC/hr. -At the same time, samples of air were taken with the
sampler to find the decay rate of cfu/m with no occupancy. This slope is given by Curve A'
equal to 1.80 AC/hr. The loss of viability due to biological death is represented by the
difference in slopes between A and A'. The decay rate was equivalent to 1.5 = (1.8-0.3)
AC/hr. Repeating the test with the ventilation fans on, Curve B is the decay rate represented
by the dilution of the ventilation air and Curves B' are the combined effect of ventilation
and biological death.- The biological decay rate of curve B is 5.62 - 3.0 = 2.62 AC/hr.
The increase in cfu/m
Figure 9.
Figure 10 shows the reduction in the cfu/m that occurred in one school with reduction in
indoor humidity. All the data showed the same trend, that is, a reduction in indoor relative
humidity resulted in a reduction of viable airborne bacteria.
Several species of the non-pathogenic airborne viables from the schools were grown in
culture so they could be atomized into the air of a chamber that could be kept at a selected
relative humidity. Samples taken at time intervals established the viability of the bacteria
at various humidities. The tests showed that the survival time of bacteria decreased with
lowered relative humidity.
Respirable dust levels of 10 micron or less showed a relationship to the cfu/m increased
as the duct content increased as shown in Figure U .
Whether ' the bacteria and their
associated skin cells contributed to the dust count or vice versa is not known.
Absenteeism in the various schools and their average relative humidities and
recirculation rates are shown in Table 6. These are the studies of the Grade 3 classroom in
each school that had about 20 pupils each. There was a small but not significant decrease in
absenteeism with increase in recirculation rate. There was no significant difference in
absenteeism due to the different relative humidities in the various schools. The sample size
of 20 students per class is not large enough for statistically significant results.
An
analyses of the entire population of the schools over several years will have to be undertaken
to establish the effect of relative humidity and recirculation rate on absenteeism in schools.
DISCOSSION
Occupancy is the major factor in determining the number of viable, airborne bacteria {cfu/m )
in occupied spaces.
The high dispersal rate of viables (10 per day per person) was
demonstrated in the rapid rise of cfu/m immediately after the students entered the classroom
and by the rapid decrease of the counts when the students, left the classroom. During the
occupied period, the level of airborne bacteria (cfu/m ) was determined mainly by the
recirculation rate of air supplied to the room.
Dilution with outdoor air (measured outdoor air counts were -3.8 C, 52 c f u / ; 22 C, 26
cfu/m ) where the counts were usually from 25 to 75 cfu/m resulted in only a small reduction
in bacteria because the outdoor air rate was about 10% to 15% of the recirculation rate.
3. Ordinary filters with high air recirculation rates are highly effective in removing
airborne bacteria from rooms. They may constitute a means for protecting building occupants
from bacterial pathogens produced by sprays, humidifiers, and other conditioning equipment
that could produce illness by causing airborne bacteria.
4. A decrease in indoor relative humidity reduced the number of cfu/m in the air.
Other studies that showed the opposite did not take into account the variation in number of
occupants and the effect of ventilation and air recirculation.
5. This study did not substantiate the hypothesis that the reduction in absenteeism and
respiratory illnesses in winter with increased indoor relative humidity is caused by the
reduction in airborne microorganisms with increased humidity.
6. Pathogenic bacteria were only found in the midwinter season.
Vfliile this investigation revealed many aspects of airborne bacterial contamination in
schools that may be significant to all buildings, further investigations will have to be
undertaken to substantiate the findings.
Andersen, A.A., "New Sampler for the Collection, Sizing, and Enumeration of Viable Airborne
Particles," Journal of Bacterilogy, V. 76, 1968, p. 471-84.
Akers, A.B.; and Dimmick, R.L., An Introduction to Experimental Areobiology, Wiley, 1969, p.
280.
Dang, V.B.; Guberan, E.; and Sweetman P.M., "L'humidification de L'air des Locant Previent
elle les Maladies Respiratoires Pendant L'hiver," Schweizerische Medizinische
Wbchenschrift, 108, No. 22, 1978.
Druett, H.A., "The Inhalation and Retention of Particles in the Human Respiratory System,
Airborne Microbes," in P.H. Gregory and J.L. Montieth, eds., 17th Symposium of Society
for General Microbiology, London, Cambridge Univ. Press, 1967.
Green, G.H., "The Positive and Negative Effects of Building Humidification," ASHRAE Trans.,
1982, Vol. 88., Pti 1.
Green, G.H., "Winter Humidities and the Related Absenteeism in Canadian Hospitals," Digest of
the 3rd CMBES Canadian Clinical Engineering Conference, Sept. 1981, Saskatoon,
Saskatchewan.
Green, G.H., "The Effect of Indoor Relative Humidity on Absenteeism and Colds in Schools,"
ASHRAE Transactions, 80, Part 2, 1974.
Gregg, J.B., "The Importance of Environment in Prevention of Upper Respiratory Infections,"
South Dakota Journal of Medicine, 21, 1968, p. 21-25.
Hemmes, H.J.; Kool, S.M.; and Winkler, K.C., "Virus Survival as a Seasonal Factor in Influenza
and Polyiometis," Nature, 188(4748), p. 430.
Hope-Simpson, R.E., "The Epidemiology of Non-Infectious Diseases," Royal Society Health J.,
78, 1958, p. 593.
Kilbourne, E.D., and Schulman, J.L., "Airborne Transmission of Influenza Virus Infection in
Mice," Nature, Sept. 15, 1962, 1129.
Lester, W., "The Influence of Relative Humidity on the Infectivity of Airborne Influenza A
Virus," Journal of Exp. Med., 88, 1948, p. 361-8.
Lubart, J., "The Common cold and Humidity Imbalance," New York State Journal of Medicine,
March 15, 1962, p. 816-819.
Luciano, J.R., "New Concept in French Hospital Operating Room HVAC Systems," ASHRAE Journal,
Feb. 1984, p. 30.
TABLE 2
School Heating and Ventilating Systems
School
Heating System
Humidifying
System
Outdoor Air
Per Person
Natural Gas
Warm - Air
Air Washer
l.S L/sec
3 cfm
2.6
15
525
HC
Natural Gas
Warm - Air
Air Washer
l.S L/sec
3 cfm
3.0
8.2
287
KG
Steam Radiators
Air Washer
5.0 L/sec
100 cfm
15
525
NP
Hot Water
Radiators
None
l.S L/sec
3 cfm
3.00
13
45S
QE
Unit Ventilators
None
0.63
13.6
476
Unit Ventilators
None
0.63
15
527
Recirculation
Rate Ac/Mr
Room Vol.
Per Student
_ 3J r <t-*
m
Unit ventilator controls were set with minimum fresh air when heating was required
opening to admit outdoor air as cooling was required.
TABLE 3
Bacterial Species Isolated
VENTILATION DUCTS APARTMENTS
Airborne bacteria
Micrococcus spp.
SWISS OFFICE
SASKATOON SCHOOLS
C.f.u./m of air
7.28
Micrococcus
Staphylococcus spp.
Staphylococcus aureus
Aeroceceus viridaoa
0.08
2.37
Staphylococcus
Streptococcus
(group D)
S. (group vixidans)
S. spp. (other groups)
0.89
0.S5
1.S4
Streptococcus spp.
3t-eptoccccus
Bacillus cereua
B. Subtilis
B. spp. (other groups)
0.26
0.19
1.17
Bacillus spp.
Bacillus spp.
Coruneoacteriun xerosis
C. ulcerans
C. haeRolueicuff
C. spp. (other groups)
0.25
0.12
0.03
0.10
Corynebacterium SPP>
C. spp.
Enterobacter
Acinetobacter
0.06
0.86
Gratr-negative bacilli
Gram-negative b a c i l l i
agglomrans
caicoaceticus
3.21
Pseudouonas vesicularis
P. spp. (groups CDC)
P. pucre/asciens
P. picketti
P. paucimbilis
P. spp. (other groups)
0.22
0.26
0.07
0.06
0.03
0.06
Mora mils
osloensls
Branhanella cacharrhalis
Flavobacterium weningosepticun
0.34
0.31
0.13
0.31
Neisseria spp.
0.07
Total
21.82 c.f.u./m
aureus
S. Aureus
Neissera spp.
3
spp.
7-
GENEVA
SASKATOON
GENEVA
9ERNE
MISSOURI
6V)
m
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a:
I9T7
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o
OFFICE STAFF S.
***
-*ARMY RECRUITS S.
(OCCURENCES] MISSOURI
IS
20
23
30
35
% RELATIVE
Figure
1.
Effect
of
absenteeism
-1
ss
-r4S
40
SO
HUMIDITY
relative
of
humidity
adults
on
xTUUu
10
-GRADES I TO 3 N.S.
9-
AROMORE P.A.
8-1
NURSERY SCHOOL S.
NORFOLK VA.
GRAOES I TO 8 N.S.
GRADES I TO 6 N.S.
(NO HUMIOIFICATION I
HALIFAX
( NO HUMIOIFICATION )
COPENHAGEN
5
in
in
03
GRADES I TO 8 S.J
<
SASKATOON
KINDERGARTEN S.
ARDMORE P A .
SASKATOON
HALIFAX
BASEL
COPENHAGEN
NORFOLK V A .
BASEL
IS
20
25
30
35
40
45
50
55
% RELATIVE HUMIDITY
Figure
2.
Effect
of relative
humidity
absenteeism
of
children
on
2000
C
HC
KG
NP
QE
W
AIR CHANCES R . H . %
o
a
W
0
o
2.60
2.27
1.87
3.00
0.63
0.63
34
37
32
27
27
25
W-
1500
0.63A.C.25%R.H
l.87A.C.32%R.H.
z
a
z
1000
3.00A.C.27%R.H.
2.60A.C. 34%8.H.
o
8 500
OCCUPIED
i
I
9
10
OCCUPIED
LUNCH
OCCUPIED
I
I
i
II
12
13
TIME
Figure 6.
Airborne microorganisms,
variation
with time in
schools
Figure 7.
in air-handling
60
X
cc
hi
O
<
2800-1
40 -
UNOCCUPIED
OCCUPIED
UJ
CARPETED ROOM:
.'
2400-
20
<
2000-
1000
U.
o
2
o
z
o
o
UJ
CD
<
or
/
/
1400-
600
400
400-
200
/
0
/
800-
'
o /
o / o
-,
u
SEPT.
Figure 10.
U.
on
Ul
/
/
/
/
E
800
L *
1600-
OCT.
NOV.
DEC.
/8
1
!
04
1
12
1
16
DUST mg/m3
JAN.
Figure
II.
1
20