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Agronomy 2: 1 - 7, 2008
©Wilolud Online Journals, 2008.
EFFECT OF METALAXYL AND NEEM SEED OIL ON THE INCIDENCE OF DOWNY MILDEW OF
PEARL MILLET
1
Aliyu, B., 2Hati, S. S., 3Donli, P.O. and 4Anaso, A. B.
1
Department of Biological Sciences, 2Department of Chemistry, Gombe State University, Gombe
3
Department of Biological Sciences, 4Department of Crop Science, University of Maiduguri, Maiduguri
ABSTRACT
Millet is one of the most important staple food crops in Africa and India. The grain
yield losses as a result of downy mildew disease varied from 10 to 50% annually.
This study was aimed at determining the effect of seed treatment with metalaxyl
either alone or in combination with neem seed oil foliar sprays on the incidence of
pearl millet downy mildew. Field trials were carried out in 2006 and 2007 growing
seasons. Split - plot design was employed with four categories of chemical treatments
and three varieties of pearl millet (SOSAT-C88, EX-Borno and GB8735). The
treatments were in triplicates. The results of the study showed that incidence of
downy mildew on vegetative shoots of pearl millet differed significantly (p<0.05)
among the varieties studied. Generally, SOSAT-C88 showed significant resistance to
downy mildew than the other varieties. As anticipated, the combination of metalaxyl
and neem oil spray significantly (p<0.05) reduced the incidence of downy mildew on
pearl millet and improved yield compared to the neem oil spray alone and the control
during the 2006 season. From this study, metalaxyl was found to be still an effective
fungicide against downy mildew in pearl millet. However, supplementing metalaxyl
with neem oil spray generally increased grain yield.
INTRODUCTION
Pearl millet (Pennisetum glaucum (L) R. Br.) is one of the most important staple food crops in Africa and
India (Williams, 1984), and is grown as a subsistent crop in the Sudano-Sahalian Savanna of northern
Nigeria. The grain is nutritious and contains 5-7% oil. It has higher protein level and more mineral
constituent than maize and rice (NRC, 1996). The arable land put to pearl millet in West Africa has been
estimated to be 23.99 million hectares; and in Nigeria it is about 5 million hectares, which places Africa as
the third largest producer of the world’s pearl millet (FAO, 1985). It yields relatively well in regions too
hot and too dry to support good yield of maize and sorghum (NRC, 1996). Millet in Nigeria is
predominantly produced in Bauchi, Borno, Gombe, Kano, Katsina, Sokoto and Yobe States.
Apart from the harsh environmental conditions which limit the crop’s production potentials, pearl millet is
attacked by various diseases and pests in the field. The most important of the fungal diseases of pearl millet
is downy mildew, caused by the fungus (Sclerospora graminicola (Sacc.) Schroet), which has been
estimated to cause losses of 10 – 15% in Nigeria. Other diseases of pearl millet in Nigeria are smut
(Moesziomyces pennicullariae (Bref)) Vankysyn. (Tolysposporium pennissscullariae (Bref)) and ergot
(Claviceps fusiformis (Loveless) (Thakur and King, 1988) while important invertebrate pests include stem
borer (Coniesta ignefusalis), head miner (Raghuva sp), the head beetle (Pacnoda sp) and grasshoppers
while Quelea quelea is the major vertebrate pest and parasitic flowering plant, Striga (Rai and Kumar,
1994).
Yield losses of up to 50% due to downy mildew have been reported from West Africa (Singh et al., 1987).
An annual loss due to downy mildew in Nigeria was put at 10% (King and Webster, 1970). Waller and Ball
(1982) reported consistent disease incidence of 10-15%.
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Aliyu, B et al: Continental J. Agronomy 2: 1 - 7, 2008
Wheeler (1979) reported that downy mildew is controlled chiefly by cultural methods, by spraying
fungicides and by planting less susceptible varieties. It is, however, by the application of fungicides that
the most practical control of this disease had been achieved. Anaso et al. (1989) reported that maize downy
mildew is successfully controlled by seed treatment with metalaxyl and host resistance. Nene and Singh
(1976) noted that two methods of chemical control of pearl millet downy mildew had been attempted, one
was treatment of seed with fungicide to control seed borne inocula and the second method was the use of
foliar sprays to control secondary spread.
Metalaxyl is a known fungicide for seed treatment to control downy mildew, its action is systemic and
contains benzenoid (phenylamide), used in mixtures as a foliar spray for tropical and subtropical crops, as a
soil treatment for control of soil-borne pathogens, and as a seed treatment to control downy mildews
(Kimmel, et al, 1986). Similarly, neem oil, a vegetable oil pressed from the fruits and seeds of neem
(Azadirachta indica), have scientifically been validated for many of its traditional uses to treat bacterial,
fungal, and viral infections, including its use as pesticides (Schmutterer, 2002). The neem tree is found in
abundance in northern Nigeria and the seed’s oil is thought to be very likely to effectively control downy
mildew of pearl millet. The neem seed oil is known to contain nearly 100 protolimonoids, limonoids or
tetranortriterpenoids, pentanortriterpenoids, hexanortriterpenoids and some nonterpenoid constituents.
Different batches contain from 1700 ppm azadirachtin to 2500 ppm azadirachtin as the active ingredients.
The oil contains small amounts of nitrogen, phosphorus, potassium, and other nutrients. It also contains the
following fatty acids - Palmitic acid 19.4%, Stearic acid 21.2%, Oleic acid 42.1%, Linoleic acid 14.9% and
Arachidic acid 1.4% (Conrick and Schmutterer, 2004).
In northern Nigeria, pearl millet is regarded as second only to sorghum in importance. The grain is used in
a variety of traditional foods such as “fura”, “biski”, “masa”, “yartsala”, “tuwo”, “kunu”, “burukutu” and
“pito”. Its production supercedes that of sorghum because of its outstanding ability to withstand drought, its
early maturing advantages over other crops and it is the first grain crop grown by farmers in Northern
Nigeria in anticipation of early grain for consumption when other reserved grain become exhausted
(Nwasike et al., 1983). In view of the decreasing level of production emanating from the constraints
already highlighted, it was therefore considered essential to carry out a study that would assess the
potentials of seed treatment with metalaxyl and neem seed oil either alone or in combination with neem
seed oil as foliar sprays on the incidence of pearl millet downy mildew for the control of this disease.
Neem oil was obtained from extraction according to method described by Kaura et al (1998). Petroleum
ether (bp 60-80°C) was used as extraction solvent, which was recovered from filtrate by ordinary
distillation at 70°C, further in rotary vacuum evaporator placed on a water bath for 20 hours at 60-70°C.
After extraction the neem oil was diluted with different amount of liquid paraffin to obtain different
concentrations of extracts.
Experimental Site
The experiment was conducted during 2006 and 2007 cropping seasons in Maiduguri (Latitude 11051' N;
130 15'E) at the Teaching and Research Farm of the Department of Crop Science, Faculty of Agriculture,
University of Maiduguri.
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Aliyu, B et al: Continental J. Agronomy 2: 1 - 7, 2008
The experimental site is a sandy loam and has been classified as typic ustipsamment. The mean annual
rainfall range between 40 and 250mm with an annual average temperature ranges from 20-43oC. The area
has been cropped with millet for the several years ensuring build up of disease inoculum. Natural
epiphytotic in field was therefore relied upon as the source of inoculum in each season. The split - plot
design was employed in this study.
Chemical Treatments
Chemical treatments of the pear millets were made in four categories:
1. Metalaxyl (0.4g) per 50g millet treatment prior to planting,
2. Metalaxyl (0.4g) per 50g millet treatment prior to planting + neem oil ( 0.3kg foliar spray)
3. Neem oil (0.3kg) foliar spray based on fungal LD50 (Schmutterer, 2002) and
4. Control (neither metalaxyl nor neem seed oil)
The neem oil was dispensed using electrodyn sprayer on non rainy days. Triplicate plots treatments
comprised three varieties namely, SOSAT-C88, Ex-Borno and GB 8735.
The seeds were sown when the rains established at rate of ten seeds per hole and spaced at 75 X 30 cm
between rows and within rows, respectively. At three weeks after sowing during the first weeding plants
were thinned to two plants per stand.
Fertilizers were applied at the recommended rates of 60kg/N/ha, 30kg P2O5/ha and 30kg K2O/ha (Anaso,
1989). At planting, basal dose of NPK (15:15:15) at the rate of 48g N, 48g, P2O5 and 48g K2O were
applied respectively to each plot. The remaining dose of 48gN was applied at six weeks after sowing by
the side placement method. Weeds were controlled manually using hoes on the third and the sixth weeks
after sowing. Harvest was done manually at maturity using cutlass. The plants were cut at their bases and
placed in their respective plots for further drying after which the panicles were cut and threshed.
Parameters Measured
Millet stands in each plot were counted and recorded at 56 days after sowing. Grain yields for all treatments
were obtained after harvesting, sun-drying, threshing and winnowing. They were then weighed on a
Mettler balance (0-10kg) and yields per plot determined. The yields were later converted to kg/ha. The
number and weight of panicles per plot were determined.
Data collected were subjected to analysis of variance (ANOVA), using Microsoft Excel (2003). Variations
of results were considered significant at p<0.05.
RESULTS
The three varieties of pearl millet showed varied responses to downy mildew attack in the area of study.
The incidence of downy mildew on vegetative shoots of pearl millet differed significantly (p<0.05) among
the varieties studied (Table 1). The variety GB8735 showed high incidence compared to SOSAT-C88 and
EX-Borno. The difference between SOSAT-C88 and EX-Born was not significant for the main stems and
significantly different for basal tillers in both years (Table 1).
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Aliyu, B et al: Continental J. Agronomy 2: 1 - 7, 2008
Among the fungicides applied the combination of metalaxyl and neem oil spray and metalaxyl alone
significantly (P<0.05) reduced the incidence of downy mildew on pearl millet main stems compared to the
neem oil spray alone and the control during the 2006 season (Table 1). A similar trend was observed on the
basal tillers except that the difference between the application of neem oil spray and the control was not
significant (Table 1).
The production of panicle and grain yield of pearl millet differed significantly (P<0.05) among the three
varieties (Table 2). The varieties SOSAT-C88 and EX-Borno performed better in production of panicle and
grain yield than GB8735 in the year 2006. in the following year the difference among the varieties was
significant.
Table 1: Effects of variety and fungicide on the incidence of downy mildew on the vegetative shoots of
pearl millet in Maiduguri during the 2006 and 2007 growing seasons
Fungicide
Neem oil spray (NOS) 46.60±6.7 43.88±5.4 51.82±8.3 56.26±10.4
Apron XL-LS 31.04±5.5 35.58±5.6 34.29±3.9 41.18±11.2
NOS + Apron XL-LS 28.04±7.2 36.67±5.9 32.15±2.4 36.56±7.4
Control 51.87±12.5 50.55±11.3 55.57±11.3 60.59±8.2
Table 2: Effects of variety and fungicide on number of panicles, panicle weight and grain
yield of pearl millet in Maiduguri during the 2006 and 2007 growing seasons
Fungicide
Neem oil spray 68.67±12.3 74.90±12.4 1933±187.7 2325±213.2 1011±102.4 1179±106.7
(NOS)
Apron XL-LS 96.78±10.5 86.67±11.4 3075±219.1 2600±165.3 1708±121.5 1472±107.3
NOS + Apron XL- 99.56±9.8 93.11±16.8 3189±176.0 2700±142.4 1679±89.5 1737±98.9
LS
Control 54.78±7.6 66.00±9.6 1322±113.4 1778±109.0 805±43.7 973±21.5
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Aliyu, B et al: Continental J. Agronomy 2: 1 - 7, 2008
Table 3: Interaction of variety and fungicide on grain yield of pearl millet in Maiduguri during the 2007
growing season
Number of panicles
Variety Neem oil spray Apron XL-LS NOS + Apron XL- Control
(NOS) LS
SOSAT-C88 1683±121.4 2006±154.7 2567±176.3 1150±88.2
Ex-Borno 1130±56.3 1295±113.4 1427±132.2 1070±66.2
GB8735 723±12.5 1113±102.1 1217±79.9 700±10.3
The production of panicle and grain yield was significantly influenced by the application of fungicide
(Table 2). Plants treated with metalaxyl and metalaxyl + neem oil spray showed increased panicle size and
grain yield than those received neem oil spray and the check.
The interaction effects of variety and fungicide on grain yield was significant (Table 3). The variety
(SOSAT-C88) that produced the highest yield did so when treated with the combination of metalaxyl and
neem oil spray. A general increase in yield of the other varieties was also observed. The most susceptible
variety to downy mildew was GB8735.
DISCUSSION
The fact that three varieties of pearl millet showed varied responses to downy mildew attack in the area of
study confirmed the influence of genotype difference, which is a factor in choosing germplasms for
cultivation. The variety SOSAT-C88 showed comparatively higher resistance to downy mildew than EX-
Borno and GB8735. Varietals differences in pearl millet have also been reported (Rathi and Panwar, 1997;
Singh et al., 1993).This study revealed that infection was higher at the basal tillers than on the main stems.
This could be due to the proximity of tillers to soil where if contaminated may be a readily source of
infection.
Metalaxyl is still an effective fungicide against downy mildew in pearl millet from this study and the report
of Williams and Singh (1981). However, supplementing metalaxyl with neem oil spray would generally
increase grain yield.
In pearl millet growing areas where there is infestation of downy mildew the variety SOSAT-C88 should
be used with the application of metalaxyl + neem oil spray for a better return on cost of production. There
is still the need to develop highly resistant varieties against downy mildew so that the extra cost of
fungicide would be reduced if not eliminated.
REFERENCES
Anaso, A.B. (1989): Variety selection and planting strategies of maize for control of sorghum downy
mildew in Nigeria Guinea Savanna Applied Agricultural Research 3: 196-200.
Anaso, A. B, Emechebe A. M., Tyagi, P. D. and Manzo, S. K. (1989): Assessment in yield due to sorghum
downy mildew of maize in Nigeria Guinea Savanna Tropical Pest Management 301-303.
Conrick, J. and H. Schmutterer (2004): The Neem Tree : The Ultimate Herb. The Neem Foundation web
site – www.neemfoundation.org (access date: 23/11/2007)
Food Agricultural Organization (FOA) (1985): Production Year Book. Rome, Italy 2-15.
James, W. C. (1983): Crop loss assessment pages 130-143 in plant pathologist pocket book 2nd edition ( A.
Johnston and C. Booth Eds) Commonwealth Mycological Institute Kew.
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Aliyu, B et al: Continental J. Agronomy 2: 1 - 7, 2008
Kaura, S. K., S. K. Gupta and J. B. Chowdhury (1998 ): Morphological and oil content variation in seeds
of Azadirachta indica A. Juss. (Neem) from northern and western provenances of India. Plant Foods for
Human Nutrition. 52 (4): 293-298
Kimmel, E. C., J. E. Casida and L. O. Ruzo (1986): Formamidine Insecticides and Chloroacetanilide
Herbicides: Disubstituted Anilines and Nitrobenzenes as Mammalian Metabolites and Bacterial Mutagents,
J. Agri Food Chem 34:157-161.
King, S. B. and Webster, O. J. (1970): Downy mildew of sorghum in Nigeria India Phytopathology
4: 212-319.
National Research Council (NRC) (1996): Lost crops of Africa vol. 1 Grains National Academy Press
Washington D.C. 36: 1-12.
Nene, Y. L. and Singh, S. D. (1976): Downy mildew and ergot of pearl millet, pest Africa and News
Summaries Darker 22 (3): 366-385.
Nwasike, C. C., E. F. I. Baker and P. N. Egharevba (1983): The Potential for Improving Millet (Pennisetum
typhoides) in farming systems of the Semi Arid Areas of Nigeria. Agriculture and Environment 7: 15-21.
Rai, K. N. and Kumar, K. A. (1994): Pearl millet improvement at ICRISAT an Update International
sorghum and millets Newsletter 35: 1-29.
Rathi, A. S. and Panwar, M. S. (1997): Downy Mildew reactions of pearl millet varieties and
parents.International Sorghum and Millets News Letter No. 38, 128-130.
Schmutterer, H. (2002): The Neem Tree: Source of Unique Natural Products for Integrated Pest
Management, Medicine, Industry And Other Purposes (Hardcover),2nd Edition, Weinheim,Germany: VCH
Verlagsgesellschaft .ISBN 3-527-30054-6 P. 23-42
Singh, S. D., Balls, S. L. and Thakur, D. P. (1987): Problems and strategies in the control of downy
mildew ( a review article). Proceedings of the International Pearl millet Workshop International Crops
Research Institute for the Semi-Arid Tropics, Patancheru AP (India) 161-172.
Singh, S. D., King, S. B. and Werder, J. (1993): Downy mildew disease of pearl millet. International
Bulletin International crops Research Institute for the Semi-Arid Tropics (India) 37: 25-35.
Thakur, R.P. and King, S. B. (1988). Downy mildew disease of pearl millet, Information Bulletin No.2
International Crops Research Institute of Semi-Arid Tropics India 5-17.
Waller, J. M. and Ball, S. L. (1983): Interaction Between pearl millet varieties and Sclerospora
graminicola. Plenum Press London. 5-20.
Williams, R. J. (1984): Downy mildew of tropical cereals. Advance in Plant Pathology 2: 1-03.
Williams, R. J. and Singh S. D. (1981): Control of pearl millet downy mildew by seed treatment with
metalaxyl. Annals of Applied Biology 97: 263-268.
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Aliyu, B et al: Continental J. Agronomy 2: 1 - 7, 2008
Corresponding Author:
Aliyu, B.
Department of Biological Sciences, Gombe State University, P.M.B. 127, Gombe, Nigeria
aliyubabale@yahoo.com
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Continental J. Agronomy 2: 8 - 18, 2008
©Wilolud Online Journals, 2008.
ABSTRACT
An investigation was carried out in Akure, Nigeria on the effect of sole and fortified
agro-industrial biomass for improving soil bulk density and porosity improvement,
root growth and pod yield of okra (Abelmoschus esculentum L)
The 20 organic fertilizer treatments were compared to chemical fertilizers (NPK 15-
15-15 fertilizer ) and a control (no fertilizer; no manure), replicated four times and
arranged in a randomized complete block design.
The results showed that the application of 6/ha of agro-industrial biomass in sole
forms or fortified with goat, pig and poultry manure increased significantly (P<0.05)
the soil porosity, root growth and pod yield of okra compared to the control treatment.
The soil bulk density was also reduced significantly compared to the control
treatment.
The amended woodash, spentgrain, cocoa husk, ricebran and sawdust with pig, goat
and poultry manure reduced the soil bulk density values more than their ordinary
forms. The NPK fertilizer and the control plots had relatively high bulk density and
low porosity values.
Spentgrain applied sole or amended with manure most reduced the soil bulk density
followed by woodash, cocoa husk, ricebran and sawdust respectively. This explained
why the spentgrain (sole and amended forms) treatments had the best soil porosity,
root growth and pod yield of okra compared to others.
The linear correlation “r” values between root length and pod yield of2 okra were
0.40, 0.45, 0.57 and 0.68 at 1% level for crop 1, 2, 3, and 4 respectively showing that
it is an important factor in the determination of okra pod weight.
Spentgrain applied sole or amended with animal manure was very useful in
improving soil physical properties of a lowly fertile humid soils for sustainable
agriculture.
KEYWORDS: Sole and amended agro industrial biomass, bulk density, porosity,
root growth,pod yield and okra
INTRODUCTION
The physical resistance of the soil to the growth of crop seedlings and yield at different stages is an
important factor in crop production particularly getting the right population density and development to
maturity.
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E.I. Moyin – Jesu : Continental J. Agronomy 2: 8 - 18, 2008
Aina et al (1985) reported that soil parameters known to affect the mechanical impedance included soil
texture, bulk density and moisture content. The effect of soil compaction which commonly results from
cultural practices such as continuous land clearing and tillage practices expose soils to deterioration of its
organic matter and loss of essential nutrients for crop growth. Impedance to crop seedlings growth
increases with soil bulk density.
Aina (1979) reported that as a result of diminished soil organic matter during a ten year continuous
cultivation of an Iwo soil S. W. Nigeria, there was a considerable reduction in soil aggregation, porosity,
hydraulic conductivity and increased bulk density. The ultimate consequence was decline in soil fertility.
Obi and Ofoduru (1997) reported that the use of mineral fertilizers such as NPK, Urea and Ammonium
sulphate led to degradation of physical qualities of soils caused by low organic matter levels. This was
supported by Zake (1973) who stated that a single heavy dose of soluble fertilizers might not work in the
low activity clay soils, and they required an organic matter to impart appropriate chemical, physical and
biological properties.
Although, the effects of bulk density and soil moisture regime on soil compaction and crop growth have
been reported for some Nigerian soils (Ultisol and Oxisols) especially from the approach of tillage practice.
(Ojeniyi, 1985 and, Lal, and Maurya, 1979). However, there is scarcity of research information on the use
of agro-industrial biomass such as cocoa hush, woodash, sawdust, ricebran and spentgrain (brewery
waste) applied in sole or amended with goat, pig and poultry manures for reducing soil bulk density,
increasing porosity, root development and yield of okra.
Therefore, the objectives are to determine the influence of agro-industrial wastes (Sole and amended forms)
for soil bulk density and porosity improvement, root development and pod yield of okra (Abelmoschus
esculentum Moench L).
The % porosity was calculated from the values of bulk density. The mechanical analysis of the soil was
done by the hydrometer method. Bouycous (1951).
The organic materials were processed to allow decomposition. The dried cocoa pod husk was ground using
hammer mill, woodash was sieved to remove burnt charcoal and pebbles. The spentgrain, ricebran and
cocoa husk were each partially composted separately. The pig, goat and poultry manures were stacked
individually to allow quick mineralisation processes.
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The processed forms of the organic materials were analysed. The nutrient contents in the organic materials
were determined using wet digestion method based on 25-5-5ml of HNO3-H2 S O4-HCIO4 acids. The
filtrates collected, were evaluated for the C, N, P, K, Ca, Mg and Na contents and micronutrients (AOAC,
1970).
Field Experiment
The land was cleared, ploughed and harrowed. The soil was under arable crops for 10 years. The four field
experiments were conducted on the early and late crops in 1998 and 1999 at the same site and each
experiment spanned for four months.
Twenty organic fertilizer treatments, sole or amended were applied to each crop of okra, a reference
treatment NPK 15-15-15 fertilizer (400kg/ha) and the control treatment (no manure, no fertilizer).
The five agro-industrial biomass were woodash, ground cocoa husk, ricebran, spentgrain (sorghum based
brewery waste) and sawdust. The materials were applied sole at 6tha and each plant residues was
combined with goat, pig and poultry manure at the rate of 3tha each.
The 22 treatments were replicated four times on each of the four consecutive okra crops and arranged in a
randomized complete block design while the size of each of the plots was 4mx4m (16m2)
The sole and amended residues were incorporated into the soil ten days before planting okra seeds using
garden fork to allow easy decomposition. Four seeds of early maturing okra variety (NHAe-47-4) were
planted per hole of 2cm deep at a spacing of 60x30cm. Germination took place five days planting and later
thinned to one plant per stand.
The plots were manually weeded thrice starting from the second, fifth and seventh weeks after planting.
The insect pests were controlled by spraying vetox 85 at the rate of 28g a.i in 9Lt of water starting from
second week after planting (WAP).
Harvest of the mature pods started at 40 days after planting and it continued at every four days interval until
senenscence The total weight of harvested pods were determined (kg/ha) and at the end of each
experiment, all the okra plants were uprooted and seminal root lengths were determined.
The high soil bulk density values affected the soil porosity, root growth and yield of okra in the control
treatment as well as contributing to low organic matter status of the soils. This finding agreed with that of
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Ojeniyi, 1985 who reported that there was a strong relationship between soil bulk density and organic
matter status in a soil.
Ricebran and sawdust have relatively high C:N compared with cocoa husk, woodash and spentgrain,
therefore, they are expected to be less efficient in returning plant nutrients and organic matter for the
reduction in soil bulk density, improved root growth and yield of okra.
Effect of agro-industrial biomass on the soil bulk density, porosity, root growth and yield of okra.
The data on the soil bulk density values (Table 3), soil porosity (Table 4), root growth (Table 5) and pod
weight of okra (Table 6) under the different agro-industrial biomass were presented. The sole and amended
agro-industrial biomass increased significantly (P<0.05) the porosity, root growth and pod weight of okra
compared to the control treatments for the four corps of okra.
Soil bulk density was reduced with each addition of manure from the first to fourth crop of okra.
Amendment of woodash, spentgrain, cocoa husk, ricebran and sawdust with animal manure reduced the
soil bulk density values more than their ordinary forms. The NPK fertilizer and the control plots had
relatively high bulk density values. Spentgrain applied sole or amended with manure most reduced the soil
bulk density compared with woodash, cocoa husk, ricebran and sawdust.
Although, the spentgrain had the least C,N,P,Ca,Mg, Fe, Mn, Cu and Zn status compared to cocoa husk
and woodash, it most reduced the bulk density out of the five agro-industrial biomass through its relatively
low density and probable relatively high porosity. The combination of its better fertility status and best
porosity would have enhanced root growth and yield of okra.
The reduction in soil bulk density by application of spentgrain should have positively influenced other soil
physical properties such as aeration and water infiltration, thereby enhancing root growth, hence, it was
discovered that the spentgrain most improved the growth of okra compared to others.
This finding is significant considering the fact that soil bulk density is an important soil parameter in crop
production. Aina (1979) reported that as a result of diminished soil organic matter during a ten years
continuous cultivation of an Iwo soil in S.W. Nigeria, there was a considerable increase in soil bulk density
and serious reduction in soil aggregation, porosity and hydraulic conductivity. The ultimate consequence
was decline in soil fertility, hence, low root growth, high bulk density and low yield of okra under control
treatment.
Obi and Ofoduru (1997) also reported that the continuous use of NPK fertilizer in soil would lead to
degradation of physical properties of soils especially bulk density caused by low organic matter levels and
this could be responsible for the increased values of soil bulk density in NPK fertilized soils compared to
the sole and amended agro-industrial biomass.
The best yield of okra associated with the use of spentgrain is also attributable to its mostly reduced soil
bulk density out of all the agro-industrial biomass. This is corroborated by the fact that okra grown on
spentgrain manured soil had the longest roots and the enhancement of soil structural condition might have
improved root growth, nutrient and water uptake. The improvement of soil physical condition by applied
spentgrain is consistent with the assertion of Woomer and Muchena (1993) that continuous productivity of
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tropical soils is associated with maintenance and improvement of soil physical characteristics which can be
further improved by the use of organic fertilizers.
It is, therefore, recommended that sole and fortified agro-industrial biomass such as woodash, spentgrain
and cocoa husk applied at 6t/ha are very useful organic fertilizers for improving soil porosity, root
development ad pod weight of okra and reduced soil bulk density. The use of sole and amended spentgrain
was particularly the best in improving soil porosity, root growth and pod yield of okra and reduced soil
bulk density.
This recommendation is very important because continuous use of inorganic fertilizers for soil fertility
improvement is accompanied by destruction of soil physical properties, besides, such fertilizers are very
expensive and scarce beyond the scope of poor resources farmers who are still the major producers of food
in tropical countries of Africa, Asia and so forth.
REFERENCES
Aina, P. O. (1979): Soil changes resulting from long term management practices in Western Nigeria, Soil
Sci. Soc. Amer. J. 43: 173-177.
Aina, P. O., Fapohunda H. O. and Idowu J. (1985): Compaction and moisture effects on soil strength and
crop emergence. Ife Journal of Agric. Vol. 7Nos 1 & 2 : 16-25.
A.O.A.C., (1970): Official method of analysis 12th edition. Association of official Analytical Chemist
Washington, D. C. U. S. A.
Bouycous, H. (1951): Mechanical analysis of soils using hydrometer method. Anal. chem. Acta 22: 32 -
34.
Lal, R and Marya, P.R. (1979): Effects of bulk density and moisture on radicle elongation of some tropical
crops, pp 337-347. In R. Lal and D. J. Greenland (eds). Soil physical properties and crop production in the
tropics John Wiley and Sons 551pp.
Obi, M. E. and Ofoduru, C. O. (1997): The effects of soil amendments on soil physical properties of a
severely degraded sandy loam soil in south-eastern Nigeria. 23rd Annual Conf. Soil Sci. Soc. Of Nigeria
held in Usman Danfodio University, Sokoto March 2-7, 1997 pp 6-9.
Ojeniyi, S. O. (1985): Effect of replacement grass and forest with tree crops on Nigeria soil textural and
chemical properties. A paper presented at International Conference on soil fertility, soil tilth and post
clearing land degradation in the humid tropics held at University of Ibadan, Ibadan Nigeria July 21-26,
1985.
Woomer P. L. and Muchens, F. N. (1993): Overcoming soil constraints in crop production in tropical
Africa. Sustaining soil productivity in intensive Africa Agriculture. Wageningen. CTA, 1993, seminar
proceedings Accra (Ghana). November 10-17, 1993 pp45.
12
E.I. Moyin – Jesu : Continental J. Agronomy 2: 8 - 18, 2008
Zake, J. Y. L. (1993): Overcoming soil constraints of crop production. In sustaining soil productivity in
intensive African agriculture, Wageningen CTA, 1993, seminar proceedings, Accra, Ghana, Nov. 15 -19,
1993 pp 57-61.
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E.I. Moyin – Jesu : Continental J. Agronomy 2: 8 - 18, 2008
Table 2 : Chemical Composition of the organic materials used for the experiment
Treatments C N C:N P Ca K Mg Fe Mn Cu Zn
Cocoa husk 16.00 1.44 11.11 100 9.34 20.60 7.10 50.40 8.40 0.55 1.77
Woodash 18.00 1.53 11.80 86.00 9.40 23.00 8.50 65.50 11.90 0.66 1.88
Spentgrain 100.00 0.78 12.80 56.00 0.13 7.90 3.10 3.40 0.99 0.10 0.70
Ricebran + 14.00 0.60 23.30 76.00 0.12 7.90 1.80 6.30 1.80 0.18 0.50
Sawdust + 8.00 0.42 19.00 110.00 0.10 5.10 1.30 4.00 1.70 0.16 0.40
Poultry manure 30.00 4.33 6.93 385.00 3.20 9.72 4.10 37.85 1.66 0.15 1.26
Goat manure 20.00 2.52 7.93 167.50 2.90 9.97 4.50 34.50 1.60 0.16 1.30
Pig manure 25.00 3.72 6.72 312.00 3.10 14.45 4.80 34.00 1.62 0.17 1.34
+ Undergo decomposition for six weeks before analysis.
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E.I. Moyin – Jesu : Continental J. Agronomy 2: 8 - 18, 2008
TABLE 3: The effect of plant residues plus manure on soil bulk density (g/cm3) under the four crop of
Okra
S/N TREATMENTS 1ST 2ND 3RD 4TH 5TH
12. Rice Bran + Goat Dung 1.54ij 1.51ij 1.49mn 1.45lm 1.50hi
13. Rice Bran + pig Dung 1.53hi 1.51ij 1.48lm 1.42j 1.49gh
16. Spent Grain + Goat Dung 1.46b 1.42b 1.36c 1.29bc 1.38h
17. Spent Grain + pig Dung 1.47bc 1.43bc 1.32a 1.28b 1.38b
20. Saw Dust + Goat Dung 1.53hi 1.52jk 1.49mn 1.43jk 1.49gh
21. Saw Dust + pig Dung 1.53gh 1.51ij 1.47kl 1.44kl 1.49gh
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E.I. Moyin – Jesu : Continental J. Agronomy 2: 8 - 18, 2008
Table 4: The effect of agro-industrial biomass on % soil porosity under the four crops of Okra.
Treatment means within each group or column followed by the same letters are not significantly
different from each other using DMRT at 5% level.
16
E.I. Moyin – Jesu : Continental J. Agronomy 2: 8 - 18, 2008
Table 5: The effects of plant residues plus manure on the root length (cm) under four crops of
Okra.
12. Rice Bran + Goat Dung 13.74d 20.546d 26.436d 26.148d 21.72e
13. Rice Bran + Pig Dung 16.28f 25.085hi 32.288i 32.648g 26.78j
16. Spent Grain + Goat Dung 20.248j 30.42k 38.87n 39.562k 32.28q
17. Spent Grain + Pig Dung 19.56h 29.354j 36.092m 38.558j 30.89no
20. Saw Dust + Goat Dung 16.50f 24.426g 31.474h 31.79f 26.05hi
21. Saw Dust + Pig Dung 17.22g 27.18l 34.298k 33.694h 28.10kl
22. Saw Dust + Poultry manure 18.28h 27.33i 35.492lm 35.548l 29.16m
Treatment means within each group of column followed by the same letters are not significantly different
from each other using DMRT at 5% level
17
E.I. Moyin – Jesu : Continental J. Agronomy 2: 8 - 18, 2008
Table 6: The effect of plant residues plus manure on fresh pod weight of Okra (gross plot) under four crops
of Okra in kg/hectare.
Treatment means within each group or column followed by the same letters are not significantly different
from each other using DMRT at 5% level.
18
Continental J. Agronomy 2: 19 - 24, 2008
©Wilolud Online Journals, 2008.
ABSTRACT
Fertility status of soils supporting Raphia hookeri at Onuebum in Bayelsa State,
Nigeria, was investigated. The soils have silt ⁄ (clay + silt) ratios very low, being less
than unity in all the samples which is an indication that most of the silt has been
weathered into clay. The soils pH were moderately to strongly acidic with their
values ranging from 4.50 - 4.52. The hydromorphic soils supporting the growth of
Raphia hookeri palms contained low concentration of total N, available P and
relatively low exchangeable cations, Ca, Mg, K and Na. The base saturation percent
is medium to high (66.0 – 81.0%) and low in effective cation exchange capacity
(4.754 – 6.416 cmol ⁄ kg –1). The plantation therefore require application of organic or
inorganic fertilizer preferably N and K to boost the soil nutrient. In addition, good
tillage and liming should be done to reduce the flooding and acidity of the soils.
INTRODUCTION
Raphia palm is monocotyledonous tree crop that thrives on predominantly swampy areas which are mostly
hydromorphic soils. It is a utility plant that supplies numerous products of social and economic
importance especially in Southern Nigeria. The palms valued for their fibre (piassava), furniture materials,
and cosmetic by products, and palm sap called palm wine, which is rich in vitamins, carbohydrates and
yeast (Obahiagbon, 2007). Major constraints to crop production in tropical Africa generally include biotic
and abiotic factors. Among the biotic factors is the soil. Soil is the major contributor to crop yield, this is
because conducive soil factors supply enough plant nutrients which are essential for the growth,
development and yield of crops. Thus soil testing is essential to the determination of the potentials and
constraint of soils to crop.
In order to fully exploit raphia palm, there is need to know the soil properties of the hydromorphic soil on
which the palm grows and how it can be properly managed for optimal growths, development and yield.
Hydromorphic soils are characterized by an excess of soil water, at least for a short period of time. They
are generally coarse textured with high acidity except in few patches with calcareous relies. Organic matter
and nitrogen contents vary from low to moderately high depending on the intensity of water level and
duration of water logging which influence the rate of organic matter degradation. The soils are generally
low in exchangeable bases particularly potassium and magnesium.
Raphia palms farmers will encounter difficulties in managing low fertility soils, especially where there are
no baseline data on inherent physical and chemical properties of the soils and the limits of nutrient
requirements of raphia palm. Increased production will not only require additional inputs of N, P, K and
Mg but also micronutrients to sustain high yields. At present there is little information on the fertilizer
requirements for raphia palm in Nigeria. The available information is based on extrapolations from nursery
fertility trials. Attempts at developing effective management of the soils supporting raphia palm, requires
the use soil test data because it is essential to the determination of the potentials and constraints of soil to
crop yield.
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Imogie, A.E et al: Continental J. Agronomy 2: 19 - 24, 2008
Since little seems to have been directed to identifying fundamental constraints of soils grown to Raphia
hookeri in Onuebum in Bayelsa State. This present study aims at evaluating the soil properties of the area,
identify constraints militating against the growth, development and yield of Raphia hookeri and to suggest
appropriate correction measures to increase growth, development and yield of the palm.
Table 1: Particle size distribution and textural classes of hydromorphic soils supporting Raphia hookeri
at Onuebum NIFOR Sub-station.
Table 2: showed the chemical properties of soils supporting Raphia hookeri. The result of the chemical
analysis showed that the soils were moderately to strongly acidic, having a pH value ranging from 4.30 to
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Imogie, A.E et al: Continental J. Agronomy 2: 19 - 24, 2008
4.71 with a mean of 4.50 for surface and 4.26 – 4.77 with a mean of 4.52 for sub-surfaces soil. This is
rated as strongly acid (Holland et al., 1989). According to Jacquemard et al., (1998), oil palm can cope
with acid soils with a pH as low as 4 or 45.
Raphia palm like oil palm can tolerate acid soils. The organic carbon contents of the soils were low
ranging from 0.22 – 1.256gkg-1 with a mean value of 0.650gkg-1 for surface soils and 0.032 – 0.960 gkg-1
with a mean value of 0.502gkg-1 for sub-surface soils. The soil total N was generally low in the three
locations. These ranging from 0.067 – 0.245 with a mean value of 0.096 gkg-1 for surface soils and 0.025 –
0.522 gkg-1 with a mean value of 0.076 gkg-1 for sub surfaces soils. The low total N values could be
attributed to ineffective microbial degradation of organic matter in the waterlogged soils and also due to
long period of flooding which usually last for 6 – 8 months in a year with few months of dryness.
There is a high variation in the degree of decomposition of the soils organic matter. And this is a reflection
of the organic carbon content in the soils (Onyekwere et al., 2003). Raphia palm are heavy feeder of N
thus low content of N will likely have adverse effect on growth, development and yield. Thus external
application of N in form of organic or inorganic fertilizer is required to enhance the growth, development
and yield of the palms.
The available P was generally low except in location two where the values were moderately high. The
mean values at this location ranged from 17.80 mgkg-1 for surface soils to 13.85 mgkg-1 for sub surface
soils. Locations 1 and 2 have available P values less than 10 gkg-1 suitable for optimal productivity (FAO,
1976). Raphia hookeri is not a heavy feeder of phosphorus; hence the P content in these soils is not likely
to limit the growth, development and yield of the palm. The exchangeable calcium, magnesium, potassium
and sodium were very low.
The calcium contents were very low and less than 4.0 cmol kg-1. According to Bohn et al., (1979), high
calcium content is very good for crop production, because it is an indication of low concentration of other
potentially troublesome exchangeable cations like sodium and aluminum in acid soils. Exchangeable K
and Mg were low. The lower contents of these basic cations may be caused by a high degree of leaching
which is certainly aggravated by the high rainfall, sandy texture and the low pH of the soils.
Exchangeable sodium content was moderate. The value ranged from 0.611 – 1.522 cmol kg-1 with a mean
value of 0.622 cmol kg-1 for surface soils to 0.585 – 1.522 cmol kg-1 with a mean value of 0.768 cmol kg-1
for sub-surface soil. The exchangeable acidity ranged from 1.05 – 2.04 cmol with a mean value of 1.55
cmol kg-1 for surface soils and 1.05 – 1.56 cmol kg-1 with a mean value 125 cmol kg-1 for sub-surface soils.
The highest value was recorded at location 1 with the least value recorded at location 3. The effective
cation exchange capacity (ECEC) was generally low, the values ranges from 3.331 – 8.012 cmol kg-1 with
mean value of 4.757 cmol kg-1 for surface soils and 3.284 – 10.515 cmol kg-1 with a mean value of 6.416
cmol kg-1 for sub-surface soils.
According to FAO (1976), soils having ECEC value above 20 cmol kg-1 are regarded as being suitable for
crop production, if other factors are favourable. The percentage base saturation values were moderate to
high with all the values above 35% which is regarded as the critical level for the growth, development and
yield of palm (Ibanga and Udo, 1996).
However, because water logging brings about increase in pH with attendant decrease in exchange acidity;
the soils generally have high percentage base proportion of the reserves in exchangeable or extractable
available forms. This could be the reason for high percent base saturation as observed. Thus these soils
could support any plant that tolerates permanent or periodic wetness like Raphia hookeri palms. Generally
it was observed that the soil at Onuebum where Raphia hookeri is cultivated had low CEC and this is an
indication of a low buffering capacity. Also the soil were inherently low in total N, available P, thus for
21
Imogie, A.E et al: Continental J. Agronomy 2: 19 - 24, 2008
optimal productivity of the palms, external inputs such as application of NPK and Mg fertilizers in form of
organic or inorganic fertilizer is required to sustain growth and productivity.
(2) Drainage
Onuebum soils usually is flooded 6-8 months in a year, thus for effective utilization of this land for
meaningful productivity, excess water should be removed through good drainage system or channels. The
objective of soil drainage is to control excess water so as to make the soil more stable for optimum plant
growth. The excess water on the surface of the soil can be drained either by open drains or sub-surface
methods such as the use of tile; mole drains perforated pipes as well as swap and pump approaches. Apart
from leaching excess salts from the soil, drainage ventilates the soil, moderates soil temperature and makes
soil moisture available to the rooting cane. Other benefit is that drainage elongate crop growing season and
makes early planning and planting easy.
ACKNOWLEDGEMENT
The authors are grateful to the Executive Director of the Nigerian Institute for Oil Palm Research (NIFOR)
for his support in kind and cash for the success of this work. Important to thank too is the plantation
manager at NIFOR Raphia palm substation, Onuebum, Mr. Adejaro, and the entire staff of the station for
their high sense of duties, commitment and dedication for the successful collection of soil samples.
REFERENCES
Aduayi, E.A. and Ekong E.E. (1981), General Agriculture and soils. Cassel Limited, an Affiliation of
Macmillan Pub. Co. Inc, 8th edition, New York: 55.
Bohn, H.L., Brian, L.M. and George, A.O. (1979). Soil Chemistry. John Wiley and Sons. New York,
329pp.
Bouyoucos, G.H. (1951). A re-calibration of the hydrometer for mechanical analysis of soils 8th edition,
Macmillan New York. 32pp.
Bray R.H. and Kurtz, L.T. (1965). Determination of total and available forms of phosphorus in Soils.
Journal of Soils Science, 53:39-45.
22
Imogie, A.E et al: Continental J. Agronomy 2: 19 - 24, 2008
Bremmer, J.M. (1965). Total Nitrogen in C.A. Black (ed). Methods of Soil Analysis, Part 2,
Agronomy Series 9 American Society of Agronomy, Madison, Wisconsin USA, 1149-1170.
Holland, M.D., Allen, R.K.G and Murphy, S.T. (1989) Land evaluation and agricultural
recommendations, Cross River State National Park, Oban Division, Bulletin No 18.
Ibanga, I.J. and Udo, E.J. (1996). Soil Survey and fertility baseline data collection of Akwa Ikot Effanga
Farm, Agricultur al Development Project, Bulletin No. 2
Jackson, M.L. (1968). Soil Chemical analysis, I.I. T.A. 43: 35-38.
Jacqueward J.J. C., Baudouin, L., Berthaud, A., Graille, J., Huguest, R., Marriou, D.I., Noel,
J.M., Quencez, P and Tailliez, B. (1998). Oil palm (The Tropical Agriculturist). Macmillan,. Basingstoke
London. 647Pp.
Mclean, E.O. (1965). Aluminium. In. C.a. Black (ed), Methods of Soil Analysis. Agronomy
Number 9 part 11. American Society of Agronomy, Madison, Wisconsin, 978 – 999.
Obahiagbon, F.I. (2007). Raphia palm sap production uses. A paper presented at NIFOR
Seminar held 19th February, 2007, Pp 3.
Onyekwere, I.N., Ano, A.O., Ezenwa, M.I.S., Osunde, A.O. and Bala, A. (2003). Assessment of
exchangeable acidity status and management of wetland soils of Cross River, Nigeria. In Ojeniyi et al.,
(eds). Agricultural productivity and Rural poverty. Environmental implication pp. 202 – 207.
Stewart, V.I., Adams, W.A. and Abdulla, H. H. (1970). Quantititative pedological studies on soils derived
from Silurian mudstone I. parent material and the significance of weathering process. Journal of soil
science 21: 242-247.
Walkley, A and Black, I.A. (1934). An examination of the degtjareff method for determing soil organic
matter and a proposed modification of the chromic acid titration
methods. Soil Science 37: 29-28.
Corresponding Author:
Imogie, A.E.
Agronomy Division, NIFOR, PMB 1030, Benin City, Edo State
Email: imogie@yahoo.com
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Imogie, A.E et al: Continental J. Agronomy 2: 19 - 24, 2008
24
Continental J. Agronomy 2: 25 - 30, 2008
©Wilolud Online Journals, 2008.
INTEGRATION OF COCOA POD ASH, POULTRY MANURE AND NPK 20:10:10 FOR SOIL FERTILITY
MANAGEMENT – INCUBATION STUDY
Ayeni L.S.
University of Agriculture, Department of Soil Science and Land Management, Abeokuta, Nigeria. E- Mail.
leye_sam@yahoo.com
ABSTRACT
A laboratory incubation study to determine the rate of nutrient supply by poultry manure (0, 5
and 10 t ha-1), cocoa pod ash (0, 5 and 10 t ha-1), NPK 20:10:10 fertilizer (0 and 150 kg ha-1)
and their combinations on soil properties was conducted at Igunshin in the rainforest zone of
Southwest Nigeria. The 10 treatments were arranged in completely randomized design with
nine replications. The soil was slightly acidic, low in OM, N, P and K. The incubation study
showed that the soils treated with poultry manure (P) rates, cocoa pod ash(C) rates and their
combinations significantly (p<0.05) increased soil pH over the control and NPK 20:10:10
fertilizer (F). When poultry manure was combined with NPK 20: 10:10 fertilizer, the pH of the
soil decreased as the incubation period progressed while the pH increased with combined
cocoa pod ash and the mineral fertilizer at 90 days of incubation. The rate of decrease in soil
pH was more pronounced in the soils treated with NPK fertilizer alone. Total OC, total N,
exchangeable K and Mg significantly (p<0.05) increased as the period of incubation increased
in all the treatments compared with control. Cocoa pod ash rates and cocoa pod ash combined
with NPK fertilizer increased exchangeable Ca as the period of incubation increased while
poultry manure rates and its combinations with NPK fertilizer decreased at 90 days of
incubation. Combined poultry manure and NPK fertilizer had the highest N and P while
combined cocoa pod ash recorded the highest Ca, K and Mg. Combination of poultry manure
with reduced level of mineral fertilizer or cocoa pod ash had positive effect on the acid soil and
increased the rate of nutrient mineralization than the sole application of poultry manure or
cocoa pod ash.
KEY WORDS: Cocoa pod ash, poultry manure, integration, mineral fertilizer
INTRODUCTION
Recently research effort has shifted from single application of organic manures and mineral fertilizers to integrated
plant nutrition management around the efficient use of low level of mineral fertilizer with organic manures in order
to reduce the problems associated with sole use of mineral fertilizers and organic manures. In Nigeria, scarcity of
mineral fertilizers, inefficient distribution channel and unjustly application without soil tests are major obstacles to
the sole use of mineral fertilizers for optimum crop production. The acidic nature of these mineral fertilizers also
poses threat to the tropical soils which are known to be acidic, fragile, kaolinitic and low in nutrient status. Organic
manures on the other hand are bulky, low in nutrient quality, pose environmental health hazard and slow in
mineralization hinder its sole use in soil fertility improvement.
Cocoa is widely grown in southwestern Nigeria and its husk is known to be rich in plant major and minor nutrients
(Odedina, et al., 2003 Ayeni et al., 2008). It is advocated that cocoa husk be burnt to serve as farm sanitation. Also,
its direct use as source of plant nutrients may lead to the spread of Phytophtora palmivora causing black pod disease
of cocoa, thereby causing another problem for poor resource farmers. Transporting and milling large quantity of
cocoa pod will also add to the farmers’ cost of production. Burning of cocoa pod can serve as source of potash
fertilizer (Adu-Dapaah et al., 1994).
Poultry manure on the other hand, is widely used to serve as source of plant nutrients to supply N, P and
micronutrients (Akanni and Ojeniyi, 2007), but; large quantity is needed to supply adequate of these nutrients to
meet crop requirements. With boost in poultry companies in Nigeria, poultry litter is found in urban cities, that;
constitutes nuisance and poses health hazards. Most farmers feel reluctant to use it to fertilize their farms because of
the short falls.
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Ayeni L.S: Continental J. Agronomy 2: 25 - 30, 2008
Adeniyan and Ojeniyi (2006) have tested poultry manure combined with NPK fertilizer in experiments conducted
on soil chemical properties, maize yield and nutrient uptake by maize in southwest Nigeria. Cocoa pod ash
combined with NPK fertilizer (Ayeni et al., 2008) has also been used in soil fertility management in southwest
Nigeria. Cocoa pod is more available in Igunshin southwest Nigeria than poultry manure. Hence, its combined use
with NPK fertilizer will be more beneficial to the poor resource farmers who see cocoa pods as waste products that
need to be discarded than poultry manure combined with NPK fertilizer that is not as readily available as cocoa pod.
This experiment aimed at comparing rate of nutrients release by combined application of poultry manure and
NPK2010:10 fertilizer with combined cocoa pod ash and NPK 20:10:10 fertilizer in Igunshin southwest Nigeria .
Table 1: Properties of poultry manure and cocoa pod ash used in the experiments (%).
_____________________________________________________________________
Nutrient OC N C/N P K Ca Mg S
______________________________________________________________________
Poultry Manure 11.8 1.72 6.9 9.56 3.87 2.66 1.09 2.72
Cocoa pod ash 12.4 0.99 12.5 2.50 15.13 3.40 1.76 1.11
_______________________________________________________________________
Soil analysis
Soil samples were taken from the site at 0-20cm depth for the experiment. The collected soil samples was air-dried
and sieved through 2mm sieved mesh. Part of the soil sample was used for routine soil analysis and laboratory
incubation study. Organic matter was determined by the dichromate oxidation method. Available phosphorus was
extracted with Bray-1-P and determined colorimetrically (Bray and Kurtz, 1945). Exchangeable bases (Ca, K and
Mg) were extracted with 1N ammonium acetate at pH 7.0. Potassium was read using flame photometer while Ca and
Mg were determined on the atomic absorption spectrophotometer.
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Ayeni L.S: Continental J. Agronomy 2: 25 - 30, 2008
soil was sandy clay. The initial soil characteristics showed that the soil was deficient N and P (Sobulo and Osiname,
1981). The chemical properties of the poultry manure and cocoa pod ash used in the experiment was shown in
Table 1. Generally, poultry manure was richer in plant nutrients than cocoa pod ash except K and Ca.
Table 2: Effect of cocoa pod ash, poultry manure, NPK20:10:10 and their combination on soil pH and organic
carbon
Treatment 60 days 60 days
pH OC
Control 6.06 6.08 6.03 1.32 1.37 1.39
F150 6.00 5.98 5.96 1.36 1.36 1.38
C5 6.38 6.52 6.99 1.33 1.39 1.43
C10 7.45 7.58 7.67 1.16 1.49 1.53
P5 6.20 6.29 6.17 1.38 1.59 1.60
P10 6.39 6.68 6.66 1.36 1.60 1.77
C5F150 6.22 6.29 6.48 1.38 1.52 1.69
C10F150 7.34 7.38 7.49 1.39 1.59 1.69
P5F150 6.17 6.2 6.09 1.48 1.65 1.97
P10F150 6.19 6.43 6.22 1.55 1.79 2.1
LSD 0.18 0.2 0.23 0.07 0.09 0.09
(0.05)
Application of poultry manure rate, cocoa pod ash rate and their various combinations had an immediate effect on
pH of the soil used (Table 2). The pH of the soil samples treated with poultry manure, cocoa pod ash and their
combinations were significantly (p<0.05) higher than the control. Cocoa pod ash and poultry manure applied singly;
and cocoa pod ash combined with NPK fertilizer (C5F150 and C10F150) increased soil pH as incubation period
increased. NPK fertilizer applied singly decreased soil pH at 90 days of incubation. The decrease in pH of the soil
samples treated with NPK 20:10:10 fertilizer compare with cocoa pod ash shows its acidifying effect. This might be
as a result of its high ammonium content or acidulation of phosphate source used in formulation of the mineral
fertilizer. Chude (1999) indicated that the phosphate source in the agro nutrient NPK fertilizer was about 25%
acidulated.
The increases in pH of the soil samples treated with C5, C10, C5F150 and C10F150 show the liming effect of cocoa
pod ash. Ayeni et al., (2008a) observed similar increase in pH of soil treated with cocoa pod ash in the incubation
experiment performed to show the effect of combined cocoa pod ash and poultry manure on soil chemical properties
in Ondo southwest Nigeria. The decrease in pH of soil samples treated with poultry manure rates and its
combination with NPK fertilizer compare with cocoa pod ash rates might be as a result of organic acids produced by
poultry manure which also aided in acidifying the soil. Cocoa pod applied at 10 t ha-1 had the highest liming effect
as it recorded the highest pH (7.57) and Ca (6.70 c mol kg-1).
Table 2 also shows the effect of cocoa pod ash, poultry manure, NPK20:10:10 fertilizer and their combinations on
soil organic carbon within 90days of incubation. Decreases in OC in treatments C10 and P5F150 than the control at
30 days of incubation was observed (Table 2). Ano and Agwu, (2005), Machner and Nobel, (2002) observed similar
decrease in soil organic carbon when an acid soil was incubated with organic materials and attributed the
observation to increase microbial respiration stimulated by the added manure which might have caused temporary
27
Ayeni L.S: Continental J. Agronomy 2: 25 - 30, 2008
immobilization. The OC in all the treatments increased through out the period of the experiment. The significance
(p<0.05) increase in the OC in the poultry manure and cocoa pod ash combined with NPK fertilizer rates compared
with NPK fertilizer applied singly, indicates the ease with which mineralization takes place when the organic
materials were combined with mineral fertilizer.
Cocoa pod ash rates, poultry manure rates, cocoa pod ash combined with NPK fertilizer and poultry manure
combined with NPK fertilizer significantly increased soil total N, available P, K (except C5F150), Ca (except
P10F150) as the period of incubation increased (Table 3). This shows continuous mineralization of the nutrient
elements. F150 decreased total N, P and K (at 90 days) as the period of incubation increased. This shows that
mineralization in NPK fertilizer had ceased before 90 days. NPK fertilizer applied singly had pH of 5.98, OC 1.36%,
N 0.13%, P 6.97 mg kg-1, S 6.97 mg kg-1, exchangeable K, Ca and Mg 0.72, 0.85 and 0.45 c mol kg-1 respectively.
Cocoa pod ash rates had mean soil pH ranged 6.63 to 6.57, N 0.12 – 0.12%, P 6.76 – 7.59 mg kg-1, S 11.06 – 12.55,
K, Ca and Mg 2.01 – 2.52, 1.63 – 3.00, and 0.38 – 0.88 cmol kg-1 respectively. Poultry manure rates had soil pH
ranged between 6.22 – 6.58, OC 1.52 – 1.58, N 0.12 – 0.14%, P 7.40 – 13.96 mg kg-1 , S 13.11 – 35.98 mg kg-1, K,
Ca and Mg were respectively 1.74 – 1.89, 1.63 – 1.87 and 0.38 - 0.62. Cocoa pod ash (5 and 10 t ha-1) combined
with NPK fertilizer had the pH of 6.63 – 7.57, OC 1.38 – 1.39%, N 0.13 – 0.15%, P 7.59 – 10.19 mg kg-1, S 17.00
24.84 mg kg-1, K, Ca and Mg 2.33 - 2.55, 4.60 – 4.75 and 1.27 – 1.47 cmol kg-1. Poultry manure rates(5 and 10 t ha-
1
) combined with NPK fertilizer had soil pH of 6.15 – 6.28, N 0.13 – 0.16%, P 11.48 - 18.47 mg kg-1, S 30.80 –
42.89 mg kg-1, K, Ca and Mg 1.80 – 1.88, 1.66-2.41 and 0.47 – 1.35 cmol kg-1 respectively. Compared with the
nutrient critical level established for arable crops in southwest Nigeria, OM (3%), N 0.15%, P 8 – 10 mg kg-1, 0.20,
0.24 and 0.16 cmol kg-1for K, Ca ands Mg respectively, all the treatments supplied adequate nutrients except F150,
C5 and P5. The control experiment had mean soil pH of 6.06, OC 1.36%, total N 0.11%, P 5 mg kg-1, S 5mg kg-1, K,
Ca and Mg 0.50, 0.80 and 0.50 cmol kg-1 respectively.
Treatment P10F150 had the highest N, P and S while C10F150 had highest K. Treatment C10 had the highest Ca
value while C5F150 had highest Mg. This shows that integration of cocoa pod ash and poultry manure with NPK
fertilizer enhanced nutrient release than sole application of any of the fertilizer materials. Phosphorus was found to
be low in the soil treated with cocoa pod ash The Phosphorus might have formed complex with soil Ca because P is
known to form insoluble complex with Ca at pH above 7 (Busari et al., 2004). This means, additional phosphatic
fertilizer may be needed in order to correct P deficiency in the treatments that were low in available P i.e. C5, F150
and C5F150. The available K increased through out the period of incubation and was significantly higher than the
absolute control. NPK 20:10:10 combined with poultry manure or cocoa pod ash increased the exchangeable Ca and
Mg. The treatments with cocoa pod ash had higher increases in K, Ca and Mg. This confirms that cocoa pod ash is a
source of K and Ca as indicated in the work of Adu-Dapaah et al., (1994) and Ayeni et al., (2008b). The higher
cations released from combined cocoa pod ash than poultry manure indicates that the nutrients were released from
cocoa pod ash included in the combination.
CONCLUSION
Combination of poultry manure with reduced level of mineral fertilizer or cocoa pod ash had positive effect on the
acid soil and increased the rate of nutrient mineralization than the sole application of poultry manure or cocoa pod
ash. It also made nutrient available in the soil within short time, thereby could enhance increase in production of
crop with short life span. Poultry manure combined with NK20:10:10 fertilizer released more N, P and S than cocoa
pod ash combined with NPK20:10:10 while combined cocoa pod ash and NPK 20:10:10 fertilizer released more K
28
Ayeni L.S: Continental J. Agronomy 2: 25 - 30, 2008
Table 3: Rate of release of nutrients from cocoa pod ash, NPK fertilizer and their combinations within 90 days
_______________________________________________________________________________________________
Treatment N P K Ca Mg S
% mg kg -1 -------------------cmol kg -1 -------------------------- mg kg-1
_______________________________________________________________________________________________________
DAYS 30 60 90 30 60 90 30 60 90 30 60 90 30 60 90 30 60 90
Control 0.10 0.11 0.11 4.40 5.02 5.10 0.50 0.53 0.59 0.76 0.77 0.79 0.36 0.40 0.60 4.28 5.00 6.00
F150 0.13 0.14 0.12 9.00 5.92 6.00 0.75 0.80 0.60 0.77 0.79 1.00 0.48 0.40 0.46 19.10 18.00 11.00
P10 0.10 0.15 0.16 13.42 13.65 14.80 0.62 2.45 2.60 0.70 2.40 2.51 0.22 0.40 0.53 17.00 21.90 69.05
C10 0.10 0.13 0.14 6.82 7.94 8.00 2.20 2.27 3.10 3.62 2.10 3.70 0.23 0.20 0.22 7.78 8.67 12.55
P5 0.11 0.12 0.14 6.45 7.73 8.02 0.54 2.30 2.38 1.45 1.70 1.73 0.24 1.20 0.43 6.56 10.10 22.66
C5 0.11 0.11 0.13 6.02 7.11 7.15 1.00 2.10 2.92 3.10 2.30 2.36 0.23 1.20 1.21 4.99 7.50 11.06
P10F150 0.13 0.18 0.18 18.00 18.52 18.90 1.52 1.92 2.20 1.90 2.90 2.44 0.26 1.90 1.90 24.44 32.24 72.00
C10F150 0.14 0.15 0.15 10.05 10.10 10.42 1.90 2.20 3.55 2.00 6.20 6.22 1.09 1.40 1.32 20.00 25.00 29.46
P5F150 0.13 0.14 0.13 11.23 11.40 11.80 0.93 1.98 2.48 1.50 1.70 1.79 1.00 0.20 0.21 20.61 12.80 59.00
C5F150 0.13 0.13 0.14 7.20 7.44 7.72 1.30 3.10 2.59 2.49 5.22 6.09 1.00 1.70 1.72 10.00 20.00 21.00
LSD 0.03 0.04 0.04 4.32 4.72 4.97 1.27 0.37 1.11 1.56 0.39 1.12 1.44 2.56 0.25 10.37 2.77 27
29
Ayeni L.S: Continental J. Agronomy 2: 25 - 30, 2008
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