Você está na página 1de 13

Analisa Kualitatif dan Kuantitatif

Biomolekul

Metoda analisa kualitatif karbohidrat


Tes molisch

Ethanolishighlyflammable.
1Naphthol:Eye,skinandrespiratoryirritant.Causingliverdamage.
Conc. sulfuric acid is highly toxic.
Conc.sulfuricacidishighlytoxic.
Testsolution:2g1naphtholdissolvedin20mL EtOH 96%(MOLISCH
REAGENT)

Reaksi

thepresenceofconc.H2SO4 sugarsaredehydratedformingfurfuryl derivates.


Furfuralisderivedfromthedehydrationofpentoses andpentosans,while5
hydroxymethylfurfuralisproducedfromhexoses andhexosans (1).

Hydroxymethylfurfural isattackedby1naphthol,whosehydroxylgrouphasa
strongelectrondonatingresonanceeffectwhichincreasestheelectrondensityin
thebenzenering.1naphtholactsasanucleophile andisaddedtothepositive
polarizedcarbonofthealdehyde groupof5hydroxymethylfurfural.Afteran
intramolecular protonmigrationwateriseliminatedandaCCdoublebondis
formed(2).

Nowafurthernucleophilic additionof1naphtholmoleculetakesplace.Afteran
intramolecular protonmigrationtwoprotonsareeliminatedleadingtoapurplefurfuryl
dipheylmethane dye(3).

Identifikasi gula pereduksi

Thenamereducingisgivenbecausehemiacetals (andtoalesserextent
g
g
hemiketals)canreduceanoxidizingg agentsuchasBenedictsreagent.

DISAKARIDA

Polysaccharides,suchasamylose oramylopectin,dohavea
hemiacetal groupononeoftheirterminalends,but
group on one of their terminal ends but
practicallytheyarenonreducing substancesbecausethere
isonlyonereducinggroupforevery2,00010,000
monosaccharidic units.
units

Beberapa
p tes ggula p
pereduksi
Tes fehling
Tes Benedict
Tes Barfoed
Tes osazon

Metoda analisa kuantitatif karbohidrat

Metode kromtografi/elektroforesis
Metode kimia
Metode
d enzimatik
i
ik
Metode fisika
Immunoassay

Metoda analisa kualititatif protein

ifaproteinmoleculehasanetpositivecharge
(at low pH or acidic condition) or a net
(atlowpHoracidiccondition)oranet
negativecharge(athighpHorbasic
),
y
condition),itssolubilityinwaterisincreased.

Beberapa
p metode
Thebiuret
e b u et test.
test.Thisisoneofthemostgeneral
s s o e o t e ost ge e a
testsforproteins.Whenaproteinreactswith
copper(II)sulfate,apositivetestistheformation
ofacoppercomplexwhichhasavioletcolor.
f
l
h hh
l
l

Theninhydrin test.AminoacidswithafreeNH2
groupandproteinscontainingfreeaminogroups
d
t i
t i i f
i
reactwithninhydrin togiveapurpleblue
complex.
p

Thexanthoprotein test.Thisisacharacteristicreactionof
proteins that contain phenyl rings
proteinsthatcontainphenylrings.
Concentratednitricacidreactswiththephenylringtogive
ayellowcoloredaromaticnitrocompound.Additionof
alkali at this point will deepen the color to orange
alkaliatthispointwilldeepenthecolortoorange.