CURRICULUM VITAE

Dr. RAJESH KUMAR

C/O Dr. Hemalatha R. (Head & Deputy Director), Dept. of Microbiology & Immunology,
NIN, Hyderabad-500604 (Andhra Pradesh)
: +919253086059 Email & Chat: rajesh.mbu@gmail.com
OBJECTIVE
Looking for a permanent and challenging position in a quality research environment that would allow me to utilize my
skills for human wellness through scientific approaches.

ACADEMICS
♦ ICMR-Post Doctoral Fellow (2010-2012)
NATIONAL INSTITUTE OF NUTRITION, HYDERABAD, INDIA
♦ Doctoral Degree – Dairy Microbiology (2005-2009)
NATIONAL DAIRY RESEARXH INSTITUTE, KARNAL, INDIA
♦ Post Graduation – Microbiology (2003-2005)
BUNDELKHAND UNIVERSITY, JHANSI
♦ Graduation – Biology (1996-1999)
CCS UNIVERSITY, MERRUT

RESEARCH/WORK EXPERIENCE
1. Organisation: Dept. of Microbiology & Immunology, NIN, Hyderabad
Duration: May 01, 2010 – till now.
Designation: ICMR, POST-DOCTORAL FELLOW, working on rDNA techniques, in-vivo & in-vitro
experiments, Cell culturing, Microencapsulation, expression of site-specific engineered genes and
purification of recombinant and native proteins etc.
Area of research:
“Molecular Characterization of Reshuffled Bile Salt Hydrolase (Bsh) and Effect of Dietary
Inclusion of Bsh+ and Bsh- Indigenous Probiotic Lactobacillus plantarum strains of Human
Origin on Cholesterol Metabolism of Rats” under the supervision of Dr. Hemalatha R. (Head
& Deputy Director).
2. Organisation: Proteomics and Structural Genomics Lab, NDRI, Karnal
Duration: August 07, 2009 – April 30, 2010
Designation: RESEARCH ASSOCIATE, worked on isolation and assessment of the antibacterial and antifungal
activity of the novel bioactive peptides generated from LAB, cloning & expression of peptidases,
immobilization and purification of proteins like aminopeptidase, carboxylpeptidase, anti-microbial
peptides etc.
Area of research:
“Novel Approaches for Production of Nutraceuticals from Milk and Indian Herbs for
Potential Use in Functional Dairy Foods” under the supervision of Dr. J.K. Kaushik (Co-PI)
3. Organisation: Molecular Biology Unit, D.M Division, NDRI, Karnal
Duration: August 2005 – July 2009
Designation: Ph.D SCHOLAR, worked on screening of lactobacilli, Development of Bsh assays, cloning,
sequence analysis, expression and purification of recombinant and native Bsh protein. Carried out in-
vivo & in-vitro trials for anti-hypercholesterolemic effect of Bsh+ probiotic Lactobacillus in SD rats.
Area of research:
 “Molecular cloning, sequence analysis and expression of bile salt hydrolase (Bsh) from a
potential indigenous probiotic Lactobacillus of human origin” under the supervision of Dr.
V.K. Batish (Former Head & Er. Scientist).
TECHNICAL SKILLS
• Purification and characterization of recombinant and native proteins, bioactive proteins with Gel filtration,
Affinity, Ion-exchange, Hydrophobic interaction chromatography on FPLC system (Pharmacia & Akta
Purifier 10), Ultrafiltration, Rotophor (Isoelectric focussing), denaturing and nondenaturing PAGE.
Localization and identification of proteins by Western blotting and Zymograms.
• Cloning, characterization, sequence analysis of gene sequences and optimization of recombinant protein
expression. PCRs (Bsh/Species/Genus/Gradient/RAPD), agarose gel electrophoresis.
• Antimicrobial agar well assay, microtitre plate assay, Spot on lawn assay, ELISA or Microtitre plate reader
• Nucleotide and amino acid sequence submission to NCBI. Efficient use of BLAST, FASTA, ClustalW
implemented in Geneious Pro (4.6.4) for sequence analysis, characterization and Primer designing. Nucleic
acids isolation and purification. Optimization of different Colorimetric Assays. Anaerobes (Bacterial)
culturing in MK3 Anaerobic Workstation (Don Whitley).
• Maintenance and Experimental handling (Survival bleeding, faecal analysis, Dissection etc) of Spargue-
Dawley rats (Male) for hypocholesterolemic studies. Plasma HDL-, LDL-, VLDL-, Total Cholesterol and
TG estimation. Interpretation of data by statistical analysis.
• Bacterial microencapsulation/immobilization, maintenance, enumeration, preservation and propagation of
bacteria from diverse nature of samples. Characterization of Lactobacillus strains for various in-vitro
probiotic attributes, efficient handling of all kind of microbiological work.
RESPONSIBILITIES
• Designing new projects and Preparation of ICB (International Competitive Bidding) bid documents and
technical specifications for purchasing instruments like Fermentor, Anaerobic Chamber, Vacuum
Concentrator, Multi-plate reader, Refrigerated centrifuges, Microfluidizer, Water Purification System, Gel-
Documentation System, Incubator Shaker, HPLC, Inverted Fluorescent Microscope etc. for International
Competitive Bidding (ICB). Demonstration of laboratory to national and foreign visitors. Maintenance of
laboratory and its instruments.
Computer skills
Operating systems: Windows 7, Windows Vista, XP, 2000 and 98.
Software tools: Geneious Pro (4.6.4), DNA Star, Gencheck, Sequin, SYSTAT etc.
MS office: MS Word, MS Excel, MS PowerPoint. (2000, 2003 & 2007).
Others: Installation, maintenance/troubleshooting of softwares and Windows.
Publications:
Research Article:
♦ Kumar, R., Grover, S., Mohanty, A.K. and Batish, V.K. 2010. Molecular cloning and sequence analysis
of bile salt hydrolase (bsh) gene from L. plantarum MBUL90 strain of human origin” 24 (3), 2010 Food
Biotech journal (Taylor and Francis).
♦ Kumar, R., Grover, S. and Batish, V.K. “Hypocholesterolemic Effect of Dietary Inclusion of Two
Putative Probiotic Bsh Producing L. plantarum strains in Spargue-Dawley Rats” under review in British
Journal of Nutrition.
♦ Kumar, R., Grover, S. and Batish, V.K. Screening and Quantitative Determination of Bile Salt Hydrolase
(Bsh) Activity of Lactobacilli Strains. Communicated in Food Microbiology Journal.

Abstracts: (05)
• “Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous
probiotic Lactobacillus plantarum strain of human origin” in 5th Probiotics and Prebiotics and
New Foods held at University of Urbania, Rome (Sep 13-15, 2009). Authors: Rajesh Kumar, Sunita
Grover and V.K. Batish.
• “Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous
probiotic Lactobacillus plantarum strain of human origin” in International Conference on
 Microbial Biotechnology held at Punjab University, Chandigarh (March 03-04, 2009). Authors:
Rajesh Kumar, Sunita Grover, A.K. Mohanty and V.K. Batish.
• “Molecular cloning, sequence analysis and expression of bile salt hydrolase (Bsh) gene from potential
indigenous probiotic Lactobacillus plantarum isolates of human origin” in International Probiotic
Conference held at Slovakia, Germany (June 04-07, 2008). Authors: Rajesh Kumar, A.K. Mohanty,
Sunita Grover and V.K. Batish.
• “Molecular cloning of bile salt hydrolase gene from a probiotic L. plantarum of human origin” in 2nd
International Conference on Trends in Cell and Molecular Biology at Jawaharlal Nehru
university (JNU), Delhi (Jan 06-08, 2008). Authors: Rajesh Kumar, Sunita Grover and V.K. Batish.
• “Bile Salt Hydrolase activity of some potential indigenous probiotic lactobacilli of human origin” in
International Conference on Traditional Dairy Foods held at NDRI, Karnal (Nov 14-17, 2007).
Authors: Rajesh Kumar and V.K. Batish.
Acession nos: (20)
EU 477374; EU770692; EU784663; EU836149; EU822811; EU887663; FJ179486; FJ179487; GQ922598;
GQ922599; GQ922600; GQ922601; GQ922602; ACG59259; ACF35257; ACF05268; ACF04740;
ACA49878; ACF35782; ACG76118. Authors: Rajesh Kumar, Sunita Grover, A.K. Mohanty, V.K. Batish.
♦ Dissertation Training ( March 2005 - June 2005)
N.D.R.I KARNAL. India
Project Title: “Optimization of ultrafiltered retentate for manufacturing of CHAKKA by defined
strain starters” under the supervision of Dr. S.K. Tomar (Sr. Scientist).
♦ Summer Training ( June 2004 – July 2004)
MOHAN MEAKINS LTD, GHAZIABAD (U.P)
DABUR INDIA LTD, SAHIBABAD (U.P)
SCHOLARLY ACTIVITY
♦ Posters Presentation
- “Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous
probiotic Lactobacillus plantarum strain of human origin” in International Conference on Microbial
Biotechnology held at Punjab University, Chandigarh (March 03-04, 2009). Authors: Rajesh Kumar,
Sunita Grover, A.K. Mohanty and V.K. Batish.
- “Molecular cloning of bile salt hydrolase gene from a probiotic L. plantarum of human origin” in 2nd
International Conference on Trends in Cell and Molecular Biology at Jawaharlal Nehru university
(JNU), Delhi (Jan 06-08, 2008). Authors: Rajesh Kumar, Sunita Grover and V.K. Batish.
- “Bile Salt Hydrolase activity of some potential indigenous probiotic lactobacilli of human origin” in
International Conference on Traditional Dairy Foods held at NDRI, Karnal (Nov 14-17, 2007).
Authors: Rajesh Kumar and V.K. Batish.
♦ Awards
- Post Doctoral Fellowship has been awarded by Indian Council of Medical Research (ICMR).
- Institutional Senior Research Fellowship awarded during Ph.D programme (Aug 2005- Feb 2009) through
Indian Council of Agricultural Research.
- Awarded best (Ist) poster presentation on “Molecular cloning, sequence analysis and expression of bile salt
hydrolase (bsh) gene from indigenous probiotic Lactobacillus plantarum strain of human origin” in
International Conference on Microbial Biotechnology held at Punjab University, Chandigarh (March
03-04, 2009).
- Awarded second best poster presentation on “Bile Salt Hydrolase activity of some potential indigenous
probiotic lactobacilli of human origin” in International Conference on Traditional Dairy Foods held
at NDRI, Karnal (Nov 14-17, 2007).
♦ Conferences
- Actively Participated in 45th AMI conference held at NDRI Karnal (Nov 23 – 25, 2004).
Personal skills
 - Appreciated by peers and supervisors alike for pioneering attitude, leadership qualities, strong inter-
personal and communication skills
- Fluent in written and spoken English, Hindi.
EXTRA CURRICULAR ACTIVITY
• Delivered presentation and Lectures for students of DNA/VPNR clubs sponsored by DBT, Govt of India.
• Actively participated in as a leading member in annual sports held at NDRI, Karnal (2007, 2008 & 2009).
♦
REFERENCES
♦ Dr. Hemalatha R. (rhemalathanin@yahoo.com)
- Head & Deputy Director, Department of Microbiology & Immunology, National Institute of Nutrition,
Hyderabad, India.
♦ Dr. V.K Batish (vkbatish@gmail.com)
- Head & Pr. Scientist, Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research
Institute, Karnal, Haryana, India
♦ Dr. Sunita Grover (sungro@gmail.com)
- Pr. Scientist, Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute,
Karnal, Haryana, India
♦ Dr. J.K. Kaushik (jaikr1@gmail.com)
- Sr. Scientist, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
♦ Dr. A.K. Mohanty (ashokmohanty1@gmail.com)
- Sr. Scientist, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India

DECLARATION: I hereby declare that the information furnished and entries made in this resume, by me are true to
the best of my knowledge and belief and nothing has been concealed/distorted.

Date: May 24, 2010 (RAJESH KUMAR)

Place: Hyderabad