Escolar Documentos
Profissional Documentos
Cultura Documentos
Department of Food Science and Technology, Chung-Hwa University of Medical Technology, 89, Wenhwa 1st Street, Rende Shiang, Tainan 717, Taiwan, ROC
Department of Baking Technology and Management, National Kaohsiung Hospitality College, 1, Sung-Ho Road, Hsiao-Kang, Kaohsiung 812, Taiwan, ROC
c
Department of Health and Nutrition Biotechnology, Asia University, 500, Lioufeng Road, Wufeng Shiang, Taichung 413, Taiwan, ROC
b
a r t i c l e i n f o
a b s t r a c t
Article history:
Received 18 September 2007
Received in revised form 8 March 2008
Accepted 11 March 2008
The effect of adding grape wine lees (GWL) (50, 100 and 150 g kg1, wet weight basis) on the rheological
and antioxidant properties of ice cream was evaluated to determine the potential of using GWL as
a value-added ingredient in ice cream. Black queen grape was selected because it was the only species for
red winemaking in Taiwan. The addition of GWL decreased the specic gravity, pH, melting rate, rmness, lightness and the amount of freezable water in ice cream, but increased the viscosity, yellowness
and fat destabilization in a dose dependent circumstance. However, the ice cream with high GWL
contents (100 and 150 g kg1) also showed unpleasant effects, such as the decrease of overrun and the
increase of particle size of fat globule. The level of 50 g kg1 was applicable because it improved the
functionality without the disadvantages in the overrun and particle size. Furthermore, GWL signicantly
increased the DPPH radical scavenging activity and reducing power of ice cream, and also its inhibitory
effect toward the oxidation of human erythrocyte membrane. It appeared that the antioxidants in GWL
were quite stable to the process of ice cream making.
2008 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.
Keywords:
Grape wine lees
Ice cream
Antioxidant
Rheological property
1. Introduction
Grape wine lees (GWL) is the sediment in the bottom of the
barrel, about 100 g kg1 of GWL can be obtained from the processing of grape wine. It contains yeast lees and grape stalks, grape
pomace, grape peel and grape seed, so it is considered as a wasteproduct (Salmon, Fornairon-Bonnefond, Mazauric, & Moutounet,
2000). It has been shown that the extract form grape peel could
inhibit the oxidation of human LDL and moderate atherosclerosis
(Celotti, Ferrarini, Zironi, & Conte, 1996; Fitzpatrick, Coffey, &
Jantzen, 1997; Negro, Tommasi, & Miceli, 2003). Negro et al. (2003)
demonstrated that GWL exhibited strong antioxidant activity due
to its high phenolic contents, and proanthocyanidines in grape seed
also had strong antioxidant ability. Goni, Martin, and Saura-Calixto
(2005) found that the microbes in human intestine could
decompose 950 g kg1 total phenolic compounds, 300 g kg1 ber
and 600700 g kg1 proteins in the GWL. The benecial fermentation product, short-chain fatty acid, indicated that GWL could be
utilized by the microbes in human intestine (Toping & Clifton,
2001).
In this study, we used GWL to add the antioxidant properties to
ice cream. In order to determine the potential of using GWL as
0023-6438/$34.00 2008 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.
doi:10.1016/j.lwt.2008.03.008
J.-Y. Hwang et al. / LWT - Food Science and Technology 42 (2009) 312318
313
314
J.-Y. Hwang et al. / LWT - Food Science and Technology 42 (2009) 312318
0 g kg1 GWL
50 g kg1 GWL
100 g kg1 GWL
150 g kg1 GWL
4 g kg1 CMC
Specic
gravity
Viscosity
(mPa s)
pH
Overrun
1.120 0.00a
1.096 0.03b
1.084 0.03c
1.071 0.01d
1.120 0.00a
330 0.00e
640 28.28c
955 91.92b
1390 42.43a
470 14.14d
7.12 0.01a
6.79 0.02b
6.56 0.23bc
6.32 0.11c
7.14 0.00a
53.9 1.8b
51.9 2.5b
41.6 1.3c
35.3 2.1d
60.2 4.6a
J.-Y. Hwang et al. / LWT - Food Science and Technology 42 (2009) 312318
Table 3
Effect of the addition of grape wine lees on the texture prole of ice creams
160
140
0 g kg1 GWL
50 g kg1 GWL
100 g kg1 GWL
150 g kg1 GWL
4 g kg1 CMC
120
Weight (g)
Firmness (g)
Cohesiveness
291 35a
173 29b
142 19bc
113 1c
251 11a
0.25 0.01a
0.23 0.01a
0.23 0.03a
0.16 0.02b
0.29 0.04a
100
80
reductions in the rmness were found at 50, 100 and 150 g kg1
GWL, respectively. However, the cohesiveness was less affected by
GWL. Our data showed that the cohesiveness did not change at 50
and 100 g kg1 GWL. For 150 g kg1 GWL, about 36% decline in the
cohesiveness was found. Table 4 shows the color properties of ice
cream inuenced by the addition of GWL. As expected, the addition
of GWL increased the redness and yellowness of ice creams, but
decreased the lightness.
60
40
20
315
20
40
60
80
100
120
140
Time (mins)
Fig. 1. Effect of the addition of GWL on ice cream melting. The values are mean and the
error bar represents standard deviation (A: control; -: 50 g kg1 GWL; :: 100 g kg1
GWL; B:150 g kg1 GWL; J: 4 g kg1 CMC).
0 g kg1 GWL
50 g kg1 GWL
100 g kg1 GWL
150 g kg1 GWL
4 g kg1 CMC
Fat destabilization
index (%)
Particle size of
fat globule (nm)
10.3 1.6d
16.4 2.1cd
24.6 1.0bc
32.5 8.0b
49.5 4.4a
198 15c
221 19c
448 76b
587 95a
197 14c
0 g kg1 GWL
50 g kg1 GWL
100 g kg1 GWL
150 g kg1 GWL
4 g kg1 CMC
96.4 0.7a
68.3 1.4b
67.8 0.7b
59.4 0.5c
96.2 0.4a
0.2 0.1d
2.3 0.0c
4.3 0.1b
5.4 0.2a
3.4 0.0e
2.3 0.3e
8.0 0.2d
10.7 0.3b
9.6 0.3c
11.5 0.2a
316
J.-Y. Hwang et al. / LWT - Food Science and Technology 42 (2009) 312318
The total phenolic contents of the ice creams with the addition
of GWL in the range of 0150 g kg1 are shown in Fig. 4. It was
found that the total phenolic contents of ice cream and GWL were
1.52 and 7.51 mg/ml, respectively. The addition of GWL resulted in
the increase of total phenolic content. When adding 150 g kg1
GWL, the total phenolic content reached 3.58 mg/ml. We estimated
the amount of total phenolic content in ice cream with 150 g kg1
GWL was 2.42 mg/ml (0.85 1.52 mg/ml 0.15 7.51 mg/ml).
Thus, like the total anthocyanin content, the total phenolic content
also did not decrease due to the processing of ice cream. When
converting the unit to mg/g of GWL, we obtained the value of total
phenolic content of 45 mg/g GWL, about 5% of GWL. As expected,
our data indicated that the GWL still contained great amount of
phenolic compounds. Our results indicated that both anthocyanins
and phenolic compounds in the GWL were quite stable during the
process of ice cream making. Therefore, the addition of GWL could
increase the antioxidant activity of ice cream.
-0.05
-0.1
-0.15
-0.2
-0.25
-0.3
-25
-20
-15
-10
-5
Temperature (C)
Fig. 2. Effect of the addition of grape wine lees on the ice crystal-melting of ice creams
measured by differential scanning calorimetry (DSC) (A: control; -: 50 g kg1 GWL;
:: 100 g kg1 GWL; B:150 g kg1 GWL; J: 4 g kg1 CMC). Enthalpy (DH ) for the ice
crystal-melting of 0 g kg1 GWL, 50 g kg1 GWL, 100 g kg1 GWL, 150 g kg1 GWL and
4 g kg1 CMC was 7.16, 6.73, 6.25, 4.43 and 9.76 J/g, respectively.
DPPH is a stable free radical that would be scavenged by a proton-donating substance, for example, an antioxidant (Espin, Rivas,
& Wichers, 2000). Based on this principle, the antioxidant activity
of a substance can be expressed as its ability in scavenging the
DPPH free radical. Fig. 5 shows the effect of GWL on the DPPH
radical scavenging activity of ice cream. The DPPH radical scavenging activity of GWL (0.17 g) was equivalent to 1 mg butylated
hydroxyanisole (BHA). Adding GWL signicantly increased the
DPPH radical scavenging activity; however, the amount of addition
did not differ in the range of 50150 g kg1.
The reducing powers of ice creams with the addition of GWL are
shown in Fig. 6. The reducing power of GWL (0.17 g) was equivalent
to 0.5 mg BHA. No reducing power was detectable in the ice cream
without GWL or with 4 g kg1 CMC. Addition of GWL, however,
increased the reducing power of ice cream increased with the
amount of GWL. When compared to the proles of total anthocyanins and phenolic contents, it was noted that the prole of
reducing power is more similar to that of total anthocyanin content
than total phenolic content. Therefore, the reducing power
appeared to be inuenced by anthocyanins in GWL.
9
700
500
400
300
200
c
100
7
6
5
4
b
bc
c
3
2
d
e
ND
0 g kg-1
GWL
600
ND
50 g kg-1
GWL
4 g kg-1
CMC
0
Wine lees
Fig. 3. The total anthocyanin contents of ice creams containing grape wine lees in the
range of 0150 g kg1 (ND: value was not detected).
0 g kg-1
GWL
50 g kg-1
GWL
4 g kg-1
CMC
Wine lees
Fig. 4. The total phenolic content of ice creams containing grape wine lees in the range
of 0150 g kg1.
J.-Y. Hwang et al. / LWT - Food Science and Technology 42 (2009) 312318
100
90
317
80
70
80
70
60
b
b
50
60
40
50
40
30
30
c
20
20
c
c
10
10
0
0
0 g kg-1
GWL
Wine
lees
BHA
1 mg
0 g kg-1
GWL
4 g kg-1
CMC
Wine
lees
BHA
1 mg
Fig. 5. The DPPH scavenging activity of ice creams containing grape wine lees in the
range of 0150 g kg1.
Fig. 7. The inhibitory effect of ice creams containing grape wine lees in the range of 0
150 g kg1 toward the oxidation of human erythrocyte membrane.
2.5
Absorbance at 700 nm
1.5
b
1
4. Conclusions
The addition of GWL increased the melting rate, fat
destabilization and decreased the freezable water of ice cream.
However, ice cream with high GWL contents also showed
unpleasant effects, such as the decrease of overrun and the increase
of particle size of fat globule. These unwanted effects were minor at
low GWL concentration. For example, no signicant decrease of the
overrun and increase of the particle size were found at 50 g kg1
level. GWL contains a signicant amount of phenolic compounds
and may be used to produce value-added food products. In this
study, we demonstrated that the addition of GWL could signicantly increase the antioxidant activity of ice cream, and the antioxidant in the GWL appeared to be quite stable during the process
of ice cream making. Therefore, GWL has the potential to be used as
a value-added ingredient in ice cream industry to enhance the
antioxidant activity.
References
0.5
ND
0
ND
-1
0 g kg
GWL
-1
50 g kg
GWL
-1
-1
100 g kg 150 g kg
GWL
GWL
4 g kg-1
CMC
Wine
lees
BHA
0.5 mg
Fig. 6. The reducing activity of ice creams containing grape wine lees in the range of
0150 g kg1 (ND: value was not detected).
318
J.-Y. Hwang et al. / LWT - Food Science and Technology 42 (2009) 312318