Escolar Documentos
Profissional Documentos
Cultura Documentos
Candidate
Number
Name
9747 / 01
HIGHER 2
Paper 1 Multiple Choice
02 SEPT 2009
1 hour 15 minutes
Additional Materials:
INSTRUCTIONS TO CANDIDATES
Write your name, Centre number and candidate number on the multiple choice answer sheet
provided.
There are forty questions in this paper. Answer all questions. For each question there are four
possible answers, A, B, C and D. Choose the one you consider correct and record your choice
in soft pencil on the separate answer sheet.
Read very carefully the instructions on the answer sheet.
INFORMATION FOR CANDIDATES
Each correct answer will score one mark. A mark will not be deducted for a wrong answer.
Any rough working should be done in this booklet.
Calculators may be used.
9747/01/Prelim 09
[Turn over
2
1
An enzyme is a globular protein held together by several different types of bonds, giving
the enzyme primary, secondary and tertiary levels of structure.
Which combination correctly summarises the types of bond involved in each level of
structure?
Disulphide bonds
Hydrogen bonds
Ionic bonds
Peptide bonds
Primary
Tertiary
Secondary
Tertiary
Primary,
tertiary
Primary
Secondary,
tertiary
Secondary
Secondary
Secondary
Tertiary
Primary
Tertiary
Secondary,
tertiary
Tertiary
Primary
9747/01/Prelim 09
[Turn over
3
3
In an experiment using starch and amylase, the concentrations of starch and maltose
present in the reacting mixture are measured every minute for 20 minutes. 1%
hydrochloric acid is added after 10 minutes and the mixture is heated to 60C at 14
minutes.
Which graph represents the results of this experiment?
Anchorage independence
Contact inhibition
Metastasis
Stimulate angiogenesis
9747/01/Prelim 09
[Turn over
4
5
The figure below shows a hypothetical diploid cell with six chromosomes.
II
III
IV
VI
I and IV only
I, V and VI only
9747/01/Prelim 09
[Turn over
5
6
The figure below represents the relative amount of DNA per nucleus during several cell
divisions in a plant tissue.
DNA per
nucleus/
arbitrary
units
Enhancer
Activator
RNA polymerase
Promoter
Activator
RNA polymerase
Enhancer
Activator
Mediator
Promoter
Silencer
Mediator
9747/01/Prelim 09
[Turn over
6
8
Five different amino acids (numbered 1 to 5 below) form the following sequence in part
of a polypeptide chain:
2------3------4------1------3------5------2
Messenger RNA (mRNA) codons which correspond to these amino acids are:
amino acid 1
amino acid 2
amino acid 3
amino acid 4
amino acid 5
UGU
GAU
CAC
CAA
AAG
Which one of the following DNA base sequences could provide the code for the given
section of polypeptide?
A
CTAGTGGTTACAGTGTTCCTA
CTAGTGGTTACAGTGTTGCTA
CTAGTGGTTTCTGTGTTGCTA
CTAGTGGTTACAGTGTTCCTT
9747/01/Prelim 09
[Turn over
7
10
11
Hemagglutinin
Matrix protein
Neuraminidase
Nucleoproteins
The diagram represents the structure of the human immunodeficiency virus (HIV).
Attaches virus
to target cells
Fuses with
membrane of
target cells
Protects the
viral genetic
material
Synthesises
single-stranded
DNA from viral
genetic material
Attaches virus
to target cells
Fuses with
membrane of
target cells
Protects the
viral genetic
material
Synthesises
double-stranded
DNA from viral
genetic material
Attaches virus
to target cells
Protects the
viral genetic
material
Fuses with
membrane of
target cells
Synthesises
single-stranded
RNA from viral
genetic material
Fuses with
membrane of
target cells
Protects the
viral genetic
material
Attaches virus
to target cells
Synthesises
double-stranded
RNA from viral
genetic material
9747/01/Prelim 09
[Turn over
8
12
Which of the following accounts for the living S cells in the mouse blood sample, even
though the original injection mixture contained only heat-killed S cells and living R cells?
13
Binary fission
Conjugation
Transduction
Transformation
A scientist inserts the gene for human growth factor into the Escherichia coli lac operon,
replacing the structural genes with the gene for human growth factor. What substance
must be added to the culture of bacteria in order to cause them to produce the human
growth factor?
A
Lactose
Operator protein
Repressor protein
9747/01/Prelim 09
[Turn over
9
14
15
The role of telomerase is to maintain the length of telomeres in germline cells. Which
statement is true about how telomerase works?
A
It has RNA polymerase that can add ribonucleotides to the parental strand.
16
17
Which of the following is not a requirement for the mechanism of RNA splicing?
A
Introns
An investigator wanted to determine the function of protein X. He cloned the gene X into
plasmid 1. He then set up another plasmid (plasmid 2) with a binding site for the product
9747/01/Prelim 09
[Turn over
10
of gene X plus a reporter gene. A reporter gene codes for the synthesis of a product that
is easily measured. The two plasmids (shown below) were then introduced into the
nucleus of a host cell.
He noted that there was an increase in the number of reporter gene transcripts. Which of
the following statements is a valid conclusion regarding gene X or product X?
18
19
A girl has blood group A and her brother has blood group B. Which combination of
genotypes cannot belong to their parents?
Mother
Father
9747/01/Prelim 09
[Turn over
11
20
21
IA IA
IB IO
IA IB
IA IB
IO IO
IA IB
IB IO
IA IO
In one of the experiments to test the 3:1 ratio of Mendels laws, 705 plants are
observed to have red flowers and 224 had white flowers. The P (probability) value,
associated with the calculated chi-square value of 2.668, is 0.10, under the 95%
(P=0.05) confidence level. The P refers to
A
the chance that there is a 10% probability that any deviation from the expected
result is due to a random event only.
the chance that there is a 10% probability that any deviation from the expected
result is significant.
the chance that there is no difference between the expected and observed
results.
the chance that there is a difference between the expected and observed
results.
Fruit flies homozygous for long wings were crossed with flies homozygous for vestigial
wings.
The F1 generation was raised at 3 different temperatures. The F1 flies were then
crossed with each other to produce the F2 generation. The results of the F1 and F2
generation are shown below.
Temperature
21C
26C
31C
F1 result
All long wings
All long wings
All long wings
F2 result
3/4 long wings, 1/4 vestigial wings
3/4 long wings, 1/4 intermediate wings
All long wings
22
Heterozygous flies have vestigial wings only at 21C and below, but have long
wings at 31C and above.
Long wing allele is dominant at higher temperatures, but vestigial wing allele is
dominant at lower temperature.
Vestigial wing allele is recessive but causes a vestigial wing phenotype only at
lower temperatures.
The coat colour of Labrador retrievers is controlled by two genes, B and E. Allele B
(dominant) codes for black coat, while allele b (recessive) codes for brown coat. The
coat colour of a Labrador retriever with genotype ee is yellow.
9747/01/Prelim 09
[Turn over
12
A cross between Labrador retrievers with the genotype EeBb and EeBB was
performed. What is the expected phenotype in the offspring of this cross?
23
24
Continuous variation is controlled by two or more genes and the traits are
qualitative.
Discontinuous variation shows traits that are controlled by one or two genes
and is not influenced by environmental factors.
14
25
1
2
3
A
GP
RuBP
Sugar
B
GP
Sugar
RuBP
C
RuBP
GP
Sugar
D
RuBP
Sugar
GP
When cyanide is bound to cytochrome oxidase, the cell can no longer produce ATP
aerobically. Which statement below best explains this?
A
It prevents NADPH from being oxidised to NADP+, hence preventing electron flow
down the electron transport chain.
9747/01/Prelim 09
[Turn over
13
26
It prevents oxygen from accepting electrons and protons to form water, hence
preventing electron flow down the electron transport chain.
Which structure allows the diffusion of Cl ions between the outside and inside of the
cell?
A
A only
B only
A and B
C and D
27
The
below shows a
action potential.
diagram
normal
9747/01/Prelim 09
[Turn over
14
Which events are occurring at positions 2 and 4 to produce the illustrated action
potential?
Position 2
28
29
Position 4
Which of the following represents the changes that insulin brings about in the
appropriate target cells?
9747/01/Prelim 09
[Turn over
15
30
Rate of
glycogenolysis
Rate of
glycogenesis
Level of
cAMP
Increase
Decrease
Increases
Decrease
Increase
Increases
Increase
Decrease
Decreases
Decrease
Increase
Decreases
Which one of the following processes deviates from the chain of events as shown
above?
31
Growth factors, protein molecules released by actively growing cells, stimulate cell
growth and cell division in adjacent cells.
In which of the following scenarios would allopatric speciation occur most quickly?
A
9747/01/Prelim 09
[Turn over
16
32
A population of giraffes free to roam the entire nature reserve after the artificial
removal of all predators.
The table below shows the number of amino acid differences in the alpha haemoglobin
chains between the crocodile and various organisms.
Crocodile
Lungfish
Coelacanth
Shark
Goldfish
85
78
77
70
Coelacanth
Goldfish
Lungfish
Shark
9747/01/Prelim 09
[Turn over
17
33
34
35
During the drought years on the island of Daphne Major, small seeds became rare,
leaving behind only large seeds as a food source for birds. In the event of a prolonged
drought lasting several years, which of the following could be a possible observation?
A
Large-beaked birds eating less, leaving food available for the small-beaked birds.
Small in size.
If the first three nucleotides in a six-nucleotide restriction site are CTG, what would the
next three nucleotides most likely be?
A
CAG
CTG
GAC
GTC
9747/01/Prelim 09
[Turn over
18
36
Genomic DNA
M
WT
RNA
mutant
600
600
468
468
442
442
411
411
368
368
267
267
WT
mutant
37
Which of the following technique does not involve any nucleic acid hybridisation?
A
DNA fingerprinting
Gel electrophoresis
Southern blotting
9747/01/Prelim 09
[Turn over
19
38
39
40
Gene therapy is the insertion of a therapeutic gene into a target cell with nonfunctional genes. Which of the following are techniques of gene therapy to treat a
genetic disease?
I
II
III
A therapeutic gene enters the cell, but is not inserted into the genome.
I, II and IV only
I and II only
II and IV only
- End of paper -
9747/01/Prelim 09
[Turn over