The paraffin Embedding System allows a convenient, quick and careful paraffin

embedding of tissue specimens. The modular structure and the standard height of the
working area permit to combine the three modules of the embedding Station so as to meet
the needs of the user. All function cloud be run and checked separately. Each heating and
cooling functions are controlled by accurate digital thermostats (heating up + 70 C,
cooling down to -20 C). Marble working platform is easy to clean up.
Paraffin Dispenser:
Stainless steel paraffin large tank of 5 liters capacity.
The sensor allows to supply paraffin without any mechanism (and without
maintenance). Halogen lamp to light up the working area.

Cooled spot for a quick cooling of specimens. Wide aluminum hot plate, with 6 holes
for forceps.
Heated basin to collect the excess of paraffin. Possibility to apply heated forceps
directly to the Dispenser unit.
Technical features:

Dimensions overall: 900x500x360 mm (WxDxH)

Weight: 53 kg

Working Voltage: 220 V / 50 Hz((110V / 60 Hz, optional)

Power: < 1000 watt.

Accessories: Foot Switch and Forceps Heating Station (with internal power supply
preinstalled)

Thermal Unit:

Stainless steel upper chamber for paraffin moulds and lower basin for liquid
paraffin ( to store samples from tissue processor) are heated and regulated
separately.

The stainless steel basin is removable in order to facilitate cleaning operations.

Cooling Plate:

The cooling plate can be used either for embedding or during microtome cutting
operation.

The cooling plate has a new frame all around the stainless steel surface which
improved cooling performances and prevents the dropping of water around the
instrument.

Xylene I (10 minutes)

Xylene II (10 minutes)
Xylene III (10 minutes)
100% Alcohol (5 minutes)
100% Alcohol (5 minutes)
95% Alcohol (5 minutes)
90% Alcohol (5 minutes)
80% Alcohol (5 minutes)
70% Alcohol (5 minutes)
Running tap water (rinse 5 minutes)

Hematoxylin/Alugbati stain (10 minutes)

Running tap water (quick rinse)
Acid Alcohol (1 to 2 dips)

Running tap water (quick rinse)

Ammonia water (rinse 10x)
Running tap water (rinse 10 minutes)
Eosin (5 minutes)
70% Alcohol (2 to 3 dips)
Running tap water (quick rinse)
80% Alcohol (2 to 3 dips)
95% Alcohol (5 minutes)
80% Alcohol (2 to 3 dips)
100% Alcohol (10 minutes)
95% Alcohol (5 minutes)
Xylene I (2 minutes)
100% Alcohol (10 minutes)
Xylene II (2 minutes)
Xylene I (2 minutes)
Xylene III (2 minutes)
Xylene II (2 minutes)
Xylene III (2 minutes)

The staining procedure is adapted from Harris hematoxylin staining protocol

of Ramakrishan (2012) and H&E staining procedure of Nagy (2008).

F2

ASSIGNMENT IN
HISTOPATHOLOGY
(LAB)

EMBEDDING MACHINE
SCHEMATIC DIAGRAM OF TISSUE STAINING

Bless Marie B. Andaya

BSMT 4-2
Maam Isolde M. Quitan