Scribd Logo
Fazer o upload

Bem-vindo(a) ao Scribd!

  • Facilitated Transport of Uracil and 5 Fluorouracil, and Permeation of Orotic Acid Into Cultured Mammalian Cells

    Enviado por

    Hitesh Verma
    14 visualizações1 página
    abstract orotic acid

    Título original

    1

    Direitos autorais

    © © All Rights Reserved

    Formatos disponíveis

    PDF, TXT ou leia online no Scribd

    Você considera este documento útil?

    Este conteúdo é inapropriado?

    Denunciar este documento
    abstract orotic acid

    Direitos autorais:

    © All Rights Reserved
    Baixe no formato PDF, TXT ou leia online no Scribd
    Sinalizar o conteúdo como inadequado
    14 visualizações1 página

    Facilitated Transport of Uracil and 5 Fluorouracil, and Permeation of Orotic Acid Into Cultured Mammalian Cells

    Enviado por

    Hitesh Verma
    abstract orotic acid

    Direitos autorais:

    © All Rights Reserved
    Baixe no formato PDF, TXT ou leia online no Scribd
    Sinalizar o conteúdo como inadequado
    de 1

    22/10/2016

    Facilitatedtransportofuraciland5fluorouracil,andpermeationoforoticacidintoculturedmammaliancells.PubMedNCBI

    PubMed

    NCBIwillbetestinghttpsonpublicwebserversfrom1:004:00PMEDT(17:0020:00UTC)onMonday,
    Fulltextlinks
    October24.YoumayexperienceproblemswithNCBIservices,especiallyfiledownloads,duringthattime.
    Pleaseplanaccordingly.Readmore.

    Format:Abstract
    JCellPhysiol.1980Sep104(3):30919.

    Facilitatedtransportofuraciland5fluorouracil,andpermeationof
    oroticacidintoculturedmammaliancells.
    WohlhueterRM,McIvorRS,PlagemannPG.

    Abstract
    Themodeofpermeationofuracil,5fluorouracil,andoroticacidintocellshasbeeninvestigatedin
    fourestablishedcelllines(Novikoffrathepatopma,P388mouseleukemia,mouseL.,and
    Chinesehamsterovarycells)inattemptstoassesstheratedeterminingstep(s)intheir
    incorporationintothenucleotidepoolandnucleicacids.Uraciland5fluorouracilshareda
    saturabletransportsystem(Km=5to15mM)capableofrapidequilibrationofthesesubstrates
    acrossthecellmembrane(t1/2at25degreesinfirstorderrangeofconcentration=25to58
    sec).Thusitseemsunlikelythattransportislimitingtheincorporationofuracilorfluorouracil.
    Theirtransportwasinhibitedbyvariousnucleosidesandhypoxanthine.Onlythenonionizedform
    offluorouracilwasasubstrateforthetransporterexclusionofchargedpyrimidinesmayexplain
    whyorotatewasnotasubstrateatphysiologicalpH.Orotatepermeatedthecellmembranemuch
    moreslowly(t1/2=2890to6930sec)itspermeationwasapparentlynonmediatedandrate
    determiningintheconversionofextracellularorotatetointracellularnucleotides.
    PMID:7419607 DOI:10.1002/jcp.1041040305
    [PubMedindexedforMEDLINE]

    PublicationTypes,MeSHTerms,Substances,GrantSupport
    LinkOutmoreresources

    PubMedCommons

    PubMedCommonshome

    0comments
    HowtojoinPubMedCommons

    https://www.ncbi.nlm.nih.gov/pubmed/7419607

    1/1

    Você também pode gostar