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AnnonaSquamosa(atis)leavesasmolluscicideforGoldenapplesnailLawEssaysandPapers
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AnnonaSquamosa(atis)leavesasmolluscicideforGoldenapplesnailLawEssaysandPapers
more information about the other uses of Annonasquamosa, for example, as a molluscicide. Farmers. This study can help farmers in having aordable
and eective molluscicide against pests on their farm lots.
Department of Agriculture. Instead of letting Atis seed go to waste these can be made useful by making it into an eective molluscicide. They can use
Atis as one of their products in agriculture and make it known into other places. Growers of Atis. This study will help tose who are planting Atis to have
new source of income.
The Society. It will make them aware of the availability of the natural organic insecticide that is less expensive yet safe and eective. Future
Researchers. It will serve as a reference material for their future research. Scope and Delimitation
This study aims to control the mortality rate of Golden Apple Snail by using the extract of Annona squamosa(Atis) seed. The Golden apple snails which
were usually present on farms are involve in this study, because those snails would be the tester on the eectiveness of the Annona squamosa seed
extract Molluscicide.
This study will be conducted in Naga City Science High School for the school year 2012- 2013 and the researchers as the observers. The aspects
looked into where this research would be possible for control for the said pest, the problem and the said methods for treating them. The study on
Annona squamosa seed as molluscicide for Golden Apple Snail limits on the eectiveness of the extract of the seed. It does not include the
appearance of the mixture. DeFnition of Terms
Molluscicide. It is a solution that can kill molluscs.
Atis seeds. It will be the main ingredient of the AtisMolluscicide. It has toxic that can kill the Golden Apple Snail. Mortality. This refers to the death rate
of the Golden Apple Snail. Potency. It determines the eectiveness of the Atis Molluscicide. Research Paradigm
Figure1. Research Paradigm
Chapter II
REVIEW OF RELATED LITERATURES AND RELATED STUDIES
Related Literature
Golden Kuhols came from South America. It was introduced in Taiwan as a food. It became an aquarium pet and helps in cleaning tanks. It multiplied
rapidly until it reaches the waterways. (2008) (http://juanmagsasaka.blogspot.com/2008/08/protect-rice-seedlings-against-golden.html) It was Frst
introduced in Asia in the year 1980 with expectations that it can be a high-protein food source. However, it has invaded the rice Felds in Asia. The
young rice seedlings were eaten by these snails. (http://www.jstor.org/discover/10.2307/4314515?uid=3738824&uid=2&uid=4&sid=21100870152841)
Annona squamosa or known as sugar apple is the most widely grown of all the species of Annona. When eating this fruit, the seeds are always left out
due to its toxicity. (http://www.ehow.com/facts_6947915_sugar-apple-fruit.html).
The powdered seeds as well as the pounded leaves are used as insecticide in India. The heat-extracted oil from the seeds can be used against
agricultural pests. (http://www.oocities.org/athens/academy/4059/atis.html). It can also be used as a lice remover; however, it must not reach the eyes
because
it
can
cause
blindness.
If
it
is
applied
to
the
uterus,
it
can
cause
abortion.
(http://www.hort.purdue.edu/newcrop/morton/sugar_apple.html#Toxicity)
The seeds of A. squamosa contain annonaceous acetogenins which have cytotoxic activities. (http://www.globinmed.com/index.php?
option=com_content&view=article&id=83499:annona-squamosa&catid=703:a). It is said in one study that the acetogenins from the seed are more
toxic than the synthetic pesticide. (http://www.stuartxchange.org/Atis.html)
Molluscicides are used to control slugs and snails. Slugs and snails belong to the mollusks group. Metaldehyde is one of the most commonly used
active ingredients in molluscicides. Molluscicides can be manufactured as a colored pellet or a spray. Since molluscicides are commonly chemicals,
there are other ways to control mollusks. This includes using traps or barriers. There are slug and snail baits that contain iron phosphate as the active
ingredient. Iron phosphate is less toxic than metaldehyde. (http://toxipedia.org/display/toxipedia/Biological+Properties+of+Molluscicides).
Copper Sulfate can be used as molluscicide. It has been used for about 40 years now. It is still used as molluscicide in some areas. Although it is
eective, it still has limitations; it is more eective in other habitats. (http://whqlibdoc.who.int/monograph/WHO_MONO_50_(part4).pdf) Currently, only
copper sulfate and copper chelate formulations have been utilized as a viable molluscicide treatment in open water systems with proper permitting.
Niclosamide
may
be
used
for
controlling
snail
(mollusks)
populations
in
aquaculture
ponds.
(http://www.glmris.anl.gov/documents/docs/anscontrol/Molluscicides.pdf)
According to Dr. Eduardo Quisumbing, the seed area is considered a powerful irritant to the conjunctiva; the roots are considered a drastic purgative.
So considering the parts of the articles stated above it would be a great help in proving the researchers study.
(http://www.pinoyecofarmer.com/2010/10/21/atis-leaves-developed-antidengue-so
lution/) Related Studies
Study shows that Annona squamosa plant can be used as a molluscicide. Among the seeds, bark and the leaves of the plant, it was observed that the
highest molluscicidal activity of the Annona squamosa plant is in the seed extracts. The acetogenins extracted from the seeds of the plant were more
toxic than synthetic pesticicides.
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The combination of the powdered seed of A. squamosa, the oil from the cedar, Cedrus deodara Roxh., and from neem, Azadirachta indica A. Juss.,
have more toxic than the separate constituents of the cited plants. (Singh & Singh , 2001) Another study was conducted about the eects of the A.
squamosa Linn. and Lawsonia inermis Linn. (Henna) and their combinations with other herbal molluscicides, and acetogenins extracted from the
seeds of A. squamosa Linn., on the reproduction of Lymnaea acuminata.
It was observed that the plant- derived molluscicides aected the mortality rate of the snails. However, when the snails were put back to the
freshwater, it regained its capability to reproduct. (Singh and Singh, 2004) A study was performed, by other proponents, to know the molluscicidal
properties of some species of Annona. There were six species used in this study and were observed if they have the capability to cause death of
Biomphalaria
glabrata.
Biomphalaria
glabrata
is
tropical
freshwater
snail
indigenous
to
ponds,
marshes
and
streams.
(http://biology.unm.edu/biology/esloker/pi/WhatIsBiomphalaria_glabrata.pdf)
The results of the experiments showed that the majority of the extracts of the Annona species have the properties to harm the Biomphlaria glabrata,
moreover, the Annona muricata L. (leaf) and Annona squamosa Veil. (root) was given emphasis on because of their additional toxicity towards the snail
eggs. (Dos Santos AF, SantAna AE., 2001) Other fruits were tested to know if they have a molluscicidal activity. A combination of jackfruit, durian and
breadnut peels was studied to control Golden Kuhol. The researcher used the rotten peels of the said fruits in making the alternative molluscicide. The
study has 5 treatments and each treatment composes of dierent masses of the dierent fruits.
The researcher added 45 g and 10 g to the fourth and Ffth trial respectively. It is stated in the study that the jackfruit peel have a foul smell that can kill
the Golden Kuhol. (Villa Agustin, 2012) (http://www.scribd.com/emmanuel_agustin_2/d/79325542-The-eect-of-jackfruit- durian-and-breadnut-peelscan-control-golden-apple-snail#) Barringtonia racemosa fruit and seed extracts were experimented to know if it has a molluscicidal, cercariacidal,
larvicidal, and antiplasmodial properties. The extracts of the stem bark is said to be toxic to mosquitoes, snails and brine- shrimp. The World Health
Organizations method for examining molluscicides was used in this study. Biomphalaria pfeieri snails were collected from a stream in Edun-Abon in
Nigeria.
The snails were separately exposed in the concentrations of the B. racemosa fruit and seed aqueous extracts. Niclosamide is a chemical molluscicide
that also used in this experiment as the control molluscicide. There were 10 snails used per extract or niclosamide dose and was exposed within 24
hours. It has three replicates. After that, the observations were recorded. The fruit extract of B. racemosa has stronger molluscicidal activity than the
sed extract of the plant. (http://redalyc.uaemex.mx/pdf/856/85630505.pdf) Chaper III
METHODOLOGY
Research Method
This study will use the experimental method of research. In this research method, the researchers will manipulate the variables involve in the study.
The purpose of this method is to determine the eectiveness of Annona squamosaas Molluscicide for Golden Kuhol. Set Up
There will be 4 set ups: for 50% concentration, 75% concentration, 100 % concentration of Annona squamosa (atis) molluscicide, and for commercial
molluscicide. In each set up, there will be 3 kuhols. Then, the researchers will conduct an observation about the eect of the product and its dierence
to the commercial molluscicide. Materials Needed
In making the Annona squamosa (atis) as molluscicide for golden kuhol the following materials are needed: Annonasquamosa (Atis)seeds
Distilled water
Spray bottles
Strainer/ Cloth\
Copper sulfate
Mortar and Pestle
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