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Research Article
ISSN: 0974-6943
INTRODUCTION
Benign prostatic hyperplasia (BPH) is age related disorder where there is
nonmalignant enlargement of the prostate due to excessive cellular growth
of both the glandular and the stromal elements of the gland. BPH is usually
associated with Lower Urinary Tract Symptoms (LUTS) which may present
with filling symptoms (frequency, urgency, and urge incontinence) or voiding
symptoms (hesitancy, poor urinary stream, straining, intermittent stream,
and a feeling of incomplete bladder emptying), or both including complaints
of nocturia (night-time voiding of urine). One-third of men over 50 years
old reported to develop some form of LUTS with 25% of these requiring
surgeries (transurethral resection) [1-5].
Prostate being an endocrinal dependent organ, hormonal imbalance; aging
and androgens are main cause for the development of BPH. Hence utilization
of androgen deprivation drugs affecting hypothalamicpituitarygonadal
axis like gonadotropin-releasing hormone (GnRH) analogues, anti-androgens,
and 5-reductase inhibitors decreases the size of the prostate and the
resistance to outflow through the prostatic urethra[6-8].
Prostatitis of different form is associated with BPH. It may be infectious or
non infectious prostatitis. Inflammatory cells and mediators like leucotrines,
prostaglandins, and thromboxanes etc are found in prostatic secretion.
Increase Urinary tract infections (UTIs) are frequently observed with BPH
and may be related to the amount of urinary retention due to BPH
conditions[9].
Some of the plants like the liposterolic extract of Saw Palmetto fruits
(Serenoa repens, or Sabal serulata) ; [10] extracts of Stinging Nettle root
(Urtica dioica L.); [10] the extract of Pygeum africanum bark; [11] have been
used, singly or in combination with other botanicals for the condition of
BPH. Ayurvedic formulation Chandraprabha vati is an important therapeutic
medicine recommended in BPH conditions.
Chandraprabha vati is a polyherbal formulation where each herb is mixed
and processed in specific manner. It is used for Samana Chikitsa (alleviative
treatment) or symptomatic treatment. It is a good Rasayana (rejuvenator)
useful in all three doshas. It opens channels and improves mobility of
Doshas. More specifically, it is used for genito-urinary ailments, muscular
*Corresponding author.
Mr. Rahul K. Dumbre
Plot No 430, Sector No 28
Nigdi Pradhikaran,
Pune-411044,M.H.India.
& joint pain, obesity, and cellulites[12-13]. Present study is done to understand
the effect of Chandraprabha vati in experimental prostatic hyperplasia and
inflammation in rats.
MATERIALS AND METHODS
Chemicals and Formulation
Chandraprabha vati formulation containing following contents was purchased
of Divya Pharmacy, Haridwar, India
Formula as per the B.R. (Bhaisajya Ratnavali)
Sr. No.
Name of Ingredient
% content
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
0.92
3.66
3.66
3.66
3.66
7.34
14.68
7.34
29.36
0.92
0.92
3.66
5302-5304
Dose
(mg/kg)
B.W. (g)
Control
Negative
Finasteride
Chandraprabha
vati (CV)
Water
5
100
200
400
26614.3
27211.5
26310.7
26113.5
25914.8
26110.5
Volume of
prostate (cm3)
0.290.05**
0.710.03
0.320.07**
0.470.05**
0.440.08*
0.490.02*
12914.3**
3117.3
1589.7**
26510.6*
24011.3**
18711.9**
n=6, meanS.E.M. * p, <0.05, ** p, <0.01 vs.Negative Group. B.W. represents body weight.
Histopathology of prostates of negative control show significant gland
crowding, epithelial hyperplasia, fibrovascular proliferation in stroma and
decreased acini diameter as compared to normal control. Significant reduction
in gland proliferation, epithelial and cellular hyperplasia and stromal
proliferation at dose of 100, 200, 400 mg/kg for Chandraprabha vati compared
to negative control was observed. The effect was more pronounced at
400mg/kg dose that was comparable with positive control Finasteride (Table
2)
Table 2: Results of histopathology of prostate glands.
Group
Gland
crowding
Epithelial
Stromal
Stromal
hyperplasia hyperplasia inflammation
Normal Control
Testosterone control
Standard
(Finasteride 5mg/kg +testosterone)
Test Group)
(100 mg/kg CV+ testosterone
Test Group
(200 mg/kg CV + testosterone)
Test Group
(400mg/kg CV) + testosterone)
+++
++
++
+
+++
+
++
++
++
++
+
+++
:- Mild Hyperplasia
:- Severe Hyperplasia
++ :- Moderate Hyperplasia.
:- Absent Hyperplasia.
Statistical Analysis
The data was expressed as mean SEM. The results were analyzed
statistically using ANOVA and students t test. The minimum level
significance was considered as P<0.05
Control
Chandraprabha
vati (CV)
Indomethacin
RESULTS
21 days s.c injection of testosterone at dose of 0.5mg/0.1 ml significantly
increased prostate weight and volume of castrated animals compared to
normal animals. Chandraprabha vati, at all doses 100, 200, 400mg/kg, showed
effect on volume and weight of prostate after 21 days treatment. Both
Chandraprabha vati at dose of 400mg/kg and positive control Finasteride at
5mg/kg significant and comparable reduction in prostate volume and weight
compared to testosterone treated (negative control) animals (Table 1).
Dose
mg/kg
orally
100
200
400
10
2hr
45.24.0
36.73.1
29.33.9*
21.03.1**
22.23.2**
3hr
59.02.6
42.12.4**
33.04.1**
25.13.9**
20.61.7**
DISCUSSION
Benign Prostatic Hyperplasia is a complex disease where various mechanisms
are leading to enlargement of tissue and retention of urine. Age related
hormonal imbalance of testosterone/estrogen and activity of testosterone
and dihydrotestosterone after binding to cellular androgen receptors set
complex secondary reactions that signal the cell to produce growth factors
resulting in hyper plastic growth of prostate. High amounts of 5 alpha
reductase enzyme activity that convert testosterone to dihydrotestosterone
(active form of androgen in prostate) in prostate as well as abundance of
estrogen receptor are responsible for BPH prostates[16-23].
Prostatic inflammation represents an important factor in influencing
5302-5304
11.
12.
13.
14.
15.
16.
17.
18.
ACKNOWLEDGEMENT
Authors are thankful to Dr. Sujit Joshi, MD (Path) for carrying out histopathological study of samples.
19.
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20.
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