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Presented by
Tithi Roy 2008
Shams Bin Bony Amin 2009431004
Probal Sen - 2009431016
Md. Tanvir Ahmed - 2009431022
Monoclonal Antibodies
Monoclonal antibodies (MAb) are antibodies
produced from identical B immune cells.
Named Monoclonal because they come from single
type (clone) of B cells.
Recognizes a single and specific antigen.
Wide application in biochemistry, molecular biology
and meidicine.
Production method of
Monoclonal Antibodies
Production involves two phases:
Cell line production
MAb purification
Production method of
Monoclonal Antibodies
Cell line production (continued):
Mouse exposed to antigen.
Splenocytes are isolated.
B lymphocytes are collected and fused with
myeloma cells
The cell culture contain mixture of both fused and
unfused cells.
Production method of
Monoclonal Antibodies
Cell line production (continued):
Selection of Hybridoma on HAT medium
Quantitative assay of antibody by ELISA and Dot
Blotting.
Production method of
Monoclonal Antibodies
Production method of
Monoclonal Antibodies
MAb purification:
Cell debris and contaminants removed by
centrifugation.
Chromatography is useful for MAb separation.
Production method of
Monoclonal Antibodies
MAb purification by Immunoaffinity chromatography:
Production of Recombinant
Monoclonal Antibodies
MAb produced in mice cause:
systemic inflammatory effects
Human anti-mouse antibodies (HAMA)
Solution:
Applications of
Monoclonal Antibodies
In diagnostic procedures like RIA and ELISA
Hormone concentration analysis.
Diagnostic imaging using Radiolabeled MAbs.
In treatment of cancer: Detection of Tumor
specific antigen
In treatment of AIDS: Cell mediated destruction of
HIV infected cells guided by MAb
Advantages of
Monoclonal Antibodies
Production of identical antibodies at industrial
scale.
High specificity: reduced possibility of crossreaction
Better result in quantitative assay of proteins.
Disadvantages of
Monoclonal Antibodies
Technology is expensive.
Time consuming proces.
Presented by
Tithi Roy 2008
Shams Bin Bony Amin 2009431004
Probal Sen - 2009431016
Md. Tanvir Ahmed - 2009431022
From Nanotechnology
to Nanobiotechnology
Nanotechnology: The techniques of producing tiny
substances having few nanometers of size.
Exhibition of unique characteristics:
Higher volume to surface ratio
Strange optical properties: Gold
nanoparticles appear black
Decreased melting point:
Gold (slab size) = M.P. 1064C
Gold (2.5 nm size) = 300C
Nanobiotechnology
Use of Nanoparticles or nano-structured substances
on biological systems.
A relatively new discipline, rendering new promise.
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Applications of Nanobiotechnology
Nanodrug or nanomedicine
Targeted killing of cancer cells
Nanobots for surgery and
diagnosis
Nanogenerators