Organizacin y estructura del genoma

microbiano

Ronnie G. Gavilan
ronniegavilan@gmail.com
INS

What is the genome?

Entire genetic complement of an
organism

How many types of genomes are

there in nature?
Prokaryotic genomes
Eukaryotic Genomes
Nuclear Genomes
Mitochondrial genomes
Choloroplast genomes

Procariotas

Genome size and gene number

Amoeba dubia: 670 billion base pairs (200 times of human)

Genome and gene sizes for Eukaryotes

NGS sequencing
methods

Automated Sanger sequencing

How do you sequence a genome by NGS?

Genome data
analysis

And NOW?

Sequence quality

Assembling

Order contigs

Gene annotation

Comparative genomics

SNP detection

Phylogenetics

Metagenmica en el
estudio de la diversidad
microbiana

Conserved and hypervariable regions

in the 16S rRNA gene

Human
Microbiome

Topographical distribution of bacteria

on skin sites