Escolar Documentos
Profissional Documentos
Cultura Documentos
ISSN 0796-6687
Code Chemical Abstracts : JSOCF2
Cote INIST (CNRS France) : <27680>
Site Web: http://www.soachim.org
Institut de Recherche en Sciences de la Sant (IRSS/CNRST) 03 BP 7192 Ouagadougou 03, Burkina Faso
Abstract: Orange-fleshed sweet potato (OFSP) is a potential source of carotenoids (provitamins A), and its flour has
been used to enrich the Misola, an infant food based flour. The new biofortified formulation presented nutritional
qualities which could contribute in protein-energy recovery and improvement of the immune status of moderate
malnourished children. By coupling chromatographic and spectral methods, -carotene, -cryptoxanthin, cryptoxanthin, lutein and zeaxanthin have been identified in the extracts of Orange-fleshed sweet potato flour. The
results indicated that enriching 30% of the Misola with OFSP flour causes optimal increases in carotenoid (3271%)
and total antioxidant (109%) contents compared to the pure Misola. Total phenolic content was not significantly
affected (only 11% relative increases) by the addition of OFSP flour. M7J3 represented the best formulation
(802.565.14 kcal/ 200g) increasing the intake of energy values of about 5% and 10%, respectively, compared to
Misola and OFSP pure flours.
Key words : OFSP, antioxidants, phenolics, carotenoids, FRAP, FCR
1. Introduction
Vitamins
are
part
of
phytochemicals
micronutrients vital for all human beings. Its
deficiency limits the capacity of the body to ensure
its own defense against diseases. In developing
countries, vitamin A deficiency (VAD) remains a
public health problem where children of 6-59
months, pregnant and lactating women are the most
vulnerable groups of the population [1-3]. This
deficiency is associated to children respiratory
diseases, diarrhoea and immune deficiency, while
for pregnant it is associated to blindness [stemming
from xerophthalmia], and anemia. Sub-Saharan
Africa presents the largest number of pre-school
children suffering from night blindness and VAD[4].
In 1995 according to the Ministry of health and the
WHO/MDIS, night blindness prevalence was 1.7%
in children of 10 years old population in six
provinces of the North of Burkina Faso while the
night blindness prevalence was 1.2 to 7.9% in the
Sangui, Passor and Bam provinces [5]. In 2002 in
the department of Kaya, a study revealed that low
serum retinol prevalence was 85% and 64%,
respectively, for children from 12 to 36 months and
for their mothers[1,6].
In Burkina Faso, recent statistical data in VAD
that covering the whole country are not available.
However, studies from different administrative
districts confirmed the occurrence of VAD. To
eradicate this nutritional problem, carotenoid-rich
food consumption is one of the main approaches
recommended in many countries [7]. OFSP has
proved to be particularly promising regarding its
high
carotenoid
content
and
its
high
bioavailability[1,8].
This work follows a previous study [9] on the
identification
of
photosensitive
molecules
(carotenoids) but responsible of OFSP flour
nutritional qualities. It concerned to take advantage
of these microconstituants by strengthening the
nutritional qualities of a local infant food flour
(Misola). Then, it aims to formulate Misola-OFSP
based infant food and to determine the physicochemical properties and micronutrients in order to
promote and popularize infant food that can be used
in combating VAD in children under five
population.
2. Materials and Methods
2.1. Materials
The OFSP flour was obtained from the storage
roots of the OFSP variety called Jewel harvested in
the centre-west region of Burkina Faso, at about
A. Hema et al
beta-carotene isomers which are the -carotene, carotene, -carotene and lycopene have been
identified in extracts of flour of the variety Jewel
(Figure 1 ; Table II).
-carotene, its isomers (-carotene, -carotene,
-carotene, lycopene) and -cryptoxanthin are the
most common precursors of vitamin A carotenoids
considered in human diet [14 - 16].
On the other hand, lutein and Zeaxanthin are
oxygenated-carotenoids from the subfamily of
xanthophylls and have beneficial effects for
consumers. Indeed, they have important antioxidant
properties [17]. Lutein and zeaxanthin filter blue
light, very energetic, which assaults the
photoreceptors of the eye (indirect antioxidant
effect); What would prevent the degeneration of the
M10J0
100
0
M9J1
90
10
M8J2
80
20
M7J3
70
30
M6J4
60
40
M5J5
50
50
M4J6
40
60
M3J7
30
70
M2J8
20
80
M1J9
10
90
M0J10
0
100
m/z
[M+H]+
553.4
537.4
569.4
553.4
569.4
Formula
C40H56O
C40H56
C40H56O2
C40H56O
C40H56O2
535.4 [M-H-18] + *
551.4 [M-H-18] + *
-
Figure 1: Liquid Chromatography Mass Spectrometry and MS/MS spectra of OFSP flour extract
A. Hema et al
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2.2. Methods
2.2.1.
Determination
of
physicochemical
parameters
Physicochemical parameters of this flour were
determined using French standard V03-050,
September 1970 for proteins, International standard
ISO-659, 1998 for fats and a differential approach
used in IRSAT-DTA for total carbohydrate
contents.
2.2.2.
Determination
of
total
antioxidant, phenolic and
carotenoid
contents:
These
contents
were
determined
spectrophotometrically using a microplate in quartz
96 wells (MP96, SAFAS spectrophotometer) and
choosing standards.
Thus, total carotenoids were assessed according
to the method described by McMurry [25,26] slightly
modified. -carotene which is absorbed at 450 nm
was taken as standard. Total carotenoids contents
were obtained by reporting absorbance of extracts
on a standard curve (y = 25.56x + 0.016; R2 =
0.999) established using -carotene. Total
carotenoid contents are expressed in mg of carotene Equivalents (BCE) per gram of sample. All
measurements were carried out in triplicate.
Using Ferric Reducing Antioxidant Power
(FRAP)[27,28] method, total antioxidant contents
which reacted with FRAP reagent to give intense
blue color (=593 nm) were determined. Results
obtained from the calibration curve equation (y =
28.67x + 0.066; R2 = 0.999), are expressed in mg of
Trolox Equivalent (TE) per gram of sample.
Total phenolic contents were determined using
the Folin-Ciocalteu reagent [29]. Approximately 60
L of appropriately diluted extract was mixed to 60
L of diluted 10-fold Folin-Ciocalteu reagent. The
mixture was left at room temperature for eight
minutes to allow the complete reaction of the FolinCiocalteu reagent on oxidizable substances or
phenoxide. Then 120 L of Na2CO3 (7.5% in water)
was added to neutralize the residual reagents. The
absorbance of the extracts were measured at 765 nm
Statistical analysis
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12
TCC (mg/g)
TAC (mg/g)
TPC (mg/g)
M10J0
0.02660.0010a
0.22420.0124A
1.82180.0764a
M9J1
0.27400.0032b
0.39250.0134CD
2.08610.0112bc
M8J2
0.54460.0041c
0.43530.0043DEF
2.18420.0143bcd
d
F
M7J3
0.89680.0039
0.46900.0233
2.03090.0331bc
M6J4
1.11140.0115e
0.39670.0170CDE
2.21560.0306cd
f
EF
M5J5
1.46860.0127
0.44470.0103
2.35350.0429de
g
BCD
M4J6
1.61920.0127
0.39120.0168
2.46460.0669e
M3J7
1.71750.0226h
0.38150.0051BC
2.36690.0799de
M2J8
1.77790.0353h
0.33960.0136B
1.99890.0028ab
M1J9
2.00790.0431i
0.24810.0136A
2.29330.0549de
M0J10
2.3670.0290 j
0.3640.0103BC
2.2770.0393de
Data are expressed as means SE of triplicate experiments. Means in a column not having a common letter are different (P<0.05).
Figure 2: Changes in phytochemical contents [TCC (a), TAC (b), TPC (c)] and physicochemical parameters (d) of Misola-OFSP formulations
4. Conclusion
This study has revealed increase in five
molecules of carotenoids when the existent infant
food flour is enriched with OFSP flour.
Flour formulation containing 30% of OFSP lead to
an optimum of total antioxidant content (0.469 mg
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