Escolar Documentos
Profissional Documentos
Cultura Documentos
Next-generation probiotics
Obesity and type 2 diabetes
Ribosome Assembly
Next-generation probiotics
Obesity and type 2 diabetes
Ribosome Assembly
Cathy Robinson
Jennifer Auchtung
- Relatively Simple
- Higher throughput
- Reduces the numbers of
animals needed for
experiments
In vivo studies in
humanized microbiota mice
SHIME
ROBOGUT
Pump
Fresh
Media
15 ml
bioreactor
Waste
Media
Environmental parameters:
-
Anaerobic
37C
pH 7-7.5
Continuously stirred
~8 hr retention time
Auchtung J.M., Robinson, C.D., and Britton, R.A. (2015). Microbiome. 3:42
0.0
Bray-Curtis Dissimilarity
stress=0.167
Donor B
Donor C
Pool
(A+B+C)
Donor A
0.0
NMDS Axis 1
Community composition
assessed by sequencing V4
region of 16S rRNA gene
(Illumina)
Dissimilarity measure based
upon shared OTUs (97% ANI)
Similar results observed with
Sorenson Dissimilarity
Auchtung J.M., Robinson, C.D., and Britton, R.A. (2015). Microbiome. 3:42
Avg BC Similarity
1.0
0.8
0.6
0.4
0.2
0.0
6 9
Time in Culture
Auchtung J.M., Robinson, C.D., and Britton, R.A. (2015). Microbiome. 3:42
0.8
0.6
Days 2-7
0.4
Day-to-day variation
~10% higher than
background
0.2
0.0
1
2
3
4
5
Days between Sampling
Auchtung J.M., Robinson, C.D., and Britton, R.A. (2015). Microbiome. 3:42
Relative Abundance
1.0
Actinobacteria
Bacteroidetes
Firmicutes
Proteobacteria
Unclassified Bacteria
Verrucomicrobia
Relative proportions of
dominant phyla similar to
fecal samples
0.5
0.0
01230123 01230123456
A
B
C
Pool
Auchtung J.M., Robinson, C.D., and Britton, R.A. (2015). Microbiome. 3:42
Add
C. difficile
Treat with
clindamycin or
mock-treat
10
11 12
106
105
104
103
mock/untreated
n=10
102
101
3
4
5
Days in Culture
clinda-treated
n=7
106
105
clinda-treated
n=3
104
clinda-treated
n=9
103
mock/untreated
n=10
102
101
3
4
5
Days in Culture