Five Kingdom Classification System

Once upon a time, all living things were lumped together into two kingdoms, namely
plants and animals (at least, that's how I learned it). Animals included every living
thing that moved, ate, and grew to a certain size and stopped growing. Plants included
every living thing that did not move or eat and that continued to grow throughout life.
It became very difficult to group some living things into one or the other, so early in
the past century the two kingdoms were expanded into five kingdoms: Protista (the
single-celled eukaryotes); Fungi (fungus and related organisms); Plantae (the plants);
Animalia (the animals); Monera (the prokaryotes). Many biologists now recognize six
distinct kingdoms, dividing Monera into the Eubacteria and Archeobacteria.

All I can say is that the sytem holds true for this week, at least. It might even hold up
for a century or two. Accepted systems of classification have changed at a far faster
pace than the species have taken to evolve, that's for certain.

Kingdoms are divided into categories called phyla, each phylum is divided into
classes, each class into orders, each order into families, each family into genera, and
each genus into species. A species represents one type of organism, such as dog, tiger
shark, Ameoba proteus (the common amoeba), Homo sapiens (us), or Acer
palmatum (Japanese maple). Note that species names should be underlined or written
in italics.

Classifying larger organisms into kingdoms is usually easy, but in a microenvironment

it can be tricky. If you have had a little biology, a good exercise is to describe
individual living things, and to try to classify them as to kingdom.

Monera (includes Eubacteria and Archeobacteria)

Individuals are single-celled, may or may not move, have a cell wall, have no
chloroplasts or other organelles, and have no nucleus. Monera are usually very tiny,
although one type, namely the blue-green bacteria, look like algae. They are
filamentous and quite long, green, but have no visible structure inside the cells. No
visible feeding mechanism. They absorb nutrients through the cell wall or produce
their own by photosynthesis.

Protista
Protists are single-celled and usually move by cilia, flagella, or by amoeboid
mechanisms. There is usually no cell wall, although some forms may have a cell wall.
They have organelles including a nucleus and may have chloroplasts, so some will be
green and others won't be. They are small, although many are big enough to be
recognized in a dissecting microscope or even with a magnifying glass. Nutrients are
acquired by photosynthesis, ingestion of other organisms, or both.

Fungi
Fungi are multicellular,with a cell wall, organelles including a nucleus, but no
chloroplasts. They have no mechanisms for locomotion. Fungi range in size from
microscopic to very large ( such as mushrooms). Nutrients are acquired by absorption.
For the most part, fungi acquire nutrients from decaying material.

Plantae
Plants are multicellular and most don't move, although gametes of some plants move
using cilia or flagella. Organelles including nucleus, chloroplasts are present, and cell
walls are present. Nutrients are acquired by photosynthesis (they all require sunlight).

Animalia
Animals are multicellular, and move with the aid of cilia, flagella, or muscular organs
based on contractile proteins. They have organelles including a nucleus, but no
chloroplasts or cell walls. Animals acquire nutrients by ingestion.

A "mini-key" to the five kingdoms

Suppose you see something in freshwater that certainly appears to be living. How can
you begin to determine what it is? Here is a key (not quite perfect) that you might use
to help determine the kingdom to which it belongs.

1. Is it green or does it have green parts?

o Yes - go to 2

o No - go to 3

2. Could be a plant or a protist, or blue-green bacteria. Make sure that the green
is really part of the organism, though. An animal might have eaten something
green, for example.

o Single-celled? go to 6
 o Multicellular? Plantae. Look for cell walls, internal structure. In the
compound microscope you might be able to see chloroplasts.

3. Could be a moneran (bacteria), protist, fungus, or animal.

o Single-celled - go to 4

o Multicellular (Look for complex or branching structure, appendages) -

go to 5

4. Could be a moneran or a protist. Can you see any detail inside the cell?

o Yes - Protista. You should be able to see at least a nucleus and/or

contractile vacuole, and a definite shape. Movement should be present,
using cilia, flagella, or amoeboid motion. Cilia or flagella may be
difficult to see.

o No - Monera. Should be quite small. May be shaped like short dashes

(rods), small dots (cocci), or curved or spiral shaped. The largest them
that is commonly found in freshwater is called Spirillum volutans. It is
spiral shaped, and can be nearly a millimeter long. Except for Spirillum,
it is very difficult to see Monerans except in a compound microscope
with special lighting.

5. Animalia or Fungi. Is it moving?

o Yes - Animalia. Movement can be by cilia, flagella, or complex,

involving parts that contract. Structure should be complex. Feeding
activity may be obvious.

o No - Fungus. Should be branched, colorless filaments. May have some

kind of fruiting body (mushrooms are a fungus, don't forget). Usually
attached to some piece of decaying matter - may form a fuzzy coating on
or around an object. In water, some bacterial infections of fish and other
animals may be mistaken for a fungus.

6. Most likely Protista. If it consists of long, unbranched greenish filaments

with no apparent structure inside, it is blue-green bacteria (sometimes
mistakenly called blue-green algae), a Moneran.
Most green protists are flagellates, that is, they move rapidly with a spiralling motion.
Unless you get them to stop, you can't really see the flagella. Watch out for colonial
protists, though, such as Volvox, which forms a spinning ball of green cells. Don't be
fooled into thinking it is a plant.

Remember, the more you observe the organism, the more sure you can be. Many
living things have stages that make them resemble members of another kingdom.
www.ruf.rice.edu/~bioslabs/studies/invertebrates/kingdoms.html