Log10MW vs Distance (mm) traveled

Question 1:

Determining the molecular size for the linear plasmid:

Measurement of plasmid: 32mm.

Therefore, gaining a size of 100.4 = 2.5Kb

Question 2:
The plasmid that is larger is the relaxed plasmid as it is placed behind the Super-
coiled plasmid within the lane, indicating that it is much slower in movement. This
slow movement will result in the observation that it is much larger in comparison to
the supercoiled plasmid hence the slow movement.
Question 3:
A simple of on deciding if the linear band is from pUC18 or from a different plasmid
is by using the method within question 1, using the standard curve in over to
determine the mass or the molecular size. Knowing this, repeating the experiment
again to now where the plasmid will travel to and compare.
Question 4:
A higher percentage gel will just make the pore size smaller, hence the larger bands
of the DNA will not travel as far compared to a smaller percentage. Hence to go
about this, will be taking into account a ratio of 0.8:1.5 so the ratio of the gel is kept
within the calculations of determining the molecular size. The plasmid size within
calculation or theoretically will be the same size, but in reality, the size will be
smaller in comparison.
Question 5:
Gel red binds to DNA due to sharing a similar structure and staining process for the
ultraviolet light detection of the DNA and RNA bands.