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Copyright Hitachi High-Technologies Corporation 2002. All rights reserved. Printed in Japan. Part No. 890-9106 TF-K (FT-LT)
CONTENTS
-i-
5. MAINTENANCE AND CHECKUP............................................................................ 5-1
5.1 Error Messages and Countermeasures................ 5-1
5.2 Troubleshooting ..................................................... 5-3
5.3 Periodic Maintenance and Checkup ..................... 5-4
5.4 Gradient Mixer ........................................................ 5-5
5.4.1 Conventional Mixer ...................................... 5-5
7. SPECIFICATIONS.................................................................................................... 7-1
7.1 Model L-2100 Low-Pressure Gradient System .... 7-1
7.2 Model L-2100 High-Pressure Gradient System.... 7-3
7.3 Model L-2130 Low-Pressure Gradient System .... 7-5
7.4 Model L-2130 High-Pressure Gradient System.... 7-7
- ii -
1.1
1. OUTLINE
1.1 Gradients
1-1
2.1.1
2.1 Assembly
2.1.1 Assembly of The piping arrangement for the low-pressure gradient system is
Low-Pressure shown below.
Gradient System
WARNING
Ignition of Flammable Chemicals!
WARNING
Explosion of Vapor from Flammable Chemicals!
2-1
2.1 Assembly
WARNING
Beware of Electric Shock!
Front panel
2-2
2.1.1
Connector
Screws Tubes
2-3
2.1 Assembly
Four-way joint
2-4
2.1.1
0.33TT
0.25TT
Colum
0.8ST
Detector
1 Drain
Flow cell unit 2
6 3 0.25ST
Pump
Drain valve Sample drain
bottle
c Mixer
Drain
D
Pump head
C
B
A
a b
2-5
2.1 Assembly
3) Stabilization
Make sure that no air is introduced.
2-6
2.1.2
(1) Wiring
WARNING
Ground properly to prevent an electric shock hazard!
2-7
2.1 Assembly
(3) Tubing
WARNING
Ignition of flammable chemicals!
WARNING
Explosion of vapor from flammable chemicals!
2-8
2.1.2
WARNING
Beware of electric shock!
Screws
2-9
2.1 Assembly
Connectors Screws
2 - 10
2.1.2
2 - 11
2.1 Assembly
0.33TT
0.25TT
Column
0.8ST
Detector
1 2 Drain
Flow cell unit
6 3 0.25ST
Pump
Sample drain
Drain valve bottle
0.25ST
Mixer
Drain Pump head
0.25ST
Pump
Drain valve
1.5TT
2 - 12
3.1
3. OPERATING PROCEDURES
3-1
3.1 Creating an Analytical Program
TN TN+1 TN TN+1
3-2
3.1.1
PROG
* On the subsequent screens, press the ENT key
after input of a numeric value.
MONITOR
screen
3-3
3.1 Creating an Analytical Program
Enter 1 .
The specified existing program will be deleted.
3-4
3.1.3
3.1.3 How to Remove a Demonstrated below is how to remove a step from a program.
Step
PROG
ESC
3-5
3.1 Creating an Analytical Program
3-6
3.2.1
UTLTY
POWER ON + (SETUP)
UTLTY
Turn power on while holding down the (SETUP) key.
UTLTY
The SETUP screen will then appear. Note that the (SETUP)
key should be held down until the SETUP screen comes up.
PUMP (A = 1, B = 2)
1
3-7
3.2 Low-Pressure Gradient Mode
POWER OFF
POWER ON
3.2.2 Low-Pressure In the low-pressure gradient system, specify the kind of mixer,
Gradient Type requirement/non-requirement of degasser, type of gradient
Setting operation according to the kind of solvent and flow rate to set up
an instrumental arrangement. In general, a mixer having a
larger capacity is required as the flow rate increases.
Furthermore, it is advisable to prepare a large-capacity mixer for
mixing sparingly mixable solvents. Use a degasser to enhance
accuracy in mixing ratio for ensuring good reproducibility in
analysis. The following table shows examples of instrumental
arrangements for mixing water and methanol.
* The allowable flow rate range per flow path in the L-2100/
2130 degasser is 3.0 mL/min or less.
3-8
3.2.3
PROG
3-9
3.2 Low-Pressure Gradient Mode
3.2.4 Manual Operation Demonstrated below is a manual operation procedure in the low-
Procedure pressure gradient mode.
Use manual operation for liquid feeding under a certain condition
without using a program.
MONITOR
screen
MONITOR
screen
3 - 10
3.2.5
3.2.5 Analytical
Operation WARNING
Ignition of Flammable Chemicals!
WARNING
Explosion of Vapor from Flammable Chemicals!
3 - 11
3.2 Low-Pressure Gradient Mode
(1) Check that the pump, column, detector and data processor
are connected properly.
(2) Set the mobile phase to be used and the waste solution
bottle.
(3) Start up the pump, and wait until the flow path is filled with
the mobile phase.
(6) After completion of analysis, turn power off and treat waste
solution properly.
3 - 12
3.3.1
3.3.1 Pump Mode In the high-pressure gradient mode, set up liquid feed conditions
Setting for each of PUMP A and PUMP B.
UTLTY
POWER ON + (SETUP)
UTLTY
(SETUP)
Turn power on while holding down the key. UTLTY
The SETUP screen will then appear. Note that the (SETUP)
key should be held down until the SETUP screen comes up.
PUMP (A = 1, B = 2)
1
3 - 13
3.3 High-Pressure Gradient Mode
POWER OFF
POWER ON
UTLTY
POWER ON + (SETUP)
UTLTY
(SETUP)
Turn power on while holding down the key.
UTLTY
The SETUP screen will then appear. Note that the (SETUP)
key should be held down until the SETUP screen comes up.
3 - 14
3.3.1
PUMP (A = 1, B = 2)
2
POWER OFF
PUMP B [- -]
3 - 15
3.3 High-Pressure Gradient Mode
PROG
ESC
MONITOR
screen
3 - 16
3.3.3
MONITOR
screen
MONITOR
screen
3 - 17
3.4 Utilities
3.4 Utilities
3.4.1 Pump Motor In the high-pressure gradient mode, you can make pump motor
Control Setting control (M. CONTROL) selection.
In common practice, select STANDARD for M. CONTROL.
When the STANDARD mode is selected in which automatic flow
control is performed, pump-to-pump pressure may be affected
under a certain operating condition (high-pressure gradient,
reaction pump), causing an increase in baseline fluctuation.
In such a case, it is advisable to use the FIX mode in which
invariable flow rate control is performed for reducing the adverse
effect. Note, however, that the FIX mode may provide poor
baseline stability in an application where significant pressure
fluctuation occurs due to variation in mixing ratio. In this case, it
is recommended to select the STANDARD mode.
300
Organic solvent
200
100
Aqueous solvent
0
0 9.8 19.6 29.4 39.2
3 - 18
3.4.1
3 - 19
3.4 Utilities
UTLTY
Select CONDITION.
Press the 1 key, followed by the ENT key.
Select MOTOR-CONTROL.
Press the 2 key, followed by the ENT key.
MONITOR
screen
3 - 20
3.4.2
3.4.2 Link Program This function is available for linking two or more programs.
Function
NOTICE: For connection with Ez chrom Elite for Hitachi,
specify NO. This setting can be made on the
System Manager.
MONITOR
screen
UTLTY
(SETUP)
Select YES.
1 ENT
MONITOR
screen
3 - 21
3.4 Utilities
0 ENT
ESC
(LOCK OFF)
MONITOR
screen
Item Description
STEPS It is not permitted to input an execution step
number. Step numbers are counted successively.
Up to nine steps are allowed.
PROG - NO. Enter a code number of the program to be linked.
The allowable input range is 1 to 9.
CYCLE Enter a repeat count of the program concerned.
The allowable input range is 1 to 99.
COND. TIME Enter a conditioning time for the execution step
concerned. The allowable input range is 0.0 to
600.0 (min). During the conditioning time, liquid
feeding is performed under the initial condition
(TIME = 0.0) of the execution step. After the
lapse of the conditioning time, program execution is
started upon input of the start signal.
3 - 22
3.4.2
i. Programs
PROGRAM No. = 1
PROGRAM No. = 2
3 - 23
3.4 Utilities
3 - 24
3.4.2
Initial
TIME %A %B %C %D FLOW PRESS [IN]
0.0 10 90 0 0 1.000 5.5 1
Gradient
TIME %A %B %C %D FLOW PRESS [GR]
10.0 20 80 0 0 1.000 5.5 1
Conditioning time
TIME %A %B %C %D FLOW PRESS [LC]
0.0 30 70 0 0 1.000 5.5 2
Initial
TIME %A %B %C %D FLOW PRESS [IN]
0.0 30 70 0 0 1.000 5.5 2
Gradient
TIME %A %B %C %D FLOW PRESS [GR]
10.0 40 60 0 0 1.000 5.5 2
Conditioning time
TIME %A %B %C %D FLOW PRESS [LC]
0.0 10 90 0 0 1.000 5.5 1
3 - 25
3.4 Utilities
Column flushing
TIME %A %B %C %D FLOW PRESS [FL]
0.0 10 90 0 0 1.000 5.5 1
3 - 26
3.4.2
Initial
Gradient
TIME %A %B %C %D FLOW PRESS [GR]
10.0 20 80 0 0 1.000 5.5 1
Conditioning time
Delay time
TIME %A %B %C %D FLOW PRESS [IN]
0.0 30 70 0 0 1.000 5.5 2
Column flushing
TIME %A %B %C %D FLOW PRESS [FL]
0.0 10 90 0 0 1.000 5.5 2
3 - 27
3.4 Utilities
Initial
TIME %A %B %C %D FLOW PRESS [IN]
0.0 10 90 0 0 1.000 5.5 1
Gradient
TIME %A %B %C %D FLOW PRESS [GR]
10.0 20 80 0 0 1.000 5.5 1
Initial
TIME %A %B %C %D FLOW PRESS [LC]
0.0 10 90 0 0 1.000 5.5 2
Gradient
TIME %A %B %C %D FLOW PRESS [IN]
10.0 20 80 0 0 1.000 5.5 1
3 - 28
3.4.2
Conditioning time
TIME %A %B %C %D FLOW PRESS [LC]
0.0 30 70 0 0 1.000 5.5 2
Initial
TIME %A %B %C %D FLOW PRESS [IN]
0.0 30 70 0 0 1.000 5.5 2
Gradient
TIME %A %B %C %D FLOW PRESS [GR]
0.0 40 60 0 0 1.000 5.5 2
Conditioning time
TIME %A %B %C %D FLOW PRESS [LC]
0.0 10 90 0 0 1.000 5.5 1
Column flushing
TIME %A %B %C %D FLOW PRESS [FL]
0.0 10 90 0 0 1.000 5.5 1
3 - 29
3.4 Utilities
3.4.3 Column Flush Take the following procedure for setting up automatic stop of the
Setting pump and column flushing after completion of analysis.
Where column flushing is set up, automatic washing is
performed conveniently after completion of analysis.
For instance, when a salt solvent has been used in analysis, it is
preferable to wash the column for preventing salt deposition.
Demonstrated below is an example in which column flushing is
performed with 100% solution C for a period of 30 minutes after
a lapse of 15 minutes following the end of analysis.
MONITOR
screen
UTLTY
(SETUP)
Select FLUSH.
MONITOR
screen
1 ENT
3 - 30
3.4.3
MONITOR
screen
2 ENT MONITOR
screen
3 - 31
3.4 Utilities
End of gradient
analysis
3 - 32
3.4.3
Delay time
Stabilization time
ALL END received
from L-2200
Stabilization time
NOTICE: The pump does not stop even when the ALL END
signal is received from the L-2200.
3 - 33
3.4 Utilities
3.4.4 Event Code An event can be carried out in the manual mode. When an
Setting event code is input, the event function concerned is made active.
It is thus allowed to start an external instrument, stop the pump,
and sound the buzzer.
On occurrence of a pressure limiter error, a pressure signal
indication can be given for event No. 4.
MONITOR
screen
UTLTY
(SETUP)
MONITOR
screen
MONITOR
screen
3 - 34
3.4.4
3 - 35
4.1
4. PERFORMANCE CHECK
WARNING
Ignition of Flammable Chemicals!
WARNING
Explosion of Vapor from Flammable Chemicals!
4-1
4.1 Checking the Mixing Ratio Accuracy in a Gradient System
Under the above condition, perform air purging for at least ten
minutes (with the drain valve open).
4-2
4.1.1
(contd)
C, D
Plug
810-1348
4-3
4.1 Checking the Mixing Ratio Accuracy in a Gradient System
4-4
4.1.3
4.1.3 Mixing Ratio Calculate the mixing ratio at 10, 50 and 90% with reference to 0
Calculation to 100%.
Method
100%
90%
50%
H100
H90
H50
10%
0% H10
Mobile phase B
Start
H10
Mixing ratio: B10 = 100 (%)
H100
H50
B50 = 100 (%)
H100
H90
B90 = 100 (%)
H100
4-5
5.1
WARNING
Beware of Electric Shock in Contact with
Inside of Instrument!
5-1
5.1 Error Messages and Countermeasures
5-2
5.2
5.2 Troubleshooting
NOTE: For troubleshooting procedures concerning other modules such as the L-2200 and L-2300,
refer to the instruction manual accompanying each module.
5-3
5.3 Periodic Maintenance and Checkup
5-4
5.4.1
In the gradient system, the gradient mixer is used for mixing two
or more solvents. For low-pressure or high-pressure gradient
analysis, select the conventional mixer or semi-micro mixer
according to the flow rate to be used.
(b) Remove glass beads from the joint, and put them into
a beaker (50 to 100 mL). Then, pour a detergent
(such as a mobile phase) into the beaker.
(c) Place the filter and joint into another beaker, and pour
a detergent into it.
5-5
6.1
6. SPARE PARTS
Component Standard
Configuration/
Part No. Part Name (Recommended frequency Useful
Specifications
of replacement) Life
810-1031 Flow path filter Outlet of mixer 1920
(One year) hours
6-1
7.1
7. SPECIFICATIONS
Up to four liquids
Linear, multi-stepped
7-1
7.1 Model L-2100 Low-Pressure Gradient System
(a) Time
Up to 9 programs
7-2
7.2
Up to two liquids
Linear, multi-stepped
7-3
7.2 Model L-2100 High-Pressure Gradient System
(a) Time
Up to 9 programs
7-4
7.3
Up to four liquids
Linear, multi-stepped
9 programs
7-5
7.3 Model L-2130 Low-Pressure Gradient System
(a) Time
(f) Buzzer
7-6
7.4
Up to two liquids
Linear, multi-stepped
9 programs
7-7
7.4 Model L-2130 High-Pressure Gradient System
(a) Time
(f) Buzzer
7-8