Quarter 2, 2016-2017 Teacher: N.T.

Linh
ECH 160 Homework 1
21st Feb 2016
Due on 27th Feb 2016
Problem 1. The following data were obtained from enzymatic oxidation of phenol by oxidase at
different phenol concentrations:
S (mg/l): 10 20 30 50 60 80 90 110 130 140 150
V (mg/l.h) 5 7.5 10 12.5 13.7 15 15 12.5 9.5 7.5 5.7
a) What type of inhibition is this?
b) Determine the constants Vm, Km.
c) Determine the oxidation rate at [S] = 70 mg/l.
Problem 2. Consider the case of two enzymes immobilized on the same nonporous surface, S is a
substrate used by both enzymes in the following reactions:
S + E1 E1 + P1 (1)
S + E2 E2 + P2 (2)
The final product P3 is formed by the spontaneous reaction of P1 and P2:
P 1 + P2 P3 (3)
Reactions 1 and 2 occur only at the surface, reaction 3 is a homogenous reaction occuring throughout
the bulk liquid phase.
The following data were obtained from the reactions at different substrate concentrations:
S1 (mg/l) V1x105(mg/cm3.s) V2x105(mg/cm3.s)
25 0.37 0.20
50 0.60 0.32
75 0.75 0.70
100 0.82 0.86
125 0.90 1.20
150 0.95 1.40
175 0.97 1.61
200 0.98 1.75
225 1.00 1.90
250 1.00 1.97
275 - 2.09
300 - 2.18
325 - 2.19
350 - 2.20
375 - 2.20
400 - 2.20
500 - 2.20
a) Find Km, Vmax
b) If KL = 6x10-5 cm/s and the bulk concentration of substrate is 500 mg/l, what is the total rate of
substrate disappearance?
c) What is the overall effectiveness factor () under the conditions of part b?
d) What will be the ratio of P2 to P1 under the conditions of part b?
e) If you want to produce equimolecular amounts of P1 and P2 anf if KL = 6 x 10-5 cm/s, what value
of bulk substrate concentration must you pick?