AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

A Study of the Effect of the BioCharger NG Subtle Energy

Revitalization Platform on Overall Wellness and Heart Rate
Variability (HRV)

Study Overview
This was a small investigative study into the possible effects of the
BioCharger Subtle Energy Revitalization Platform on overall wellness
evaluations and levels of fitness as measured by Heart Rate Variability.

Fifteen men and women with an average age of 58 participated in this

study. All but two participants were older than 50 with the oldest being 78.
The majority (87%) were women. 60% were experiencing health issues prior
to using the BioCharger.

Each participant took approximately four weeks to complete the study. In

the baseline period, they were given a questionnaire evaluating their overall
wellness levels across the following metrics: sleep quality, emotional well-
being, pain levels, concentration, mental alertness and overall quality of
life.

They were also asked to do a series of challenge tests using the Nerve
Express Heart Rate Variability assessment tool. Heart Rate Variability
(HRV), a characteristic that is potentially increased by physical activity,
stress management and wellness modalities is a generalized, strong
indicator of health. A high variable heart rate is a sign of health because it
is a sign of the flexibility of the heart and of the capacity of the autonomic
nervous system to adapt to changes in demands. HRV typically decreases
with age, stress, fatigue and illness.

The HRV test was applied using a standard Polar Heart Rate chest strap and
the Nerve Express HRV analysis software program. The primary challenge
test addressed in the study is the Orthotest to evaluate physical fitness and
overall wellness. The Orthotest measures the metabolic changes as a
subject is lying down, transitions to standing up and remains standing for a
period of time. The results of the Orthotest are translated into a simple
chart (Fitnogram) that can give a reliable look into the parasympathetic and
sympathetic nervous system.

The Fitnogram chart is an instant snapshot, immediately and directly

demonstrating the fitness or functional rating of the subject. While there is
more in depth analysis of the HRV that can be undertaken, the Fitnogram is
an excellent tool to quickly review the current functional state of a subject.

Participants were evaluated six times in the baseline period without the
BioCharger. In the post control period, they used the BioCharger nine times.

1 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

They filled out the survey on a regular interval to ensure consistency and
were subject to the Orthotest before and after each BioCharger session.
Participants were instructed to continue their normal routines without
making any changes to their lifestyle during the entire study.

2 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

SURVEY RESULTS

Sleep Quality
Good quality sleep is critical to good health and well-being. Research has
shown that sleep plays a vital role in immune function, metabolism, memory,
learning and other vital functions.

After 9 sessions of using the BioCharger, participants evaluated their sleep

quality as 26% better.

Average Ratings Baseli After 9 Perce

1: Lowest -- 5: Highest ne Session nt
s Chang
e
Sleep Soundly 3.1 3.7 17%
Wake Up Feeling Refreshed 2.5 3.3 35%
Sleep Consistently Without Waking Up 2.1 2.7 29%
Overall Average 2.6 3.2 26%

Emotional Well Being

Strong emotional health allows individuals to participate in life to the fullest
extent possible through productive, meaningful activities and strong
relationships. These characteristics tend to help people deal with stress
when faced with life's challenges.

The majority of the participants felt more confident, joyous, positive and
less anxious after using the BioCharger.

Average Ratings Perce

After 9
1: Lowest -- 5: Highest Baseli nt
Session
ne Chang
s
e
Confidence 3.5 3.9 13%
Joy and Optimism (Positivity, Less 3.4 3.9 17%
Anxiety)
Calmness and Able to Deal With Stress 3.5 3.7 8%

3 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

Overall Average 3.4 3.9 13%

4 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

Concentration and Mental Alertness

The ability to concentrate and pay attention allow us to prioritize relevant
over irrelevant information that are competing for attention. Prioritization
and selection of information influences what we perceive, remember,
decide, do and remember. As technology continues to impact the amount of
stimulation and data that surround us, our ability to pay attention become
more essential to cope and work productively.

We found a 11% increase in concentration and mental alertness on average

among the participants.

Average Ratings Perce

After 9
1: Lowest -- 5: Highest Baseli nt
Session
ne Chang
s
e
Good attention span. Can focus and 3.4 3.7 10%
concentrate.
Feel mentally alert throughout the day 3.2 3.6 13%
Overall Average 3.3 3.7 11%

Pain
According to the 2012 National Health Interview Survey, 25.3 million
experience pain on a daily basis. Even moderate levels of pain can impact
productivity, daily activities and relationships.

While pain was not a significant issue with the majority of the participants,
they reported 12% less pain on average at the end of the study.

Average Ratings Perce

After 9
1: Lowest -- 5: Highest Baseli nt
Session
ne Chang
s
e
Chronic Pain 2.0 1.9 -7%
Pain/Discomfort That Interferes with 2.1 1.7 -19%
Gen. Activities
Pain/Discomfort That Impacts Mood 2.1 1.9 -9%

5 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

Overall Average 2.1 1.8 -12%

6 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

Quality of Life

93% of the participants reported at least some improvement to the quality

of life, health and well-being at the end of the study. All these participants
were interested in continuing to use the BioCharger at the completion of
the study. Many commented that they were sorry the study was ending.

Question: Has your quality of life, health

and well-being improved by using the %
BioCharger?
Noticeable Improvement 27%
Some Improvement 66%
No Improvement 7%
Deterioration 0%

Actual Participant Feedback:

I have more energy than before these sessions.

My feelings of general well-being have escalated.
I am sleeping better.
I would like to do this every day. I just feel better. My action and
reaction to myself and environment is on a more thoughtful level. I am
more grounded & confident. I feel more open and lighthearted.
I have been gradually feeling some relief from chronic hip pain.
I feel a like I am in a bit better of a mood in general.

7 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

HEART RATE VARIABILITY FITNOGRAM

There are three main zones in a Fitnogram. Blue (top fitness zone -
professional athletes), green (normal fitness zone) and red (below normal
fitness zone). The vertical axis of a Fitnogram consists of 7 Levels of
Adaptation Reserve. They represent the levels of cardiovascular adaptability
calculated on the basis of the Transition Period parameters. The adaptive
reserve component shows how much reserve energy is in the body and
available to do work. The horizontal axis of the Fitnograph represents the
Functional Capacity. The Functional Capacity represents different levels of
functioning of physiological systems. Functional Capacity is a measure of
the body's readiness and capability to mobilize the stored adaptive reserve
energy.

RESULTS

Since HRV tends to typically decrease with age, stress, fatigue or illness,
the majority of the participants scored on the lower end of the Fitnogram
during the baseline period. After nine sessions of using the BioCharger and
not changing their lifestyle or exercise regiment, 67% saw a positive change
in their Heart Rate Variability Fitnogram results. The overall change for the
group was around 5% better on average.

Adaptation Reserve: 7% Better

Functional Capacity: 4% Better

8 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

CASE STUDY
Here's a case study of a 62 year old male participant who showed promising
results in the study. The participant exercises three to five times a week on
average and did not have any ongoing ailments or health issues. However,
he has a stressful and demanding career. His work schedule is irregular as
he typically works in shifts.

Survey Results
His overall emotional well-being ratings increased 43%. Not only did he feel
significantly better, his sleep was more restful and his mental alertness
increased 20%.

Average Ratings Perce

After 9
1: Lowest -- 5: Highest Baseli nt
Session
ne Chang
s
e
Emotional Well-Being 2.8 4.0 43%
Sleep Quality 3.3 4.0 20%
Concentration and Mental Alertness 3.3 4.0 20%

Heart Rate Variability Fitness Results

The stress in his life was evident

in the baseline period with his low
Fitnogram results.
After using the BioCharger for
approximately four weeks, there
was a 31% increase in
Adaptation Reserve and 13%
increase in Functional
Capacity.

Individual baseline measures compared to post control measures

9 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |

 AUGUST 2015 BIOCHARGER AND OVERALL WELLNESS

CONCLUSION:
This modest investigative study demonstrated a positive effect of exposure
to the BioCharger NG subtle energy revitalization platform. Heart Rate
Variability demonstrated a positive effect in overall fitness and wellness in a
short period of time. In addition, survey results show improvement in sleep,
concentration/mental alertness, pain/discomfort, emotional well being, and
quality of life.

10 Advanced Biotechnologies, LLC | www.biocharger.com | (508) 694-7824 |