Titration Lab

Food science
Date: 15 November 2014

Group members:

Wanvisa Pheadphong

(Fah)

Tanaree Chatyingmongkol (Mind)

Arisara Uttagosol (Nuk)

Aimwadee Kiatsukasem (Ami)

Introduction:

Neutralization is chemical reaction which hydrogen ions from

acid react with hydroxide ions from a base to produce water

molecules and salt.

Titration is common method used in laboratory to determine

concentration if acid or base by neutralization of acid or base.

 Acid-base titration experiment is use to find concentration of

acetic acid in vinegar by performing an acid base titration in

which vinegar is neutralized by 0.1M NaOH solution.

Formulas

Calculating concentration

Molarity (Concentration) = Number of moles of solute

Volume

of solution (L)

Calculating moles

Number of moles = Mass

Molar mass

Molarity is the number of moles of solute per liter of

solution.

Molar mass is total mass of elements in 1 molecule

In this experiment we use Base (NaOH) 0.1M and Acid

(Vinegar)
 Purpose: To find concentration of acetic acid in vinegar by

performing an acid base titration.

Materials:

1. 2 burets

2. Erlenmeyer flask

3. Phenolphalein solution

4. Goggles ,Gloves ,Lab coat

5. 2 rings stands with clamps to hold

the burets

6. Base (NaOH)

7. Acid (Vinegar)

Procedure:

1. Put on lab coat, gloves, and safety goggles (to be worn

throughout the whole experiment)

2. Obtain one 50-mL buret on a ring stand. Fill the buret

with NaOH
3. Add 20-mL of vinegar from the graduated cylinder to a

clean 250-mL Erlenmeyer flask.

4. Add 3 drops of the indicator phenolphthalein to the

vinegar. Place the flask under the tip of the buret containing

NaOH.

5. Slowly add the NaOH to the vinegar. A pink color appears

where the base first contacts the acid. Swirl the flask until the

color disappears.

6. Add the base, drop by drop, while swirling after each

drop, until the base turns the solution to a pale pink that does

not disappear.

7. Record the volume of the base NaOH used.

8. Wash out the flask and repeat the titration two more times,

refilling the NaOH buret if necessary.

9. Record the volume of the base NaOH used.

10. Average the 3 volumes your group used.

Analyzing results questions:

 1. What was the average volume of NaOH used by your

group during the titration experiment? Average volume of

Acetic Acid?

Our group used average volume of NaOH is 7 mL and Acetic

Acid 20 mL during the titration experiment.

2. What is the concentration of Acetic Acid in Vinegar?

Show all formulas and your calculations.

Concentration of Acetic Acid in Vinegar is 0.035

Solution : average volume of NaOH is 7 mL

Molarity (Concentration) = Number of moles of solute

Volume

of solution (L)

0.1 M (NaOH) = Number of moles of solute

0.007

Number of moles of solute = 0.0007

Molarity (Vinegar) = 0.0007

 0.02

= 0.07

= 0.035

3. What was the function of the indicator in this

experiment? How did it work?

We add 3 drops of phenolphalein solution in 20ml of vinegar

and slowly add the NaOH to the vinegar and swirling it until

turn to a pale pink not disappears that means phenolphalein

solution is measure pH scales and show by color because at

first contact when we add NaOH a pink color appears and

when we swirl it pink color will disappear that mean it not

neutilized yet but when we add more NaOH swirl the flask

until the color disappears mean it neutilized

4. If a neutralization reaction produces salt and water, write

the chemical equation for our experiment, and the name of

the salt produced. (You may have to research this)

 HCl (aq) + NaOH (aq) NaCl (aq) +

H2O (l)

And produce salt name: Sodium acetate.

5. Critical Thinking: Soda has a very low pH (around 2 or

3).

When drinking soda you ingest carbonic acid H2Co3. Person

A drinks 1 soda each day. Person B drinks 10 sodas a

day.

Why does the pH of their blood remain the same? (around

7.35-7.45) Why does the carbonic acid not make the blood

acidic? (Research required)

Carbonic acid not make the blood acidic because after drink

soda. It will produce H2O and CO2 and when we exhale we

take CO2 out that mean acid is not remain in blood.

Conclusion: In this acid-base titration experiment we use 50

ml. burets fill with NaOH and 20-ml. of vinegar that have 3

drops of the phenolphthalein solution. Then we slowly add

the NaOH to the vinegar and swirling it until turn to a pale

pink not disappears if we add too much NaOH it will turn to

dark pink. It happen like this because when we slowly add

base to acid solution until reach point at which neutralization

occurs that called equivalence point which is number of

moles of acid equal to number of moles of base we can see

when it stays pink even mix it around. Then we record the

volume of the base NaOH that used and do two more times.

We have average of NaOH that our group used in 3 times is

7 ml and use average of NaOH to calculate concentration if

vinegar by this formula

Molarity (Concentration) = Number of moles of solute

 Volume

of solution (L)

Average volume of NaOH is 7 mL

0.1 M (NaOH) = Number of moles of solute

0.007

Number of moles of solute = 0.0007

Molarity (Vinegar) = 0.0007

0.02

= 0.035

And we can get Molarity of vinegar which is 0.035M