3.5 – Transcription and Translation

3.5.1 - Compare the structure of RNA and DNA

Stands for
Deoxyribonucleic Acid
Sugar: deoxyribose
Very long
Bases: G C T A
Double strands
wound in a helix and
held together by
hydrogen bonds
Stands for Ribonucleic
Sugar: ribose
Relatively short
Bases: G C U A
Single strand that can
form mRNA, tRNA or
3.5.2 - Outline DNA transcription in terms of the formation of an RNA strand
complementary to the DNA strand by RNA polymerase
A complimentary copy of the DNA is made in the nucleus to form the mRNA. This process is
catalysed by the enzyme RNA polymerase. To copy the mRNA, the DNA double helix is
unwound by DNA helicase, with the hydrogen bonds breaking between the base pairs to be
copied. The DNA opens at the transcription site, or position of the gene that needs to be
The coding strand, or the sense strand, is the template for the mRNA. However, the mRNA
is actually built against the anti-sense strand. It has the same pattern as the opposite strand
due to complimentary base pairing.
The free nucleotides pair with the DNA nucleotides. The only difference is that uracil
replaces thymine, bonding to adenine. The RNA polymerase forms the phosphodiester
bonds to make the backbone of the mRNA molecule. The mRNA then detaches and leaves
the nucleus via the nuclear pores in the membrane. It enters the cytoplasm for reading at
the ribosomes. The DNA double helix reforms.

3.5.3 - Describe the genetic code in terms of codons composed of triplets of bases
Each sequence of three bases codes for one amino acid, called a triplet code. These groups
of three are called codons.
For every amino acid, it has two or three triplets which code for them. Other triplets act as
the ‘start’ or ‘stop’ codons, which define where to begin and end the polypeptide sequence.
There are also multiple triplets which code for these ‘punctuation’ codons.

3.5.4 - Explain the process of translation, leading to polypeptide formation
The amino acids are activated by combining with tRNA (transfer RNA) in the cytoplasm.
tRNA molecules are in the shape of a clover leaf. Each molecule binds to a specific amino
acid codon, the other end binding to the amino acid. The other end has an anticodon, which
is the complimentary codon for the mRNA. The tRNA binds to the amino acid, catalysed by
an enzyme. This process uses ATP.

Once the mRNA molecule has been transcribed, it is sent to the ribosome in the cytoplasm
or endoplasmic reticulum for translation. The protein is formed from the polypeptides,
which are built up at the ribosomes. The ribosomes move along the mRNA the ‘read’ the
code, beginning at the start codon.
From here, the tRNA molecules, with their amino acids, find their complimentary codon on
the mRNA. The amino acids are bound in the ribosomes to form the polypeptide chains. The
tRNA then separates from the amino acid and the mRNA, and is sent back to the cytoplasm
to find more amino acids. This process continues until a stop codon is reached, at which
point the polypeptide chain is released.

In order to provide enough free amino acids for translation, heterotrophs consume them in
the protein of their diet.

The first codon on the mRNA molecule is AUG, the start codon, which bonds to the anti-
codon [UAC] on the tRNA molecule. This tRNA molecule carries the amino acids Methionine.
Codon to anti-codon binding is anti-parallel.
The polypeptides formed with fold into their shape for the protein as a result of various
intermolecular forces.

The process continues until the complete polypeptide is formed.

3.5.5 - Discuss the relationship between one gene and one polypeptide
The theory is that one gene forms one polypeptide. This is true in most cases, however
there are a few exceptions:
 Some genes code for types of RNA that do not produce polypeptides
 Some control the expression of other genes

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