CLINICAL BIOCHEMISTRY

MULTIPLE CHOICE QUESTIONS

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A QUICK RECAP OF ESSENTIAL POINTS FOR MEDICAL STUDENTS

PAPER I
 BIOCHEMISTRY
Max. Questions: 50 Basic Medical Sciences Max. Time: 45 mins
1. Which of the following contains highest amount of saturated fatty acids?

a. Ox Tallow
b. Pig Lard
c. Palm oil
d. Mustard (rapeseed) oil

2. Decarboxylation of which amino acids produces Cadaverine?

a. Ornithine
b. Tyrosine
c. Lysine
d. Leucine
e. Arginine

3. Glutathione is synthesized from

a. aspartate, cysteine, lysine

b. glutamic acid, cysteine, serine
c. glutamic acid, histidine, glycine
d. glutamic acid, cysteine, glycine
e. arginine, glycine, glutamic acid

4. Which of the following is INCORRECT about the composition of human body fat?

a. 50% oleic acid

b. 25% palmitic acid
c. 10% linoleic
d. 15% stearic acid

5. RNA involved in protein biosynthesis:

a. SnRNA
b. tRNA
c. rRNA
d. mRNA
e. miRNA

6. Which of the following RNAs is generally degraded quickly?

a. miRNA
b. rRNA
c. tRNA
d. SnRNA
e. mRNA

7. Which of the following enzymes show absolute specificity?

a. hexokinase
b. glutamate aminotransferase
c. proteases
d. glucokinase
e. urease

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8. All of the following are Pyrimidine Bases EXCEPT:

a. guanine
b. cytosine
c. thymine
d. uracil

9. Which of the following enzymes is Mitochondrial?

a. OMP decarboxylase
b. orotate phosphoribosyl transferase
c. carbamoyl phosphate synthetase-I
d. carbamoyl phosphate synthetase-II
e. Prolidase

10. All of the following are basic amino acids EXCEPT:

a. Lysine
b. Arginine
c. Leucine
d. Histidine

11. Which of the following is a serine protease inhibitor?

a. elastase
b. thrombin
c. protein C
d. alpha-1 anti-trypsin
e. cathepsin

12. Regarding the distribution of Cholesterol in human body, mark the CORRECT statement:

a. liver and spleen z 5 gm

b. muscles z40 gm
c. bone marrow z10 gm
d. blood z10 gm
e. brain z 40 gm

13. Which of the following is NOT a Glycosphingolipid?

a. Sphingomyelin
b. Cerebrosides
c. Globosides
d. Gangliosides

14. Which of the following statements regarding melting point of fatty acids is INCORRECT?

a. melting point of oleic acid is 25\C

b. melting point of stearic acid is 69\C
c. melting point of linoleic acid is -5\C
d. melting point of linolenic acid is -10\C

15. Regarding folic acid and FIGLU:

a. normally <20 mg if FIGLU is excreted in urine per day

b. FIGLU value in increased in folate deficiency
c. macrocytic anemia
d. characterized by leukopenia
e. dUMP is conversted to dTMP

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16. Which of the following is a Glycosphingolipid?

a. Sphingomyelin
b. Cerebrosides
c. Cephalin
d. Sulphated gangliosides
e. Choline plasmalogen

17. All of the following are intemediates in pyrimidine nucleotide synthesis EXCEPT:

a. uridylic acid
b. orotic acid
c. carbamoyl aspartate
d. uridine diphosphate
e. inosinic acid

18. Which of the following alters the function of mRNA?

a. miRNA
b. rRNA
c. tRNA
d. SnRNA

19. A marker enzyme for peroxisomes:

a. isomerase
b. catalase
c. phospholipase
d. peroxidase

20. Which of the following amino acids is NOT glucogenic?

a. asparagine
b. valine
c. alanine
d. histidine
e. leucine

21. Urocanic acid is derived following deamination of:

a. glutamic acid
b. FIGLU
c. histidine
d. glutamate
e. arginine

22. Which of the following statements is INCORRECT?

a. strearic acid contains no double bond

b. linolenic acids are polyunsaturated fatty acids
c. saturated fatty acids have lower melting point than unsaturated fatty acids
d. unsaturated fatty acids have lower melting point than saturated fatty acids
e. saturated fatty acids have higher melting point than unsaturated fatty acids

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23. Regarding folate deficiency, mark the INCORRECT statement:

a. dUMP is conversted to dUTP

b. thymidylate enzyme is inhibited
c. macrocytic anemia
d. characterized by leukopenia
e. dUMP is conversted to dTMP

24. Which of the following is NOT a derived lipid?

a. steroids
b. dolichols
c. leukotrienes
d. triglycerides

25. RNA involved in splicing function:

a. Small Nuclear RNAs (SnRNAs)

b. Transfer RNA (tRNA)
c. Ribosomal RNA (rRNA)
d. Messenger RNA (mRNA)
e. Micro-RNA (miRNA)

26. Which of the following apoprotein fraction specifically binds LDL receptor?

a. apo E
b. apo C-1
c. apo C-ll
d. apo B-100
e. apo A-I

27. All of the following are intermediates of pyrimidine synthesis EXCEPT:

a. uridylic acid
b. orotic acid
c. carbamoyl aspartate
d. uridine diphosphate
e. inosinic acid

28. A purely ketogenic amino acid:

a. leucine
b. tryptophan
c. valine
d. isoleucine
e. alanine

29. Which of the following fatty acids contain two (2) double bonds?

a. Linolenic acid
b. Oleic acid
c. Linoleic acid
d. Arachidonic acid

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30. Which of the following amino acids is purely Glucogenic?

a. leucine
b. tryptophan
c. tyrosine
d. serine
e. lysine

31. Glutamine is produced from

a. glutamate
b. -ketoglutarate
c. glutamate semialdehyde
d. oxaloacetate

32. Which of the following fatty acids contain NO double bond?

a. oleic acid
b. nervonic acid
c. stearic acid
d. cervonic acid

33. Which of the following inhibits LCAT?

a. apo A-lI
b. apo A-I
c. apo C-ll
d. apo C-lll
e. apo E

34. Which of the following apoprotein fraction causes activation of LCAT?

a. apo B-100
b. apo C-1
c. apo C-ll
d. apo E
e. apo A-II

35. 2, 6-di-oxopurine:

a. guanine
b. adenine
c. hypoxanthine
d. xanthine
e. uric acid

36. Which of the following enzymes is NOT involved in the pyrimidine biosynthesis?

a. orotate phosphoribosyl transferase

b. aspartyl transcarbamoylase
c. carbamoyl phosphate synthetase-I
d. carbamoyl phosphate synthetase-II
e. dihydro orotate dehydrogenase

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37. Which of the following amino acids is purely glucogenic?

a. tyrosine
b. phenyl alanine
c. isoleucine
d. histidine
e. leucine

38. Which of the following RNAs is moderately stable?

a. SnRNA
b. mRNA
c. tRNA
d. miRNA
e. rRNA

39. Orotic acid is a biological precursor of

a. Purines and pyrimidines

b. Adenylic acid and guanylic acid
c. Urea
d. Cytidylic acid and Uridylic acid

40. Which of the following best fits a 'primary transcript'?

a. rRNA
b. sRNA
c. mRNA
d. tRNA
e. SnRNA

41. An example of diamino monocarboxylic

acid:

a. leucine
b. arginine
c. valine
d. histidine
e. alanine

42. Which of the following amino acids has an imidazole ring?

a. arginine
b. tyrosine
c. asparagine
d. histidine
e. phenyl alanine

43. NOT true regarding aldosterone:

a. acts on the distal tubules

b. increases potassium ion excretion
c. controls transport of sodium ions in sweat glands
d. decreases potassium ion excretion

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44. Which of the following enzymes is NOT involved in the urea cyle?

a. argininosuccinate synthase
b. ornithine transcarbamoylase
c. carbamoyl phosphate synthetase-I
d. carbamoyl phosphate synthetase-II
e. arginase

45. Which of the following is most injurious to health?

a. Linolenic acid
b. Pentaenoic acid
c. Oleic acid
d. Elaidic acid

46. Which of the following amino acids tastes bitter?

a. Lysine
b. Isoleucine
c. Leucine
d. Serine

47. What is correct regarding transcription?

a. rRNA is involved in transcription into mRNA

b. DNA is transcribed into mRNA by tRNA
c. message is copied from the coding strand of the DNA
d. message is copied from the sense strand of the DNA

48. Which of the following amino acids contains a Indole group?

a. Proline
b. Arginine
c. Tryptophan
d. Histidine

49. All of the following amino acids are Glucogenic EXCEPT:

a. glutamine
b. leucine
c. valine
d. aspartic acid
e. cysteine

50. Which of the following amino acids contains a guanidinium group?

a. Proline
b. Arginine
c. Tryptophan
d. Tyrosine

Page 7
 BIOCHEMISTRY
Basic Medical Sciences
1 a
2 c
3 d
4 d
5 c
6 e
7 e
8 a
9 c
10 c
11 d
12 d
13 a
14 a
15 b
16 b
17 e
18 a
19 b
20 e
21 c
22 c
23 a
24 d
25 a
26 d
27 e
28 a
29 c
30 d
31 a
32 c
33 a
34 b
35 d
36 c
37 d
38 d
39 d
40 c
41 b
42 d
43 d
44 d
45 d
46 b
47 d
48 c
49 b
50 b

Page 1
PAPER II
 MEDICAL BIOCHEMISTRY
Max. Questions: 50 Pre-Clinical Sciences Max. Time: 45 mins
1. A medium potency corticosteroid:

a. Triamcinolone acetonide, 0.5%

b. Clobetasol propionate, 0.05%
c. Fluocinolone acetonide, 0.01%
d. Betamethasone valerate, 0.1%
e. Betamethasone dipropionate ointment, 0.05%

2. Which of the following alkaloid has usefulness as a sleep aid?

a. Valerian
b. Pennyroyal
c. Licorice
d. Glycyrrhizin
e. Echinacea

3. Which of the following alkaloid/herbal product has been claimed to have usefulness in major depressive disorder?

a. Valerian
b. Saint John's wort
c. Licorice
d. Chamomile
e. Pennyroyal

4. A direct precursor of l-amino butyric acid (GABA):

a. L-glutamic acid
b. Glycine
c. l-glutamylcysteinylglycine
d. Glutamine
e. N-acetyl glutamate

5. The rate limiting step in Urea synthesis

a. argininosuccinate synthetase
b. ornithine transcarbamoylase
c. carbamoyl phosphate synthetase-I
d. arginase

6. Citrulline is found in

a. CSF
b. milk
c. blood
d. tissue proteins
e. sweat

7. Which of the following is a pyridoxal phosphate (PLP) dependent enzyme?

a. Pyruvate dehydrogenase
b. Transketolase
c. Acyl-CoA synthase
d. Glutathione reductase
e. ALA synthase

8. A product which functions as a catalyst in the Urea cycle:

a. citrulline
b. ornithine
c. arginine
d. fumarate
e. urea

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9. Urea cycle disorder which is inherited as an X-linked trait:

a. Hyperammonemia type II
b. Hyperammonemia type I
c. Citrullinemia
d. Argininosuccinic aciduria
e. Hyperornithinemia

10. Which of the following enzymes is inhibited by fumarate?

a. ornithine transcarbamoylase
b. argininosuccinate synthetase
c. arginase
d. carbamoyl phosphate synthetase-II
e. argininosuccinase

11. Hyperammonemia type II is due to the deficency if enzyme:

a. Carbamoyl Phosphatase synthetase I

b. Arginase
c. Ornithine transcarbamoylase
d. Argininosuccinate synthetase
e. Argininosuccinate lyase

12. Which of the following can NOT undergo -oxidation?

a. Phytanic acid
b. Pristanic acid
c. Linoleyl-CoA
d. Dicarboxylic acids
e. Acetyl-CoA

13. Citrullinemia is due to the deficiency of enzyme:

a. Argininosuccinate synthetase
b. Arginase
c. Carbamoyl Phosphatase synthetase II
d. Argininosuccinate lyase
e. Ornithine transcarbamoylase

14. The number of amino acid residues and no. of chains in human serum albumin are

a. 972z2
b. 241z3
c. 104z1
d. 609z1
e. 574z4

15. Spot the CORRECT statement about plasma amino acids:

a. plasma amino acid level is 40-80%

b. average half life is z5 mins
c. amino acids are generally stored in the liver
d. in adult, 80% amino acids are formed from protein degradation
e. glutamine and aspartate are present in high concentrations in blood

16. The amino acids found in urine in patients with Maple syrup urine disease

a. tyrosine, tryptophan
b. cysteine, methionine
c. citrulline
d. valine, leucine
e. arginin, ornithine

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17. Which of the following is an even chain unsaturated fatty acid?

a. Propionic acid
b. Palmitoleic
c. Palmitic
d. Iso valeric acid
e. Cerebronic acid

18. All of the following are pyridoxal phosphate (PLP) dependent enzymes EXCEPT:

a. Tyrosine transaminase
b. Cystathionine-beta synthase
c. Lipoamide dehydrogenase
d. DOPA-decarboxylase
e. ALA synthase

19. The first reaction of -oxidation is catalyzed by

a. Enoyl-CoA hydratase
b. -hydroxy acyl-CoA dehydrogenase
c. -keto acyl-CoA thiolase
d. Acyl-CoA dehydrogenase

20. Which of the following enzymes catalyzes the third reaction of -oxidation?

a. Acyl-CoA dehydrogenase
b. -hydroxy acyl-CoA dehydrogenase
c. -keto acyl-CoA thiolase
d. Enoyl-CoA hydratase

21. In humans, Propionate mainly arises from

a. Dihydroxyacetone phosphate
b. m-oxidation of Phytanic acid
c. -oxidation of fatty acids
d. Acetoacetic acid
e. omega-hydroxy fatty acid

22. Which of the following is a hydroxy fatty acid?

a. Cerebronic acid
b. Palmitic
c. Palmitoleic
d. Arachidic
e. Iso valeric acid

23. All of the following are transmythylation products of methionine EXCEPT:

a. Choline
b. Ethanolamine
c. Epinephrine
d. Creatine
e. Melatonin

24. Which of the following is NOT an aliphatic polyamine?

a. putrescine
b. difluromethyl ornithine
c. spermidine
d. spermine

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25. The final stage of Glycogenolysis involves:

a. Glucose-6-phosphatase
b. Glycogen phosphorylase
c. Phosphoglucomutase
d. UDP-glucose pyrophosphorylase
e. Alpha-1,6- glucosidase

26. Which of the following is a sulphur-containing glucogenic amino acid?

a. glutamate
b. methione
c. threonine
d. tryptophan
e. histidine

27. The Glucose-Alanine Cycle is also known as:

a. Cori's cycle
b. Krebs cycle
c. Lynen cycle
d. Cahill Cycle
e. Meister cycle

28. Very high potency corticosteroid:

a. Clocortolone pivalate, 0.1%

b. Clobetasol propionate, 0.05%
c. Fluocinolone acetonide, 0.01%
d. Hydrocortisone, 1.0%
e. Triamcinolone acetonide, 0.5%

29. An amino acid that undergoes transamination to form m-keto isovaleric acid:

a. Valine
b. Isoleucine
c. Leucine
d. Alanine
e. Cysteine

30. Which of the following is hepatotoxic?

a. PUFA
b. methionine
c. orotic acid
d. choline
e. pantothenic acid

31. Following Oxidative decarboxylation, which of the following amino acids forms isovaleryl CoA?

a. Tyrosine
b. Isoleucine
c. Valine
d. Leucine
e. Lysine

32. An amino acid that is involved in the formation of esters with phosphoric acid to form phosphoproteins:

a. Valine
b. Threonine
c. Leucine
d. Glutamic acid
e. Asparagine

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33. Regarding Color reactions of amino acids, which of the following is a WRONG match?

a. Nitroprusside test z Sulfhydryl (Cysteine)

b. Xanthoproteic testz Benzene ring
c. Sakaguchi's test z Guanidinium (Arginine)
d. Ninhydrin z Peptide bonds
e. Millon's test z Phenol (Tyrosine)

34. An amino acid that undergoes transamination to form m-keto beta methylvaleric acid:

a. Valine
b. Isoleucine
c. Leucine
d. Cysteine
e. Lysine

35. Amino acids that form succinyl CoA:

a. isoleucine
b. histidine
c. serine
d. tyrosine
e. proline

36. All of the following agents may contain Anti-vitamins EXCEPT:

a. Orange peel
b. Linseed
c. Sweat cloves
d. Red cabbage
e. Yeast

37. Fatty acids are derived from

a. malonyl CoA
b. acetyl CoA
c. mevalonic acid
d. squalene
e. HMG CoA

38. Which of the following is an even chain saturated fatty acid?

a. Propionic acid
b. Palmitic acid
c. Arachidonic
d. Iso valeric acid
e. Clupanodonic acid

39. Insulin promotes all of the following EXCEPT:

a. glycolysis
b. glycogenolysis
c. lipogenesis
d. glycogenesis

40. Nosteroidal isoprenoid:

a. Cholesterol
b. Dolichol
c. Mevalonate
d. Retinol
e. m-tocopherol

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41. Which of the following enzymes requires thiamine as a coenzyme?

a. methyl malonyl-COA mutase

b. pyruvate dehydrogenase
c. glutamate decarboxylase
d. cysteine synthase
e. pyruvate carboxylase

42. Which of the following is a Lipotropic factor?

a. ethanolamine
b. biotine
c. ascorbic acid
d. choline
e. ethionine

43. Amino acids that form fumarate:

a. arginine
b. tyrosine
c. methionine
d. asparagine
e. valine

44. 'Prenylation' is a term most commonly associated with:

a. amino acid biosysnthesis

b. glycogenesis
c. cholesterol biosynthesis
d. amino acid catabolism
e. beta-oxidation of fatty acids

45. A substrate analog of HMG CoA reductase:

a. miglitol
b. simvastatin
c. anastrozole
d. echinocanadin B
e. sunitinib

46. Which of the following is an inhibitor of polyamine biosynthesis?

a. Azaserine
b. Phentolamine
c. Samorine
d. Difluromethyl ornithine
e. Sanguinarine

47. The rate limiting reaction in bile acid synthesis is catalyzed by:

a. hydroxymethylglutaryl (HMG) CoA reductase

b. acetyl CoA carboxylase
c. aspartyl transcarbamoylase
d. cholesterol-7-m-hydroxylase

48. All of the following are intermediate products of amino acid catabolism EXCEPT:

a. a-ketoglutarate
b. malate
c. fumarate
d. pyruvate
e. acetyl CoA

Page 6
49. Amino acids that form m-ketoglutarate:

a. proline
b. tyrosine
c. serine
d. cystine
e. threonine

50. Degradation of phenylalanine produces:

a. acetoacetate
b. pyruvate
c. a-ketoglutarate
d. glutamate
e. methionine

Page 7
 MEDICAL BIOCHEMISTRY
Pre-Clinical Sciences
1 d
2 a
3 b
4 a
5 c
6 b
7 e
8 b
9 a
10 e
11 c
12 a
13 d
14 d
15 b
16 d
17 b
18 c
19 d
20 b
21 c
22 a
23 b
24 b
25 a
26 b
27 d
28 b
29 a
30 c
31 d
32 b
33 d
34 b
35 a
36 e
37 b
38 b
39 b
40 b
41 b
42 d
43 b
44 c
45 b
46 d
47 d
48 b
49 a
50 a

Page 1
NOTES