Application:

1. Trace sample analysis

2. PCR - Karyotyping
3. Forensic examination
4. High - specificity PCR
5. High - throughput PCR

Note:
Description: For whole blood or tissues direct PCR, we
2X Enduro PreMix is a premixed ready-to-use recommended the reaction mix should keep on ice
solution containing reaction buffer, dNTPs, loading before PCR amplification started because crude
dye, glycerol, PCR enhancers and Hot Start Taq DNA sample can result in false negative results from PCR.
polymerase specially engineered by TEN GIGA BIO
(Enduro Taq) as 2-fold concentration. Comparing Reaction Mixture for Recommended PCR assay:
with conventional Taq polymerase, Enduro Taq
prevents non-specific amplification due to Component Amount per reaction
mispriming and/or formation of primer dimers Raw material X
before thermal cycling, and it also posses 2X faster 10 M forward Primer 0.5 l
elongation rate than conventional Taq. The
10 M reverse Primer 0.5 l
polymerase activity is restored during the initial
2X Enduro PreMix 12.5 l
denaturation step when amplification reactions are
Nuclease-free water to 25 l
heated at 94-95oC for ten minutes. In addition, 2X
Total volume 25 l
Enduro PreMix is suitable for PCR amplification
directly from whole blood, and samples from cell
Thermocycling Conditions for a Routine PCR:
lines or tissues. You may skip any prior DNA
purification steps, only applying a tiny amount of
Segment Number of Step Temperature Time
raw material directly into the PCR reaction, running
cycles
in a PCR machine, and directly loading into an
1 1 Initial 95oC 10
agarose gel for electrophoresis. 2X Enduro PreMix is
denaturation minutes
a powerful tool for DNA analysis because of its
specificity and quickly. 2 35-40 Denaturation 95oC 30
seconds

Instruction of using whole blood as templates: Annealing Primer Tm 30

Heparin, EDTA or citrate treated whole blood + 3-5OC seconds

sample is all suitable for Enduro PreMix. DNA Extension 72oC 15-30
amplification can be achieved in the present of 1 ~ sec/kb
10% blood. 3 1 Final 72oC 5-10
Extension minutes
Instruction of using plant tissues as templates: Hold 14oC --
By using puncher or other tools, plant tissue with 1
mm in diameter is able to add directly into a 25 L
PCR reaction without pre-treatment. The
recommended size of plant tissue is < 1 mm in
diameter.