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Tyler Bennett

Skeletal Muscle Lab Report

This lab was completed to demonstrate the change in the muscle that happens as the
ATP enters into the muscle. This protein, including others, causes the muscles to contract and
relax, moving the body and bones. In this lab, there were many different problems that were
apparent in the lab. Some of the strands that were measured in the lab were too large. This
made it hard because when zooming in or out, it was very easy to lose your place on the strand.

The muscle relies heavily on calcium to cause contractions within the muscle. The
muscle is made up of many different organelles and bodies that help the muscle contract and
relax. The main organelle is the sarcomere, that reacts with myosin and actin to cause the
contraction. Once the sarcoplasmic reticulum releases calcium ions, the work to bind with
troponin, which causes tropomyosin to face the actin which then binds with myosin to cause
contraction. This happens in figure 1b, after the muscle become wider then figure 1a. The table
1 represents this data, because as the muscle becomes contracted, it becomes shorter.
Relaxation comes from the opposite of the contraction reaction. The calcium ions that originally
was released to bind to the troponin, is released and pumped back into the sarcoplasmic
reticulum. This causes a break in the link between actin and myosin, causing the muscle to go
back to it dormant state of relaxation. This is apparent in figure 1a, because the muscles are
Figure 1a - Muscle Strand Before ATP
These strains represent muscle before the addition of ATP. The ATP causes the muscle
to contract, making them wider, but shorter in appearance. These are relaxed and straight so
they can smoothly slide over each other. There are multiple strands of muscle fiber.

Figure 1b - Muscle Strand After ATP

This muscle represents a muscle that has be contracted with the application of APH. As
you can see, the muscles have expanded, becoming wider. This means that the muscles have
slide over each other and now rest on top of eachother. This is what causes the expansion of
the muscle.
Table 1 - Length and Width Data
Variable Trials Before ATP After ATP Initial

Length Trial 1 120mm 80mm -40mm

Trial 2 110mm 60mm -50mm

Trial 3 80mm 56mm -24mm

Width Trial 1 13mm 15mm 2mm

Trial 2 12mm 18mm 6mm

Trial 3 10mm 14mm 4mm

It is clear that the ATP does cause some type of change on the muscle. The data
represents that as the ATP is introduced, the length gets shorter, while the width gets larger.
Figure 2