GeneArt Cloning Vectors

SfiI
AscI
SacI
HindIII
Resistance XhoI
NotI
BamHI
pMX EcoRI
Vector ID
Vector Resistance ID Origin HindIII
pMA Amp GCGGAA colE1 XhoI
pMC Cmp TGCGAA colE1 NotI
pMK Kan AAGGAA colE1 BamHI
pMS Spc AGTGAA colE1 EcoRI
pMZ Zeo TACGAA colE1 KpnI
Origin
PacI
SfiI

M13 -20 primer binding site

TTGTAAAACGACGGCCAGTGAGCGCGACGTAATACGACTCAC...

ID SfiI AscI SacI HindIII XhoI NotI

...TGNNNNNNGGCCGTCAAGGCCTAGGCGCGCCATGAGCTCTAAAGCTTCGCTCGAGCTGCGGCCGC

BamHI EcoRI HindIII XhoI NotI BamHI EcoRI KpnI

ACGGATCCTCGAATTCC... ...AAGCTTATCTCGAGTAGCGGCCGCTTGGATCCCAGAATTCTAGGTACCT

PacI SfiI ID M13 -R primer binding site

CTTAATTAACTGGCCTCATGGGCCNNNNNNTC...ACATGGTCATAGCTGTTTCC

The pMX vector series is based on pUC-like cloning vectors, but leaves out unnecessary promoters (biosafety level 1).

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