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For the detection and enumeration of lipolytic microorganisms in food and other material (staphylococci,
pseudomonads, clostridiae and marine flavobacteria).
Composition:
Ingredients Grams/Litre
Special peptone 5.0
Yeast extract 3.0
Agar 12.0
Final pH 7.5 +/- 0.2 at 37C
Store prepared media below 8C, protected from direct light. Store dehydrated powder, in a dry place, in tightly-sealed
containers at 2-25C.
Directions:
Dissolve 20 g in 1 litre distilled water. Sterilize by autoclaving at 121C for 15 minutes. Let cool to 80C and add 10g
neutral Tributyrin (91010). Mix thoroughly to emulsify the Tributyrin completely. Pour plates in order to maintain
uniform turbidity. If the emulsion separates, the effectiveness of the culture medium is affected.
References:
1. J.A. Anderson, Ber. Iird. Int. Mikrobiol. Kongress, 3, 726-728 (1939)
2. A.G. Imnes, J. Appl. Bact., 19, 39-45 (1956)
3. A.T. Willis, J. Path. Bact., 80, 379-390 (1960)
4. P.R. Hayes, J. Gen. Microbiol., 30, 1-19 (1963)
5. G.M. El Sadek, T. Richards, J. Appl. Bact., 20, 137 (1959)
6. M. Rapp, Milchwirtsch., 33, 493-496 (1978)
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