Media/Test Reagent Selects For Differentiates Visible results

Brilliant green (S&S)

Salmonella and
Shigella
Pathogenic, enteric
Lactose (+) pink
bacilli
Producing H2S black
centers

SS Media

bile salts Gram (-) only

Sodium citrate Gram (-), coliforms

Lactose fermenting
MacConkey Agar
Dysentery, typhoid,
paratyphoid bacteria
Bile salts Gram (-) only

Neutral red (red if

Lactose (+) red
acidic, yellow if
Lactose (-) colorless
basic)

Mannitol Salt Agar Salt concentration

Gram (+), Staphylocci
(MSA) (7.5%)
Ferment mannitol
white (yellow media)
Phenol red
Cant colorless (pink
media)
 Except Microccus luteus yellow colonies

Streptococci,
Mitis Salivarius Agar
Enterococci

Baird Parker Medium

Tellurite Gram (+) only
(BPM)
Promote coagulase (+)
Glycine
S. aureus

LiCl Gram (+) only

Blood Agar Plate Hemolytic bacteria

partial lysis,
greenish brown
blood
full lysis, yellow
no lysis, invisble

Lactose (+) metallic

Eosin Methylene Blue Eosin methylene Gram (-) only
green sheen
 Neisseria + others
Chocolate Medium
[enriched growth]

Thayer-Martin Medium Neisseria only

Sulfur Indole Motility H2S producing black

(SIM) Test Indole red/pink layer

Semi-solid agar For motility

For detection of gas

Fermentation Studies Durham Tubes
production
 For production of H2O2
Catalase Test Streptococcus (-)
Staphylococcus (+)

Test for Cytochrome

oxidase
Coagulase Test
Antibiotic Disk Assay
Enzyme Assay
Spirit Blue Agar
Urease Test