Curso: Turma: Professor: Tarcsio Carvalho

Disciplina: Biologia Molecular Data:

Aluno (a):

Atividade Biologia Molecular

Utilizar este exemplo como modelo para apresentao da atividade

1 Utilizar letra do tipo COURRIER NEW TAMANHO 10
2 Resultados apresentados conforme abaixo
I NOME DO GENE
II SEQUNCIA DE AMINOCIDOS
III SEQUNCIA DE CDONS
IV SEQUNCIA COMPLETA DO GENE
V RESULTADO FINAL.
3 Identificar uma mutao com troca de aminocidos
4 Marcar a mudana da cadeia de aminocidos, na cadeia de cdons e na cadeia completa
do gene.
5 Proceder a criao de primers com o programa PRIMER3, fornecendo ao mesmo uma
sequncia com, no mnimo, 200 nucleotdeos antes da mutao e 200 aps a mutao.
6 Fazer uma marcao com chave alguns nucleotdeos antes e aps a mutao
Exemplo - CTGCTGCCGCT[GCCGCTGCTGTTGCTGCTGCCGCC]GCAGCCCGCCCTGG
7 Copiar o resultado do programa Primer 3
8 Identificar os primers na sequncia completa

I GENE ACE

II SEQUENCIA DE AMINOCIDOS

1 mgaasgrrgp glllplplll llppqpalal dpglqpgnfs adeagaqlfa qsynssaeqv

61 lfqsvaaswa hdtnitaena rrqeeaalls qefaeawgqk akelyepiwq nftdpqlrri
121 igavrtlgsa nlplakrqqy nallsnmsri ystakvclpn ktatcwsldp dltnilassr
181 syamllfawe gwhnaagipl kplyedftal sneaykqdgf tdtgaywrsw ynsptfeddl
241 ehlyqqlepl ylnlhafvrr alhrrygdry inlrgpipah llgdmwaqsw eniydmvvpf
301 pdkpnldvts tmlqqgwnat hmfrvaeeff tslelspmpp efwegsmlek padgrevvch
361 asawdfynrk dfrikqctrv tmdqlstvhh emghiqyylq ykdlpvslrr ganpgfheai
421 gdvlalsvst pehlhkigll drvtndtesd inyllkmale kiaflpfgyl vdqwrwgvfs
481 grtppsrynf dwwylrtkyq gicppvtrne thfdagakfh vpnvtpyiry fvsfvlqfqf
541 healckeagy egplhqcdiy rstkagaklr kvlqagssrp wqevlkdmvg ldaldaqpll
601 kyfqpvtqwl qeqnqqngev lgwpeyqwhp plpdnypegi dlvtdeaeas kfveeydrts
661 qvvwneyaea nwnyntnitt etskillqkn mqianhtlky gtqarkfdvn qlqnttikri
721 ikkvqdlera alpaqeleey nkilldmett ysvatvchpn gsclqlepdl tnvmatsrky
781 edllwawegw rdkagrailq fypkyvelin qaarlngyvd agdswrsmye tpsleqdler
841 lfqelqplyl nlhayvrral hrhygaqhin legpipahll gnmwaqtwsn iydlvvpfps
901 apsmdtteam lkqgwtprrm fkeaddffts lgllpvppef wnksmlekpt dgrevvchas
961 awdfyngkdf rikqcttvnl edlvvahhem ghiqyfmqyk dlpvalrega npgfheaigd
1021 vlalsvstpk hlhslnllss eggsdehdin flmkmaldki afipfsylvd qwrwrvfdgs
1081 itkenynqew wslrlkyqgl cppvprtqgd fdpgakfhip ssvpyiryfv sfiiqfqfhe
1141 alcqaaghtg plhkcdiyqs keagqrlata mklgfsrpwp eamqlitgqp nmsasamlsy
1201 fkplldwlrt enelhgeklg wpqynwtpns arsegplpds grvsflgldl daqqarvgqw
1261 lllflgiall vatlglsqrl fsirhrslhr hshgpqfgse velrhs

III SEQUENCIA DE CDONS DO GENE

1 ATG GGG GCC GCC TCG GGC CGC CGG GGG CCG GGG CTG CTG CTG CCG 15
16 CTG CCG CTG CTG TTG CTG CTG CCG CCG CAG CCC GCC CTG GCG TTG 30
31 GAC CCC GGG CTG CAG CCC GGC AAC TTT TCT GCT GAC GAG GCC GGG 45
46 GCG CAG CTC TTC GCG CAG AGC TAC AAC TCC AGC GCC GAA CAG GTG 60
61 CTG TTC CAG AGC GTG GCC GCC AGC TGG GCG CAC GAC ACC AAC ATC 75
76 ACC GCG GAG AAT GCA AGG CGC CAG GAG GAA GCA GCC CTG CTC AGC 90
91 CAG GAG TTT GCG GAG GCC TGG GGC CAG AAG GCC AAG GAG CTG TAT 105
106 GAA CCG ATC TGG CAG AAC TTC ACG GAC CCG CAG CTG CGC AGG ATC 120
121 ATC GGA GCT GTG CGA ACC CTG GGC TCT GCC AAC CTG CCC CTG GCT 135
136 AAG CGG CAG CAG TAC AAC GCC CTG CTA AGC AAC ATG AGC AGG ATC 150
151 TAC TCC ACC GCC AAG GTC TGC CTC CCC AAC AAG ACT GCC ACC TGC 165
166 TGG TCC CTG GAC CCA GAT CTC ACC AAC ATC CTG GCT TCC TCG CGA 180
181 AGC TAC GCC ATG CTC CTG TTT GCC TGG GAG GGC TGG CAC AAC GCT 195
196 GCG GGC ATC CCG CTG AAA CCG CTG TAC GAG GAT TTC ACT GCC CTC 210
211 AGC AAT GAA GCC TAC AAG CAG GAC GGC TTC ACA GAC ACG GGG GCC 225
226 TAC TGG CGC TCC TGG TAC AAC TCC CCC ACC TTC GAG GAC GAT CTG 240
241 GAA CAC CTC TAC CAA CAG CTA GAG CCC CTC TAC CTG AAC CTC CAT 255
256 GCC TTC GTC CGC CGC GCA CTG CAT CGC CGA TAC GGA GAC AGA TAC 270
271 ATC AAC CTC AGG GGA CCC ATC CCT GCT CAT CTG CTG GGA GAC ATG 285
286 TGG GCC CAG AGC TGG GAA AAC ATC TAC GAC ATG GTG GTG CCT TTC 300
301 CCA GAC AAG CCC AAC CTC GAT GTC ACC AGT ACT ATG CTG CAG CAG 315
316 GGC TGG AAC GCC ACG CAC ATG TTC CGG GTG GCA GAG GAG TTC TTC 330
331 ACC TCC CTG GAG CTC TCC CCC ATG CCT CCC GAG TTC TGG GAA GGG 345
346 TCG ATG CTG GAG AAG CCG GCC GAC GGG CGG GAA GTG GTG TGC CAC 360
361 GCC TCG GCT TGG GAC TTC TAC AAC AGG AAA GAC TTC AGG ATC AAG 375
376 CAG TGC ACA CGG GTC ACG ATG GAC CAG CTC TCC ACA GTG CAC CAT 390
391 GAG ATG GGC CAT ATA CAG TAC TAC CTG CAG TAC AAG GAT CTG CCC 405
406 GTC TCC CTG CGT CGG GGG GCC AAC CCC GGC TTC CAT GAG GCC ATT 420
421 GGG GAC GTG CTG GCG CTC TCG GTC TCC ACT CCT GAA CAT CTG CAC 435
436 AAA ATC GGC CTG CTG GAC CGT GTC ACC AAT GAC ACG GAA AGT GAC 450
451 ATC AAT TAC TTG CTA AAA ATG GCA CTG GAA AAA ATT GCC TTC CTG 465
466 CCC TTT GGC TAC TTG GTG GAC CAG TGG CGC TGG GGG GTC TTT AGT 480
481 GGG CGT ACC CCC CCT TCC CGC TAC AAC TTC GAC TGG TGG TAT CTT 495
496 CGA ACC AAG TAT CAG GGG ATC TGT CCT CCT GTT ACC CGA AAC GAA 510
511 ACC CAC TTT GAT GCT GGA GCT AAG TTT CAT GTT CCA AAT GTG ACA 525
526 CCA TAC ATC AGG TAC TTT GTG AGT TTT GTC CTG CAG TTC CAG TTC 540
541 CAT GAA GCC CTG TGC AAG GAG GCA GGC TAT GAG GGC CCA CTG CAC 555
556 CAG TGT GAC ATC TAC CGG TCC ACC AAG GCA GGG GCC AAG CTC CGG 570
571 AAG GTG CTG CAG GCT GGC TCC TCC AGG CCC TGG CAG GAG GTG CTG 585
586 AAG GAC ATG GTC GGC TTA GAT GCC CTG GAT GCC CAG CCG CTG CTC 600
601 AAG TAC TTC CAG CCA GTC ACC CAG TGG CTG CAG GAG CAG AAC CAG 615
616 CAG AAC GGC GAG GTC CTG GGC TGG CCC GAG TAC CAG TGG CAC CCG 630
631 CCG TTG CCT GAC AAC TAC CCG GAG GGC ATA GAC CTG GTG ACT GAT 645
646 GAG GCT GAG GCC AGC AAG TTT GTG GAG GAA TAT GAC CGG ACA TCC 660
661 CAG GTG GTG TGG AAC GAG TAT GCC GAG GCC AAC TGG AAC TAC AAC 675
676 ACC AAC ATC ACC ACA GAG ACC AGC AAG ATT CTG CTG CAG AAG AAC 690
691 ATG CAA ATA GCC AAC CAC ACC CTG AAG TAC GGC ACC CAG GCC AGG 705
706 AAG TTT GAT GTG AAC CAG TTG CAG AAC ACC ACT ATC AAG CGG ATC 720
721 ATA AAG AAG GTT CAG GAC CTA GAA CGG GCA GCG CTG CCT GCC CAG 735
736 GAG CTG GAG GAG TAC AAC AAG ATC CTG TTG GAT ATG GAA ACC ACC 750
751 TAC AGC GTG GCC ACT GTG TGC CAC CCG AAT GGC AGC TGC CTG CAG 765
766 CTC GAG CCA GAT CTG ACG AAT GTG ATG GCC ACA TCC CGG AAA TAT 780
781 GAA GAC CTG TTA TGG GCA TGG GAG GGC TGG CGA GAC AAG GCG GGG 795
796 AGA GCC ATC CTC CAG TTT TAC CCG AAA TAC GTG GAA CTC ATC AAC 810
811 CAG GCT GCC CGG CTC AAT GGC TAT GTA GAT GCA GGG GAC TCG TGG 825
826 AGG TCT ATG TAC GAG ACA CCA TCC CTG GAG CAA GAC CTG GAG CGG 840
841 CTC TTC CAG GAG CTG CAG CCA CTC TAC CTC AAC CTG CAT GCC TAC 855
856 GTG CGC CGG GCC CTG CAC CGT CAC TAC GGG GCC CAG CAC ATC AAC 870
871 CTG GAG GGG CCC ATT CCT GCT CAC CTG CTG GGG AAC ATG TGG GCG 885
886 CAG ACC TGG TCC AAC ATC TAT GAC TTG GTG GTG CCC TTC CCT TCA 900
901 GCC CCC TCG ATG GAC ACC ACA GAG GCT ATG CTA AAG CAG GGC TGG 915
916 ACG CCC AGG AGG ATG TTT AAG GAG GCT GAT GAT TTC TTC ACC TCC 930
931 CTG GGG CTG CTG CCC GTG CCT CCT GAG TTC TGG AAC AAG TCG ATG 945
946 CTG GAG AAG CCA ACC GAC GGG CGG GAG GTG GTC TGC CAC GCC TCG 960
961 GCC TGG GAC TTC TAC AAC GGC AAG GAC TTC CGG ATC AAG CAG TGC 975
976 ACC ACC GTG AAC TTG GAG GAC CTG GTG GTG GCC CAC CAC GAA ATG 990
991 GGC CAC ATC CAG TAT TTC ATG CAG TAC AAA GAC TTA CCT GTG GCC 1005
1006 TTG AGG GAG GGT GCC AAC CCC GGC TTC CAT GAG GCC ATT GGG GAC 1020
1021 GTG CTA GCC CTC TCA GTG TCT ACG CCC AAG CAC CTG CAC AGT CTC 1035
1036 AAC CTG CTG AGC AGT GAG GGT GGC AGC GAC GAG CAT GAC ATC AAC 1050
1051 TTT CTG ATG AAG ATG GCC CTT GAC AAG ATC GCC TTT ATC CCC TTC 1065
1066 AGC TAC CTC GTC GAT CAG TGG CGC TGG AGG GTA TTT GAT GGA AGC 1080
1081 ATC ACC AAG GAG AAC TAT AAC CAG GAG TGG TGG AGC CTC AGG CTG 1095
1096 AAG TAC CAG GGC CTC TGC CCC CCA GTG CCC AGG ACT CAA GGT GAC 1110
1111 TTT GAC CCA GGG GCC AAG TTC CAC ATT CCT TCT AGC GTG CCT TAC 1125
1126 ATC AGG TAC TTT GTC AGC TTC ATC ATC CAG TTC CAG TTC CAC GAG 1140
1141 GCA CTG TGC CAG GCA GCT GGC CAC ACG GGC CCC CTG CAC AAG TGT 1155
1156 GAC ATC TAC CAG TCC AAG GAG GCC GGG CAG CGC CTG GCG ACC GCC 1170
1171 ATG AAG CTG GGC TTC AGT AGG CCG TGG CCG GAA GCC ATG CAG CTG 1185
1186 ATC ACG GGC CAG CCC AAC ATG AGC GCC TCG GCC ATG TTG AGC TAC 1200
1201 TTC AAG CCG CTG CTG GAC TGG CTC CGC ACG GAG AAC GAG CTG CAT 1215
1216 GGG GAG AAG CTG GGC TGG CCG CAG TAC AAC TGG ACG CCG AAC TCC 1230
1231 GCT CGC TCA GAA GGG CCC CTC CCA GAC AGC GGC CGC GTC AGC TTC 1245
1246 CTG GGC CTG GAC CTG GAT GCG CAG CAG GCC CGC GTG GGC CAG TGG 1260
1261 CTG CTG CTC TTC CTG GGC ATC GCC CTG CTG GTA GCC ACC CTG GGC 1275
1276 CTC AGC CAG CGG CTC TTC AGC ATC CGC CAC CGC AGC CTC CAC CGG 1290
1291 CAC TCC CAC GGG CCC CAG TTC GGC TCC GAG GTG GAG CTG AGA CAC 1305
1306 TCC TGA

IV SEQUENCIA COMPLETA DO GENE

TGGCTCCCCCTCCCTCGCAGCCCCAAGCCCCAGGTGTGCCGGCCGCCCTGAGCCCCTCCAGCACCTCCCGGAGGCGCCTGCAAGACA
CCTAAGGTCCCCGCCTCCCTCCTCTCCCCCCCGCCACACCCCTACCCCCGGCAGGCGACGTCCCCGCCCCTCGACCATGGCCTGGTG
AAGAAGCCGGCCAGGCCCGATCAGCCCCATCCCCGCCGCACGAGCGGCGCCTGCGGACAGCTCCTGGGGCCCCGGCCTTGTC ACTCC
GGAGGCGGGAGGCTCCGGGGGGTCGGGCTGGGAAGATCGAGCCGGAGGCCGCTAGGCTCCCAGGCCCCGGCCGAGGCTGCGCGGCCG
CACGGTGGGCAGGCTCGGGTGTTCCGGCAAACTGCCGGGTCCCCATCTTCAAAAGAGAGGAGGCCCTTTCTCCAGCTTCCTCTGCGG Commented [U1]: -239 A/T
GAGCCCGACCCAGCCCCATCCCGCCACCCCCGGGCTGCACCTCGGCCCCTCCCCGGCCCGCGCCCCTGCCCGGGGCGGGCCAGGAAC
CTCGGCCCGCGCCGCTGGGGACTTTGGAGCGGAGGAGGAAGCGCGGCGGGGCGGGGGCGGGGGTGTGTCGGGTTTTATAACCCGCAG
GGCGGCCGCGGCGCAGGAGAAGGGGCAGAGCCGAGCACCGCGCACCGCGTCATGGGGGCCGCCTCGGGCCGCCGGGGGCCGGGGCTG
CTGCTGCCGCTGCCGCTGCTGTTGCTGCTGCCGCCGCAGCCCGCCCTGGCGTTGGACCCCGGGCTGCAGCCCGGCAACTTTTCTGCT
GACGAGGCCGGGGCGCAGCTCTTCGCGCAGAGCTACAACTCCAGCGCCGAACAGGTGCTGTTCCAGAGCGTGGCCGCCAGCTGGGCG
CACGACACCAACATCACCGCGGAGAATGCAAGGCCAGGTGGCGCCCGGGCCCGGGCGGGGGCGGGGCGGGCCGCGGCGGCCAATCAC
AGCACGCGGCCGGCTTGTGGGGCGGGCAGGCTGGCGCCCCCGACCCGAACCCCACCCCGACCCCGGACCCTCGCCCCGACAGTCAGC
CGCGGGGCCCGAGCGCCGGGCTGCGCGCACGGCCTACGCTCCCAGCATGCACGAGTTGGATGGATGAGGGTGGCTGCTCCCAGGCCG
CGCCCGCCTTCGCCGAAGGTGCTGGGCTTGGCTCTGGGGCCCCCGCGCTCTCGGGCAGCTGCCTTCTCACCTCCGGACGCTGTCGCT
GTCACCGTCACCGCACTGCACTGTCCATCCACCCTCCACTCGCCCGGCCTCTTTTCTGGTCCCAATTTCTGCTCCACCATTCCCATG
AGGCAGATTCCCTCCAGAAGGAGGAAGCGCGGCTTCCGCAAACTAAGGTCTCCCGCAGGGATCTCCCCAGGGCCCGGGCTCTGGAAG
CCCTTGGCCTTCCTCCCCTCCCCCAGCACCGTGGCTTCTCCTTTATGGCCTGCATCTAAGCAGGGTCCTACACCCTCCCTGCCCTCC
TGGTGCCCAATAGGAGGAAGCAGCCCTGCTCAGCCAGGAGTTTGCGGAGGCCTGGGGCCAGAAGGCCAAGGAGCTGTATGAACCGAT
CTGGCAGAACTTCACGGACCCGCAGCTGCGCAGGATCATCGGAGCTGTGCGCACCCTGGGCTCTGCCAACCTGCCCCTGGCTAAGCG
GCAGCAGGTGGGCTGAGGGCTGAGGCAGAGCTCGGGGGCGGCCTCCTAGTGCCCCATCGTGGGGGTCGGGGGAGAGCAGCCCATCAG
GGAGGGAGGAACCCTGGGATCCACATGGGCCCTGACAGAAGGGAAAGCCCAGGTAAGCACAGAATGGCTTTCTGAGCATTGATTTTT
CTTGGAGATGGGGTGGGGAGTTACTTTCTGTTAAAGGAAGCATTCTGGAGTAGGAAGCCAAATTCAAATACACTTCTCCCTAGGCTG
GTTTATGAGCTTCTTTGGAAGAGTTGAGAAGGGCTGGGCGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGG
CGCATCGCTTGAGCCCAGGAGTTCAAGACCAGCCTGGCCAACATGGCAAAACCTCGTCTCTACAAAAAAAAAATAGCTGGGCTTGGT
GGTGCGTGCACCTACAGTCCCAGCTACTCTTGAAACTGAGGGGGAAGGATCACCTGAGCCCAGGAGGTCAAGGCTACAGTGAGC TGT
GATTGCACTACTGCACCCCAGCCTGCGTGACAGAGTGAGACCTCCCCCCAAAAAAAAGAGAGAGAGAAAAGGTTGAGAAAGACTGGG
AAGTCACCAAAGCCAGAGAATGGGAGGGATCTGCCCTCACTGCAGGGTGGTGCCAAGCTGGGACTTGACCCTGACCCTGACTTTCAG
GACTCCTGTCCCCCACTCCACAGGCTGCCTCCACTGGCAGGGGACTCAGAAGTGATCCGGTCACACTAAGTGACACTTA GTGATCAG
AAGTGCCCCGGTGCCACTGAGTGGCCTTGTCCAAGCTACATCCACTCTGTGGGCTCCTCCTTGTAGCAGCGAGGGGAGGGCAGATGT
CCCAGGGGCTGGTCACTGGAGCATTCCTCCCCTCTGACTCCCCAGTACAACGCCCTGCTAAGCAACATGAGCAGGATCTACTCCACC
GCCAAGGTCTGCCTCCCCAACAAGACTGCCACCTGCTGGTCCCTGGACCCAGGTACGGCCCTTGCAGCTCCCCTCTCGGCGGTGCCC
TAGTGTTCCCACATTGCCCTGCTGCACTCCGGACCATGCAGTTGTGTAGGGTCTGTGGAGACAGCAGGTAAACCCAAAGGTGTTGCC
CTCCAACTGGGGCTGGACGGTGCAGATACCCCCACGCCCTGCTTCTCTTGGCAAGTGGACTTCCGGAATCTCCAGCTGCAGCCCCCA
CTTCTGTGTGTACCTCGGCCTCTCCCATCACCCCTAGGCCTTCCTCCTGGCTGCCTGGTTTCCCCTTTC GTGGGTCCTCTCATGTTC
CCCAAGAGCCCTCAGGCCAGGGACCCCTCGTGGCTTCCCCTTAAACCCCGCTCCAGCCCCCTTTATGAGCAGCTTCGAGGAAGGCAC
TCCATCCAATAGGCCGCTAAGTGTCTGTCTGGGTTTTGGCCTTTGGGTGTCCCCTTGGTGTCAGCCACCTTAGGTGGTCATGTCTCT
GGGGCAGGGGCCCTGCCTGGGTGTTTCTGTAGCTCCCAGCCCCCTCCCACCAGGCCTGTAGGTGGCCCCTGTCTCTGGGGGCACCGT
GATGTTCAGGAAGCTGGTGGGAGCAGTAAGGACTGGTGCAGGCTCTGGTGAAGGCCGTTGAAGACTTCAACGTGGAGGCCTCCTCAC
CGACCCTGCCTGCCTGTGTCTCAGATCTCACCAACATCCTGGCTTCCTCGCGAAGCTACGCCATGCTCCTGTTTGCCTGGGAGGGCT
GGCACAACGCTGCGGGCATCCCGCTGAAACCGCTGTACGAGGATTTCACTGCCCTCAGCAATGAAGCCTACAAGCAGGACGGTGAGC
AGGCCTCTCCCTGTCCAGGAACCACGCCAGGTGTCCTCTCTCAGCTGTCTCCCCAGAGTCCCAGCCCAGAGTCAGGCAGAGCAGCTG
GTATGACAATTCCAGCAGGCCCTGAGTTTCCCAGAAAGTGGAGGTGGGACCGGCCTGCACCCAGTGTGCCTGGACTTTGCTGCTGGC
CTGCCCCACGTGGCCATCCTGCTGTCACTCCTGGCCCTGATGCTCCTCTTTGCCTCTGGGAACCTCCAGGATCTGTTTAGCTGGCTG
TAGCTAATTAGAAATTGTAGAGTGGCAACCCCCAAGCCAATTTTCCAGCTAGCTGCAGATCCACGGGCCTCGAGCCAGTGGAAGAGC
CGACTTACAGCTGAGAGGCTGAGGTCCGAGCCTTTGGCCTGAGCTACATACCTCACCCCCACGCCCCCAG GCTTCACAGACACGGGG
GCCTACTGGCGCTCCTGGTACAACTCCCCCACCTTCGAGGACGATCTGGAACACCTCTACCAACAGCTAGAGCCCCTCTACCTGAAC
CTCCATGCCTTCGTCCGCCGCGCACTGCATCGCCGATACGGAGACAGATACATCAACCTCAGGGGACCCATCCCTGCTCATCTGCTG
GGTAAGGACCTGGCCTCGCCTCCACATGAGTCCCACGGAAGTGTGGGTCCCGAGGTAGGGGTGGGGGATGTCCAGGGTAAGGGAAGG
TGGGTTGTGACCCTCACATCTCACATGTGTGGGGCATCATACTGTTTGCTTCACATGCAGGAGACCATTCGTGTTCCCACTTTACAG
GTGGGGACCCTGAGGCTTAGGGTCGTGAGGGACTTAGTGGTCAGAGAGCTAGGGGCCAAACCAAAGGCTCTGGCCCTGGGTCCAGTG
GGGGAGCCATCAGCCTAGCTCATGCCCAAGGAAACAAGCACTGTGGCCCTGCCTCAGGATTGAGTGGCTGGGGCCTGGCACAGCCAG
AAATGACAGTGGCAGCATCTTGCAGCCCCAGGACATGTGGCCCTCGGAGGAGTGTGGGTGGGACTGATGTGTGAGATTTCTGGCCCT
AAGCCAGGCCTGCAGCCCTTGAGGGCCCCAGGGTACAGGTGCCGGCCCCAGGGTGCCACTCAGCGATGCATGAAGAAGCAGGCACAG
CCAGGCAGGGAGCCAAGCTGTCCCCTTCCTTCCTTATCTAGGAGACATGTGGGCCCAGAGCTGGGAAAACATCTACGACATGGTGGT
GCCTTTCCCAGACAAGCCCAACCTCGATGTCACCAGTACTATGCTGCAGCAGGTAAGCTCTGGGCTCAAGCCTGGGGTGGTGGGGGT
CGGGGGTGGGGCGCAAAAAAAGGGAGTCACAGATGGGCACAGGGGCGGGAAGGTTTCGGGTACTGAGCAGCAGCCTGGTGTGTCTGT
AGGAGCAGTGAGCTGGGGTCGGCCCCCTCAGTGAGGTGCCAGCTCCTCCCTCCAGGCTCCACAGTGGCAGGATGAGAGCAACAACGC
ACTTTCACTCATCTGCTGTGGGAGTGAGGGCCCTGCCTCTGGGAATGGTGGCCACAGAGCAGAGAAGCTTTCATGCACAGGGAGTTG
ACCCGAGATGGGGACCCCAGCCCTGTCCCCAGGCCAGCCAGAGTGGGCTCCCCCTGACCTGGCTCCACACCCCTCCTCCAG GGCTGG
AACGCCACGCACATGTTCCGGGTGGCAGAGGAGTTCTTCACCTCCCTGGAGCTCTCCCCCATGCCTCCCGAGTTCTGGGAAGGGTCG
ATGCTGGAGAAGCCGGCCGACGGGCGGGAAGTGGTGTGCCACGCCTCGGCTTGGGACTTCTACAACAGGAAAGACTTCAGGTTCAGA
CATGGGAAGAGCACGTTCTGGGGTTCCCCGGTTCTGGGGCCCGGGGAAAGGCAGGCAGCCCAGGCGCAGGGAAGCTGGTTCCCAGGC
CTGCCTCTACCCTACCCCAGCACTGGTTGGAGGCTGGGTCTGTTCCAGGGCTAGGGGGTATAGGAGGCCTATTAGTCCACCTTCTCT
GGCAGCTTTGACAAATAGTCACTTCTATACCTTGGAATGGAGGAAGAAGGCCCAAGTGGTGGTGAGCCAGGGCAGGGTAAAGAATTT
GCTTGTTTCTGCCAGGCACGGTGGTCACACCTGTAATCCCAGCACTTTGGGAGGTCAAGGCGGGTGGATCACCTGAGGCCAGGAGTT
CGAGACCAGCCTGGCCAACATGGCGAAACCCCGTCTCTACTAAAAATACAAAAATAAATTAGCCAGGGGTGATGGCGGGCGCCTGTA
ATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCTCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGTGCCACTGCAG
GCCAGCCTGTGCAAAAGAGTGAGACGCTGTCTCAAAAAAAAAAAAGAAAAAAAGAAGTTACTTGTTTCTACTGCGGCTTCATGCCCC
AGGGCAGCTCCCTCCTCATTCCTGTCTTTCAGGTGCCAATCTGCCCTGTGCCCTGGCCCTGCCCTGTTCTGTCCATCCGTCACTCTC
ACCCTCGCCCTCTCTACGCCCCAGGATCAAGCAGTGCACACGGGTCACGATGGACCAGCTCTCCACAGTGCACCATGAGATGGGCCA
TATACAGTACTACCTGCAGTACAAGGATCTGCCCGTCTCCCTGCGTCGGGGGGCCAACCCCGGCTTCCATGAGGCCATTGGGGACGT
GCTGGCGCTCTCGGTCTCCACTCCTGAACATCTGCACAAAATCGGCCTGCTGGACCGTGTCACCAATGACACGG GTATGGGAGGGCT
GAGAGGCCCCCACCCAGCCTCACCTAAACCCCGCTCCACCCCACAGCAGGACCTCACTTGCCCCACTCAGCTCTGCCCTTCTTTCTG
CCTCCCGGCCCCAGGTCAGGCAGGGTTCGGGATCCTCCTAGAGCCTCACGGTGCACACTGCGCCCAGCTCAGCACACCTGGGGGTCC
TCTTCCAAGCAGGGCCCAGGGTCTCGAGGGCCAGCCATACCTTCTCTGCATCTCCCTGGCCTCACTTTCTGCTGCCCCGCCAGCCC A
CACTCTTAGGGGACCCTCTTCTCCCTCTGACCTCTTCCCTCTCCTTTCATCTCATCTCCCAACAGAAAGTGACATCAATTACTTGCT
AAAAATGGCACTGGAAAAAATTGCCTTCCTGCCCTTTGGCTACTTGGTGGACCAGTGGCGCTGGGGGGTCTTTAGTGGGCGTACCCC
CCCTTCCCGCTACAACTTCGACTGGTGGTATCTTCGGTGAGAGGAGGGATAGAAAAGCCTTCGCCCCAGCTAGCCCTCCCCAGCCTC
CTGGACAGCCAGGCGCCTCCTGCCCCAGCCAGTTCTAGCCTCTCCTCTCTAATGATGTCCCCCGCTGTGACCCACCGCCTTCTCCTT
TCCTGCCTGAAACTCCCTCTTCCAGGAAGTCTTCCCCAGTTCCTCAGGATGGGGAAGGGTTGCCGGGTGGAAATGCCTTTTCTACAA
AAGCTAAATCCATCTGTTTGCAACCTCTAGGCCCTAAGACAATTTAACCATCCTTTTCCAGAACCAAGTATCAGGGGATCTGTCCTC
CTGTTACCCGAAACGAAACCCACTTTGATGCTGGAGCTAAGTTTCATGTTCCAAATGTGACACCATACATCAG GTATTAGCGCCCCC
ACCCCACCCACCCCCAGTACTGTCACACCCTCAATCCACTTCTCCTCCTGTGATCCTAGCTGCCTCATCCCCAGGGCTTGTCCCCAT
GCTCCTCCAGACCTCAAAGGCCTGGAGTTAGAGTGGCCCACTCTCCTGAGCCTGTCTTGGGTCTCCCTTCT CCCCCAAGATAGCTTC
TGGTCCAGCCTCTGCCCTGCAGGAAGCTGGATGGTGCCTGGGTAAGGAACCCCTGTTCCTGGCCCCCCATGATCTTCCCTGACTCCC
ACCCTGTGCCTGCAGGTACTTTGTGAGTTTTGTCCTGCAGTTCCAGTTCCATGAAGCCCTGTGCAAGGAGGCAGGCTATGAGGGCCC
ACTGCACCAGTGTGACATCTACCGGTCCACCAAGGCAGGGGCCAAGCTCCGGTGTGTGGTGGGAAGCCGGGGGAAGTGGGAGGCAGA
GAGGAGCGGCTGGCAAAGGGTGTGGCAGGAGGTGTCTGGCTGCTCTGATGGGGTGGGGGGCACCAACCACAGAGCTGGACTGATGTG
GATGCCTGTCTCCTCGCTATGTCATCAAATATTTATTGAGTGGGCCTTCTGGCTGGCATGGGGCGACACAAATGCCCCCTGCCACCA
TCAGAGAGATCCCAGGCCCCAGGGTCTTATTGCCACAGTTTCTGCAGTCCATTGGGGGGCGGAAGTGGCCAGGGGCATGTGGGCCGG
GGTCCAGGAGCAGACTCCAGCCTGAGTCCCCTGTGCCCATGGTACCCACTCTGCCCACCAGGAAGGTGCTGCAGGCTGGCTCCTCCA
GGCCCTGGCAGGAGGTGCTGAAGGACATGGTCGGCTTAGATGCCCTGGATGCCCAGCCGCTGCTCAAGTACTTCCAGCCAGTCACCC
AGTGGCTGCAGGAGCAGAACCAGCAGAACGGCGAGGTCCTGGGCTGGCCCGAGTACCAGTGGCACCCGCCGTTGCCTGACAACTACC
CGGAGGGCATAGGTAAAGCCCTGAGTGAGGATGGTGTGGGGCTAAGGTGGGTCCTCAACTCTGGGCTTGGCCCAGGCCCCAGGTTCC
TGGTCAGCTCCTACCAGCTGAGCCCTGGTACCCTGTCCTGGAGGGCCAGGCAGCCCCCCAAGCTCATCAGCAGGGCCTGCGAGTGGG
GACAGGCATGTCTTTCCCCCAGCATCCTAGAGAGGGTGTGCTCAGACCTGAGGGCCCCTCCCCTTCCAGAGGAAGCCAGACACAAGG
CTCTGTGAGGTCACACTGCGGGCTCCGCTCTTATTGGCCAGGGGACGGTAGCTGCAGGACTCTGCTCTCCTGCGGCCATGGGCCAGG
GTTGGGCTACTGCAGGACTTCCCAGCCTCCTCTTCCTGCTGCTCTGCTACGGGCACCCTCTGCTGGTCCCCAGCCAGGAGGCATCCC
AACAGGTGACAGTCACCCATGGGACAAGCAGCCAGGCAACAACCAGCAGCCAGACAACCACCCACCAGGCGACGGCCCACCAGACAT Commented [AC2]: 7941 A/G
CAGCCCAGAGCCCAAGTGGGACCATGCAGGGGAGGGGCAGGGTGCCAGGGGTGGGAGAGGCGGGGCCGGGTAGGGACAGGGCAGGGT
ACAAGGGAGTGCGAGAGGGATAATGGCTTCTGGTGAGACCACAAACCTGGAGAGGGGAGGCAGAGGTTTGTCTGTTTCCCTGCACTC
TGTCCCACAGACCTGGTGACTGATGAGGCTGAGGCCAGCAAGTTTGTGGAGGAATATGACCGGACATCCCAGGTGGTGTGGAACGAG
TATGCCGAGGCCAACTGGAACTACAACACCAACATCACCACAGAGACCAGCAAGATTCTGGTGGGAGCCACCTCCCCACCCCCAAAC
CTGAGCATGTGCATACACACAGAGATGCTGTCCCGCTCACCACACAGTGGGGCTGCCACCACATTTTAAATTGAATATTTAAAACAA Commented [AC3]: 8342 C/G/T
TACTCAATTTCGGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGGGGAGGCGGGCGGATCACGAGGTCAGATC
AAGACCATCCTGGCTAACACGGTGAAACCCCATCTCTAGTAAAAATACAAAAATTAGCCGGGTGTGGTGGCGAGCACCTGTAGTCCC
AGTACTCAGGAGGCTGAGGCAGGAGAATGGCATGAACCCGGGAGGCAGAGCTTGCAGTGAGCCGAGATGGCACCACTGCACTCCAGC
CTGGGCGACAGAGCGAGACTCCATCAAAAAAAAAAAAAAAAAAAACTCAATTTCAGATTTTGATGAACATTTACTCAATGCCTGAGC
AATTCTTCTTTCCTTAAAAATCAGTCTCTGGGAGGCCTAGGTGGGAGGATCACTTGAAGCCAGGAGTTGGAGACTAGCCTGGGCAAC
ATAGCAAGATCCCATCTCTATTCAAACAAACAAATAAACAAAAATCAATCTCTAGTAACAGAATAATTTGTACATAAATAAGTGGTG
CTCAAGTCGTTTTTTAAAAGATTGAAAGCCTCTGTTTGTCTCCTCTACAAAAGGGGCTACACTTCCTCTTTACCCTCATTCCCTGCC
TATTTGGCTGAGCACAAATTATGCCACTGAGCCACACACTGTTACTGTTCCTTGGCACTTTGATCTGTTGCCTCATCTTTTTCTCAA
CAGCCTTGCAAAATTGGTGAGCTTATTCCCATTTTACAGATGGGATTTGATATTAACTCTGAGGTTCAGAAAGGCCACAGAGCTAAT
ACCAAGCTGGCTCCTTCCTAAGGGCCTTTAAGACACTTGGGGGTCTTCTCTTCTCTGCCCCTGCCTGGATATGTGTTGCTTGACCGC
AGGCATCCAGGGAGGGTGAGTACTGCATCCAGGACGTTATCAGCGTCCAGCTTGCAGAGAGTCTTATAGGCAAAGGTTGCAACTTAA
TTCCACTGCCCCCTCACCACCACCTCCAGCCCTCAGCTCCCACTTGGGGCCTCCCGCTCAGAGGCTGCTCTGGAGCTCCTGGGCCCT
GTGACACCATCCCCCTGTGCCCTCAGCTGCAGAAGAACATGCAAATAGCCAACCACACCCTGAAGTACGGCACCCAGGCCAGGAAGT
TTGATGTGAACCAGTTGCAGAACACCACTATCAAGCGGATCATAAAGAAGGTTCAGGACCTAGAACGGGCAGCACTGCCTGCCCAGG
AGCTGGAGGAGGTGTGTGGCTCGCAAGGTACAGGGAGAGGGGAATCCTGGGGCAGTGAGCCCAACACAGGGTCTGGCCTGGCCTTCA
CGCTGCTTCCTCTTCCTCGTTGTATCAAGTCATGGCATCTGCCATGCGATGTGCACCTCAGAACTGCTGAGAGGGCAGCGCTCCCCA
GCTCCCTGGCTCCCCACCTGCCAGCCCATGGGGCCTGGGGGTAGTGCAGGCCCCAGAGAGACCAAGTGCAAAGGAGTACAGCTCATT
GCCTCTCCTTCCTCCTGCAGTACAACAAGATCCTGTTGGATATGGAAACCACCTACAGCGTGGCCACTGTGTGCCACCCGAATGGCA
GCTGCCTGCAGCTCGAGCCAGGTGAGAGCTCATGTGCAGGCTGAGTGAGAGGCGAGGGCTGGGACTGGCATGGGGCCCGGGGGTGCT
GGGTGAGAGCACAGAGTTGGGTTCCCCTCGCTCTTGGGGTCAGCGTGCCCAGGAAATGCCCTTTCTTGTTTTCCACGAGGGGGGCTT
CTCTGCCCCACTGAGAGCCGGCACCTACTTCATACCATGCCCCGATCAGCTGCCCCTCCCTCAGAACCGCCCTCTGCTTAAG GGTGT
CCACTCTCTCCTGTCCTCTCTGCATGCCGCCCCTCAGAGCAGCGGGATCTCAAAGTTATATTTCATGGGCTTGGACTCCAAATGGGG
GGAACTCGGGGACACTAGCTCCCCCCGGCCTCCTTTCGTGACCCTGCCCTTGACTTCCTCACCTTCTCTGTCTTTCCTGAGCCCCTC
TCCCAGCATGTGACTGATAAGGAAATTGAGTCACACAGCCCCTGAAAGCGCCAGACTAGAACCTGAGCCTCTGATTC CTCTCACTTC
CCTCACCTACCCTGCCACTTCCTACTGGATAGAAGTAGACAGCTCTTGACTGTCCTCTTTTCTCCCCACTGGCTGGTCCTTCTTACC
CCGGCCCGTTTGAAAGAGCTCACCCCCGACACAAGGACCCGCACACAGATACCTCCCAGCTCCCTCTCAACCCACCCTTTCCAGGGT Commented [AC4]: 10593 A/G
TGGAGAACTTGAGGCATAAACTTGCTTCCATGAGGAATCTCCACCCAGAAATGGGTCTTTCTGGCCCCCAGCCCAGCTCCCACATTA
GAACAATGACAAATAGAAGGGGAAATGGAAAATAAACAGGAGAAACGGTTTTCCCAGGACAGGGTTTGGCCTACAAGTTGTGGAT GT
GGGTACCCATGCCAAGTGTGAGGGGAGGCTGGCCGGGTGTGGTGGCTCATGCCTCTAATCCCAGCACTTTGGGAGGCCAAGGTGAGT
AGATCACTTGAGGCCGGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCCATCTGTACTAAAAATACAAAAGTTAGCTGGGCG
TGGTGGTAGATGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCATGAGAATCGCTTGAGCCCAGCCTGGGCAATACAGC AAGACCC
CGTCTCTACAAATAAAATACAAAAAATTAGTTGGATGTGGTGGTGCATGCCTGTAGTCCTAGCTGCTAGGGAGGCTGAGATGGAAGG
ATTGCTTGAGCCTGGGAGGTCAAGGCTGCAGTGAGCCGAGATGGCGCCACTGCACTCCAGCCTGGGCAACAGAGTGAGACCCTGTCT
CAGAAAAAAAAAAAAAAAAAAAAAGGAGAGGAGAGAGACTCAAGCACGCCCCTCACAGGACTGCTGAGGCCCTGC AGGTGTCTGCAG
CATGTGGCCCCAGGCCGGGGACTCTGTAAGCCACTGCTGGAGAGCCACTCCCATCCTTTCTCCCATTTCTCTAGACCTGCTGCCTAT
ACAGTCACTTTTATGTGGTTTCGCCAATTTTATTCCAGCTCTGAAATTCTCTGAGCTCCCCTTACAAGCAGAGGTGAGCTAAGGGCT
GGAGCTCAAGGCATTCAAACCCCTACCAGATCTGACGAATGTGATGGCCACGTCCCGGAAATATGAAGACCTGTTATGGGCATGGGA Commented [AC5]: 11599 A/G
GGGCTGGCGAGACAAGGCGGGGAGAGCCATCCTCCAGTTTTACCCGAAATACGTGGAACTCATCAACCAGGCTGCCCGGCTCAATG G Commented [U6]: Fragmento sem a deleo intron 16
TGAGTCCCTGCTGCCAACATCACTGGCACTTGGGTCCCTTCATTTTCCTCAAAGAGGTGCTGTGAAACCCCAAGCCTAGGAAAAGGT
AGATCCCTGGAGGAGGCAGGTAATGTGGTGTTGGGAGAGCCTGGCTGTGTCCCCTCTGTAGGCTATGTAGATGCAGGGGACTCGTGG
AGGTCTATGTACGAGACACCATCCCTGGAGCAAGACCTGGAGCGGCTCTTCCAGGAGCTGCAGCCACTCTACCTCAACCTGCATGCC
TACGTGCGCCGGGCCCTGCACCGTCACTACGGGGCCCAGCACATCAACCTGGAGGGGCCCATTCCTGCTCACCTGCTGG GTAAGGGC
ACATGTCGGGCCTTGAGGAGGGTAAAGACGGACCACAGTGTGAGTGAGGGTTGGGACAGGGCTGACTAGAGGGTAGGGAGCAGGCTG
GGGACTGAGAGACTCCAGCCCTGTGGGGGATGGTTGCCCAGGCTGGAGGGGGGTGGGCGCTGGGAGTGGGGAGCCCCCCACTTGCAT
CTGGTGCCACATTCACTGCAGATCTATGTCGGGCAAGTCACCATGGATGGGGGAAGAAGTTAATAATCTTGTCCAGGAGACCACGGC Commented [AC7]: 12292 A/G
ACCCATCACAACATTGTGTGATCTTAGAGGGCGAGGAAGAGGCTGTGAGTGGGAGCTGGGGAGGCTTTGCCAAGAGGTGGCCTGTGA
GCAGGGCCTCGGAAGATGACAGGGTTTGACAGATGGGAAGTGGGGGATGAGAGGACAGACGCAGTGTTCAGGCCAAGGGAACTGGAA
CAAAGAAGAACCTGAGAATGTAAATCTACTTCAACCCTGGACCCTCCTTTGCCAAGGGCTGCAATCTCAGATGCCCTGAATGTGTGA
AGTAGGCGGTGAGGACAGTAAGGGATGGTAGGGAGTAAGGCAAAGCAGAGGCTACTGGTTCTCTGTCCCTGATGGGCTGTTAGGAAC
ACTTTCCTGGAGCAGAGAGACCAGACAGGCCCTCAGACCATTTAGAAACTATAAGGGAGGCCCCAGAGGACGGCCTGGCTGTGGGTC
TAGCTCCCACACAGGCTGGGAGTCCAGCCCTCTTCAGCCCCTCTCTGGTGAGACCAAAGAACATCTGGTGATGTCACAGTGGACGTC
AGTTCACCAACTGGGAGACACAGGCCCCGGGAAGAAAAGCAACATGCCCAGCGTGGCCTGGGAGCTGGGGCAGAGCTGGCCTTAGAA
CTCAGCCCCTGACAATTGGTAAAAGGGGAAAGGGGAGCAACCTAACACTGATGCGCTCTCTGTCTCTCTCTCTGGCTCTCTCCCTGG
CTCTCTCCCTCTTCTCTCTCATGTTCTCTCCATCACTCATCGCTCTAACCCTCTCTCACTGGTTTGCACTTTAGACTTCATCTGATG
TCAGCCGAAGCTTCACCTCACTTGGCTAGGAAAGAGCCTTGAGTCCAAATCTGTTTCTGAGCCTTCCATTCATCCTGAGTTTCTTCC
TTTTCCTCTGTCGTGGAGAACTAGGCTCTTTTCTTACACTAAACTCAGAGGCATCAGCCTCTCCTGAAGGAGACGGCTGGTTCCTGT
CAGAGTTGCTGAGCTGCAGACACCGACCTCAGGTGGTGCGGAGGGGACATGGCAGAGTGGCTGGTGAAGAGAGCAGCCTGCCAGCCT
TTCAATCCCAGCCCTGCCACTTAGGAGCCGTGGGCCCCCGGCGAGGGGGGCGGTCACTTAACTCTCCAGCCTGTTTCCTTTACTAGC
CAATGGGAATCGTGACAGTACCTGGGTGCAGACAGGATTGAAAGTGAATTCACACAATGTTCTTGGTGCAGAGCCAATAAGAGGTGG
CCACCGGGGTGTAGGTGTTCTGGGGACCTGTAATGTCCTCACATGTCAGCAGTTGCTAGTCACATTGGTCTCCACTGCTCACGGACA
GTGAAGACCACCTGGATTCCCTGATAACCAGCAAGGCCCCCACCTAGAGCCAGGCAGTAATGACCTACTGGGCTGGATATGCACACC
AAAGATGATGTGTGCCTCAAAGCTTGCAAACAGTAGGTGCTCAAGAAATGCCACTATGATTAGCAGGACTGGGATCTGGAGCGCTCT
TCCTGCAGGAGGGCATTGAGCCTAAGTAACATTTGTCTTTCCTCTCTCTGCCGTCCCCCACACTCGCCTCCAG GGAACATGTGGGCG
CAGACCTGGTCCAACATCTATGACTTGGTGGTGCCCTTCCCTTCAGCCCCCTCGATGGACACCACAGAGGCTATGCTAAAGCAG GTC
CGCACCAGCCCAGGGGCAGGGAGGCCCCGCCGGGATGGGAGGGACCCTCTGATTCAGGAGTTCCCTCCAGTTTAGCCCTCCCCCGGG
ATCCCCACGGCAGCACGCAGTCTGTCCCCGGAACCCCCAGTTTGGGCAGAACTCCCTCTGCTTGCAG GGCTGGACGCCCAGGAGGAT
GTTTAAGGAGGCTGATGATTTCTTCACCTCCCTGGGGCTGCTGCCCGTGCCTCCTGAGTTCTGGAACAAGTCGATGCTGGAGAAGCC
AACCGACGGGCGGGAGGTGGTCTGCCACGCCTCGGCCTGGGACTTCTACAACGGCAAGGACTTCCG GTACATCCAGCTAGGGCTCAG
GTCTCGTTCCTGAGCCCCACGGGCAAGGGAAATGAACCAAGCAAAGGGTCCACTACTGTCCCCCAGCTGGAGCCAGCAGGGCAGGAT
GGGGACAGGGCCAGAGTTTGGGACTGAGTGTCTAGAGAGGTGTTGGCTTCTGGCAGGAAAACCCCATCCGCCTGATGGGGACTTCTG
AAGCACGCAACAGCTCTGTCAGCCTGGCCGCTGGGAAGTGCTCAAGGTCCCAGTCCTGGGTTTGAGCATGGTAGGCTGCCCCGCGTC
CCTCCTTGGGAGCAGCCCCTGCATGGAGCTGGCCTCTCCCTGGGGGCACATGCTGTGACACAGGGAGGCACACGAGGATGTTGGGTG
CTCTGTACAGATCCACTCTCACCCCTGACAGGCTCAGAAGCTGCCTTCCTTGGAGGATGGCGTTTTAGTTACCTATTGCTGTGCAAC
CAAGCACCCCAGAGCTTAGCCTTACGAAACAACCAGTTGATTTTGCTTATGATTTTATGTGCCAGGAATTCAGGCAGTACACAGTGG
AAATGGCCTTTCTCTACAGGGCCCCCTCTGTTGGGGGCAGCTCTCACAGCCGGGATGGCTCAATGGGGGCCATACGTCCAGAG CCCC
AGTTCTGGCTGTCGGTTGAGGTCCTCGGTTTTTCCCATGTGGCAGATGCTGGGGCACATGTTCCCAGTGGCCTCTTGGCTCACATGT
TGGGTGCTTGGGGTGAGATGGCTGGAACGGCTGGAGGTGGGTCAGGCATCTCTCCAGGCTAGCTCGGGCGCCCTCCCAGCGTGGAAT
CTGAGGTGGGCAGATTTACCTGGCAGCCAGCATCCCCCACAGCAACTACTGACGCAGCCAGTTCTCAAGGCTAGGTCC AAAACTGGC
CCAGAGTCACTTCTGCCATGTTTTATTGGCTAGAACAAGTCACAAGTTCACCCAGATTCAAGAGAAGAGAAAAAAGTCCCTCCCACT
TGGGAGAAGTGGCAAAGACCATCTGTCACAGCTGAAGAAGTGTCTCTTACAAGGAGAACAGACACGGGGAGCCTGAAACAAAACCCG
ATGGGATTCCCTGGGCTGTGCAGGCCCTTCCAGGCATGAGGACTCAGCCACAGGGCTGAGAGGAGACAGGATC TGGGGGATGAGAGC
CCTTGTGGGGTCTTCCCTTTTATGGGGAGTCAGAGGAGAAGCTGGATAGATCCCCAGCCTTGTGGCCAGGATGCTGGGCAGCTCTTC
CTTCCCCCTCCCCGATGAGAATGACAGAAAAACAGGATTCACCTGAGCCAAAAGGCTTCCAGTTAGATCCAAGAGAGAATTTCCCGC
AGTTTGAATTGGTTTGCTAAACAACAAGGAAGGGCTGGGTGCGGTGGCTCACACCTGTAATCTCAGCA CTTTGGGAAGCCGAGGCAG
GAGGTCTGCTTGAGCTCAGGGGTTCGAGACCATCCTGGGCAACATAGCGAGACCCCATTTCATAAAAAATAAATAAGTAAATGAGAA
CAAGGAAGGACTGACGAGAGACGGTAGAACCTTCTGGTTTGGGCAGCTCTGCAGCTGCCATTCATCCTGGCCATAAGAATTCTTGGG
GTGAATAAGTTTGTCGCTGTTGGGCCGCATGAGATGCAGAATCGCCCACTCTCACCCCTGACAGAAACAGTTGTTTCCTTCAGGGAG
CCTCCATCTTGGGAGATAAAGCATGTGTACATGGGAACCCACTGGCCACACATTCTCTAGAAAGTACACAATGTCCCAGTGCCTCTA Commented [AC8]: 15990 A/G
GAGCAAGCACTTTGTACAGTCAGAAAGCAACAGGTGGTGGGGGCTGGAGTCATTCAGGAAAATGGGAGGCAGAGGAATGGCCTGAAC
GGCCCGATGCTAGGGGCTTCTGCCCCCAGATTCCCTCTTACGCACACTCAGTGGTTGCCCTTCCCCTCCCTCCCCACAGTGCTGTGT
CCCCTGCATGCTGCAGTGCTGGGGTCTGCCCTGGGTATAGCAAGGCCCACTGTTCCCTTATGCCCAGGGCTTCTCACTGTCCTCTCC
CAACACCCTCTCCCCCACTCCACTATTCCTAGGATCAAGCAGTGCACCACCGTGAACTTGGAGGACCTGGTGGTGGCCCACCACGAA
ATGGGCCACATCCAGTATTTCATGCAGTACAAAGACTTACCTGTGGCCTTGAGGGAGGGTGCCAACCCCGGCTTCCATGAGGCCATT
GGGGACGTGCTAGCCCTCTCAGTGTCTACGCCCAAGCACCTGCACAGTCTCAACCTGCTGAGCAGTGAGGGTGGCAGCGACG GTGAG
AGAGAAGCGGGAGGCCCTGGTGGGCTGAGGACCAAGAAAGGGTGGTGAGCTTGGGAGGTGGGAAAGGGGCACTTAGTGGCCCATGGG
CAGAGGTGTGGGGCAGAGCAATCGGAAGGAAGGGAGCCACCCAGACCATCCCAGGAGGCAGGTCACAGGGCCCAAAAGGTACAGCAC
CCCCACCCCTCCACCATCACAGGCACACCAGGGCCAAGCCGCTAGGACCCTGGGTCTGACAGCTGGGCTCCCTTCCCTTGCAG AGCA
TGACATCAACTTTCTGATGAAGATGGCCCTTGACAAGATCGCCTTTATCCCCTTCAGCTACCTCGTCGATCAGTGGCGCTGGAGGGT
ATTTGATGGAAGCATCACCAAGGAGAACTATAACCAGGAGTGGTGGAGCCTCAGGTTCTGGAACACTCCCACGGGATGCGGGCTGGG
GGATCTCTGCGAGTGTCTGCATGTGCCTGGGTGTCTGGATGGGCCAGGGTAGGGGAGTGTGTGTGTGTGTGTACACTATTGTGTCTG
TGCATATGATGTGTGTGGTGTAAGTGATGGGGAAAACGGGGTGATTGTGCACAGAGGCCCAGCACGCAGGAGAATGGGGTGCCCAGT
ATAGCCCCAAGTGCAGGGACCCTCCCTCAAGTCAAAAATGCCACCCCCAGCCTGGTTCTCCCCAAACTCATCTTCCAACATATATTC
CCACTCGACAGGCTGAAGTACCAGGGCCTCTGCCCCCCAGTGCCCAGGACTCAAGGTGACTTTGACCCAGGGGCCAAGTTCCACATT
CCTTCTAGCGTGCCTTACATCAGGTAACGGGAAAGGCAGGAGGGCACATTGTGAGGGGCAGTACCCACAGCTTTGTGTTTCAACTGC
GGCCACTGCCCGGTCCACAAGCTCTGTCAGTCAGGGCAGACCCGGGGGAGCCGGCCGCACGGTGCAGGTGCCTGGGCCCACTCACAC
TGCCAAGGCTGATGGGTTTTTTCTTGAACATTCTTTTGATGAGAGTCTGTACCATCCAAACAGTTGAACACAGAAACTCAACCTAAT
AATTGGCTAATGGTTACCAGACCTTGGTTAAGTAGTTAACATTAACCACGACTCATGGCTGGATCATGAGCTCTGCACTGTTTTGTT
TTGCTTTTAAAACAAGACTGTGATTCTTTTACTATTATTGAACATTGTCTGCGATACAATTTGAATTGTACCTGGAAGCCCTTCTAG
ACACTAAAATGTAGGATTGGAGATCGGTTAAGGTGGGAGGCAGGGTTGCTGGGGCAAGTTACAGTCACAGGCTGGGGTCAGACAGAA
CTGGGTTCAAACTCCGTCTCCATTACTTTGTTTCCTTGAGAAAATTCCTCAATTTCTGTGAGCTTCCATTTCCTGACCTGTGAACCC Commented [AC9]: 17911 C/T
CATTTCACAGGATGCACGATGGCTAACTTCTTAGCATTCTGTCTCATACACAGCCTCCTCAGGGAGGGGTGGCCAGGACCCCACTAT
TCATCACTCTCTAGTGGAATGTAGCTGCACACTAGGTCTGCAGGTCACATGGCCACAGATGAGTGTGCCCAATGCAGCCCCTCTCCT
TCTGTGTGCCCCGGGAGAGCACTTGCTGAGGGCTAGCAAGGCTGTTTGTGATCCGGGAGGCTCCCTGGGAGGCTGGGGGCTAGAGAG
ACCTCAGGCTGGAGTTCCAGGTGCCCCCGGGCTACAGGTAGCCCAGGCCACCCCCAGAGGGCTGTGGCTGCCTCTGGCCCTGGCCTC
CCGTGGTTCCTGGAAGCCCAGCAAGGGCAGGGCCCATGCCCACCTTGCCTCCTGGCACCTGGGATGATGCCAGCACATCATCAAGTG
CTAATAACTGATTGTGGGATGGATGAAGTCTGTCCCCAGAGTCCAGGAAGAGGGCATCCCTGGAGCACCTCAGATAGGCCTGACCTA
GACGGTGCTCCAGAGATGACACTTAGGACAGGGCTCCCGCCTGCCTGCTGGAGTGGTCCCTGGGGTTCCCAGCCGGCGCTGGCTCTC
ACCCGGGAGCCAGCTGGTGTGATGGCTAGCTTCCCAGCTTAATGCAGACAATTCTCCAAACAGGGGTGGGCAAAGGAGACTTGGCTG
CTCTAGAAAAACATTCCGGATTCTGGCCAGCAGCCTTCACAAAGCACTTTTAGGAAAGACCAGGGAACTAGGTGGTACATGCTTGCA
CCCAGCATTCAAAGTGAGAGGCCTGTGCCACTGGCTCAGGACATTTAAAACCTCTTCAGACTTTAAGCTGGGGAGAATCCTCCAGCC
TTGACTGGCAGATTTCTACCAGGGAATTCGTGATGCTTTGGATAAGATCATGTAGGACTGGCTTCCCTGCCCAGACCACCCTAGTAG
ATCCACACGGCCCATTTGGCCACACCTTGCCTCTACTTGCATATACCCCAGGGATGGAGACCTCACTGCCTCCAAAGCCACCTG CCA
GATCTCTGAAGGCTCTGCCCTGACCCCATGGAGCAGGCCCTCCTGAGTTGTGGAGGCAGCTCTGTGGGTGGGAGGCATCTACACAGG
CACGGCTAGGAAGAGGCTCAGACAATGCTAAGAGCTGGGGTGGGGGAGCTCACCCTGATAGCTGTGGGCAGAGTTGGGGGGCCTTGG
CTCTGCTGTGCGCATGTGACTTAGCACACATCACATGTGATGTGCAGAAGGGCCTGGGGCCCAGTGGCACAAGGCCCTC AACCAACT
CCGCCCCGGGCCACGGCCTCGCTCTGCTCCAGGTACTTTGTCAGCTTCATCATCCAGTTCCAGTTCCACGAGGCACTGTGCCAGGCA Commented [AC10]: 19329 C/T
GCTGGCCACACGGGCCCCCTGCACAAGTGTGACATCTACCAGTCCAAGGAGGCCGGGCAGCGCCTGGC GTGAGTGTCCTCCAGCCCT
CCTTTGTTTCCATGCTCTGGCCTGCGCCCCTGGGCCTTGAGGGGTCTGTCCACTGGAGCTTTTGTGGGAAC ACTTGCCATTTTGAGC
CGGGAACTCCCACCTGCAGCGTGGGCCAGGCCTGATTGCCATCTCCTTAGGCACCTGGAGCCCTGGGGCCCTGGGACAAGTTTCAGC
TGGGAGTGGGTATGGAGAGTGGATGTCAGGTGGGGGCAAGAGGGGCCATGTCCTTCTGACTCTGCCTCCCTGTCTCATGCCTCCCCA
GGACCGCCATGAAGCTGGGCTTCAGTAGGCCGTGGCCGGAAGCCATGCAGCTGATCACGGGCCAGC CCAACATGAGCGCCTCGGCCA
TGTTGAGCTACTTCAAGCCGCTGCTGGACTGGCTCCGCACGGAGAACGAGCTGCATGGGGAGAAGCTGGGCTGGCCGCAGTACAACT
GGACGCCGAACTCCGGTACCGCCACCCACCCCACCTCCAGCCTTGGGTCTTAACCCCCTCCCCAGGCTGGGCAGCCATGCGGCTGAC
CTCGGAGCCTGGCCCTGCCCCGCACCCTTGCCCTGCCCTGCCCTGCCCTGCCCATGCTGTCTCCTTGCTTCCCGCTCAGCTCGCTCA Commented [AC11]: 20060 A/G
GAAGGGCCCCTCCCAGACAGCGGCCGCGTCAGCTTCCTGGGCCTGGACCTGGATGCGCAGCAGGCCCGCGTGGGCCAGTGGCTGCTG
CTCTTCCTGGGCATCGCCCTGCTGGTAGCCACCCTGGGCCTCAGCCAGCGGCTCTTCAGCATCCGCCACCGCAGCCTCCACCGGCAC
TCCCACGGGCCCCAGTTCGGCTCCGAGGTGGAGCTGAGACACTCCTGAggtgacccggctgggtcggccctgcccaagggcctccca
ccagagactgggatgggaacactggtgggcagctgaggacacaccccacaccccagcccaccctgctcctcctgccctgtccctgtc
cccctcccctcccagtcctccagaccaccagccgccccagccccttctcccagcacacggctgcctgacactgagccccacctctcc
aagtctctctgtgaatacaattaaaggtcctgccctcccCATCTGAGTCTGTGTCCCTCACAGGGAAGCCAGGGACAGGGACAGGCT
GCTTTCCTGCCTCCTGGCAGTCAAGTGGGTCCCGTTACTAGGTTTGTTCCTCCATCCTCCTTCAGGAGCCGGGGAGGATCCCCAGAG
CTCTGCCCCAGCACCTCCTGGCGCTGGCGCCTGTCTTCCCTCCAGCCCAGGCAGCCCGCCACTGTCCTGCCACCGCAGGCAGCCCCT
GTCTGGCCCAAGCACTGACCCACGCGGACTCTGGGAAGCAGACATCCTGGGCTGCTGGCCTCACATTTCCACTGGCAGTGGAGCCTT
TCCCTGCTCCACAAATGGCCAGGTCCCCCCAGGGGAAGGCTTCCGGCTGTTATCGGCTGCCTCAGGGGGCGAGTACCTTGGAGGGCC
TGCTTCAAGGAGGGTGCCCCCTGGAGGGCACACACCAGCCTAGTGCTTACCTTGGCTCCTGCCTGTACCAGCTCCATGA CTCTGCTC Commented [U12]:
GGGTGAACAGCCTTGGCTCTCAGACAGCCATTCTAACACTGCCAGTGCAGAGGGGCCTCAGACGCTGGAGTGTAGCAGTGGCTGCAC
CTGCACAGGGATTAGCTGCCAGCAGCCACCCTGCTGGCGTCCCAGCACACACCTCCTCACTCCCTGCATTGGAGGGAGTGTCATTTT
AAGGGACATTTTTATGACTTTTATGTGTATGTTTATGTAGAAATTTGGAAAATACAGAAAACTGTAAAGAAAATAAAAGCCCTTTAT
ATCAACGTCAAGAGATAAGCCCTGTTGACGTTTTGGTGTACAACTTGCCGGACTTCTTCTCAGCACATGTGTAT

V RESULTADO FINAL

MUTAO SELECIONADA
CDON 20
NORMAL TTG LEUCINA
MUTANTE TGG TRIPTOFANO

CTCGGCCCGCGCCGCTGGGGACTTTGGAGCGGAGGAGGAAGCGCGGCGGGGCGGGGGCGGGGGTGTGTCGGGTTTTATAACCCGCAG
GGCGGCCGCGGCGCAGGAGAAGGGGCAGAGCCGAGCACCGCGCACCGCGTCATGGGGGCCGCCTCGGGCCGCCGGGGGCCGGGGCTG
CTGCTGCCGCT[GCCGCTGCTGTTGCTGCTGCCGCC]GCAGCCCGCCCTGGCGTTGGACCCCGGGCTGCAGCCCGGCAACTTTTCTG
CTGACGAGGCCGGGGCGCAGCTCTTCGCGCAGAGCTACAACTCCAGCGCCGAACAGGTGCTGTTCCAGAGCGTGGCCGCCAGCTGGG
CGCACGACACCAACATCACCGCGGAGAATGCAAGGCCAGGTGGCGCCCGGGCCCGGGCGGGGGCGGGGCGGGCCGCGGCGGCCAATC
AC

Primer3 Output

No mispriming library specified

Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 60 19 59.52 52.63 2.00 2.00 GGGGTGTGTCGGGTTTTAT
RIGHT PRIMER 269 20 59.62 50.00 4.00 3.00 GCCTCGTCAGCAGAAAAGTT
SEQUENCE SIZE: 435
INCLUDED REGION SIZE: 435

PRODUCT SIZE: 210, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 186,24

1 CTCGGCCCGCGCCGCTGGGGACTTTGGAGCGGAGGAGGAAGCGCGGCGGGGCGGGGGCGG
>

61 GGGTGTGTCGGGTTTTATAACCCGCAGGGCGGCCGCGGCGCAGGAGAAGGGGCAGAGCCG
>>>>>>>>>>>>>>>>>>

121 AGCACCGCGCACCGCGTCATGGGGGCCGCCTCGGGCCGCCGGGGGCCGGGGCTGCTGCTG

181 CCGCTGCCGCTGCTGTTGCTGCTGCCGCCGCAGCCCGCCCTGGCGTTGGACCCCGGGCTG
************************

241 CAGCCCGGCAACTTTTCTGCTGACGAGGCCGGGGCGCAGCTCTTCGCGCAGAGCTACAAC
<<<<<<<<<<<<<<<<<<<<

301 TCCAGCGCCGAACAGGTGCTGTTCCAGAGCGTGGCCGCCAGCTGGGCGCACGACACCAAC

361 ATCACCGCGGAGAATGCAAGGCCAGGTGGCGCCCGGGCCCGGGCGGGGGCGGGGCGGGCC

421 GCGGCGGCCAATCAC