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XFlex
HydroFlex Service Software
Notice
Every effort has been made to avoid errors in text and diagrams; however,
Tecan Austria GmbH assumes no responsibility for any errors that may appear in
this publication.
It is the policy of Tecan Austria GmbH to improve products as new techniques
and components become available. Tecan Austria GmbH therefore reserves the
right to change specifications at any time with appropriate validation, verification,
and approvals.
We would appreciate any comments on this publication.
Manufacturer
Tecan Austria GmbH
Untersbergstrasse 1A
A-5082 Grdig/ Salzburg
AUSTRIA / EUROPE
T: +43 6246 8933
F: +43 6246 72 770
www.tecan.com
E-mail: office.austria@tecan.com
Copyright Information
The contents of this document are the property of Tecan Austria GmbH and are
not to be copied, reproduced, or transferred to another person or persons without
our prior written permission.
Copyright Tecan Austria GmbH
All rights reserved.
Printed in Austria.
2 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
Remarks on Screenshots
The version number displayed in screenshots may not always be the one of the
currently released version. Data and parameters displayed in screenshots may
vary depending on the instrument connected. Screenshots are replaced only if
content related to the application has changed.
Trademarks
The following product names and any registered and unregistered trademarks
mentioned in this document are used for identification purposes only and remain
the exclusive property of their respective owners:
HydroFlexTM, XFlexTM, Tecan and the Tecan Logo are registered
trademarks of Tecan Group Ltd., Mnnedorf, Switzerland
Greiner is a registered trademark of Greiner Labortechnik GmbH,
Frickenhausen, Germany
Pentium is a registered trademark of Intel Corporation,
Santa Clara, CA, USA
Windows ,MS DOS and Excel are registered trademarks of
Microsoft Corporation, Redmond, WA, USA
Note
Gives helpful information.
Caution
Indicates a possibility of instrument damage or data loss if instructions
are not followed.
WARNING
INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS
OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT
FOLLOWED.
WARNING
THIS SYMBOL INDICATES THE POSSIBLE PRESENCE OF
BIOLOGICALLY HAZARDOUS MATERIAL.
PROPER LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED.
WARNING
THIS SYMBOL INDICATES THE POSSIBLE PRESENCE OF FLAMMABLE
MATERIALS AND A RISK OF FIRE. PROPER LABORATORY SAFETY
PRECAUTIONS MUST BE OBSERVED.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 3
Table of Contents
1. Safety .................................................................................................................7
1.1 Instrument Disinfection .......................................................................7
1.1.1 Disinfection Procedure ...........................................................................7
1.1.2 Disposal of Operating Material ...............................................................8
2. Introduction .......................................................................................................9
2.1 Specifications .......................................................................................9
2.1.1 Hardware Requirements .........................................................................9
2.1.2 System Requirements ............................................................................9
2.2 Installation ..........................................................................................10
2.2.1 Installing the Program...........................................................................10
2.2.2 Uninstalling the Program ......................................................................11
2.3 Starting XFlex .....................................................................................12
2.4 Connecting the HydroFlex Washer ................................................... 13
3. General .............................................................................................................17
3.1 Main Window ......................................................................................17
3.1.1 Header ..................................................................................................18
3.1.2 Menu Description..................................................................................18
3.1.3 Toolbar Description ..............................................................................21
3.1.4 Tabs/Control Pane and Info Pane Description ..................................... 21
3.1.5 Status Bar Description ..........................................................................22
3.2 Log Entry .............................................................................................22
3.3 Exiting XFlex .......................................................................................22
4. XFlex Menu Options ........................................................................................23
4.1 File Menu .............................................................................................23
4.1.1 Change User ........................................................................................23
4.1.2 Options .................................................................................................23
4.1.3 Quit .......................................................................................................23
4.2 Instrument Menu.................................................................................24
4.2.1 Disconnect/Connect .............................................................................24
4.2.2 Prime ....................................................................................................24
4.2.3 Rinse ....................................................................................................25
4.2.4 Send Command....................................................................................25
4.3 Store/Restore Menu............................................................................26
4.3.1 Instrument Settings...............................................................................26
4.3.2 Save Plates and Programs ................................................................... 28
4.4 Setup Menu .........................................................................................29
4.4.1 Serial Number.......................................................................................29
4.4.2 Firmware Download ..............................................................................30
4.4.3 Language Download .............................................................................32
4.4.4 Instrument Configuration ......................................................................32
4 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4.4.5 Default Offsets ..................................................................................... 33
4.5 Options Menu ..................................................................................... 34
4.5.1 Process Control Parameters ................................................................ 34
4.5.2 Process Control Test ............................................................................ 35
4.5.3 LLD Test............................................................................................... 36
4.5.4 Low VAC Parameters ........................................................................... 37
4.5.5 Low VAC Test ...................................................................................... 38
4.5.6 High VAC Test ..................................................................................... 39
4.6 Function Test Menu ........................................................................... 42
4.6.1 Y, Z Motors........................................................................................... 42
4.6.2 Dispense Pump .................................................................................... 43
4.6.3 Aspiration Pump ................................................................................... 43
4.6.4 Channel Valve Test .............................................................................. 44
4.6.5 Bubble Sensor Test .............................................................................. 45
4.6.6 Burn In.................................................................................................. 47
4.7 Adjust Menu ........................................................................................ 48
4.7.1 Y Motor Resolution (depending on FW and material number) ............. 48
4.7.2 Y/Z Motor Offsets ................................................................................. 50
4.7.3 Plate Sensor Offset .............................................................................. 52
4.7.4 Adjust Prime Position ........................................................................... 53
4.7.5 Bubble Sensor Calibration .................................................................... 54
4.7.6 Dispense Pump Calibration .................................................................. 56
4.7.7 Dispense Rate Calibration .................................................................... 57
4.8 End Test Menu .................................................................................... 58
4.8.1 Verify Motor Positioning ....................................................................... 58
4.8.2 Plate Sensor Offset Test ...................................................................... 59
4.8.3 Rinse Position Test (depending on FW) ............................................... 60
4.8.4 Prime Position Test .............................................................................. 61
4.8.5 Verify Dispense Capability ................................................................... 62
4.8.6 Verify Aspiration Capability .................................................................. 63
4.8.7 Verify Aspiration Capability MBS (depending on material number) ...... 65
4.9 Print Menu ........................................................................................... 67
4.9.1 Print ...................................................................................................... 67
4.10 Help Menu ........................................................................................... 68
4.10.1 About XFlex ...................................................................................... 68
Abbreviations .......................................................................................................... 69
Index ......................................................................................................................... 71
Tecan Customer Support........................................................................................ 74
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 5
1. Safety
1. Safety
1.1 Instrument Disinfection
It is very important that the instrument has been thoroughly disinfected before
performing the tests provided in this IFU.
WARNING
THE DISINFECTION PROCEDURE SHOULD BE PERFORMED
ACCORDING TO NATIONAL, REGIONAL, AND LOCAL REGULATIONS.
WARNING
ALL PARTS OF THE INSTRUMENT THAT COME INTO CONTACT
WITH POTENTIALLY INFECTIOUS MATERIAL MUST BE TREATED
AS POTENTIALLY INFECTIOUS AREAS.
WARNING
RISK OF FIRE AND EXPLOSION!
PRIOR TO CLEANING THE OUTER SURFACE OF THE INSTRUMENT
AND THE DISPLAY, SWITCH OFF THE INSTRUMENT AND DISCONNECT
IT FROM THE MAIN POWER SUPPLY!
ETHANOL IS FLAMMABLE AND WHEN IMPROPERLY HANDLED CAN
LEAD TO EXPLOSIONS AND/OR FIRE. PROPER LABORATORY SAFETY
PRECAUTIONS MUST BE OBSERVED.
Caution
Before starting the disinfection procedure
use a rinse procedure (Rinse Day)
with distilled or deionized water (lab quality) to flush the system.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 7
1. Safety
WARNING
THE DISINFECTION PROCEDURE SHOULD BE PERFORMED IN A
WELL-VENTILATED ROOM BY AUTHORIZED TRAINED PERSONNEL
WEARING DISPOSABLE POWDER-FREE GLOVES, PROTECTIVE
GLASSES AND PROTECTIVE CLOTHING.
Please note that the disinfectant can influence the performance of your
instrument if it comes into contact with the electronics!
The following procedure should be used to disinfect the instrument:
1. Wear protective powder-free gloves, protective glasses and protective
clothing.
2. Prepare an autoclave bag for all disposables used during the disinfection
procedure, label it with autoclave tape and put it in the autoclave or follow
the procedure defined in chapter 1.1.2 Disposal of Operating Material.
3. Prime the liquid system with disinfectant. Perform the Rinse - Night
procedure with disinfectant solution and stop the procedure after five
hours.
4. Switch off the instrument and disconnect the instrument from the main
power supply.
5. Disconnect the instrument from any accessories that are used for
example: Liquid Level Detection System, computer, etc. Accessories that
should be shipped together with the instrument have to be included in the
disinfection procedure.
6. Carefully spray the disinfectant solution (or use a disposable soft tissue
paper towel soaked in the disinfectant) on all outer surfaces of the
instrument (continued on next page).
7. After a minimum contact time of 10 minutes, repeat step 6 of this
procedure.
8. Wipe dry the outer surfaces of the instrument.
9. Wash your hands with a mild detergent and then disinfect them.
WARNING
CHEMICAL AND BIOLOGICAL HAZARDS CAN BE ASSOCIATED WITH
THE WASTE MATERIAL (MICROPLATE) OF PROCESSES RUN ON THE
HYDROFLEX.
TREAT THE USED MICROPLATE, WASTE BOTTLE, PRIME TRAY ON
THE PLATE CARRIER, DISPOSABLES AND ALL SUBSTANCES USED,
IN ACCORDANCE WITH GOOD LABORATORY PRACTICE GUIDELINES.
INQUIRE ABOUT APPROPRIATE COLLECTING POINTS AND
APPROVED METHODS OF DISPOSAL IN YOUR COUNTRY, STATE OR
REGION.
8 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
2. Introduction
2. Introduction
2.1 Specifications
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 9
2. Introduction
2.2 Installation
Caution
STOP It is very important that the person who installs the XFlex service
software has System Administrator rights on the computer.
10 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
2. Introduction
CAUTION
STOP IT IS VERY IMPORTANT THAT THE PERSON WHO UNINSTALLS THE
XFLEX SOFTWARE HAS SYSTEM ADMINISTRATOR RIGHTS ON THE
COMPUTER.
1. The following message will appear asking you if you really want to
remove the program. Click Yes to continue.
2. The following message will appear asking if you would like to remove all
created configuration and instrument data files. Click Yes or No.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 11
2. Introduction
It is possible to use XFlex with instruments with firmware versions lower than 2.0;
however this is not recommended, as some of the tests will not be able to be
performed. It is recommended to update the firmware version of the instrument to
Version 2.0 or higher.
12 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
2. Introduction
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 13
2. Introduction
Select Install from a list or specific location (Advanced) and click Next to
continue. The following dialog box appears:
Select Dont search. I will choose the driver to install. and click Next to
continue.
14 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
2. Introduction
Select Show compatible hardware and click Next to continue. The necessary
configuration tasks are performed. Once all the tasks have been performed
successfully, the following dialog box appears:
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 15
3. General
3. General
3.1 Main Window
The Main Window consists of the following items which are described in detail in
the subsequent chapters:
Header
Menu bar
Toolbar
Tabs/Control pane
Info pane
Status bar
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 17
3. General
3.1.1 Header
The Header contains the name and version number of the software as well as the
standard Windows dialog box buttons.
18 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
3. General
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 19
3. General
20 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
3. General
Button Explanation
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 21
3. General
Enter the User name and the reason for performing the test or adjustment.
If a part has been replaced, select Test after replacing part and enter the name
of the part in the field.
If no parts were replaced, select Other and enter the reason for performing the
test or adjustment in the field.
22 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
4.1.2 Options
Select Options from the File menu to open the Options dialog box:
The paths of the Configuration File, DHR Main Directory, Firmware Download
and Language Download can be viewed.
The paths can also be modified by clicking the Browse button and browsing
for the desired folder.
Click OK to save changes or Cancel to discard changes.
4.1.3 Quit
Select Quit to exit the XFlex software.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 23
4. XFlex Menu Options
4.2.1 Disconnect/Connect
Select Connect/Disconnect from the Instrument menu to connect or disconnect
the instrument.
4.2.2 Prime
Select Prime from the Instrument menu to prime the instrument.
Select the Channel (1 -4), duration Time (5 99 s) or Volume (15 820 ml) and
click Start to begin the priming procedure.
Click Stop to abort the procedure. When the prime procedure has ended, the
Prime dialog box closes automatically.
24 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
4.2.3 Rinse
Select Rinse from the Instrument menu to perform a rinsing procedure.
Select the Mode (Day or Night), the Channel (1 -4) or duration Time (5 99 s)
or Volume (15 820 ml) and click Start to begin the rinsing procedure.
Click Stop to abort the procedure. When the rinse procedure has ended,
click Close to return to the main window.
For example, enter ?VERSION in the Command String field and click Send to
send the command.
The response appears in the Received Data window, e.g.
<< ?VERSION (<< marks commands sent)
>> V_1.10_03/07_HYDRO Mar 28 2007 12:54:01 (>> marks responses)
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 25
4. XFlex Menu Options
26 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 27
4. XFlex Menu Options
28 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2. Select the Instrument Type from the drop-down list. The Serial Number
has to be entered manually. The Material Number and Instrument Alias
fields will be filled in automatically. If your instrument is not listed, select
Other and fill in the fields manually.
3. Click Set to confirm changes and the following dialog box appears:
4. Click OK, switch the instrument OFF and ON again and reconnect the
instrument to the software.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 29
4. XFlex Menu Options
3. Select the file type from the list under Download: Main, Display,
Process Control, or Vacuum Filtration.
5. When downloading the Main firmware, the following dialog box appears:
6. Unplug the USB cable and plug it in again and click OK.
30 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
7. Select the instrument and click OK and the following dialog box appears:
11. When all of the required firmware modules have been downloaded, click
Close to close the dialog box.
12. If the firmware was successfully downloaded, DONE will appear in the
status field next to Firmware Download.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 31
4. XFlex Menu Options
32 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
3. The following dialog box appears for firmware versions higher than 2.05:
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 33
4. XFlex Menu Options
2. Click Set Offsets to write the default process control parameters to the
instrument.
3. Click Close to close the dialog box.
34 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 35
4. XFlex Menu Options
Active sensors will show the status Empty or Full as in the picture below
WARNING
3. Unscrew the lids from the liquid/waste bottles with active sensors and
move the float by hand to see if the sensors are working properly.
4. Click PASSED if all active sensors are working properly, otherwise click
FAILED.
5. Click Close to close the dialog box.
36 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
1. Select Low VAC Parameters from the Options menu to set the default
parameters for low vacuum.
2. Click Set Low VAC Parameters. The following message will apear if the
parameters have been set successfully:
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 37
4. XFlex Menu Options
2. Click Start Low VAC Test and the following message appears:
4. Check that the vacuum is holding the plate in place. Click Yes if the plate
is held firmly in place. The following message appears:
5. Check that the vacuum is not holding the plate in place. Click Yes if the
plate is not held in place.
6. When the test is complete, PASSED or FAILED appears.
7. Click Close to close the dialog box.
38 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 39
4. XFlex Menu Options
During the first step, the instrument builds up pressure and must reach
the target pressure within 2 minutes.
3. After the target pressure has been reached, place an empty microplate
(Greiner 96-well flat transparent) on the plate carrier.
5. Manually check that the vacuum pressure holds the plate in place on the
carrier. Click Yes or NO to start the third step of the test.
The following dialog box appears:
6. The vacuum pressure will be released. Check that the vacuum is NOT
holding the plate in place anymore. Click Yes or No to start the fourth
step of the test.
40 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
7. During the fourth step of the test, check that the vacuum pressure is
reduced from -850 mB to -100 mB within 2 minutes.
The following dialog box appears:
8. During the fifth and final step of the test, check that the vacuum pressure
is held at -100 mB, within specified limits, for 1 minute.
9. When the test is complete, PASSED or FAILED appears.
10. Click Close to close the dialog box.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 41
4. XFlex Menu Options
4.6.1 Y, Z Motors
1. Select Y, Z Motors from the Function Test menu to check the function of
the Y and Z motors.
42 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
5. Click Start Manifold Home Position Sensor Test to move the Z motor
to its home position. Click PASSED if the Z motor finds the home
position, otherwise click FAILED.
6. Under Manifold Head Up Sensor, click Start Sensor Test. Activate and
deactivate the sensor manually by lifting and lowering the manifold arm.
Click PASSED if the sensor state is displayed correctly, otherwise click
FAILED.
7. Click Close to close the dialog box.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 43
4. XFlex Menu Options
2. Click the Start button to start the test for the specified liquid channel.
The instrument will be primed using the selected channel.
3. Check the liquid flow and select PASSED or FAILED for the tested
channel.
4. Click Close after the liquid channels have been tested.
44 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2. Click the Prime button to start the priming procedure (use distilled water).
The following dialog box appears:
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 45
4. XFlex Menu Options
5. Make sure the channel 1 tube is in distilled water, click the Start Bubble
Sensor Test button. The following dialog box appears:
7. Pull the tube out of the distilled water and click OK to complete the test.
The procedure has PASSED if the acceptance criteria have been met,
otherwise the result is FAILED.
8. Click Close to close the dialog box.
46 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
4.6.6 Burn In
1. Select Burn In from the End Test menu to perform the endurance test.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 47
4. XFlex Menu Options
8-way manifold
A1
48 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
Note
If the manifold is moved too far in the Y or Z direction,
click Initialize Y/Z Motors and repeat the procedure.
10. Click the First Notch Found button to save this as the first position.
11. Move to the last position (notch) by clicking the Move to Last Notch
Default Position button.
12. Move the manifold to the edge of the last notch using the appropriate
Backward XXXX Step(s) buttons.
13. Lower the manifold using the appropriate Down XXXX Step(s) buttons
until the needles rest on the bottom of the notch.
14. Then lower the manifold again by 50 steps using the Down 50 Steps
button.
15. Then use the appropriate Backward XXXX Step(s) buttons to move the
manifold until it falls into the notch.
16. Click the Last Notch Found button to save this as the last position.
17. Save the newly calculated Y motor resolution by clicking the
Set Y Resolution button.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 49
4. XFlex Menu Options
2. Verify that a vacuum filtration plate carrier is not installed by clicking OK.
The following dialog box appears:
50 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 51
4. XFlex Menu Options
2. Click Calibrate Plate Sensor to start the procedure. Make sure that the
housing is mounted on the instrument to prevent false calibration results.
3. Insert a microplate and click Check Plate Sensor. The plate should be
detected: a green light and Plate will appear in the dialog box as shown
below:
4. Remove the microplate and click Check Plate Sensor again. The plate
should not be detected: a red light and No Plate will appear in the
dialog box.
5. Click PASSED if the sensor detects the plate correctly, otherwise click
FAILED.
52 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 53
4. XFlex Menu Options
2. Click the Prime button to start the priming procedure (use distilled water).
The following dialog box appears:
54 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
5. Make sure the channel 1 tube is in distilled water, click the Start Bubble
Sensor Calibration button. The following dialog box appears:
8. Pull the tube out of the distilled water and click OK to complete the
calibration. The procedure is complete and DONE appears.
9. Click Close to close the dialog box.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 55
4. XFlex Menu Options
2. Click the Prime button to start the priming procedure (use a solution of
distilled water with 0.1% Tween).
3. Weigh an empty microplate and enter its weight in the appropriate
Plate Weight field (mg). Place the plate on the plate carrier.
4. Dispense 50 l of distilled water into the plate by clicking the appropriate
Start Dispense button.
5. Weigh the microplate and enter its weight in the appropriate
Plate Weight field (mg). Place the plate on the plate carrier.
6. Dispense 250 l of distilled water into the plate by clicking the
appropriate Start Dispense button.
7. Weigh the microplate and enter its weight in the appropriate
Plate Weight field (mg). Place the plate on the plate carrier.
8. Click the Start Aspirate button to aspirate the liquid out of the wells.
9. Click the Calculate Calibration Factors button and the new calibration
factors are calculated and appear in the New Slope and Offset fields.
10. Click Write to Instrument to finish the dispense pump calibration
procedure.
11. The procedure is DONE if no errors occur, otherwise the result is
FAILED.
12. Click Close to close the dialog box.
56 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
(The Automatic Run 200 500 l/s button is available in versions > 2.05, except when working in Drip Mode)
2. Click the Prime button to start the priming procedure using channel 1
and a solution of 0.1% Tween.
3. Start each wash procedure by clicking the corresponding Start test: xxx
l/s button.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 57
4. XFlex Menu Options
4. Use a stopwatch to measure the dispense time. Enter the times in the
Time/Result fields.
5. Adjust the voltage value if necessary and click the corresponding Set
button.
6. Repeat the procedure for each speed until dispense time is within the
given range (Expected Time +/- 1 s).
7. For drip mode, check that the liquid is dripping and click OK if drip mode
is adjusted correctly. Dispense time is not measured for drip mode.
8. When the test is complete, click OK or FAILED.
9. The procedure has PASSED if the acceptance criteria have been met,
otherwise the result is FAILED.
58 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2. Click Check Plate Sensor Offset and the following dialog box appears:
3. Insert the strip-plate (Nunc 96-well plate with 8-well transparent strips)
and click OK.
After the first phase of the calibration procedure has finished, the
following dialog box appears:
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 59
4. XFlex Menu Options
60 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
4. Click PASSED if the manifold needles do not touch the edges of the
prime tub, otherwise the result is FAILED.
5. Click Close to close the dialog box after the test has finished.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 61
4. XFlex Menu Options
2. Click the Prime button to start the priming procedure (use a solution of
distilled water with 0.1% Tween on channel 1).
3. Weigh each of the 8 strips of a Greiner 96-well microplate (12-well
transparent strips) and enter each weight in the appropriate
Weight Strips X field (mg) under Weight Empty Strips.
4. Place the empty microplate on the plate carrier.
5. Dispense 200 l of distilled water/Tween solution into the plate by clicking
the Dispense 200 l button.
62 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2. Click the Prime button to start the priming procedure (use a solution of
distilled water with 0.1% Tween on channel 1).
3. Weigh each of the 8 strips of a Greiner 96-well microplate (12-well
transparent strips) and enter each weight in the appropriate
Weight Strips X field (mg) under Weight Empty Strips.
4. Place the empty microplate on the plate carrier.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 63
4. XFlex Menu Options
6. Click the Aspirate button to aspirate the liquid out of the wells.
Note
The aspiration test may fail if the Y/Z motor offsets have been incorrectly
set, thus leaving a greater residual volume after aspiration. See
4.8.1 Y/Z Motor Offsets for detailed information about setting the offsets.
Or the test may fail if the plate support is not level (see Leveling the
Plate Support in the technical manual).
64 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
2. Insert the adapter frame into the plate carrier. This is important to prevent
the wells from being affected by the magnets.
3. Click the Prime button to start the priming procedure (use a solution of
distilled water with 0.1% Tween on channel 1).
4. Weigh each strip of a Greiner 96-well microplate (12-well transparent
strips) and enter each weight in the appropriate Strip Weight X field (mg)
under Weight Empty Strips.
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 65
4. XFlex Menu Options
5. Place the empty microplate onto the adapter on the plate carrier.
7. Click the Aspirate button to aspirate the liquid out of the wells.
8. Weigh each strip again and enter each weight in the appropriate
Weight Strip X field (mg) under Weight Strips After Aspiration.
9. Click Calculate and the results will appear in the Result group box.
10. The procedure has PASSED if the acceptance criteria have been met,
otherwise the result is FAILED.
Note
The aspiration test may fail if the Y/Z motor offsets have been
incorrectly set, thus leaving a greater residual volume after aspiration
(see 4.8.1 Y/Z Motor Offsets for detailed information about setting the
offsets). Or the test may fail if the plate support is not level (see
Leveling the Plate Support in the technical manual).
66 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
4. XFlex Menu Options
4.9.1 Print
1. Select Print from the Print menu to print the results of the performed
tests. The following dialog box appears:
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4. XFlex Menu Options
68 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
Abbreviations
Abbreviations
The following abbreviations may appear in this IFU.
Abbreviation
C Celsius
CD Compact Disc
CE Conformit Europenne
cm Centimeter
CPU Central Processing Unit
DHR Device History Record
DT Dauertest (Endurance Test)
EN European Norm: a voluntary European standard of the
European Committee for Standardization or Comit
Europen de Normalisation (CEN)
ft Flat Transparent
FW Firmware
g Gram
GB Gigabyte
GHz Gigahertz
h Hour
HDD Hard Disk Drive
Hz Hertz
IEC International Electrotechnical Commission
IFU Instructions for Use
LLD Liquid Level Detection
mB/mBar Millibar
MB Megabyte
mg Milligram
ml Milliliter
mm:ss minutes:seconds
MTP Microtiterplatte (microplate)
No. Number
l Microliter
PC Process Control
RAM Read Accessible Memory
REF Reference Number/ Order Number
ROM Read Only Memory
s Second
SN Serial Number
TYPE Name and type of instrument
USB Universal Serial Bus
VAF Vacuum Filtration
WEEE Waste electrical and electronic equipment
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 69
Index
Index
Numbers refer to pages.
A I
About XFlex ................................................... 4-33 Info Pane ........................................................ 3-3
Adjust Menu .................................................. 4-23 Installation ...................................................... 2-2
Aspiration Pump ............................................ 4-19 Program ...................................................... 2-2
Instrument
B
Disinfection ................................................. 1-1
Bubble Sensor Calibration ............................ 4-26 Instrument Configuration ................................ 4-9
Bubble Sensor Test....................................... 4-21 Instrument Menu............................................. 4-2
Burn In ........................................................... 4-31 Instrument Settings......................................... 4-4
C L
Change User ................................................... 4-1 Language Download ...................................... 4-8
Channel Valve Test ....................................... 4-20 LLD Test ....................................................... 4-12
Connecting the HydroFlex Washer ................. 2-5 Log Entry ........................................................ 3-4
Control Pane ................................................... 3-3 Low VAC Parameters ................................... 4-13
Low VAC Test ............................................... 4-14
D
M
Disconnect/Connect ........................................ 4-2
Disinfection ...................................................... 1-1 Menu Description............................................ 3-2
Procedure .................................................... 1-2
O
Dispense Pump ............................................. 4-19
Dispense Pump Calibration .......................... 4-25 Options ........................................................... 4-1
Disposal Options Menu ............................................... 4-10
Operating Material ....................................... 1-3
P
E
Plate Sensor Offset ...................................... 4-24
End Test Menu ..............................................4-28 Prime .............................................................. 4-2
Print .............................................................. 4-32
F
Print Menu .................................................... 4-32
File Menu ........................................................ 4-1 Process Control Parameters ........................ 4-10
Firmware Download ........................................ 4-7 Process Control Test .................................... 4-11
Fixed Offsets ................................................... 4-9 Program
Function Test Menu ...................................... 4-18 Exiting ......................................................... 3-4
Uninstalling ................................................. 2-3
H
Q
Hardware Requirements ................................. 2-1
Header ............................................................ 3-2 Quit ................................................................. 4-1
Help Menu .....................................................4-33
High VAC Test .............................................. 4-15
2010-05 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 71
Index
R T
Requirements Tabs ................................................................ 3-3
Hardware..................................................... 2-1 Toolbar Description ......................................... 3-3
System ........................................................ 2-1
V
Rinse ............................................................... 4-3
Verify Aspiration Capability ........................... 4-30
S
Verify Dispense Capability ............................ 4-29
Save Plates and Programs ............................. 4-5 Verify Motor Positioning ................................ 4-28
Send Command .............................................. 4-3
Y
Serial Number ................................................. 4-6
Setup Menu .................................................... 4-6 Y, Z Motors .................................................... 4-18
Specifications.................................................. 2-1 Y/Z Motor Offsets .......................................... 4-23
Starting XFlex ................................................. 2-4
Status Bar Description .................................... 3-4
Store/Restore Menu ....................................... 4-4
System Requirements .................................... 2-1
72 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05
Tecan Customer Support
74 Instructions for Use for XFlex No. 30053297 Rev. No. 1.0 2010-05