198 Atherosclerosis Supplements 11, no.

2 (2010) 109222 Memory Stick Presentations

control group). Although there were no signicant differences (P >0.05) in MS438 CORONARY ARTERY ECTASIA: CLINICAL AND
both groups concerning mean age, gender, body mass index, antihypertensive ANGIOGRAPHICAL EVALUATION
pharmacotherapy and serum levels of high-density lipoprotein cholesterol and T. Aksu, B. Uygur, N. Arat, H. Ksack. Turkiye Yuksek Ihtisas Hospital, Ankara,
triglyceride, BP control was higher in the statin group (P = 0.016). Signicant Turkey
lower systolic BP (7.9 mmHg, P = 0.031) and diastolic BP (5.8 mmHg, Objectives: We investigated the prevalence, risk factors and distribution of
P = 0.006) levels were observed in the statin group. coronary artery ectasia (CAE) in patients undergoing coronary angiography for
Conclusions: These results indicate that statin therapy signicantly improves suspected coronary artery disease (CAD).
BP control in hypercholesterolemic hypertensive patients in clinical practice, Design: From 2004 to 2005, 12514 consecutive patients were submitted to
consistent with results obtained in some randomized clinical trials. These coronary angiography. Coronary angiograms were independently reviewed by
ndings might have useful implications for the prevention of cardiovascular two operators. Distribution of CAE was made according to the classication of
events in primary care patients. Markis and Ramappa.
Results: CAE was detected in 201 patients (1.6%) and was isolated in 30
MS436 SERUM hsCRP CONCENTRATION 2.0 mg/L IS ASSOCIATED patients (14.9%) and associated with aterosclerotic coronary artery disease
WITH CAROTID ATHEROSCLEROSIS, IN WOMEN WITH (ACAD) in 169 patients (84.1%). Among CAE patients, there was a marked male
VARYING DEGREES OF GLUCOSE TOLERANCE preponderance with 78.6%. Baseline features were similar between isolated
U. Prahl1 , J. Wikstrand2,3 , G. Bergstrom2,4 , B. Fagerberg1 , J. Hulthe2,3 . CAE and ACAD group.
1
Institute of Medicine, Department of Molecular and Clinical Medicine, The RCA was most commonly affected by CAE (45.3%). (Cx and LAD,
Wallenberg Laboratory for Cardiovascular and Metabolic Research, 39.3%, 31.5% respectively) According to the classication of Markis and
Sahlgrenska Academy, University of Got, 2 Wallenberg Laboratory for Ramappa, the majority of patients had type IV and type 4a ectasia. (77.6%,
Cardiovascular Research, and Center for Cardiovascular and Metabolic 57.7%respectively).
Research, Gothenburg, 3 AstraZeneca, Molndal, 4 Physiology Department,
Sahlgrenska University Hospital, Gothenburg, Sweden Table: Markis and Ramappa classication of CAE patients
Background: Rosuvastatin treatment of subjects with high-sensitivity CRP
Markis Class Ramappa Class Number of patients Percentage of
(hsCRP) 2.0 mg/L has been shown to powerfully reduce cardiovascular risk.
in patients in
We hypothesized that hsCRP 2.0 mg/L was associated with increases in
carotid intima media thickness (C-IMT), plaque burden and plaque echolucency Markis Ramappa Markis Ramappa
in the carotid arteries. Class Class Class Class
Material and Methods: A population sample of 64-year-old women (n = 635)
Type 1 Type 1a 7 3 3.5 1.5
with varying degrees of glucose tolerance underwent risk factor assessment
including measurement of hs-CRP and bilateral ultrasound examinations of the 1b 3 1.5
carotid arteries for measurement of IMT, plaque number and area, and plaque 1c 1 0.5
echogenicity. Type 2 Type 2a 24 15 11.9 7.5
Results: Subjects with hsCRP2.0 mg/L had elevated mean and maximum IMT 2b 9 4.5
in the carotid bulbs independently of other cardiovascular risk factors compared Type 3 Type 3 14 14 7.0 7.0
with those with hsCRP < 2.0 mg/L. There was no difference between the groups Type 4 Type 4a 156 116 77.6 57.7
in mean plaque area, although the subjects with plaques in the high hsCRP 4b 37 18.4
group had larger total plaque area than the subjects with plaque in the low
4c 3 1.5
hsCRP group. Plaque echolucency did not differ between the groups.
Conclusion: In this high risk female cohort hsCRP2.0 mg/L was accompanied
by elevated IMT in the carotid bulbs independently of other cardiovascular risk
factors. Total plaque area was greater among women with plaques in the high MS439 ASSOCIATION OF RS7138803 IN FAIM2 GENE AND RS7561317 IN
vs the low hsCRP subgroup. There was no difference in plaque echolucency TMEM18 GENE WITH ANTHROPOMETRIC VARIABLES IN A HIGH
between the two groups. CARDIOVASCULAR-RISK MEDITERRANEAN POPULATION
M. Sotos-Prieto, O. Portoles, C. Ortega-Azorin, P. Carrasco, M. Guillen,
MS437 STRONG UP-REGULATION OF RUNX2 IN DCC-SUSCEPTIBLE C. Saiz, D. Corella. Genetic and Molecular Epidemiology Unit, University of
C3H/HE MICE AFTER FREEZETHAW-INJURY Valencia, Valencia, Spain
A.-K. Sowa, J. Erdmann, H. Schunkert, Z. Aherrahrou. Department of Medicine
II, Universitat zu Lubeck, Lubeck, Germany Introduction: Abdominal obesity is associated with higher cardiovascular risk.
Several novel obesity genes have emerged from genome-wide association
Introduction: C3H/He mice were used as model for Dystrophic Cardiac studies (GWAs) as powerful candidates. Moreover, some of these genes have
Calcication (DCC) using freeze-thaw-injury. DCC shares many features with also been linked to the mechanisms regulating food intake. However, the
osteogenesis. associations are highly dependent of the population characteristics.
Aim: The aim of this study was to analyze the expression-level of transcription- Objective: To study the association between some polymorphisms in two
factors involved in osteogenesis and to identify respective target-genes of these relevant novel obesity genes, transmembrane protein 18 (TMEM18) and fas
for a better understanding of initiation and development of DCC. apoptotic inhibitor molecule 2 (FAIM2) with anthropometric variables and dietary
Methods: DCC-susceptible C3H/He and DCC-resistant C57BL/6 mice (n = 3) intake in a high-risk Mediterranean population.
were subjected to freeze-thaw-injury to induce calcication. Early at 24 and Methods: We included 945 subjects with high cardiovascular risk (average
72 hours necrotic and healthy myocardium from each mouse were separated. age: 676 years) participating in the PREDIMED (PREvencion con Dieta
tRNA and cryo-sections from each tissue were prepared for histological analysis MEDiterranea) Study, Valencia, Spain. Anthropometric, clinical, biochemical,
and relative-real-time-PCR using the DDCt -method. genetic and life-style data were obtained. We selected the rs7138803 (FAIM2)
Results: Using Calcein-staining calcication-like deposits appear in resistant and the rs7561317 as the most relevant for analysis.
and susceptible mice 1 day after injury. Calcication progresses in C3H/He Results: Minor allele frequencies (MAF) for the rs7138803 (FAIM2) and
but not in C57BL/6 mice 2 days later. Among the tested transcription-factors a rs7561317 (TMEM18) were A = 0.445 and A = 0.188, respectively. We found
30.26-fold up-regulation of Runx2 was detected in calcied tissue of C3H/He. a statistically signicant association between the rs7561317 in the TMEM18
Low expression was found for Sox9, Vdr, Nfkb, Msx1, Smad1, Smad2 and gene with waist circumference after multivariate adjustment (AA:99.812;
Smad4, none for Msx2, Twist1 and Smad3. Based on this nding we further AG:103.511.2; GG:104.812 cm; p = 0.042). Waist circumference and body
tested downstream-genes of Runx2: Vdr, Dmp1, Phex, Osterix, Col1a2, IBSP, weight were also higher in AA homozygous subjects for the rs7138803 in
MMP2, MMP8, MMP9, MMP13, Bglap II, Opn and Akp2. An up-regulation of FAIM2, without reaching the statistical signicance (p = 0.255 and p = 0.110
Col1a2 (4.45-fold of induction), of MMP8 (16.55-fold) and of MMP13 (15.17-fold) respectively). However, we found that AA subjects had higher energy intake
was observed. than individuals carrying the G allele (GA+GG) after adjustment for sex and
Conclusion: Inltrating cells differentiate into osteoblast-like-cells following age (AA 2107.5605.3 vs GA+GG 2261.3660.8 Kcal; p = 0.045).
injury through high expression of Runx2, which activates in turn the MMPs- Conclusion: The rs7561317 in TMEM18 was associated with waist
pathway to cleave collagen (type-I, -II, -III). The MMPs/collagen-interactions circumference and the rs7138803 in the FAIM2 gene with energy intake in
and their contribution in repair-processes and tissue-remodelling may explain this elderly Mediterranean population.
calcication in myocardium of susceptible mice.