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Ozonioterapia como terapia pr-oxidante:

Mecanismos de Ao e Evidncias Cientficas


O Homem radical livre
NIST, National Institute of Standards and Technology
Ozonioterapia
O que Oznio ?

o quarto oxidante mais potente na natureza


10 vezes mais solvel na gua que o oxignio
Altamente instvel, logo se recompe como oxignio
Oznio um radical livre ?

O oznio no uma molcula


radiclica porque possui um nmero O+
par de eltrons na rbita mais
externa (BOCCI, 2000), porm
muito oxidante e por isso reage O- O-
instantaneamente com outras
molculas podendo transform-las
em radicais livres (RL): ROIs, LOPs,
nutrientes....
Entra Sai

Oxignio Oznio
Medicinal Medicinal

SEMPRE
uma mistura
oxignio-oznio
Um bom
ozonioterapeuta com
um gerador de oznio
ineficiente produz m
Ozonioterapia...
O gs Oznio

ser inalado !!!


@draemiliaserra
*
@draemiliaserra
Reao com
antioxidantes plasmticos

Ativao de clulas
imunocompetentes e
plaquetas molculas de
sinalizao IF, IL, fatores de
crescimento

Ativao do metabolismo das hemcias -


2,3 difosfoglicerato e G6PDH
oxigenao tissular

Melhora das condies hemorreolgicas


Sangue
Corao
SNC ozonizado
Glndulas

Pulmes
Ossos

MALT
LOPs Msculos

Bao
TGI

Urina Rins
Fgado Bile
Pele
Os efeitos biolgicos imediatos do Oznio Medicinal
podem ser resumidos...

1- Ativao do metabolismo das hemcias

2 - Ativao das clulas imunocompetentes

3- Regulao do sistema antioxidante e radicais livres


Antioxidantes
3- Regulao do sistema antioxidante e radicais livres

Len, Bocci et al., 1998


Peralta et al., 1999

70 Protective effect of ozone in


60
Hepatic Ischaemia - Reperfusion
50
40
30 Ativao de enzimas anti-oxidantes e varredores
20
10 de radicais livres - nas clulas saudveis
0
SOD GSH
Kontrolle Ischaemia/Reperfusion Ozone+I/R

H2O2
https://www.ncbi.nlm.nih.gov/pubmed/17372841
Ozonioterapia
- dentro da janela teraputica -
induz stress oxidativo
leve, controlado e transitrio.
https://www.ncbi.nlm.nih.gov/pubmed/9071458
1995
https://www.ncbi.nlm.nih.gov/pubmed/9071458
2017
https://www.ncbi.nlm.nih.gov/pubmed/22583557
https://www.ncbi.nlm.nih.gov/pubmed/25699252
DIRETAMENTE DA TURQUIA

Ozonioterapia em recuperao de trauma raquimedular - em ratos: neste estudo, a


Ozonioterapia acelerou o processo de cicatrizao, aumentou a vascularizao e
reduziu a leso neuronal em roedores, sugerindo que a Ozonioterapia pode ser um
tratamento adjuvante em pacientes com leso raquimedular.

Turk Neurosurg. 2016 May 5.


doi: 10.5137/1019-5149.JTN.17508-16.1.

Effects of ozone on spinal cord recovery via Wnt/ -catenin pathway following
spinal cord injury in rats.
Tural Emon S1, Uslu S, Ilgaz Aydinlar E, Irban A, Ince U, Orakdogen M, Gulec-Suyen G.
https://www.ncbi.nlm.nih.gov/pubmed/27560537
Oznio e Preveno (Len et al. 2002 )
Baixas Doses

Insuflao retal de oznio em


a modelo animal
Depleo de glicognio em
clulas hepticas
a. Controle
b
b. Depleo de glicognio
induzida por CCl4

c c. Aplicao preventiva de
oznio como insuflao
retal (15 sesses)
https://www.ncbi.nlm.nih.gov/pubmed/20626256
https://www.ncbi.nlm.nih.gov/pubmed/22196863
https://www.ncbi.nlm.nih.gov/pubmed/19178770
CONCLUSION:
The results of this study suggests that major ozonated autohaemotherapy could be a safe and
effective therapeutic option for high-risk patients with dry AMD, and that a series of such
treatments could improve the natural course of AMD.
Effects of major ozonated autohemotherapy in the treatment of dry age related macular degeneration: a randomized controlled
clinical study.
Borrelli E1, Diadori A, Zalaffi A, Bocci V. Int J Ophthalmol. 2012;5(6):708-13. http://www.ncbi.nlm.nih.gov/pubmed/23275905
Artif Organs. 2017 Mar 5. doi: 10.1111/aor.12863.
[Epub ahead of print]

Ozone Prevents Cochlear Damage From Ischemia-Reperfusion Injury in Guinea Pigs.


Onal M1, Elsurer C2, Selimoglu N3, Yilmaz M4, Erdogan E4, Bengi Celik J5, Kal O6, Onal O5.

Author information
1Department of Otorhinolaryngology, Konya Educational and Training Hospital.
2Department of Otolaryngology, Selcuk University Medical Faculty.
3Department of Plastic Surgery, Konya Educational and Training Hospital.
4Department of Histology and Embryology.
5Department of Anesthesiology and Reanimation, Selcuk University Medical Faculty.
6Department of Nephrology, Baskent University Medical Faculty, Konya, Turkey.

Abstract
The cochlea is an end organ, which is metabolically dependent on a nutrient and oxygen supply to maintain its normal physiological
function. Cochlear ischemia and reperfusion (IR) injury is considered one of the most important causes of human idiopathic sudden
sensorineural hearing loss. The aim of the present study was to study the efficacy of ozone therapy against cochlear damage caused
by IR injury and to investigate the potential clinical use of this treatment for sudden deafness. Twenty-eight guinea pigs were
randomized into four groups. The sham group (S) (n = 7) was administered physiological saline intraperitoneally (i.p.) for 7 days. The
ozone group (O) (n = 7) was administered 1 mg/kg of ozone i.p. for 7 days. In the IR + O group (n = 7), 1 mg/kg of ozone was
administered i.p. for 7 days before IR injury. On the eighth day, the IR + O group was subjected to cochlear ischemia for 15 min by
occluding the bilateral vertebral artery and vein with a nontraumatic clamp and then reperfusion for 2 h. The IR group was subjected
to cochlear IR injury. After the IR procedure, the guinea pigs were sacrificed on the same day. In a general histological evaluation,
cochlear and spiral ganglionic tissues were examined with a light microscope, and apoptotic cells were counted by terminal
deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The apoptotic index (AI) was then calculated. Blood samples
were sent for analyses of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase, malondialdehyde (MDA), the total
oxidant score (TOS), and total antioxidant capacity (TAC). Data were evaluated statistically using the Kruskal-Wallis test. The AI was
highest in the IR group. The AI of the IR + O group was lower than that of the IR group. The biochemical antioxidant parameters SOD
and GSH-Px and the TAC values were highest in the O group and lowest in the IR group. The MDA level and TOS were highest in the IR
group and lowest in the O group. Controlled ozone administration stimulated endogenous antioxidant defense systems, thereby
helping the body to combat IR injury. Although this study revealed a statistically significant decrease in cochlear IR damage following
ozone therapy, further studies will be necessary to explain the protective mechanisms of ozone therapy in cochlear IR injury.

2017 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
https://www.ncbi.nlm.nih.gov/pubmed/28594115
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