A bbott Analytical

Consulting Scientists to the Disinfectant Industry

Certiicate of AnaLysis
Sample(s): One sample of Sterizar

Received from: Creative Supply Solutions Ltd. Malvern Mill, South Wing,
Earl Mill, Dowry St~eet, Oldham, OL8 2PF

Date received: 30 April 2010 Date tested: 14 May 2010

Certificate no: 100.140M20.CSS Certificate date: 17 May 2010

-~ Sample ref..:- 100/1-40 Page: 1 of 2

Analysis required: BS/EN 1650 quantitative suspension test for the evaluation
of chemical disinfectants (yeast & mould)

Product stored at: Room temperature

Active substance: Not declared

Test conditions: 'Dirty'

Product test concentration: 1:20 v/v

Product diluent used duri~g test: Sterile hard water 300mg/l CaC03

Contact time: 15 minutes

Test temperat~re:

Interfering substance: 3g/1 bovine albumin

Neutralising solution: 30g/1 polysorbate 80, 3g/1 lecithin,

19/1 histidine, 19/1 cysteine

I
Incubation temperature:

Ii Identification of fungal strains

used:
Aspergillus niger
Candida albicans
NCPF 2275
NCPF 3179

D C Watson

DC Watson BSc, CBio!, MlBiol, MIFST, ACTEHO

PO Box 95, New Ferry, Wirral, CH62 6HA
Tel: 07767 871275 Fax: 01516306540
email: abbottanalytical@hollllail.CO.uk:
 A bbott Analytical
Consulting Scientists to the Disinfectant Industry

17 May 2010 Certificate No: 10D.140MN.CSS Page 2 of 2

Test resu1.ts:

Organism Aspergi~~us Candida

ITest
niger aThicans
I 10 -1 Vel 106 Ve2 72 Vel 416 Ve2 368
~alidation
/Suspension NvO 8.90 xlO 2 NvO 3.92 xlO 3
I
"'--
iExperimental
~o -66-
.oeJ.......-M~C2 I vci, 322-1 VeL. 254-

IControl A. 7.50 xlO 1 A 2.88 xl02

I
I 100 Vel 76 Ve2 58 Vel 292 Ve2 346
!
INeutraliser
IControl B 6.70 xlO 1 B 3.19 xlO 2

100 Vel 56 Ve2 67 Vel 288 Ve2 362

kthOd
!validation C 6.15 x101 C 3.25 xl02

10 -6 Vel 29 Ve2 38 Vel 340 Ve2 374

10 -7 Vel 4 Ve2 7 Vel 42 Ve2 51

!Test
ISuspension N 4.43 x1G 7 N 4.11 x108

10 2 Vel 0 Ve2 0 Vel 0 Ve2 0

IResults
Na < 1.00 xlO 2 Na < 1.00 xlO 2

R > 4. 43 xlO 5 R >4.11 xlO 6

I .
,Log Reduction > 5. 65 > 6.61
I
I

Vc Viable count; N = efu/ml in the test suspension

Nv efu/ml in the validation suspension Na = efu/ml in the test mixture
R = Reduetion in viabili~y

Conc~usion:

This batch of Sterizar, when used neat, passes the requirements of EN 1650 for
fungicidal activity in 15 minutes at 20C under 'dirty' conditions against the
reference organisms detailed.

D C Watson

D C Watson BSc, CBiol, MIBiol, MIFST, ACIEHO

PO Box 95, New Ferry, Wirral, CH62 6HA
Tel: 07767 871275 Fax: 0151 6306540
email: abbottanalytica1@hotmail.co.uk