Table 4.1, Summary of operating characteristics and comparative costs of... https://www.ncbi.nlm.nih.gov/books/NBK143156/table/ch4.t1/?report=o...

Table 4.1 Summary of operating characteristics and comparative costs of dengue diagnostic
methods (9)

Diagnostic Diagnosis of Time to Specimen Time of Facilities Cost

methods acute results collection after
infection onset of
symptoms
Viral isolation and Confirmed 12 Whole blood, 15 days Mosquito or cell culture $$$
serotype weeks serum, tissues facilities, BSL-2/BSL-3a
identification laboratory, fluorescence
microscope or molecular
biology equipment

Nucleic acid Confirmed 1 or 2 Tissues, 15 days BSL-2 laboratory, equipment $$$

detection days whole blood, for molecular biology
serum,
plasma

Antigen detection Not yet 1 day Serum 16 days ELISA facilities $$

determined
Confirmed >1 day Tissue for NA Facilities for histology $$$
immuno-
chemistry

IgM ELISA Probable 12 days Serum, After 5 days ELISA facilities $

plasma,
IgM rapid test 30 whole blood No additional supplies
minutes

IgG (paired sera) Confirmed 7 days Serum, Acute sera, 15 ELISA facilities $
by ELISA, HI or or more plasma, days; BSL-2 laboratory
neutralization test whole blood convalescent after for neutralization assay
15 days

a Requirements may vary according to each country's national policies.

From: 4, LABORATORY DIAGNOSIS AND DIAGNOSTIC TESTS

Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control: New Edition.
Geneva: World Health Organization; 2009.

Copyright 2009, World Health Organization.

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