Aflatoxins are mold venom generated from Aspergillus Condition
flavus and about ten kinds of the related compounds are known. It is considered that Aflatoxins B1, B2, G1, G2 and Column: Finepak SIL-5 M1 can be carcinogens and contaminants for foods. Eluent: Toluen / Ethyl Acetate / MeOH / According to a hygienic method, five kinds of Aflatoxin Formic acid = 50 / 5 / 2 / 1 s ta n d a rd m i x tur e we r e s e p ar ated by n or m a l p ha s e Wavelength: Ex 365 nm, Em 425 nm, Gain x 100 chromatography and detected with a fluorescence detector. Flow rate: 1.0 mL/min. Figure 1 shows the chromatogram of Nuts pretreated Column temperature: 40C according to the procedure shown in Figure 2. Injection volume: 10 L Sample: STD mixture B1: 0.8 ppm B2: 0.4 ppm Keywords: 1. Aflatoxins, 2. STD mixture 3. Nuts G1: 0.4 ppm 4. SIL, 5. FL G2: 0.4 ppm M1: 0.6 ppm
Fig. 2 Pretreatment method
Fig. 1 Chromatogram of Aflatoxins standard mixture and Nuts
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