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catalase?
Its is known that the fruit and vegetables with the highest fat content
are the ones that contain the most amount of catalase, therefore this
test will provide data to support or contradict this fact.
VARIABLES
Dependent -Volume of oxygen foam released
Variables
Independent -The type of vegetables and fruits used
Variables
Controlled -Amount of hydrogen peroxide used
-Concentration of hydrogen peroxide used.
Variables
-Weight of sample of the fruit or vegetable used.
-Time given to allow the reaction to occur.
Apparatus
Method:
*Note: make sure that the sample comes from the meat of the
vegetable or fruit used, without skin. Exception will be made with
onion and cabbage when removing the skin. When using broccoli, only
use the trunk, do not use the the bush.
DATA COLLECTION
Table 1:
Graph:
Explanation: The vegetables that are closer to their root or have a big
seed have an optimum pH level, and since catalase produces better at
an optimum pH, the avocado (which contains a big seed) has the most
amount of catalase1
The apparatus used was the most precise available, although it was
not precise enough to give the exact results.
The experiment was carried in an 18 c room the first day and the
second day it was an 18 c room. This affects each result but it also
affects the results in overall since temperature is a know catalyst for
enzyme reactions.
Each time a new vegetable or fruit was going to be used the apparatus
was cleaned in order to assure that the sample being used was not
contaminated by the last sample that was used.
All the results are reliable since they were repeated 5 times and most
of the results are very accurate, except for the avocado and the bell
pepper which have already been explained.
This was a fair test since each of the samples were given the same
amount of hydrogen peroxide, with the same concentration and the
same time that would allow them to react.
1
http://www.chegg.com/homework-help/questions-and-answers/explain-vegetables-broccoli-
chives-garlic-capsicum-greater-catalase-activity-catalase-frui-q1352408