Escolar Documentos
Profissional Documentos
Cultura Documentos
PROGRAMME STRUCTURE
B.Tech BIOTECHNOLOGY (2016-17)
FIRST SEMESTER
Total 1000 23
1
SECOND SEMESTER
Industrial
3 ASH 204 2 0 0 20 20 40 60 100 2
Communication-II
Basic Electrical
EEL205/ Engineering/
4 3 1 0 20 20 40 60 100 4
EME206 Mechanical
Engineering
Fundamental of
Computer &
ECS207/ Programming/
5 3 1 0 20 20 40 60 100 4
EEC208 Fundamental of
Electronics
Engineering
Environmental
6 EBT 209 1 0 0 20 20 40 60 100 1
Studies
PRACTICALS
Basic Electrical
EEL225/ Engineering /
2 0 0 2 - - 40 60 100 1
EME 226 Mechanical
Engineering
Fundamental of
Computer and
3 FCP227 / Programming / 0 0 2 - - 40 60 100 1
EEC 228 Fundamental of
Electronics
Engineering
Workshop Practice /
EWS 210 /
4 Engineering 0 0 2 - - 40 60 100 1
EED 211
Drawing
Total 1000 23
2
THIRD SEMESTER
Total
Period Evaluation Scheme
Credits
Course
S. No Subject
Code
External Subject
Sessional Exam
Exam Total
L T P CA TA Total
Computer Based
Numerical and
5 EBT 305 3 1 0 20 20 40 60 100 4
Statistical
Techniques
Industrial
6 ASH304 2 0 0 20 20 40 60 100 2
Communication-III
PRACTICALS
Bio analytical
4 EBT 324 0 0 2 - - 40 60 100 1
Techniques Lab
Total 1000 26
3
FOURTH SEMESTER
Industrial 2 - - 20 20 40 60 100 2
5 ASH communication-IV
404
PRACTICALS
- - 2 - - 40 60 100 1
Food biotechnology
1 EBT 421
Lab
- - 2 - - 40 60 100 1
2 EBT 423 Cell & Tissue Culture
Techniques Lab
Total 800 22
4
FIFTH SEMESTER
L T P CA TA Total
Bioethics, Biosafety
1 EBT 501 3 1 0 20 20 40 60 100 4
& IPR
Enzymology &
2 EBT 502 Enzyme 3 1 0 20 20 40 60 100 4
Technology
Immunology &
3 EBT 503 3 1 0 20 20 40 60 100 4
Immuno technology
Bioprocess
4 EBT 504 3 1 0 20 20 40 60 100 4
Technology
Biomedical
5 EBT 505 3 1 0 20 20 40 60 100 4
Instrumentation
Industrial
6 ASH 504 2 0 0 20 20 40 60 100 2
Communication-V
PRACTICALS
Enzymology &
1 EBT 522 Enzyme Technology 0 0 2 - - 40 60 100 1
Lab
Immunology &
2 EBT 523 Immuno technology 0 0 2 - - 40 60 100 1
Lab
Bioprocess
3 EBT 524 0 0 2 - - 40 60 100 1
Technology Lab
Total 900 25
5
SIXTH SEMESTER
Downstream
1 EBT 601 3 1 0 20 20 40 60 100 4
Processing
Database
2 EBT 602 Management 3 1 0 20 20 40 60 100 4
Systems
Genetic
3 EBT 603 3 1 0 20 20 40 60 100 4
Engineering
Statistics for
5 EBT 605 3 1 0 20 20 40 60 100 4
Biology
Industrial
6 ASH604 2 0 0 20 20 40 60 100 2
Communication-VI
PRACTICALS
Downstream
1 EBT 621 0 0 2 - - 40 60 100 1
Processing lab
Total 900 25
6
SEVENTH SEMESTER
Health Care
Chemical
2 EBT 702 Engineering 3 1 0 20 20 40 60 100 4
Thermodynamics of
3 EBT 703 Biological 3 0 0 20 20 40 60 100 4
Systems
Current Topics in
4 EBT 704 3 1 0 20 20 40 60 100 4
Biotechnology
Industrial
5 ASH 705 3 1 0 20 20 40 60 100 2
Communication-VII
Total 700 22
7
EIGHTH SEMESTER
Entrepreneurship
Development
3 EBT 802 Programme 3 0 0 20 20 40 60 100 4
Marketing &
4 EBT 803 Management of 3 0 0 20 20 40 60 100 4
Biotechnology
Total 1100 22
Environmental biotechnology
8
ELEMENTARY MATHEMATICS-I
Unit 1:
The set theory and functions: Properties of subsets, linear and geometric functions, the binomial theories
of Integer, limits of functions (basic idea of limits of functions without analytical definitions), derivatives
of functions.
Unit 2:
Logarithm: Definition of laws of logarithm, use of logarithm table.
Unit 3:
Differentiation & Integration: General introduction.
Unit 4:
Algebra of Matrices: Definition of various types of matrices, symmetric and skew symmetric matrices,
Hermit Ian and skew Hermit Ian matrices, determinants up to order four, elementary properties of
determinants, product of determinants, inverse of a matrix, rank of matrix, linear equations.
Unit 5:
Introduction to Biostatistics: Sampling techniques, presentation of data, tabulation, frequency
distribution, graphical representation of data by Histogram, frequency polygon, frequency curve and
cumulative frequency curve.
Unit 6:
Measures of central tendency: Mean, Median & Mode.
Unit 7:
Measures of Dispersion: Mean deviation, standard deviation and standard error; variance concept,
calculation and theories of probability.
Unit 8:
Test of Significance: t- test, z-test, chi-square test and F-test.
References:
1 S.C Gupta & V.K Kapoor; Fundamentals of Statistics, Vol.1
2. Goon Gupta &Das Gupta: Fundamental of Statistics, Vo1,
3. Statistical methods in Biology by Norman Bailey
4. H. S. Bear: Understanding Calculus, John Wiley and Sons (Second Edition); 2003
9
ENGINEERING PHYSICS
Relativistic Mechanics: Inertial and Non-inertial Frames, Postulates of Special Theory of Relativity,
Lorentz Transformation, Length Contraction and Time Dilation, Addition of Velocities,
Mass Energy Equivalence and Variation of Mass with Velocity. Radiation: Kirchhoffs Law, Stefans
law (only statement), Energy spectrum of Blackbody Radiation.
UNIT-II
UNIT-III
Polarization: Phenomenon of Double Refraction, Ordinary and Extra-ordinary Rays, Nicol Prism,
Production and Analysis of Plane, Circularly and Elliptically Polarized Light, Optical Activity, Specific
Rotation, Polari meter. Laser: Principle of Laser Action, Einsteins Coefficients, Construction and
Working of He-Ne and Ruby Laser.
UNIT-IV
Electromagnetic: Amperes Law and Displacement Current, Maxwells Equations in Integral and
Differential Forms, Electromagnetic Wave Propagation in Free Space and Conducting Media, Pointing
Theorem. Phenomenon of Hysteresis and Its Applications
UNIT-V
Superconductivity:-
Essential properties of superconductors (zero resistivity), penetration depth and coherence length,
Meissner effect, critical field , Type I and Type II superconductors, applications of superconductors. Wave
Mechanics: Wave Particle Dualit y, de Broglie Concept of Matter Waves, Heisenberg Uncertainty
Principle, Schrdinger Wave Equation and Its Applications: Particle in a Box.
10
Reference Books:
1. Introduction to Special theory of Relativity Robert Resnick Wiley India
2. Physics of Atoms, Wehr Richards &Adia
3. Fundamentals of Physics, Halliday, Wiley India
4. Engineering Electromagnetics, WilliamHayt, 7t hEd,TMH
5. Ashutosh Asthana, Engg. Physics, BS Publication, Hyderabad
11
ENGINEERING CHEMISTRY
Molecular orbital theory, Molecular orbital diagram of diatomic molecules, hybridization, hydrogen
bonding, band theory of solids, liquid crystals with their classification applications, Braggs Law,
Fullerenes & their application , Nature of organic molecules, attacking reagents, inductive effect,
electromeric, mesomeric (resonance) effect, hyper conjugation, reaction intermediates types of
organic reaction (substitution, addition, elimination reaction &organic rearrangements), Saytzeffs
rule, organic name reactions (cannizzaros reaction, aldol condensation, Pinnacol- pinnacolon
rearrangement, Beckmanns rearrangement, Hoffmanns rearrangement), Optical isomerism &
confirmations, E-Z nomenclature, R-S configuration.
Rate of reaction, order & molecularity of reaction, Zero order, First Order, steady state
approximation, concept of activation energy, energy barrier, cell potential, liquid junction potential,
conductance & its variation with dilution, Transport no. Kohlraushs Law and its application, pH,
buffer solution, calculation of pH of buffer mixture solubility & solubility Product, Nernst distribution
law & its application, corrosion, its type, Mechanism & control , Theory of Electrochemical
corrosion. Hardness of water, boiler feed water, Softening of water (Calgon Process, Zeolite process,
Lime Soda process & Ion exchange process), Reverse osmosis, treatment of boiler feed water.
Introduction & classification of polymers, Types of Polymerization, bulk solution, suspension &
emulsion, copolymers, vulcanization, PVC, Polyamides, Polyurethane, Polyethylene, Poly propylene,
PET, Resins (Phenol Formaldehyde), PMMA, PAN, Rubber, Conducting and Biodegradable polymers,
Pyroceramics, Toughened glass, Strengthening of glass, Refractories, Nano Composites, Protective
Coatings, Fe, Al, Cu, Pb& Zn alloys, Organometallics & their applications.
Classification of Fuels, calorific value of fuel, gross & net calorific value, determination of calorific value
using Bomb calorimeter, Coal, Biomass and Biogas, Introduction of Lubricants, Mechanism of
Lubrication, Classification of Lubricant, Bio Lubricant, Flash and Fire Point, Pour Point, Cloud Point,
Aniline point, Viscosity index.
12
UNIT V ANALYTICAL METHODS AND APPLICATIONS
Titrimetric analysis with reference to acid-base, redox, precipitation and complex ometrictitrations.
Elementary ideas and simple applications of UV, visible, mass and NMR spectral techniques H NMR
spectral techniques.
REFERENCE BOOKS
1. Engineering Chemistry Wiley India
2. Engineering chemistry by Sivasankar, TMH, New Delhi.
3. Organic Chemistry by Morrisson& Boyd, Pearson Publication.
4. Organic Chemistry by Loudon, Oxford University Press.
5. C Parameswara Murthy, C V Agrawal and etal., Engineering Chemistry, BS
Publication, Hyderabad
13
INDUSTRIAL COMMUNICATION-I
Objective: The objective of the first semester course is to introduce the basics of communication language
and gradually develop a module to exhilarate the command over the language and its acquisition.
14
BASIC ELECTRICAL ENGINEERING
D.C. Network Theory: Circuit theory concepts-Mesh and node analysis. Network Theorems- Super-
position theorem. Thevenins theorem, Nortons theorem, Maximum, Power Transfer theorem,
Star Delta transformation.
Unit-2
Steady State Analysis of A.C. Circuits: Sinusoidal and phase or representation of voltage and current:
single phase A.C. circuit behavior of resistance, inductance and capacitance and their combination in
series & parallel and power factor, series parallel resonance-band width and quality factor
UNIT-3
Three Phase A.C. Circuits : Star-Delta connections, line and phase voltage/current relations, three
phase power and its measurement.
UNIT-4
Unit-5
Rotating Machine : Principle and construction of D.C. Machines ,Synchronous machine and induction
motor. Single phase Motors : Principle of operation and methods of starting of induction motor.Special
Machines: principle of operation and methods of starting of Stepper motor and Universal motor
Unit-6
References :
15
MECHANICAL ENGINEERING
UNIT-2 Law of Thermodynamics : Zeroth law: Zeroth law, Different temperature scales and
temperature measurement First law:First law of thermodynamics. Processes - flow and non-flow, Control
volume, Flow work and non-flow work, Steady flow energy equation, Unsteady flow systems and their
analysis. Second law: Limitations of first law of thermodynamics, Essence of second law, Thermal
reservoir, Heat engines. COP of heat pump and refrigerator. Statements of second law and their
equivalence, Carnot cycle, Carnot theorem, Thermodynamic temperature scale, Clausius inequality.
Concept of entropy.
UNIT-3 Properties of steam and Basic of IC engine: Properties of steam, Phase transformation process
and its graphical representation on P-V, T-V & T-s diagram, Mollier diagram and Steam Tables,
Processes involving steam in closed and open systems. Introduction to I.C. Engines: Two & four stoke
S.I. and C.I. engines. Otto cycle, Diesel cycle, Dual cycle.
UNIT-4 Force system and Analysis: Force system and Analysis Basic concept: Review of laws of
motion, transfer of force to parallel position, resultant of planer force system, Free Body Diagrams,
Equilibrium. Friction: Introduction, Laws of Coulomb friction, Equilibrium of bodies involving dry
fiction. Structure Analysis Beams: Introduction, Shear force and bending moment, Shear force and
bending moment diagram for statically determinate beams. Trusses: Introduction, Simple Trusses,
Determination of forces in simple truss members, Method of joints and Method of section.
UNIT-5 Stress and Strain Analysis : Simple stress and strain: Introduction, Normal shear stresses,
Stress-strain diagrams for ductile and brittle materials, Elastic constants, One dimensional loading
of members of varying cross section, Strain energy, Thermal stresses. Compound stress and strains:
Introduction, State of plane stress, Principal stress and strain, Mohrs circle for stress and strain. Pure
Bending of Beams: Introduction, Simple bending theory, Stress in beams of different cross sections. Torsion:
Introduction, Torsion of Shafts of circular section, Torque and Twist, Shear stress due to Torque.
Reference:
1. Agarwal, Basic Mechanical Engineering, Wiley India
2. Holman, J.P.: Thermodynamics, McGraw Hill book Co. NY.
3. Singh Onkar, Bhavikatti S.S., Chandra Suresh: Introduction to Mechanical Engineering:
Thermodynamics, Mechanics and Strength of Materials, New Age International Publishers
4. Yadav R.: Thermodynamics and Heat Engines, Vol I & II (SI Edition) Central Publ. House.
16
FUNDAMENTALS OF COMPUTER & PROGRAMMING
Unit 1
Basics of Computer System
Block Diagram of a Computer, Booting Process, Types of Computer Systems, Introduction to the
concepts: Bit, Byte, Word, hardware, Operating System and Application Software, Compilers,
Assemblers, Loaders and Linkers. ASCII& EBCDIC codes, Binary, Octal, Decimal & Hexadecimal
Number Systems & their Conversions
Unit 2
Features of DOS, Windows and UNIX Operating System and their Comparison, Concept of flowchart and
Algorithm, Machine, Assembly and high level languages, Structured Programming, Use of high level
programming languages for the systematic development of programs.
Unit 3
C Fundamentals
Character Set, Identifiers & Keywords, Data Types, Constants, Variables, Tokens, Operators &
Expression, Library Functions, Statements, Symbolic Constants, Preprocessor Directives, Functions.
Unit 4
Introduction, Classification of Array: One Dimensional Array, Multidimensional Array, Two Dimensional
Arrays. Defining a Structure, Array vs. Structure, Initialization of a Structure, Nested Structure
Unit 5
Pointers and File handling
Pointers Variables, Pointer Operator, Pointer Expression, Array of Pointers , Multiple Indirection,
Pointers to functions, dynamic allocation functions, File I/O: Streams and files, file System basic, fread,
fwrite ,fseeek, random access I/O, fprintf(), fscanf(), standard streams.
Reference Book
1. Let Us C: Yashwant Kanetkar [BPB]
2. Jones, C Programming with problem solving, Wiley India
3. Gupta: Computer Concepts & C Programming, Comdex
17
FUNDAMENTALS OF ELECTRONIC ENGINEERING
Unit 1: Semiconductor materials and properties :Group-IV materials, Covalent bond, electron-hole
concepts, Basic concepts of energy bands in materials, concepts of forbidden gap, Intrinsic and extrinsic
semiconductors, donors and acceptors impurities, Junction diodep-n junction, depletion layer, v- i
characteristics, dioderesistance, capacitance diode ratings ( average current, repetitive peak current,
non-repetitive current, peak-inverse voltage).
Unit-2: Diode Applications: Rectifiers (half wave and full wave), calculation of transformer utilization
factor and diode ratings, Introduction to filters, calculation of ripple factor and load regulation, clipping
circuits, clamping circuits, voltage multipliers Breakdowndiodes breakdown mechanisms (zener and
avalanche), breakdown characteristics, zener resistance, zener diode ratings, zener diode application as
shunt regulator
UNIT-3: Bipolar Junction Transistor : Basic construction, transistor action, CB, CE and CC
configurations, input/output Characteristics, concept of Biasing of transistors-fixed bias, emitter bias,
potential divider bias, Transistor Amplifier, Graphical analysis of CE amplifier, concept of voltage gain,
current gain, h-parameter model (low frequency), computation of Ai, Av, Ri, R O of single transistor CE
and CC amplifier configurations.
Unit-4: Field Effect Transistor : FET: Basic construction, transistor action, concept of pinch off,
maximum drain saturation current, input and transfer characteristics, characteristics equation CG, CS and
CD configurations, Introduction to self and fixed biasing .MOSFFT: depletion and enhancement type
MOSFET-construction, operation and characteristics. Computation of Av, Ri, Ro, of single FET
amplifiers using all the three configurations
Unit-5: Switching theory and logic design : Number systems, conversions of bases, Boolean algebra,
logic gates, concept of universal gate, concept of K- Map Operational Amplifiers: Concept of ideal
operational amplifiers, ideal op-amp parameters, inverting, non-inverting and unity gain amplifiers.
Reference Books:
18
ENVIRONMENTAL STUDIES
UNIT 1
NATURAL RESOURCES:
Renewable and Non-renewable Resources : Natural resources and associated problems.
a) Forest resources: Use and over-exploitation, deforestation, case studies. Timber extraction,
mining, dams and their effects on forest and tribal people.
b) Water resources: Use and over-utilization of surface and ground water, floods, drought, conflicts
over water, dams-benefits and problems.
c) Mineral resources: Use and exploitation, environmental effects of extracting and using mineral
resources, case studies.
d) Food resources : World food problems, changes caused by agriculture and over-grazing, effects of
modern agriculture, fertilizer-pesticide problems, water logging, salinit y, case studies.
e) Energy resources : Growing energy needs, renewable and non- renewable energy sources, use of
alternate energy sources. Case studies.
f) Land resources : Land as a resource, land degradation, man induced landslides, soil erosion and
desertification.
Role of an individual in conservation of natural resources.
Equitable use of resources for sustainable lifestyles.
UNIT 2
ECOSYSTEMS
Concept of an ecosystem.
Structure and function of an ecosystem.
Producers, consumers and decomposers.
Energy flow in the ecosystem.
19
Ecological succession.
Food chains, food webs and ecological pyramids.
b. Grassland ecosystem
c. Desert ecosystem
UNIT 3
BIODIVERSITY AND ITS CONSERVATION
Introduction Definition: genetic, species and ecosystem diversity.
Biogeographically classification of India
Value of biodiversity: consumptive use, productive use, social, ethical, aesthetic and option values
Biodiversity at global, National and local levels.
India as a mega-diversity nation
Hot-sports of biodiversity.
Threats to biodiversity : habitat loss, poaching of wildlife, man-wildlife conflicts.
Endangered and endemic species of India
Conservation of biodiversity: In-situ and Ex-sit conservation of biodiversity.
UNIT 4
ENVIRONMENTAL POLLUTION & SOCIAL ISSUES
Definition
m. Climate change, global warming, acid rain, ozone layer depletion, nuclear accidents and holocaust.
Case studies.
n. Wasteland reclamation.
o. Consumerism and waste products.
REFERENCE BOOK
1. MAjni Reddy, Text book of environmental Science, BS Publication, Hyderabad
2. Environmental Studies by Daniel, Wiley India
3. Environmental Studies by ErachBharucha, University Press.
4. Fundamental of Ecology, E.P.Odum, Cengage Learning.
5. Environmental Science and Engineering by Wright, Pearson Publication.6.
6. First Ecology by Beeby and Brennan, Oxford University Press.
7. Environment Science by Miller, Cengage Learning, New Delhi.
8. Hand book of Environmental laws, Rules, Guidelines, Compliances and Standards Vol. 1 & Vol. 2,
Bharat Publication, New Delhi.
21
PHYSICS PRACTICALS
2. To determine the wavelength of monochromatic light with the help of Fresnel s biprism.
3. To determine the focal length of two lenses by nodal slide and locate the position of cardinal points.
4. To determine the specific rotation of cane sugar solution using half shade polarimeter.
6. To determine the specific resistance of the material of given wire using Carey Fosters bridge.
7. To determine the variation of magnetic field along the axis of a current carrying coil and then to
estimate the radius of the coil.
10. To study the Hall Effect and determine Hall coefficient, carnier density and mobility of a given
semiconductor material using Hall-effect set up.
13. To draw hysteresis curve of a given sample of ferromagnetic material and from this to determine
magnetic susceptibility and permeability of the given specimen.
22
CHEMISTRY LAB
2. Determination of temporary and permanent hardness in water sample using EDTA as standard solution.
6. pH-metric titration.
7. Determination of Equivalent weight of Iron by the chemical displacement method. The equivalent
weight of copper is 63.5
(Note: the procedure to be followed in carrying the above experiment is given as annexure)
9. Determination of iron concentration in sample of water by colorimetric method. The method involves
the use of KCNS as color developing agent and the measurements are carried out at l max 480 nm.
Note: The general procedure of estimation is given on pp653-8 of the textbook of Quantitative Chemical
Analysis by A.I. Vogel 6th Edition, Publisher: Pearson education Ltd. 2000)
23
BASIC ELECTRICAL LAB
24
MECHANICAL ENGINEERING LAB
5. To conduct the tensile test on a UTM and determine ultimate Tensile strength, percentage elongation
for a steel specimen.
6. To conduct the compression test and determine the ultimate compressive strength for a specimen.
7. To conduct the Impact test (Izod / charpy) on the Impact testing machine and to find the impact
strength.
10. To determine the moment of inertia of a flywheel apparatus about its axis of rotation
12. To find out coefficient of friction by combined inclined plane & friction slide apparatus
13. To determine the velocity ratio, mechanical advantage & efficiency of a single purchase crab
apparatus & draw graph between load vs effort, mechanical advantage and efficiency.
14. To determine the velocity ratio, mechanical advantage & efficiency of a double purchase crab
apparatus.
25
FUNDAMENTALS OF COMPUTER PROGRAMMING LAB
List of Experiments
7. TO simulate calculator the perform the following operations on two numbers: Addition, subtraction,
Multiplication, division.
12. WAP to calculate the simple interest for a set of values representing principle, number of years and
rate of interest.
26
FUNDAMENTALS OF BASIC ELECTRONIC ENGINEERING LAB
3. Determination of ripple factor of capacitive and non -capacitive filter for HW and FW diode rectifier
circuit.
9. Study of BJT as single stage amplifier and determination of Ai, Vi, Ri and Ro.
27
WORKSHOP PRACTICE
1. Carpentry Shop: 1. Study of tools and operation and carpentry joints. 2. Simple exercise using jack
plain. 3. To prepare half- lap corner joint, mortise and tennon joints. 4. Simple exercise on woodworking
lathe.
2. Fitting Bench Working Shop: 1. Study of tools and operations 2. Simple exercises involving filling
work. 3. Making perfect male-female joint 4. Simple exercise involving drilling/tapping/dieing.
3. Black Smithy Shop: 1. Study of tools and operations. 2. Simple exercises based on black smithy
operations such as upsetting, drawing down, punching, bending, fullering& swaging.
4. Welding Shop: 1. Study of tools and operations . 2. Simple butt joint. 3. Lap joint. 4. oxy acetylene
welding.
5. Sheet metal shop: 1.Study of tools and operations. 2. Making funnel complete with soldering. 3.
Fabrication of tool box, tray, electrical panel box etc
6. Machine Shop: 1. Study of tools and operations. 2. Plane turning. 3. Step turning. 4. Taper turning 5.
Threading. 6. Single point cutting tool grinding.
Reference:
28
ENGINEERING DRAWING
2. Orthographic Projection
Principles of orthographic projections, Principal and auxiliary planes, First and Third angle projections.
Projection of points. Pictorial view. Projection of lines parallel to both the planes.
Parallel to one and inclined to other, Inclined to both the planes. Application to practical problems.
Projection of solid in simple position, Axis or slant edge inclined to one and parallel to other plane, Solids
lying on a face or generator on a plane.Sectioning of solids lying in various positions, True shape of the
section. Development of lateral surfaces, sheet metal drawing.
3. Isometric Projection Principles of isometric projection, Isometric projection using box and
offset methods.
Reference books:
29
ELEMENTARY MATHEMATICS-II
Unit-1
Probability Theory, Random Sampling, Binomial, Poisson, Normal Distributions. : mean, variance of
a distribution and probability of variables following these distributions.
Unit-II
Skewness, kurtosis of a distribution, moment generating function, Correlation: based on Measures of
central tendencies, Karl Pearson, Rank correlation , Regression: regression coefficient, & their properties.
Unit -III
Testing of Hypothesis: Concepts of Sampling, Statistics& Parameter, Types of errors, Level of
Significance, power of test, Critical region, Analysis of Variance (ANOVA):CRD, RBD designs, Problem
oriented approach to illustrate application of above Statistical methods
Unit-IV
Formulation of differential equations, solution of Ordinary differential Equations of 1st order , exact
differential equations of 1st order with constant coefficients: Complementary function and particular
integrals Solution of linear Partial Differential equations with constant coefficients & their classifications
into parabolic, elliptic and hyperbolic with illustrative examples.
References:
1. W. Danial : Biostatistics : A foundation for Analysis in Health Sciences, John Wiley and Sons
Inc; 2004.
2. A. Edmondson and D. Druce : Advanced Biology Statistics, Oxford University Press; 1996
3. Biostatistics:: A foundation for analysis in Health Science. 7 th Edition Wayne Daniel
4. Statistical methods in Biology by Norman Bailey
5. H. S. Bear: Understanding Calculus, John Wiley and Sons (Second Edition); 2003
30
ENGINEERING PHYSICS
Relativistic Mechanics: Inertial and Non-inertial Frames, Postulates of Special Theory of Relativity,
Lorentz Transformation, Length Contraction and Time Dilation, Addition of Velocities,
Mass Energy Equivalence and Variation of Mass with Velocity. Radiation: Kirchhoffs Law, Stefans
law (only statement), Energy spectrum of Blackbody Radiation.
UNIT-II
UNIT-III
Polarization: Phenomenon of Double Refraction, Ordinary and Extra-ordinary Rays, Nicol Prism,
Production and Analysis of Plane, Circularly and Elliptically Polarized Light, Optical Activity, Specific
Rotation, Polari meter. Laser: Principle of Laser Action, Einsteins Coefficients, Construction and
Working of He-Ne and Ruby Laser.
UNIT-IV
Electromagnetic: Amperes Law and Displacement Current, Maxwells Equations in Integral and
Differential Forms, Electromagnetic Wave Propagation in Free Space and Conducting Media, Pointing
Theorem. Phenomenon of Hysteresis and Its Applications
UNIT-V
Reference Books:
1. Introduction to Special theory of Relativity Robert Resnick Wiley India
2. Physics of Atoms, Wehr Richards &Adia
3. Fundamentals of Physics, Halliday, Wiley India
4. Engineering Electromagnetics, WilliamHayt, 7t h
5. Ed.(TMH)
6. AshutoshAsthana, Engg. Physics, BS Publication, Hyderabad
31
ENGINEERING CHEMISTRY
Molecular orbital theory, Molecular orbital diagram of diatomic molecules, hybridization, hydrogen
bonding,band theory of solids, liquid crystals with their classification applications, Braggs Law,
Fullerenes & their application , Nature of organic molecules, attacking reagents, inductive effect,
electromeric, mesomeric (resonance) effect, hyper conjugation, reaction intermediates types of
organic reaction (substitution, addition, elimination reaction &organic rearrangements), Saytzeffs
rule, organic name reactions (cannizzaros reaction, aldol condensation, Pinnacol-pinnacolon
rearrangement, Beckmanns rearrangement, Hoffmanns rearrangement), Optical isomerism &
confirmations, E-Z nomenclature, R-S configuration.
Rate of reaction, order &molecularity of reaction, Zero order, First Order, steady state approximation,
concept of activation energy, energy barrier, cell potential, liquid junction potential, conductance &
its variation with dilution, Transport no. Kohlraushs Law and its application, pH, buffer solution,
calculation of pH of buffer mixture solubilit y & solubility Product, Nernst distribution law & its
application, corrosion, its type, Mechanism & control , Theory of Electrochemical corrosion.
Hardness of water, boiler feed water, Softening of water (Calgon Process, Zeolite process, Lime Soda
process & Ion exchange process), Reverse osmosis, treatment of boiler feed water.
Introduction & classification of polymers, Types of Polymerization, bulk solution, suspension &
emulsion, copolymers, vulcanization, PVC, Polyamides, Polyurethane, Polyethylene, Poly propylene,
PET, Resins (Phenol Formaldehyde), PMMA, PAN, Rubber, Conducting and Biodegradable polymers,
Pyroceramics, Toughened glass, Strengthening of glass, Refractories, Nano Composites, Protective
Coatings, Fe, Al, Cu, Pb& Zn alloys, Organometallics & their applications.
Classification of Fuels, calorific value of fuel, gross & net calorific value, determination of calorific value
using Bomb calorimeter, Coal, Biomass and Biogas, Introduction of Lubricants, Mechanism of
Lubrication, Classification of Lubricant, Bio Lubricant, Flash and Fire Point, Pour Point, Cloud Point,
Aniline point, Viscosit y index.
32
UNIT V ANALYTICAL METHODS AND APPLICATIONS
Titrimetric analysis with reference to acid-base, redox, precipitation and complex metrictitrations.
Elementary ideas and simple applications of UV, visible, mass and NMR spectral techniques H NMR
spectral techniques.
REFERENCE BOOKS
1. Engineering Chemistry Wiley India
2. Engineering chemistry by Sivasankar, TMH, New Delhi.
3. Organic Chemistry by Morrisson& Boyd, Pearson Publication.
4. Organic Chemistry by Loudon, Oxford University Press.
5. C Parameswara Murthy, C V Agrawal and etal., Engineering Chemistry, BS
Publication, Hyderabad
33
INDUSTRIAL COMMUNICATION-II
Objective: Second module functions to enhance the ability of Reading skills and develop an attitude
towards language learning and comprehension skills.
34
BASIC ELECTRICAL ENGINEERING
D.C. Network Theory :Circuit theory concepts-Mesh and node analysis.Network Theorems- Super-
position theorem.Thevenins theorem, Nortons theorem, Maximum, Power Transfer theorem, Star
Delta transformation.
Unit-2
Steady State Analysis of A.C. Circuits: Sinusoidal and phase or representation of voltage and current:
single phase A.C. circuit behavior ofresistance, inductance and capacitance and their combination in series
& parallel and power factor, series parallel resonance-band width and quality factor
UNIT-3
Three Phase A.C. Circuits : Star-Delta connections, line and phase voltage/current relations, three
phase power and its measurement.
UNIT-4
Unit-5
Rotating Machine :Principle and construction of D.C. Machines ,Synchronous machine and induction
motor.Single phase Motors : Principle of operation and methods of starting of induction motor.Special
Machines: principle of operation and methods of starting of Stepper motor and Universal motor
Unit-6
Measuring Instruments: onstruction and principle of operation of voltage and current measuring
instruments; introduction to power and energy meters
References :
1. Dr. R. K. Singh and Dr. P.S. Subramanyam, Basic Electrical Engineering, BS
Publication, Hyderabad.
2. Gaikwad, Basic Electrical Engineering, Wiley India
3. A.e. Fitgerald, D.E., Higginbotham and A Grabel, Basic Electrical Engineering McGraw Hill.
4. H. Cotton, Advanced Electrical Technology Wheeler Publishing.
35
MECHANICAL ENGINEERING
UNIT-2: Law of Thermodynamics : Zeroth law: Zeroth law, Different temperature scales and
temperature measurement First law:First law of thermodynamics. Processes - flow and non-flow, Control
volume, Flow work and non-flow work, Steady flow energy equation, Unsteady flow systems and their
analysis. Second law: Limitations of first law of thermodynamics, Essence of second law, Thermal
reservoir, Heat engines. COP of heat pump and refrigerator. Statements of second law and their
equivalence, Carnot cycle, Carnot theorem, Thermodynamic temperature scale, Clausius inequality.
Concept of entropy.
UNIT-3: Properties of steam and Basic of IC engine: Properties of steam, Phase transformation process
and its graphical representation on P-V, T-V & T-s diagram, Mollier diagram and Steam Tables,
Processes involving steam in closed and open systems. Introduction to I.C. Engines: Two & four stoke
S.I. and C.I. engines. Otto cycle, Diesel cycle, Dual cycle.
UNIT-4: Force system and Analysis: Force system and Analysis Basic concept: Review of laws of
motion, transfer of force to parallel position, resultant of planer force system, Free Body Diagrams,
Equilibrium. Friction:Introduction, Laws of Coulomb friction, Equilibrium of bodies involving dry fiction.
Structure Analysis Beams: Introduction, Shear force and bending moment, Shear force and bending
moment diagram for statically determinate beams. Trusses: Introduction, Simple Trusses, Determination
of forces in simple truss members, Method of joints and Method of section.
UNIT-5: Stress and Strain Analysis :Simple stress and strain: Introduction, Normal shear stresses,
Stress-strain diagrams for ductile and brittle materials, Elastic constants, One dimensional loading of
members of varying cross section, Strain energy, Thermal stresses. Compound stress and strains:
Introduction, State of plane stress, Principal stress and strain, Mohrs circle for stress and strain. Pure
Bending of Beams: Introduction, Simple bending theory, Stress in beams of different cross
sections.Torsion: Introduction, Torsion of Shafts of circular section, Torque and Twist, Shear stress due to
Torque
Reference:
1. Agarwal, Basic Mechanical Engineering, Wiley India
2. Holman, J.P. : Thermodynamics, McGraw Hill book Co. NY.
3. Singh Onkar, Bhavikatti S.S., Chandra Suresh : Introduction to Mechanical Engineering:
Thermodynamics, Mechanics and Strength of Materials, New Age International Publishers
4. Yadav R. : Thermodynamics and Heat Engines, Vol I& II (SI Edition) Central Publ. House.
36
FUNDAMENTALS OF COMPUTER & PROGRAMMING
Unit 1
Basics of Computer System
Block Diagram of a Computer, Booting Process, Types of Computer Systems, Introduction to the
concepts: Bit, Byte, Word, hardware, Operating System and Application Software, Compilers,
Assemblers, Loaders and Linkers.ASCII& EBCDIC codes, Binary, Octal, Decimal & Hexadecimal
Number Systems & their Conversions
Unit 2
Introduction to Computer Operating System
Features of DOS, Windows and UNIX Operating System and their Comparison, Concept of Flowchart
and Algorithm, Machine, Assembly and high level languages, Structured Programming, Use of high level
programming languages for the systematic development of programs.
Unit 3
C Fundamentals
Character Set, Identifiers & Keywords, Data Types, Constants, Variables, Tokens, Operators &
Expression, Library Functions, Statements, Symbolic Constants, Preprocessor Directives, Functions.
Unit 4
Introduction, Classification of Array: One Dimensional Array, Multidimensional Array, Two Dimensional
Arrays.Defining a Structure, Array vs. Structure, Initialization of a Structure, Nested Structure
Unit 5
Pointers and File handling
Pointers Variables, Pointer Operator, Pointer Expression, Array of Pointers , Multiple Indirection,
Pointers to functions, dynamic allocation functions, File I/O: Streams and files, file System basic, fread,
fwrite ,fseeek, random access I/O, fprintf(), fscanf(), standard streams.
Reference Book
37
1 Let Us C :YashwantKanetkar [BPB]
1. Jones, C Programming with problem solving, Wiley India
2. Gupta: Computer Concepts & C Programming, Comdex
FUNDAMENTALS OF ELECTRONIC ENGINEERING
Unit 1 Semiconductor materials and properties :Group-IV materials, Covalent bond, electron-hole
concepts, Basic concepts of energy bands in materials, concepts of forbidden gap, Intrinsic and extrinsic
semiconductors, donors and acceptors impurities, Junction diode p-n junction, depletion layer, v- i
characteristics, dioderesistance, capacitance diode ratings ( average current, repetitive peak current,
non-repetitive current, peak-inverse voltage).
Unit-2 Diode Applications: Rectifiers (half wave and full wave), calculation of transformer utilization
factor and dioderatings, Introduction to filters, calculation of ripple factor and load regulation,clipping
circuits, clamping circuits, voltage multipliers
UNIT-3 Bipolar Junction Transistor : Basic construction, transistor action, CB, CE and CC
configurations, input/outputCharacteristics, concept of Biasing of transistors-fixed bias, emitter bias,
potential divider bias, Transistor Amplifier, Graphical analysis of CE amplifier, concept of voltage gain,
current gain, h-parameter model (low frequency), computation of Ai, Av, Ri, R O of single transistor CE
and CC amplifier configurations.
Unit-4 Field Effect Transistor : FET: Basic construction, transistor action, concept of pinch off,
maximum drain saturation current, input and transfer characteristics, characteristics equation CG, CS and
CD configurations, Introduction to self and fixed biasing .MOSFFT: depletion and enhancement type
MOSFET-construction, operation and characteristics. Computation of Av, Ri, Ro, of single FET
amplifiers using all the three configurations
Unit-5 Switching theory and logic design : Number systems, conversions of bases, Boolean algebra,
logic gates, concept of universal gate,concept of K- Map Operational Amplifiers: Concept of ideal
operational amplifiers, ideal op-amp parameters, inverting, non-inverting and unity gain amplifiers
Reference Books:
1.RobertBoylestad, Electronic Devices and Circuit theory, Pearsons Educations.
38
2. R. K. Singh &LalKishor, Basic Electronics Engineering, B S Publication, Hyderabad.
3. Gaikwad, Basic Electronics, Wiley India
4. A Mottershead, Electronic devices and circuits. PHI, 2000.
5. Morris Mano, Digital Computer Design, PHI, 2003.
ENVIRENVIRONMENTAL STUDIES
UNIT 1
NATURAL RESOURCES:
a) Forest resources : Use and over-exploitation, deforestation, case studies. Timber extraction,
mining, dams and their effects on forest and tribal people.
b) Water resources : Use and over-utilization of surface and ground water, floods, drought, conflicts
over water, dams-benefits and problems.
c) Mineral resources : Use and exploitation, environmental effects of extracting and using
mineral resources, case studies.
d) Food resources : World food problems, changes caused by agriculture and over-grazing, effects of
modern agriculture, fertilizer-pesticide problems, water logging, salinit y, case studies.
e) Energy resources : Growing energy needs, renewable and non renewable energy sources, use
of alternate energy sources. Case studies.
f) Land resources : Land as a resource, land degradation, man induced landslides, soil erosion
and desertification.
Role of an individual in conservation of natural resources.
Equitable use of resources for sustainable lifestyles.
39
UNIT 2
ECOSYSTEMS
Concept of an ecosystem.
Structure and function of an ecosystem.
Producers, consumers and decomposers.
Energy flow in the ecosystem.
Ecological succession.
Food chains, food webs and ecological pyramids.
Introduction, types, characteristic features, structure and function of the following ecosystems :-
a. Forest ecosystem
b. Grassland ecosystem
c. Desert ecosystem
d. Aquatic ecosystems (ponds, streams, lakes, rivers, oceans, estuaries)
UNIT 3
Threats to biodiversit y : habitat loss, poaching of wildlife, man-wildlife conflicts. Endangered and
endemic species of India
Conservation of biodiversit y : In-situ and Ex-situconservation of biodiversity.
UNIT 4
REFERENCE BOOK
1. MAjni Reddy, Text book of environmental Science, BS Publication, Hyderabad
2. Environmental Studies by Daniel, Wiley India
3. Environmental Studies by ErachBharucha, University Press.
4. Fundamental of Ecology, E.P.Odum, Cengage Learning.
5. Environmental Science and Engineering by Wright, Pearson Publication.6.
6. First Ecology by Beeby and Brennan, Oxford University Press.
41
7. Environment Science by Miller, Cengage Learning, New Delhi.
8. Hand book of Environmental laws, Rules, Guidelines, Compliances and Standards Vol. 1 & Vol. 2,
Bharat Publication, New Delhi.
42
INDUSTRIAL COMMUNICATION-II
Objective: Second module functions to enhance the ability of Reading skills and develop an attitude
towards language learning and comprehension skills.
43
PHYSICS PRACTICALS
3. To determine the focal length of two lenses by nodal slide and locate the position of cardinal points.
4. To determine the specific rotation of cane sugar solution using half shade polarimeter.
6. To determine the specific resistance of the material of given wire using Carey Fosters bridge.
7. To determine the variation of magnetic field along the axis of a current carrying coil and then to
estimate the radius of the coil.
10. To study the Hall Effect and determine Hall coefficient, carnierd ensityand mobility of a given
semiconductor material using Hall-effect set up.
13. To draw hysteresis curve of a given sample of ferromagnetic material and from this to determine
magnetic susceptibility and permeability of the given specimen.
44
CHEMISTRY LAB
2. Determination of temporary and permanent hardness in water sampleusing EDTA asstandard solution.
6. pH-metric titration.
7. Determination of Equivalent weight of Iron by the chemical displacement method. The equivalent
weight of copper is 63.5
(Note : the procedure to be followed in carrying the above experiment is given as annexure)
9. Determination of iron concentration in sample of water by colorimetric method. The method involves
the use of KCNS as colour develop ingagent and the measurements are carried out at l max 480 nm.
Note : The general procedure of estimation is given on pp653-8 of thetextbook of Quantitative Chemical
Analysis by A.I. Vogel 6th Edition,Publisher : Pearson education Ltd. 2000)
45
BASIC ELECTRICAL LAB
46
MECHANICAL ENGINEERING LAB
5. To conduct the tensile test on a UTM and determine ultimate Tensile strength, percentage elongation
for a steel specimen.
6. To conduct the compression test and determine the ultimate compressive strength for a specimen.
7. To conduct the Impact test (Izod / charpy) on the Impact testing machine and to find the impact
strength.
10. To determine the moment of inertia of a flywheel apparatus about its axis of rotation
12. To find out coefficient of friction by combined inclined plane & friction slide apparatus
13. To determine the velocity ratio, mechanical advantage & efficiency of a single purchase crab
apparatus & draw graph between load vs effort, mechanical advantage and efficiency.
14. To determine the velocity ratio, mechanical advantage & efficiency of a double purchase crab
apparatus.
47
FUNDAMENTALS OF COMPUTER PROGRAMMING LAB
List of Practical
7. TO simulate calculator the perform the following operations on two numbers: Addition, subtraction,
Multiplication, division.
12. WAP to calculate the simple interest for a set of values representing principle, number of years
and rate of interest.
48
FUNDAMENTALS OF BASIC ELECTRONIC ENGINEERING LAB
3. Determination of ripple factor of capacitive and non capacitive filter for HWand FW diode
rectifier circuit.
9. Study of BJT as single stage amplifier and determination of Ai, Vi, Ri and Ro.
49
WORKSHOP PRACTICE
1. Carpentry Shop: 1. Study of tools and operation and carpentry joints. 2. Simple exercise
using jack plain. 3. To prepare half- lap corner joint, mortise and tennon joints. 4. Simple
exercise on woodworking lathe.
2. Fitting Bench Working Shop : 1. Study of tools and operations 2. Simple exercises
involving filling work. 3. Making perfect male-female joint 4. Simple exercise involving
drilling/tapping/dieing.
3. Black Smithy Shop: 1. Study of tools and operations 2. Simple exercises based on black smithy
operations such as upsetting, drawing down, punching, bending, fullering& swaging.
4. Welding Shop: 1. Study of tools and operations . 2. Simple butt joint. 3. Lap joint. 4.
oxy acetylene welding.
5. Sheet metal shop: 1.Study of tools and operations. 2. Making funnel complete with soldering.
3. Fabrication of tool box, tray, electrical panel box etc.
6. Machine Shop: 1. Study of tools and operations. 2. Plane turning. 3. Step turning. 4. Taper
turning 5. Threading. 6. Single point cutting tool grinding.
Reference:
50
ENGINEERING DRAWING
3. Isometric Projection Principles of isometric projection, Isometric projection using box and offset
methods.
Reference books:
51
CELL BIOLOGY
Unit I
Cell: An introduction, classification of organisms by cell structure, cytosol, compartmentalization of
eukaryotic cells, cell fractionation.
Unit II
Cell membrane and permeability: Chemical components of biological membranes, organization and
fluidity of membrane components, the membrane as a dynamic entity, cell recognition and membrane
transport.
Unit III
Organization of transport activity in cell. Signal Tans duction. Cell signaling: Types of signaling,Cell
surface receptor mediated signaling (RTK, pathway, JAK-STATpathway), G-proteins and G-protein
coupled receptors, Secondary messengers and intracellular communication, Target cell adaptation.
Unit IV
Cytoskeleton and cell motility: Structure and functions of microtubules, microfilaments, intermediate
filaments. Structure and Functions of Cellular Organelles: Endoplasmic reticulum, golgi complex,
lysosomes, vacuoles and microbodies, ribosomes, mitochondria, plastids.
Unit V
Nucleus: Structure, cell-cycle (interphase and M phases), regulation of cell cycle.
Extra cellular matrix: Composition, molecules that mediate cell adhesion, membrane receptors
for extra cellular matrix macromolecules, regulation of receptor expression and function
Text/ References Books:
th
1. Molecular Biology of cell, 4 ed.Alberts,Bruce (etal)(2002) Garland Science Publishing, New
York.
2. Cell Biology-Smith and Wood by Chapmanand Hall.
3. Cell Biology: Organelle structure and function, Sadava,
DE.(2004) Panimapub., NewDelhi.
th
4. Cell and Molecular Biology, 8 ed.Robertis, EdpDeandRobertis, EmfDe(2002) Lippincot
Williams and Wilkins Pvt.Ltd., (International Student Edition) Philadelphia.
th
5. Molecular Cell Biology 4 ed.Lodish, Harvey and Baltimore, D (2000)W.H.freeman & Co.
New York
52
BIOCHEMISTRY-I
Unit I
Hydrogen bonding and structure of water molecule, lionization of water, pH, Buffers. Colligative
properties of water. Carbohydrates-Structure and functions: Structures and properties of mono
saccharides, oligosaccharides and polysaccharides. Ring structure and mutarotation. Homo - and
hetero- polysaccharides. Mucopolysaccharides.
Unit II
Amino acids & Proteins: Structure and properties of amino acids. Essential and non-essential amino
acids. Peptide bonds. Types of proteins and their classification. Forces stabilizing protein structure and
shape. Different levels of structural organization of proteins. Lipids-Structure and functions:
Classification of lipids and their general functions. Essential fatty acids. Hydrolysis of fats,
Saponification value, Rancidity of fats, Cholesterol- its structure and biological functions.
Unit III
Nucleic Acids-Structure and functions: Structure and properties of purine and pyrimidinebases.
Nucleosides and nucleotides. Biologically important nucleotides. Vitamins: Role of Vitamins, metals
ions, significance.
Unit IV
Metabolism: Basicconcepts, Anabolism and catabolism, Carbohydrate Metabolism: Glycolysis. Fate of
pyruvate under aerobic and anaerobic conditions. Pentose phosphate pathway and its significance.
Gluconeogenesis pathway. Maintenance of blood glucose level. Energetics and regulation of
carbohydrate metabolism. Lipid Metabolism: Beta-oxidation of saturated fatty acids, oxidation of
unsaturated and odd carbon fattyacids. Formation and utilization of ketone bodies.
Unit V
Amino Acid Metabolism: General reactions of amino acids metabolism-transamination, oxidative and
non-oxidative deamination and decarboxylation. Urea cycle and its regulations. Nitrogencycle.
Nucleic Acid Metabolism: Catabolism, de novo-biosynthesis and regulation of purine and pyrimidine
nucleotides. Formation of deoxy ribbo nucleotides.
Recommended Books:
1. Principles of Biochemistry: A.L. Lehninger, Nelsonand Cox, McMillan Worth Publishers.
2. Biochemistry: Voetand Voet, John Wiley and Sons, Inc. USA.
3. Biophysical Chemistry Vol.I,II&III: Cantorand Schimel, Freeman.
53
4. Biochemistry: Zubey, WCB.
54
MICROBIOLOGY
Unit 1
Development of microbiology, scope and relevance of microbiology, Fieldsin microbiology, History of
Microbiology: Contribution of Koch, Contribution of Lister, germ theory of fermentation, Conflicts of
Biogenesis and Abiogenesis, Contribution of Met kinik offs.
ClassificationofMicroorganism,TwoKingdomclassification,Threekingdomclassification,Five Kingdom
classification,Eightkingdomclassification.DifferencebetweenEukaryoteand Procaryote, method of
classification based on 16sRNA, DNA homology, Identification of BacteriaBiochemicaland molecular
Approach.
Unit II
Morphology and ultra structureofbacteria.Morphologicaltypes.Cellwallofarchaebacteria, Gram,
negative and Gram-positivebacteria,eukaryotes.L-formcellwallsynthesis.Capsules- types composition
and function. Cell membranes- structure, composition and properties. Cultivation of aerobic and
anaerobic bacteria.
Unit III
Structure and function of flagella, cilia, pili, gas vesicles, chromosomes, carboxysomes,
magnetosomesandphycobilisomes, nucleoid, cell division. Spores, reservefood materials
polyhydroxybutyrate, phosphate granules. Oil droplets, cyanophycingranulesand sulfur inclusions.
Unit IV
Cultivation of bacteriaaerobic, anaerobic, nutritional types, culture media used, growth curve
generation time. Growth kinetics, asynchronous and synchronous growth Batchandcontinuous cultures.
Measurement of growth, factors affecting growth. Control of bacteria-
physicalandchemicalagents.IsolationofPureculture,Preservation methods. Sterilising techniques,
Unit V
Virology: Structure and cultivation of Virus, General, Brief outline on discovery of viruses.and. Types
of envelopes and their compositions. Viral genome, their types structures,virusrelatedagents (viroids
virusoids and prions).Bacteriophage and its lifecycle.
Recommended Books
55
BASIC BIO ANALYTICAL TECHNIQUES
Unit I:
Introduction
Types of Analytical Methods, Instrument for analysis, Uncertainties in Instrumental measurements
Sensitivity and detection limit for instruments, pH meter, dissolved oxygen, agitation, sensors and their
operation.
HPLC, Gas Chromatography, Ion Exchange Chromatography, Gel Filtration Chromatography, Affinity
Chromatography, Membrane separation, Ultrafilteration, Reverse Osmosis.
Text Books
1. AbiologistGuidetoprinciplesandtechniqueofpracticalbiochemistry-ByKeithWilson, Kenneth
rd
H. Gouldind3 Edition,ELBS Series
2. SkoogandWest, Fundamentals of Analytical Chemistry, 1982.
3. Vogel, Text Book ofQuantitativeInorganic analysis, 1990.
4. Ewing,Instrumental Method of analysis, 1992
5. HobertHWillard,D.L.MerrittandJ.R.J.A.Dean,instrumentalmethodsofanalysis,CBS Publishers
and Distributors, 1992
6. F.Settle.HandBookofInstrumentalTechniquesforAnalyticalchemistry,PrenticeHall,
1997.
56
COMPUTERBASED NUMERICAL ANDSTATISTICAL TECHNIQUES
Unit-I
Floating point Arithmetic: Representation of floating point numbers, Operations, Normalization, Pitfalls of
floating point representation, Errors in numerical computation. Iterative Methods: Zerosofa single
transcendentalequationandzerosofpolynomialusingBisectionMethod,Iteration Method, RegulaFalsi method,
Newton Raphson method,Secant method, Rate of convergence of iterative methods.
Unit-II
Simultaneous Linear Equations: Solutions of system of Linear equations, Gauss Elimination directmethod and
pivoting ,Ill Conditioned system of equations, Refinement of solution.Gauss Seida literative method, Rate of
Convergence. Interpolation and approximation: Finite Differences, Difference tables, Polynomial Inter
polation: Newtons forward and backward formula,Central Difference Formulae: Gauss forward and backward
formula, Stirlings, Bessels, Everettsformula.
Interpolation with unequalintervals: Langranges Inter polation, Newton Divided difference formula,
HermitesInterpolation,Approximationoffunctionby Taylors series and Chebyshev polynomial
Unit-III
Numerical Differentiation and Integration:
Introduction, Numerical Differentiation, Numerical Integration, Trapezoidal rule, Simpsons rules, Booles
Rule, Weddles RuleEulerMaclaurin Formula.
Solution of differential equations:
Picards Method, Eulers Method, Taylors Method, RungeKutta methods, Predictorcorrector method,
Automatic error monitoring, stability of solution.
Unit-IV
Curvefitting, Cubic Spline and Approximation:Method of least squares,fittingofstraight lines, polynomials,
exponential curvesetc. Frequency Chart: Different frequencychart like Histogram,Frequencycurve, Pichart.
Regression analysis: Linear and Nonlinearregression, multiple regressions.
References:
1. RajaramanV.,ComputerOrientedNumerical Methods, PHI
2. Gerald&Wheatley, Applied Numerical Analyses, AW
3. Jain, Iyengar and Jain, Numerical Methods forScientific and EngineeringComputations, NewAgeInt.
4. GrewalB. S., Numerical methods in Engineeringand Science, KhannaPublishers, Delhi
5. T. Veerarajan, T Ramachandran,TheoryandProblems in Numerical Methods, TMH
6. PradipNiyogi, Numerical Analysis and Algorithms, TMH
57
Industrial Communication-III
Course Code: ASH 304 Credits:02
Objective: Third Module improves the writing ability to produce Technical and nontechnical documents
Unit-1: Learning Writing Skills
Definition and Introduction
Importance of Writing Skills
Types of Writing of Skills
Techniques of Writing Skills
Objectives of Improving Writing Skills
Methods of improving Writing Skills
58
CELL BIOLOGY
Course Code: EBT 321 Course Credits:01
List of experiments
Module II: Micrometer (To measure the length and breadth of the given cell sample by using micrometer.).
Module III: To prepare a temporary stained mount of onion peel to see the structure of cells under the
microscope.
Module IV: Permanent slide preparation (To prepare permanent slides using the given sections like Stem,
Module V: To identify the different types of cells present in the leaf cross section.
Module VI: Preparation of wax embedded slides and tissue sectioning with the help of microtome.
Module VII: Cell Counting and viability (To identify the number of cells present in the given 1ml sample).
59
BIOCHEMISTRY LAB
Course Code: EBT 322 Course Credits:01
List of experiments
60
MICRO BIOLOGY LAB
Module II: Isolation and maintenance of organisms by plating, streaking and serial dilution.
Module VI: Effect of temperature, pH, carbon and nitrogen sources on growth of bacteria.
61
BIOANALYTICAL LAB PRACTICALS
List of experiments
Module I: To study the working and principle of instrument used in the Basic bio analytical technique lab.
62
FOOD BIOTECHNOLOGY
Unit: 1
Unit: 2
Microorganisms in fresh meats and poultry, processed meats, seafoods, fermented and fermented dairy
products and miscellaneous food products. Starter cultures, cheeses, beer, wine and distilled spirits, SCP,
medical foods, probiotics and health benefits of fermented milk and foods products. Brewing, malting,
mashing, hops, primary & secondary fermentation: Biotechnological improvements: catabolic repression,
High gravity brewing, B-glucan problem, getting rid of diacetyl. Beer, wine and distilled spirits.
Unit: 3
Nutritional boosts and flavor enhancers: Emerging processing and preservation technologies for milk and
dairy products. Microbiological Examination of surfaces, Air Sampling, Metabolically Injured
Organisms.Enumeration and Detection of Food-borne Organisms. Bioassay and related Methods
Unit: 4
Food Preservation, Food Preservation Using Irradiation, Characteristics of Radiations of Interest in Food
Preservation. Principles Underlying the Destruction of Microorganisms by Irradiation, Processing of Foods
for Irradiation, Application of Radiation, Radappertization, Radicidation, and Radurization of Foods Legal
Status of Food Irradiation, Effect of Irradiation of Food constituents.
Unit: 5
Storage Stability Food Preservation with Low Temperatures, Food Preservation with High Temperatures,
Preservation of Foods by Drying, Indicator and Food-borne Pathogens, Other Proven and Suspected Food-
borne Pathogens. Rheology of Food Production.
1. Frazier, W.S. and Weshoff, D.C., 1988. Food Microbiology, 4th Edn., McGraw Hill Book Co.,
New York.
2. Mann &Trusswell , 2007. Essentials of human nutrition. 3rd edition .oxford university press. 3. Jay,
J.M., 1987. Modern Food Microbiology, CBS Publications, New Delhi. 4 Lindsay, 1988. Applied
Science Biotechnology. Challenges for the flavour and Food Industry. Willis Elsevier. 5. Roger,
A., Gordon, B. and John, T., 1989. Food Biotechnology.
63
GENETICS
Course Objective:
The objective of the course is to focus on the basic principles of genetics incorporating the concepts of
classical, molecular and population genetics. Compilation is required for recent advances in genetic
principles for strong foundation in Biotechnology.
Course Contents:
Module I
The science of genetics -introduction, history, classical and molecular genetics, role of genetics in
medicine, agriculture and society.
Module II
Mendelian law of inheritance and its applications, Extension of Mendelism - Allelic variations, influence of
environment on expression, penetrance and expressivity, epistasis, pleiotropy. Pedigree analysis;
Chromosomal basis of inheritance; sex linkage,, crossing over and chromosome mapping in eukaryotes.
Module III
Genetic code and protein biosynthesis; Gene regulation, Genes in development; Extra chromosomal
inheritance, Male sterility and incompatibility; Recombination in bacteria, fungi and viruses, tetrad analysis.
Module IV
Numerical changes and structural changes in chromosomes with emphasis on human disease/syndromes.
Module V
Mutation and mutagenic agents, types of mutations; Transposons: types of transposons; Concept of gene
classical and modern, psendoallelism, position effect, intragenic crossing over & complementation.
Module VI
Population genetics; Genetic drift, Hardy- Weinburg Law and its deviations.
References:
1. Genetics, M.W. Strickberger, Prentice Hall College Division
64
CELL & TISSUE CULTURE TECHNIQUES
Unit 1: Introduction to animal tissue culture: Background, advantages, limitations, application, major
differences in vitro, types of tissue culture, Initiation of the culture: Culture environment, Origin and
evolution of cell lines, cell proliferation, cell adhesion, cell differentiation and micro-environment. Basic
techniques of cell cultures: Primary and secondary cell culture, Growth factors used in culture,
Unit 2: Cell lines: Preparation of medium, nomenclature, subculture and propagation, immortalization of
cell lines, designations, routine maintenance, mass culture techniques. Characterization and quantification
of Cell line: Direct and indirect methods for quantification, characterization of cultured cells, morphology,
parameter of growth, phases of cell growth, multiplication rate, application of animal cell culture and stem
cell culture.
Preservation and contamination: Need of cryopreservation, source of contamination, type of microbial
contamination, monitoring, eradication of contamination and cross-contamination. Cell Banking, Gene
transfer and transfection procedures.
Unit 4: Classical vs. modern approach of Plant tissue Culture. Plasticity and totipotency, plant cell culture
media, plant growth regulators. Protoplast Related Techniques Protoplast, Isolation, Culture and fusion,
Selection of hybrid cells, regeneration of hybrid plants, somatic hybridization and cybridization,
Applications in crop improvement. Plant regeneration: somatic embryogenesis and organogenesis. Culture
types, callus, cell-suspension cultures, root culture, shoot tip and meristem culture, embryo culture,
microspore culture.
Unit 5: Tissue Culture as some of Genetic Variability Somaclonal and gametoclonal variation, Selection,
Sources and causes of variation, Application in crop improvement. Tissue culture in Production of Haploids
and methods of diplodization, triploids, embryo rescue and development of virus free plants
Plant Tissue Culture: Applications and Limitations. S.S. Bhojwani (1990), Elsevier, Amsterdam.
Micropropagation: P.C. Debergh and R.H. Zimmerman (1990), Kluwer Academic Publ. Dordrecht.
Transgenic plants Lindsey and Jones
Plants, genes & crop improvement, Crispeels ASPB, 2002
Agricultural Biotechnology A. Altman.
65
DATA STRUCTURE AND ALGORITHMS
Performance analysis of Algorithm, time complexity, Big-oh notation, Elementary data organization data
structure operations, Recurrences, Arrays, Operation on arrays, representation of arrays in memory,
single dimensional and multidimensional arrays, spare matrices, Character storing in C, String operations.
Stack operation, PUSH and POP, Array representation of stacks, Operation associated with stacks
Application of stacks, Recursion, Polish experession, Representation quesuesopration on quesues, Priority
quesuesDquesues, Singly and circularly linked list, List operations Lists implementations.
Unit-III: Tree
Basic terminology, Binary Trees, Binary tree representation, Algebraic/expressions, Complete Binary
Trees, Extended binary tree, representing binary tress in memory, linked representation of Binary trees,
Traversing binary trees & Searching in binary trees, Inserting in binary search trees, Complexity of
searching algorithm, Heaps, general trees, Threaded binary tree.
Unit-IV: Graphs
Linear search, binary Search, Internal and External sorting, Bubble sorting, selection sort, Insertion sort,
quick sort, Two way merge sort, Heap sort, sorting on different keys, practical consideration for internal
sorting, External Sorting, Storage Devices : Magnetic tapes, Disk Storage, Sorting with disks and
Indexing techniques, introduction to B tree and B+ tree, File organization and storage management,
Introduction to hoisting.
Text:
1. Data Structures and Algorithms, A.V. Aho, J.E. Hopcroft and J. Ullman, Addison-
Wesley Publishing
2. Database Design, Development and Deployment with Student CD, P. Rob and E. Semaan, McGraw-
Hill/Irwin
3. Schaums Outline of Data Structures with C++, J.R. Hubbard, McGraw Hill Trade.
References:
1.Database system concepts, A. Silberschatz, P.B. Galvin and G. Gagne, John Wiley and Sons Inc.
2. Introduction to Data Structures and Application, J. Tremblay and P.G. Sorensen, McGraw
Hill College Division
66
Bioprocess Engineering
Unit I
Methods of inoculation and medium preparation, media design and optimization. Microbial growth in
closed, semi-open and open cultivation systems. Maintenance energy and yield concepts, parameters of
growth and analysis of growth data, estimation of biomass.
Unit II
Sterilization: concept and methods. Sterilization of medium. Kinetics of thermal death of microorganisms.
Batch sterilization. Continuous sterilization. Sterilization of air: Methods, filters and design of depth filters.
Unit III
Microbial kinetics of growth and substrate utilization. Product formation in batch, plug flow and chemostat
culture., Microbial pellet formation. Flocculation. Kinetics and dynamics of pallet formation.
Unit IV
Material and energy balance in steady and unsteady reaction systems. Oxygen transfer in bioreactors,
measurement of KLa. Oxygen transfer in large vessels. Control of physical, chemical and biological
environment of the bioreactor. Advanced control strategies viz. PID controllers, fuzzy logic based
controllers and artificial neural network based controllers, Role of physical, chemical & biological sensors.
67
INDUSTRIAL COMMUNICATION-IV
Objective: The Module improves ability to interact, share and respond it helps to overcome stage fear and
command over language.
68
FOOD TECHNOLOGY LAB
Course Contents:
Module I: Estimation of ascorbic acid from given food sample by titrimetric method.
69
CELL AND TISSUE CULTURE TECHNIQUES LAB
List of experiments:
Module II: Preparation of stock solutions of MS (Murashige & Skoog, 1962) basal medium and plant
growth regulator stocks.
Module IV: Preparation and sterilization of explants and inoculation of explants on culture media.
Module V: Embryo Culture. To isolate embryos of Cicer aertinum and perform in vitro culture.
70
BIOETHICS, BIOSAFETY & IPR
Unit I
Jurisprudential definition and concept of property, rights, duties and their correlation. History and evolution
of IPR- like patent design and copy right, Indian patent act 1970 (amendment 2000), international
convention in IPR, major changes in Indian patent system as post TRIPS effects:
Unit II
Distinction among various forms of IPR, Requirement of a patentable novelty, invention step and prior art
and state of art, procedure.
Unit III
Unit IV
Detailed information on patenting biological products, Biodiversity, Budapest treaty, Appropriate case
studies.
Unit V
Recommended Books:
1. Patent Strategy For Researches & Research Manegers- Knight, Wiley Publications.
2. Agriculture & Intellectual & Property Rights, V. Santaniello& R E Evenson, University Press.
3. Intellectual Property Protection & Sustainable Development, Phillipe Cullet,
LdexixNexis Butterworths.
4. Biotechnology & Safety Assessment, Thomas, Ane/Rout Publishers.
5. Biotechnology in Comparative Perspective, Fuchs, Ane/Rout Publishers.
71
ENZYMOLOGY AND ENZYME TECHNOLOGY
UNIT I: Enzymes
Single substrate steady state kinetics; MichaelisMenten equation, Linear plots, King-Altmans method;
Inhibitors and activators; Multisubstrate systems; ping-pong mechanism, Alberty equation, Sigmoidal
UNIT III
Reactors for batch/continuous enzymatic processing, choice of reactor type; idealized enzyme reactor
systems, Mass transfer in enzyme reactors: Steady state analysis of mass transfer and biochemical reaction
in enzyme reaction.
Enzyme catalysis in organic media; catalytic antibodies and non protein biomolecules as catalysts,
Text:
Biotechnological Innovations in Chemical Synthesis, R.C.B. Currell, V.D. Mieras, Biotol Partners Staff,
Butterworth Heinemann.
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Enzymes: A Practical Introduction to Structure, Mechanism and Data Analysis, R.A. Copeland, John
References:
Enzyme Kinetics: Behaviour and Analysis of Rapid Equilibrium and Steady State Enzyme Systems, I.H.
Segel, Wiley-Interscience.
Industrial Enzymes & their applications, H. Uhlig, John Wiley and Sons Inc.
73
IMMUNOLOGY AND IMMUNOTECHNOLOGY
Course Contents:
Unit I: Introduction
Overview of Immune system: History and scope of Immunology, Types of immunity: innate, acquired,
Comparative immunity. Immune dysfunction and its consequences.
Cells of the immune system lymphoid cells: B, T and NK cells, Primary lymphoid organs, Secondary
lymphoid organs-lymph nodes, spleen mucosal associated lymphoid tissues.
Generation of B-cell and T- cell response: Antigens: Immunogenicity vs. antigenicity Epitopes (properties
of B-cell and T-cell epitopes)
Basic and fine structure of immunoglobulin: light chains, heavy chains and sequences Antigen determinants
on Immunoglobulin: Isotopic, allotypic, Idiotypic; Immunoglobulin Super family; Immunoglobulin classes
and Biological activity: Immunoglobulin mediated Effectors functions optimization: Activation of
complement; Antibody dependent cell Mediated cytotoxicity. Clinical focus: Passive antibody therapy (IgG,
IgM, IgA, IgE and IgD), hypersensitivity and immunological disorder.
Variable region gene rearrangements; Mechanism of variable region DNS rearrangements: Generations of
antibody diversity; Class switching among constant regions genes; Antibody and genes and antibody
engineering.
Strength of antigen and antibody interactions: Antibody affinity, antibody avidity Cross-reactivity;
Precipitation reactions, agglutination reactions (immunodiffusion and Immuno-electrophoretic technique);
Radioimmunoassay; Enzyme linked Immunosorbant Assay (ELISA); Western Blotting; Immuno
precipitation; Immunofluorenscence; Flow cytometery and Fluorescence.
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Unit VII: MHC-Major Histocompatibility complexes
MHC molecules and genes; Antigen processing and presentation, activation of B and T-Lymphocytes,
cytokines and their role in immune regulation, T cell regulation and MHC Restriction, immunological
tolerance.
Immune response to infectious disease (AIDS, Malaria, Kala azar & TB); Vaccines (whole Organism,
purified macromolecules, recombinant vaccine, synthetic polypeptide etc. Autoimmunity; Hypersensitivity;
Transplantation immunology: graft rejections, graft vs. host Response; Cancer and immune system.
Text:
Basic Immunology, A.K. Abbas and A.H. Lichtman, Saunders W.B. Company
Basic Immunology, A.K. Abbas and A.H. Lichtman, Saunders W.B. Company
References:
Kuby Immunology, R.A. Goldsby, T.J. Kindt, and B.A. Osborne, Free
75
BIOPROCESS TECHNOLOGY
Course Contents:
Unit I
formulation, Cell culture techniques; Inoculum development and aseptic transfers. Different types of pumps,
valves, and line materials, piping conventions etc. used in Biochemical Process.
Unit II
Process technology for the production of primary metabolites, eg.biomass, ethanol, acetone-butanol, citric
Ethanol: production by batch, continuous and cell recycle adopted by various technologies practiced in
Indian distilleries using molasses and grains. Computation of fermentation efficiency, distillation efficiency
and overall efficiency of ethanol production, recovery, uses, glucose effect etc.Power alcohol definition,
Lysine: Indirect and direct fermentation mechanism of ph of metabolic block in accumulation of L- lysine
Biomass: Bakers and distillers yeast production using various raw materials, bios factors for growth,
Crabtree effect, harvesting, different forms and uses. What are mushroom, different forms of common
mushroom production from agro based raw materials and uses. Biofertilizers, biocompost and Biopesticides.
Unit III
Penicillin: Classification, various penicillin as precursor and R side chain, penicillinase, 6-APA,
fermentation etc.
Tetracycline: chemical structure, production, harvest and recovery, use by-product of tetracycline
fermentation etc.
Unit IV
Microbial production of industrial enzymes glucose isomerase, penicillin acylase, cellulase, amylase,
Bioprocess Engineering Basic Concepts, M.L. Shuler and F. Kargi, Prentice Hall
Bioprocess Engineering, B.K. Lydersen, K.L. Nelson, B.K. Lyderson and N. DElia, John Wiley and
Sons Inc.
77
BIOMEDICAL INSTRUMENTATION
Unit I
Unit II
The Cardiovascular system, Cardiovascular measurements, Patient care and monitoring, Measurements
in the respiratory system.
Unit III
Noninvasive diagnostic instrumentation, the nervous system, Instrumentation for sensory measurements and
the study of behaviours, Biotelemetry.
Unit IV
Unit V
Recommended books:
78
INDUSTRIAL COMMUNICATION-V
Objective The Module provide the ways and means of enhancing innovative ideas and providing
solutions and enhance employability.
79
ENZYMOLOGY AND ENZYME TECHNOLOGY LAB
Course Code: EBT 522 Credit Units: 01
Course Contents:
List of experiments:
Module II: Enzyme assay; activity and specific activity determination of amylase, nitrate
Module IV: Enzyme Kinetics: Effect of varying substrate concentration on enzyme activity,
Lineweaver-Burk plot.
80
IMMUNOLOGY AND IMMUNOTECHNOLOGY LAB
List of experiments:
81
BIOPROCESS TECHNOLOGY LAB
Course Code: EBT 524 Credit Units: 01
List of experiments:
Module II: Determination of Thermal Death Point and Thermal death time of microorganisms for design of
a sterilizer
Module III: Determination of growth curve of a supplied microorganism and also determines substrate
degradation profile and to compute specific growth rate and growth yield from the data
obtained.
Module V: Isolation and screening of industrially important microorganism (Bacillus subtilis) from soil
sample.
Module VI: Production of l-Asparaginase by Bacillus subtilis and estimation of protein concentration by
ninhydrin test.
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DOWN STREAM PROCESSING
Unit I
Unit II
Primary separation and Recovery Process: Cell, Disruption methods(Physical,chemical and Enzymatic) for
intracellular prducts.Removal of insoluble, biomass. Separation techniques Flocculation, Sedimentation,
Centrifugation and Filtration..
Unit III
Product Isolation - Extraction and Adsorption methods, Solid- Liquid , Liquid -liquid Separation,
Distillation.Enrichment operation-Membrane based Separation -micro and ultrafiltration ( Theory, Design
and Configuration of the Equipment), Precipitation methods Ammonium, Sulphate, organic solvents, High
molecular weight polymers.
Unit IV
Product Purification: Electrophoresis and Chromatography principle for product purification, Different
Electrophoresis technique Isoelectric, focussing ,chromatographic technique with special reference to ion-
exchange ,affinity, GLC, HPLC.
Unit V
Product Polishing: Crystallization, Drying. A Few case studies: Citric acid, Glutamic acid, Penicillin
G,Extracellular Enzymes, Intracellular enzymes, Antibodies.
Recommended Books:
1. Comprehensive biotechnology- Murray Moo-Young, Vol. II-latest ed., Pergan Publishers.
2. H. J. Rehm and G. Reed, Biotechnology- Vol. 3, 4, 5, Verlag Publishers
3. Stanbury&Whitteker, Principles Of Fermentation Technology, Pergamon Press
4. A Biologists Guide to Principles & Techniques of Practical Biochemistry- Wilson and
Golding, Cambridge University Press
5. Humphrey, Aiba& Miller, Biochemical Engg., Academic Press.
6. Biochemical Engg. -Bailly&Ollis, Academic Press.
7. Product Recovery in Bioprocess Technology, Heinemann, Butterworth Publication.
8. Electrophoresis in Practice, Westermeier- Wiley Publications.
9. Handbook of Analytical techniques-H. Gunzler, Wiley Publications.
10. International Methods of Analysis- Willard et al., CBS Publication.
11. Bioseparation Techniques, Sivasankar, Prentice-Hall of India, 2004.
12. Bioseparations (Principles & techniques), B. Sivasankar, Prentice-Hall of India, 2005.
13. Citric Acid Biotechnology, Linden, Ane/Rout Publishers.
83
DATABASE MANAGEMENT SYSTEMS
Course Objective:
It enables the students to access biological information networks and databases in order to understand the
different techniques of biotechnology to build detection systems especially in the prevention and treatment
of human diseases.
Course Contents:
Functional dependencies, Normal forms; Concurrency control and database discovery -concept of
transaction: atomicity, consistency, isolation and durability, transactions and schedules, concurrent
execution of transactions, Lock based concurrency control, Database recovery
Unit V: Current trends
Text:
Data Mining: Concept and techniques, J. Han and M. Kamber, Morgan Kaufman.
Data Mining, A.K. Pujari, Sangam Books Ltd.
Database Management, P.C. Desai.
Introduction to Database Systems, C.J. Date, Addison Wesley Publishing.
References:
Principles of Database and Knowledge Based systems, J.D. Ullman, Computer Science Press.
The Data Warehouse Lifecycle Toolkit, John Wiley and Sons Inc.
84
GENETIC ENGINEERING
Unit-II: Molecular tools and their applications: DNA & RNA modifying enzymes: Restriction enzymes and
other endonucleases, Exonucleases, Polymerases, Kinases, Methylases and Ligases.
Unit-III: Molecular Techniques: Restriction analysis of DNA, Restriction map, electrophoretic techniques
for nucleic acid protein analyses, DNA sequencing, Chemical synthesis of oligonucleotides, Southern,
Northern and Western blotting techniques.
Unit-IV: Different vectors for molecular cloning: Plasmids, Bacteriophages, Phagemids, Cosmids; YAC
and BAC, Transformation Techniques
Unit-V: Construction of genomic and cDNA libraries; Gene specific probes; Screening strategies for
isolation of genes.
Unit-VI: Alternative strategies of gene cloning: PCR techniques and their applications, introduction to two
and three hybrid systems, nucleic acids microarrays.
Unit-VII: Random and site-directed mutagenesis, Expression strategies for heterologous genes: Expression
of recombinant proteins in Bacteria, Yeast, Insect cells, Mammalian and Plant cells, in vitro transcription
and translation.
1. Principles of Gene Manipulation and Genomics (7th edition), by S.B. Promorose and R. M. Twyman,
Blackwell Publishing (2006)
2. Gene Cloning and DNA Analysis: An Introduction (6th edition), by T A Brown, Wiley Blackwell
Publications.
3. Recombinant DNA by Watson.J.D. et al, 1993, Scientific American Books, New York.
Unit V
Bioethical issues related to animal biotechnology.
86
STATISTICS FOR BIOLOGY
Course Contents:
Unit I
Unit II
Probability: Random Experiments, Trial and Event, Sample Space, Mutually Exclusive or Disjoint Events,
Mutually Exhaustive Events, Equally Probable Events, Complementary Event, Classical definition of
Bivariate distribution Correlation, Types of Correlation, Simple Correlation Coefficient for ungrouped data,
Standard Error, Probable error of Correlation Coefficient. Rank correlation, Some examples.
Unit V: Regression
Definition, Regression lines and Regression Coefficients, Properties of Regression Coefficients, Some
87
Unit VI: Introduction to the following Statistical terms
Parameter, Statistic, Null hypothesis, Alternative hypothesis, Critical region, Type1 Error, Type 11 Error,
Level of significance, P-value and its applications. Test of Significance for Small samples: One sample t-
test, Paired t-test, Degrees of freedom for t-test, F test for equality of Population variances, Degrees of
freedom for F-test. Test of Significance for Large samples: Normal test for sample mean and population
mean, Normal test for two sample means, Chi-square Test: Test of goodness of fit, Test of Independence of
attributes, Degrees of freedom for Chisquare test, Coefficient of contingency, Yates correction for
continuity. Analysis of Variance: One way and Two way (only Examples)
Text:
Introduction to Biostatistics, Ronald N. Forthfer and Eun Sun Lee, Publisher: Elsevier.
References:
Biostatistics: A manual of Statistical Methodology for use in Health, Nutrition and Anthropology, K.
Fundamentals of Mathematical Statistics, S.C. Gupta and V.K. Kapoor, Publisher: S.Chand&
Co. Statistical Analysis, Kaushal, T.L. Publisher: Kalyani Publishers
Mathematical Statistics, H.C. Saxena, and V.K. Kapoor: S. Chand & Company
Biostatistics, P.N. Arora and P.K. Malhan, Publisher: Himalaya Publishing House.
88
INDUSTRIAL COMMUNICATION-VI
Objective This Module is a combination of Technical paper writing and literature features
89
DOWNSTREAM PROCESSING LAB
The laboratory will help the students to extract different bioproducts during their characterization since
any of these products may be of biotechnological importance. They can be exploited in exploring the
future biotechnology.
Course Contents:
Module I
Module II
Module III
Module IV
Module V
Module VI
Electrophoresis.
90
DATABASE MANAGEMENT LAB
Module I
Module II
Module III
Implementation of selection, projection and joins (internal and external) with SQL and Perl.
Module IV
Module V
Module VI
91
BIOTECHNOLOGY IN HEALTH CARE
Course Contents:
Unit I
Unit II
Unit III
Unit IV
Unit V
Drug Targeting Organ-Specific Strategies: Basic concepts and novel advances, Brain-specific drug
targeting strategies, Pulmonary drug delivery, Cell specific drug delivery.
1. Pharmaceutical Chemistry by Christine M. Bladon. John Wiley & Sons, Ltd. (2002).
2. Burgers Medicinal Chemistry and Drug Discovery (5th edition) by Manfred E. Wolff. A Wiley & Sons,
Inc. (2000).
3. Drug Targeting Organ-Specific Strategies by Grietje Molema and Dirk K. F. Meijer. Wiley-VCH. (2002).
92
CHEMICAL ENGINEERING PRINCIPLES
Course Contents:
Units and dimensions, Dimensional analysis; Simple problems on material balance calculations involving
unit.processes and reactive systems; Available electron balances. Basic energy concepts -enthalpy changes
in chemical/biochemical reactions and in non-reactive processes, Energy balance calculations, Use of Steam
Kinetics of homogenous reactions: Concepts of reaction rate, order of reaction and molecularity, Analysis
of batch reactors for kinetic interpretation of data and isothermal reactor design for single and multiple
Principles of measurement: error, accuracy and sensitivity; Measurement of flow, pressure, temperature,
level, pH, viscosity and chemical composition. Basic concepts of feedback control, control loop and its
elements, Dynamic behaviour of first, second and higher order physical systems, controller hardware,
choice of controllers and settings. Introduction to advanced control systems: feed forward, cascade and ratio
control.
References:
Chemical Process Control, An introduction to Theory and Practice, G. Stephanopoulos, Prentice Hall Inc.
Chemical Reaction Engineering, O. Levenspiel, John Wiley and Sons Inc.
Elementary Principles of Chemical Processes, R.M. Felder and R.W. Rousseau, John Wiley and Sons Inc.
Fundamentals of Chemical Reaction Engineering, C.D. Holland and R.G. Anthony, Prentice Hall Inc.
Process Modelling, Simulation and Control for Chemical Engineers, W.L. Luyben, McGraw Hill
93
THERMODYNAMICS OF BIOLOGICAL SYSTEMS
Course Objective:
The main aim is to understand the basis of theories related to combustion and dissolution. It will help in the
study of heat evolution, control of dissipation and will suggest ways for solving the rate phenomena through
Course Contents:
Unit I
Unit II
Unit III
Thermodynamics systems: equilibrium, activity coefficients and phase equilibrium functions of state, cyclic
processes, work, energy and metabolic heat; Mechanical equivalent of heat, energy as a function of state.
Second law; Reversible vs. irreversible processes entropy, work; Combination of the first and second
law, Free energy, useful work and delta G. Entropy: Ideality and Molecular Cohesion, Probabilistic
Failure of classical thermodynamics in describing biological processes, Standard free energy changes
Visualization of the potential velocity and steady flow; Ficks law and diffusion. Local Equilibria and
steady state: Energy vs. Power; Transducers in biological states, Constitutive equations, Dynamic efficiency
and Onsager (nonequilibrium thermodynamics), Prigogines principle, Spontaneous coupling and entropy
production.
94
Unit VII: Non-equilibrium thermodynamics
Reversible work, exact differentials and function of state, first and second law, The electrochemical
potential, External forces an steady state, Ficks Law, chemical reactions in the steady state, internal
entropy production, cells as non-equilibrium stationary states; Diffusion and membrane transport.
ordering in time and space far from equilibrium, glycolytic oscillations, biological clocks, routes to chaos.
Physical Chemistry: Principles and Applications in Biological Sciences, I. Tincoco, K.Sauer and
Physical Chemistry for the Chemical and Biological Sciences, R. Chang, University Science Books
Thermodynamics and Kinetics for the Biological Sciences, G.G. Hammes, John Wiley and Sons Inc
95
CURRENT TOPICS IN BIOTECHNOLOGY
Course Contents:
Unit I: Bioremediation
Introductory concept, Bioremediation principles, mechanism for Bioremediation of air, water and soil
Genetically modified food crops, food animals examples and mode of production, future goals in GM
food crops and animals, scientific evaluation of public concerns, legal requirements in production of GMO,
Gene mutation, point mutation, allele specific oligonucleotides, ARMS, oligonucleotide ligation,
disease diagnosis with linked genetic markers, fluorescently labeled DNA sequencing.
Introduction, definition, hybrid nanopracticles, smrat drug delivery, gene sensors, biomolecule
Definition, properties, proliferation, culture of stem cells, medical applications of stem cells, ethical
Types of cancer, development, causes and properties of cancer, viruses (Hepatitis B & C, SV4U, polymo
96
Unit VII: Forensic Biotechnology
MLP, SLP technology, PCR technology in crime detection, STR and databases, mitochondrial DNA and Y
chromosome analysis in forensic science, DNA chip technology, role of molecular biology and
The Cell A molecular Approach, 3rd Edn, Geoffrey M. Cooper and Robert E. Hausman, ASM Press
Molecular Biology and Biotechnology, 4th Edn, J.M Walker and R. Rapley, Panima Books
Stem Cell Biology, Daniel Marshak, Richard L. Gardener and David Gottlieb, Cold Spring Harbour
Laboratory Press
Environmental Microbiology, 2nd Edition, Ian L. Pepper and Charles P. Gerba, Elsevier Pub.
Environmental Biotechnology Concepts and Application, Hans Joachim Jordening and Jesefwinter
Wiley VCH
97
ENVIRONMENTAL BIOTECHNOLOGY
Introduction
Ecology and ecosystem.
Module V: Biodegradation
Biodegradation of xenobiotic compounds, organisms involved in degradation of chlorinated
hydrocarbons, substituted simple aromatic compounds, polyaromatic hydrocarbons, pesticides, surfactants
and microbial treatment of oil pollution
99
PRINCIPLES OF MANAGEMENT AND
ENTREPRENEURSHIP DEVELOPMENT
Course Objective:
The Management and Entrepreneurship program is designed to prepare students for an exciting career in
todays competitive era. The course will equip students with the knowledge to cope up with the changing
environment because of the advent of technology and other influences. The course will also develop
required entrepreneurship skills in the students from a variety of disciplinary perspectives known to be
Course Contents:
Module I
Principles and function of management, Planning and decision making, Line and staff
Module II
Module III
Entrepreneurship and entrepreneurial process, Business plan, Form of ownership suitable for business.
Module IV
development programme.
Text:
References:
100
MARKETING & MANAGEMENT OF BIOTECHNOLOGY
Course Objective:
1. Why there is a need to commercialize biotechnology. Discovery, market needs, development process,
2. Creating and marketing the image of the biotechnology Company. Art of negotiation & effective
communication.
3. Role of venture capitalism, business plan, selection of CEO and personnel, real estate for a biotech start-
up.
4. How to portray management and role of a biotechnology manager, technology decisionmaking, and
5. Role of Research & development University-industry technology transfer arrangements, how and why a
6. Positioning, power and importance of positioning of a company name and product, Workable marketing
7. Effective advertising and marketing. Opportunities international, marketing and lessons to be learned.
8. Indian and foreign prospective of biotechnology, and current challenges for thebiotechnology based
products.
2. Biotechnology: The science & the business by V. Moser & R.E. Cape (1999) Harwood.
102
103